Restriction map for
pF1KB7955
(Circular) (Six-base) MAPSORT of: pF1KB7955.seq Check: 1397 from: 1 to: 3881
pF1KB7955 3881 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 303 303
Size: 3881
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
AflIII A'CryG_T
Cuts at: 153 1233 2337 3314 153
Size: 1080 1104 977 720
Fragments arranged by size:
1104 1080 977 720
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 3881
AlwNI CAG_nnn'CTG
Cuts at: 1186 2753 1186
Size: 1567 2314
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 231 469 885 3234 3490 231
Size: 238 416 2349 256 622
Fragments arranged by size:
2349 622 416 256 238
AseI AT'TA_AT
Cuts at: 20 20
Size: 3881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 614 901 614
Size: 287 3594
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 320 353 320
Size: 33 3848
BamHI G'GATC_C
Cuts at: 906 906
Size: 3881
BanI G'GyrC_C
Cuts at: 897 1311 1559 1594 897
Size: 414 248 35 3184
Fragments arranged by size:
3184 414 248 35
BanII G_rGCy'C
Cuts at: 895 1925 895
Size: 1030 2851
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 1401 1401
Size: 3881
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3881
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 993 993
Size: 3881
Bme1580I G_kGCm'C
Cuts at: 1506 1599 2655 1506
Size: 93 1056 2732
Fragments arranged by size:
2732 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 712 1093 1174 1509 3653 712
Size: 381 81 335 2144 940
Fragments arranged by size:
2144 940 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
Bpu10I CC'TnA_GC
Cuts at: 361 361
Size: 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1936 2428 2726 1057
Size: 879 492 298 2212
Fragments arranged by size:
2212 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 667 3585 667
Size: 2918 963
BsaAI yAC'GTr
Cuts at: 1234 1234
Size: 3881
BsaBI GATnn'nnATC
Cuts at: 6 1395 3432 6
Size: 1389 2037 455
Fragments arranged by size:
2037 1389 455
BsaHI Gr'CG_yC
Cuts at: 1312 1560 2262 3758 1312
Size: 248 702 1496 1435
Fragments arranged by size:
1496 1435 702 248
BsaWI w'CCGG_w
Cuts at: 810 1070 1591 2269 2543 2690 3101 810
Size: 260 521 678 274 147 411 1590
Fragments arranged by size:
1590 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 116 146 116
Size: 30 3851
BseYI C'CCAG_C
Cuts at: 305 795 1306 2064 2641 3137 305
Size: 490 511 758 577 496 1049
Fragments arranged by size:
1049 758 577 511 496 490
BsiEI CG_ry'CG
Cuts at: 104 1423 1469 2677 104
Size: 1319 46 1208 1308
Fragments arranged by size:
1319 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 895 1673 1863 2655 895
Size: 778 190 792 2121
Fragments arranged by size:
2121 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 895 1506 1599 1673 1863 1925 2655 895
Size: 611 93 74 190 62 730 2121
Fragments arranged by size:
2121 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3101 3101
Size: 3881
BspHI T'CATG_A
Cuts at: 3057 3057
Size: 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 303 931 1145 1447 1828 303
Size: 628 214 302 381 2356
Fragments arranged by size:
2356 628 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2173 2227 3711 3876 50
Size: 2123 54 1484 165 55
Fragments arranged by size:
2123 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 1793
Size: 3881
BsrFI r'CCGG_y
Cuts at: 1879 2060 2269 1879
Size: 181 209 3491
Fragments arranged by size:
3491 209 181
BssSI C'ACGA_G
Cuts at: 2152 2510 2152
Size: 358 3523
BstAPI GCAn_nnn'nTGC
Cuts at: 1258 1258
Size: 3881
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstXI CCAn_nnnn'nTGG
Cuts at: 803 803
Size: 3881
BstYI r'GATC_y
Cuts at: 1 906 1373 1396 1731 1977 2989 3075 3087
Size: 905 467 23 335 246 1012 86 12
Cuts at: 3087 3433 1
Size: 346 449
Fragments arranged by size:
1012 905 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 196 196
Size: 3881
BtgI C'CryG_G
Cuts at: 107 107
Size: 3881
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 564 1831 2012 2175 564
Size: 1267 181 163 2270
Fragments arranged by size:
2270 1267 181 163
BtsI GCAGTG_nn'
Cuts at: 185 1124 185
Size: 939 2942
DraI TTT'AAA
Cuts at: 880 880
Size: 3881
DraIII CAC_nnn'GTG
Cuts at: 480 480
Size: 3881
DrdI GACnn_nn'nnGTC
Cuts at: 1587 2445 1587
Size: 858 3023
EaeI y'GGCC_r
Cuts at: 1466 1640 2031 2058 3403 1466
Size: 174 391 27 1345 1944
Fragments arranged by size:
1944 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1466 1466
Size: 3881
EarI CTCTTCn'nnn_
Cuts at: 670 1904 2114 670
Size: 1234 210 2437
Fragments arranged by size:
2437 1234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 2399 2545 3691 2089
Size: 310 146 1146 2279
Fragments arranged by size:
2279 1146 310 146
EcoICRI GAG'CTC
Cuts at: 893 893
Size: 3881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1020 1020
Size: 3881
EcoRI G'AATT_C
Cuts at: 885 885
Size: 3881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3881
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 1315 1563 2585 3564 1315
Size: 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 1723 1753 1755 1785 116
Size: 32 1575 30 2 30 2212
Fragments arranged by size:
2212 1575 32 30 30 2
HincII GTy'rAC
Cuts at: 708 920 708
Size: 212 3669
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2736 2552
Size: 184 3697
MscI TGG'CCA
Cuts at: 1642 1642
Size: 3881
MslI CAynn'nnrTG
Cuts at: 158 424 1870 1997 158
Size: 266 1446 127 2042
Fragments arranged by size:
2042 1446 266 127
MspA1I CmG'CkG
Cuts at: 841 992 1306 1666 2679 2924 841
Size: 151 314 360 1013 245 1798
Fragments arranged by size:
1798 1013 360 314 245 151
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 3881
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NcoI C'CATG_G
Cuts at: 107 107
Size: 3881
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 3881
NspI r_CATG'y
Cuts at: 157 212 313 934 1434 2341 157
Size: 55 101 621 500 907 1697
Fragments arranged by size:
1697 907 621 500 101 55
PciI A'CATG_T
Cuts at: 153 2337 153
Size: 2184 1697
PflMI CCAn_nnn'nTGG
Cuts at: 170 170
Size: 3881
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PstI C_TGCA'G
Cuts at: 192 928 1613 192
Size: 736 685 2460
Fragments arranged by size:
2460 736 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 841 1306 1666 841
Size: 465 360 3056
Fragments arranged by size:
3056 465 360
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 895 895
Size: 3881
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
SbfI CC_TGCA'GG
Cuts at: 928 928
Size: 3881
ScaI AGT'ACT
Cuts at: 856 3398 856
Size: 2542 1339
SfcI C'TryA_G
Cuts at: 33 119 188 434 924 1609 2602 2793 33
Size: 86 69 246 490 685 993 191 1121
Fragments arranged by size:
1121 993 685 490 246 191 86 69
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SmaI CCC'GGG
Cuts at: 903 903
Size: 3881
SmlI C'TyrA_G
Cuts at: 1036 1951 2443 2705 1036
Size: 915 492 262 2212
Fragments arranged by size:
2212 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 313 934 1434 313
Size: 621 500 2760
Fragments arranged by size:
2760 621 500
SspI AAT'ATT
Cuts at: 388 388
Size: 3881
StuI AGG'CCT
Cuts at: 201 201
Size: 3881
StyI C'CwwG_G
Cuts at: 107 169 1015 1369 107
Size: 62 846 354 2619
Fragments arranged by size:
2619 846 354 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 1464 1597 2056 2265 319
Size: 1145 133 459 209 1935
Fragments arranged by size:
1935 1145 459 209 133
TatI w'GTAC_w
Cuts at: 854 3396 854
Size: 2542 1339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 3849
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 912 912
Size: 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1376 1376
Size: 3881
XmaI C'CCGG_G
Cuts at: 901 901
Size: 3881
XmnI GAAnn'nnTTC
Cuts at: 760 760
Size: 3881
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI