Restriction map for
pF1KB7959
(Circular) (Six-base) MAPSORT of: pF1KB7959.seq Check: 380 from: 1 to: 3905
pF1KB7959 3905 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
AflIII A'CryG_T
Cuts at: 235 1257 2361 3338 235
Size: 1022 1104 977 802
Fragments arranged by size:
1104 1022 977 802
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AleI CACnn'nnGTG
Cuts at: 227 860 227
Size: 633 3272
AlwNI CAG_nnn'CTG
Cuts at: 414 585 720 1210 2777 414
Size: 171 135 490 1567 1542
Fragments arranged by size:
1567 1542 490 171 135
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 364 403 909 3258 3514 364
Size: 39 506 2349 256 755
Fragments arranged by size:
2349 755 506 256 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 658 925 658
Size: 267 3638
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 787 921 1335 1583 1618 787
Size: 134 414 248 35 3074
Fragments arranged by size:
3074 414 248 134 35
BanII G_rGCy'C
Cuts at: 340 919 1949 340
Size: 579 1030 2296
Fragments arranged by size:
2296 1030 579
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbsI GAAGACnn'nnnn_
Cuts at: 848 848
Size: 3905
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglI GCCn_nnn'nGGC
Cuts at: 326 326
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 181 1017 181
Size: 836 3069
Bme1580I G_kGCm'C
Cuts at: 579 857 1530 1623 2679 579
Size: 278 673 93 1056 1805
Fragments arranged by size:
1805 1056 673 278 93
BmgBI CAC'GTC
Cuts at: 225 225
Size: 3905
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 684 2272 684
Size: 1588 2317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1081 1960 2452 2750 421
Size: 660 879 492 298 1576
Fragments arranged by size:
1576 879 660 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 671 701 671
Size: 30 3875
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 330 787 330
Size: 457 3448
BseYI C'CCAG_C
Cuts at: 198 353 707 813 1330 2088 2665 3161 198
Size: 155 354 106 517 758 577 496 942
Fragments arranged by size:
942 758 577 517 496 354 155 106
BsiEI CG_ry'CG
Cuts at: 104 326 390 1447 1493 2701 104
Size: 222 64 1057 46 1208 1308
Fragments arranged by size:
1308 1208 1057 222 64 46
BsiHKAI G_wGCw'C
Cuts at: 224 608 919 1697 1887 2679 224
Size: 384 311 778 190 792 1450
Fragments arranged by size:
1450 792 778 384 311 190
Bsp1286I G_dGCh'C
Cuts at: 224 340 579 608 857 919 1530 1623 1697
Size: 116 239 29 249 62 611 93 74
Cuts at: 1697 1887 1949 2679 224
Size: 190 62 730 1450
Fragments arranged by size:
1450 730 611 249 239 190 116 93
74 62 62 29
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 606 636 955 1169 1471 1852 606
Size: 30 319 214 302 381 2659
Fragments arranged by size:
2659 381 319 302 214 30
BsrBI CCG'CTC
Cuts at: 50 655 2197 2251 3735 3900 50
Size: 605 1542 54 1484 165 55
Fragments arranged by size:
1542 1484 605 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 261 325 771 790 1903 2084 2293 261
Size: 64 446 19 1113 181 209 1873
Fragments arranged by size:
1873 1113 446 209 181 64 19
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstEII G'GTnAC_C
Cuts at: 648 648
Size: 3905
BstYI r'GATC_y
Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111
Size: 929 467 23 335 246 1012 86 12
Cuts at: 3111 3457 1
Size: 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 131 131
Size: 3905
BtgI C'CryG_G
Cuts at: 228 746 228
Size: 518 3387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 323 458 1490 1664 2055 2082 3427 323
Size: 135 1032 174 391 27 1345 801
Fragments arranged by size:
1345 1032 801 391 174 135 27
EagI C'GGCC_G
Cuts at: 323 1490 323
Size: 1167 2738
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 134 835 134
Size: 701 3204
EcoO109I rG'GnC_Cy
Cuts at: 287 582 717 1044 287
Size: 295 135 327 3148
Fragments arranged by size:
3148 327 295 135
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 141 1924 141
Size: 1783 2122
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 1339 1587 2609 3588 1339
Size: 248 1022 979 1656
Fragments arranged by size:
1656 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 197 229 468 500 671 703 1747 1777 1779
Size: 32 239 32 171 32 1044 30 2
Cuts at: 1779 1809 197
Size: 30 2293
Fragments arranged by size:
2293 1044 239 171 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 235 3338 235
Size: 3103 802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 702 875 2576 2760 702
Size: 173 1701 184 1847
Fragments arranged by size:
1847 1701 184 173
MscI TGG'CCA
Cuts at: 460 1666 460
Size: 1206 2699
MslI CAynn'nnrTG
Cuts at: 119 227 529 860 1894 2021 119
Size: 108 302 331 1034 127 2003
Fragments arranged by size:
2003 1034 331 302 127 108
MspA1I CmG'CkG
Cuts at: 151 702 711 1016 1330 1690 2703 2948 151
Size: 551 9 305 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 551 360 314 305 245 9
NaeI GCC'GGC
Cuts at: 263 327 773 2086 263
Size: 64 446 1313 2082
Fragments arranged by size:
2082 1313 446 64
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NgoMIV G'CCGG_C
Cuts at: 261 325 771 2084 261
Size: 64 446 1313 2082
Fragments arranged by size:
2082 1313 446 64
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PpuMI rG'GwC_Cy
Cuts at: 287 582 287
Size: 295 3610
PstI C_TGCA'G
Cuts at: 164 716 758 952 1637 164
Size: 552 42 194 685 2432
Fragments arranged by size:
2432 685 552 194 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 702 711 1330 1690 702
Size: 9 619 360 2917
Fragments arranged by size:
2917 619 360 9
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SanDI GG'GwC_CC
Cuts at: 287 287
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 160 712 754 948 1633 2626 2817 33
Size: 127 552 42 194 685 993 191 1121
Fragments arranged by size:
1121 993 685 552 194 191 127 42
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 436 1060 1975 2467 2729 436
Size: 624 915 492 262 1612
Fragments arranged by size:
1612 915 624 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
StyI C'CwwG_G
Cuts at: 209 836 1039 1393 209
Size: 627 203 354 2721
Fragments arranged by size:
2721 627 354 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 1488 1621 2080 2289 883
Size: 605 133 459 209 2499
Fragments arranged by size:
2499 605 459 209 133
TatI w'GTAC_w
Cuts at: 497 3420 497
Size: 2923 982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 688 720 3422 3454 688
Size: 32 2702 32 1139
Fragments arranged by size:
2702 1139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 880 1400 880
Size: 520 3385
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
XmnI GAAnn'nnTTC
Cuts at: 124 124
Size: 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI