Restriction map for
pF1KB7954
(Circular) (Six-base) MAPSORT of: pF1KB7954.seq Check: 7570 from: 1 to: 4298
pF1KB7954 4298 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1336 1336
Size: 4298
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 812 821 1076 1141 1255 2123 2555 3302
Size: 126 9 255 65 114 868 432 747
Cuts at: 3302 3570 686
Size: 268 1414
Fragments arranged by size:
1414 868 747 432 268 255 126 114
65 9
AflII C'TTAA_G
Cuts at: 379 379
Size: 4298
AflIII A'CryG_T
Cuts at: 410 1650 2754 3731 410
Size: 1240 1104 977 977
Fragments arranged by size:
1240 1104 977 977
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 346 874 1603 3170 346
Size: 528 729 1567 1474
Fragments arranged by size:
1567 1474 729 528
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 511 708 944 957 1302 3651 3907 511
Size: 197 236 13 345 2349 256 902
Fragments arranged by size:
2349 902 345 256 236 197 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 1318 1318
Size: 4298
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 722 755 722
Size: 33 4265
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 883 1314 1728 1976 2011 883
Size: 431 414 248 35 3170
Fragments arranged by size:
3170 431 414 248 35
BanII G_rGCy'C
Cuts at: 198 1312 2342 198
Size: 1114 1030 2154
Fragments arranged by size:
2154 1114 1030
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BbsI GAAGACnn'nnnn_
Cuts at: 1261 1261
Size: 4298
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1410 1410
Size: 4298
Bme1580I G_kGCm'C
Cuts at: 1923 2016 3072 1923
Size: 93 1056 3149
Fragments arranged by size:
3149 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1510 1591 1926 4070 1510
Size: 81 335 2144 1738
Fragments arranged by size:
2144 1738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1169 2665 1169
Size: 1496 2802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 821 689
Size: 132 4166
Bpu10I CC'TnA_GC
Cuts at: 795 795
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2353 2845 3143 1474
Size: 879 492 298 2629
Fragments arranged by size:
2629 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4002 4002
Size: 4298
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 662 692 1193 1223 662
Size: 30 501 30 3737
Fragments arranged by size:
3737 501 30 30
BseYI C'CCAG_C
Cuts at: 216 1723 2481 3058 3554 216
Size: 1507 758 577 496 960
Fragments arranged by size:
1507 960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1840 1886 3094 104
Size: 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 198 1312 2090 2280 3072 198
Size: 1114 778 190 792 1424
Fragments arranged by size:
1424 1114 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 198 1312 1923 2016 2090 2280 2342 3072 198
Size: 1114 611 93 74 190 62 730 1424
Fragments arranged by size:
1424 1114 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 145 1348 1562 1864 2245 145
Size: 1203 214 302 381 2198
Fragments arranged by size:
2198 1203 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2590 2644 4128 4293 50
Size: 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1049 2210 1049
Size: 1161 3137
BsrFI r'CCGG_y
Cuts at: 2296 2477 2686 2296
Size: 181 209 3908
Fragments arranged by size:
3908 209 181
BsrGI T'GTAC_A
Cuts at: 495 495
Size: 4298
BssSI C'ACGA_G
Cuts at: 149 2569 2927 149
Size: 2420 358 1520
Fragments arranged by size:
2420 1520 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4298
BstYI r'GATC_y
Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504
Size: 1322 467 23 335 246 1012 86 12
Cuts at: 3504 3850 1
Size: 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 845 1883 2057 2448 2475 3820 845
Size: 1038 174 391 27 1345 1323
Fragments arranged by size:
1345 1323 1038 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 768 1136 1160 2321 2531 768
Size: 368 24 1161 210 2535
Fragments arranged by size:
2535 1161 368 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 196 1310 196
Size: 1114 3184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 689 812 821 1076 1141 1255 2123 2555
Size: 3 123 9 255 65 114 868 432
Cuts at: 2555 3302 3570 686
Size: 747 268 1414
Fragments arranged by size:
1414 868 747 432 268 255 123 114
65 9 3
EcoO109I rG'GnC_Cy
Cuts at: 1437 1437
Size: 4298
EcoRI G'AATT_C
Cuts at: 944 1302 944
Size: 358 3940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 1732 1980 3002 3981 1732
Size: 248 1022 979 2049
Fragments arranged by size:
2049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 411 443 1085 1117 1193 1225 2140 2170 2172
Size: 32 642 32 76 32 915 30 2
Cuts at: 2172 2202 411
Size: 30 2507
Fragments arranged by size:
2507 915 642 76 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1337 1337
Size: 4298
HindIII A'AGCT_T
Cuts at: 133 133
Size: 4298
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 997 1232 2969 3153 997
Size: 235 1737 184 2142
Fragments arranged by size:
2142 1737 235 184
MscI TGG'CCA
Cuts at: 847 2059 847
Size: 1212 3086
MslI CAynn'nnrTG
Cuts at: 1000 2287 2414 1000
Size: 1287 127 2884
Fragments arranged by size:
2884 1287 127
MspA1I CmG'CkG
Cuts at: 1167 1409 1723 2083 3096 3341 1167
Size: 242 314 360 1013 245 2124
Fragments arranged by size:
2124 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NdeI CA'TA_TG
Cuts at: 335 335
Size: 4298
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NspI r_CATG'y
Cuts at: 414 640 1351 1851 2758 414
Size: 226 711 500 907 1954
Fragments arranged by size:
1954 907 711 500 226
PciI A'CATG_T
Cuts at: 410 2754 410
Size: 2344 1954
PflMI CCAn_nnn'nTGG
Cuts at: 979 979
Size: 4298
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PsiI TTA'TAA
Cuts at: 1069 1069
Size: 4298
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 731 763 731
Size: 32 4266
PstI C_TGCA'G
Cuts at: 870 1134 1345 2030 870
Size: 264 211 685 3138
Fragments arranged by size:
3138 685 264 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 1167 1723 2083 1167
Size: 556 360 3382
Fragments arranged by size:
3382 556 360
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 198 1312 198
Size: 1114 3184
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 1160 2321 2531 1160
Size: 1161 210 2927
Fragments arranged by size:
2927 1161 210
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 206 866 1130 1239 1341 2026 3019 3210
Size: 173 660 264 109 102 685 993 191
Cuts at: 3210 33
Size: 1121
Fragments arranged by size:
1121 993 685 660 264 191 173 109
102
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 1320 1320
Size: 4298
SmlI C'TyrA_G
Cuts at: 379 1453 2368 2860 3122 379
Size: 1074 915 492 262 1555
Fragments arranged by size:
1555 1074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
StuI AGG'CCT
Cuts at: 1237 1237
Size: 4298
StyI C'CwwG_G
Cuts at: 716 1432 1786 716
Size: 716 354 3228
Fragments arranged by size:
3228 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 335 1881 2014 2473 2682 335
Size: 1546 133 459 209 1951
Fragments arranged by size:
1951 1546 459 209 133
TatI w'GTAC_w
Cuts at: 495 3813 495
Size: 3318 980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 3815 3847 662
Size: 32 3121 32 1113
Fragments arranged by size:
3121 1113 32 32
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 1793 312
Size: 1481 2817
XmaI C'CCGG_G
Cuts at: 1318 1318
Size: 4298
XmnI GAAnn'nnTTC
Cuts at: 762 1154 762
Size: 392 3906
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI