Restriction map for
pF1KB7950
(Circular) (Six-base) MAPSORT of: pF1KB7950.seq Check: 2509 from: 1 to: 4295
pF1KB7950 4295 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 2120 2552 3299 3567 866
Size: 1254 432 747 268 1594
Fragments arranged by size:
1594 1254 747 432 268
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 854 854
Size: 4295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 442
Size: 32 4263
AlwNI CAG_nnn'CTG
Cuts at: 738 1600 3167 738
Size: 862 1567 1866
Fragments arranged by size:
1866 1567 862
ApaLI G'TGCA_C
Cuts at: 399 3065 399
Size: 2666 1629
ApoI r'AATT_y
Cuts at: 292 1299 3648 3904 292
Size: 1007 2349 256 683
Fragments arranged by size:
2349 1007 683 256
AscI GG'CGCG_CC
Cuts at: 545 545
Size: 4295
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 138 364 422 500 551 1315 138
Size: 226 58 78 51 764 3118
Fragments arranged by size:
3118 764 226 78 58 51
BamHI G'GATC_C
Cuts at: 786 1320 786
Size: 534 3761
BanI G'GyrC_C
Cuts at: 267 303 618 1119 1311 1725 1973 2008 267
Size: 36 315 501 192 414 248 35 2554
Fragments arranged by size:
2554 501 414 315 248 192 36 35
BanII G_rGCy'C
Cuts at: 682 1309 2339 682
Size: 627 1030 2638
Fragments arranged by size:
2638 1030 627
BbeI G_GCGC'C
Cuts at: 271 622 1729 1977 271
Size: 351 1107 248 2589
Fragments arranged by size:
2589 1107 351 248
BbvCI CC'TCA_GC
Cuts at: 1108 1108
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 403 1124 1920 2013 3069 403
Size: 721 796 93 1056 1629
Fragments arranged by size:
1629 1056 796 721 93
BmgBI CAC'GTC
Cuts at: 320 320
Size: 4295
BmrI ACTGGGnnnn_n'
Cuts at: 987 1507 1588 1923 4067 987
Size: 520 81 335 2144 1215
Fragments arranged by size:
2144 1215 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 752 2662 752
Size: 1910 2385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 201 532 839 931 1084 148
Size: 53 331 307 92 153 3359
Fragments arranged by size:
3359 331 307 153 92 53
Bpu10I CC'TnA_GC
Cuts at: 1108 1165 1108
Size: 57 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 2842 3140 1471
Size: 879 492 298 2626
Fragments arranged by size:
2626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3999 3999
Size: 4295
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 268 571 619 1726 1974 2676 4172 268
Size: 303 48 1107 248 702 1496 391
Fragments arranged by size:
1496 1107 702 391 303 248 48
BsaWI w'CCGG_w
Cuts at: 403 1484 2005 2683 2957 3104 3515 403
Size: 1081 521 678 274 147 411 1183
Fragments arranged by size:
1183 1081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 442
Size: 30 4265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 958 958
Size: 4295
BseYI C'CCAG_C
Cuts at: 271 440 595 639 1720 2478 3055 3551 271
Size: 169 155 44 1081 758 577 496 1015
Fragments arranged by size:
1081 1015 758 577 496 169 155 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 573
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 531 1837 1883 3091 104
Size: 427 1306 46 1208 1308
Fragments arranged by size:
1308 1306 1208 427 46
BsiHKAI G_wGCw'C
Cuts at: 403 1309 2087 2277 3069 403
Size: 906 778 190 792 1629
Fragments arranged by size:
1629 906 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 401 401
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 403 682 1124 1309 1920 2013 2087 2277 2339
Size: 279 442 185 611 93 74 190 62
Cuts at: 2339 3069 403
Size: 730 1629
Fragments arranged by size:
1629 730 611 442 279 190 185 93
74 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1094 1345 1559 1861 2242 1094
Size: 251 214 302 381 3147
Fragments arranged by size:
3147 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 162 193 260 488 2587 2641 4125 4290
Size: 112 31 67 228 2099 54 1484 165
Cuts at: 4290 50
Size: 55
Fragments arranged by size:
2099 1484 228 165 112 67 55 54
31
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 143 154 530 607 753 1244 2293 2474 2683
Size: 11 376 77 146 491 1049 181 209
Cuts at: 2683 143
Size: 1755
Fragments arranged by size:
1755 1049 491 376 209 181 146 77
11
BssHII G'CGCG_C
Cuts at: 545 545
Size: 4295
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 1257 1672 1257
Size: 415 3880
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstEII G'GTnAC_C
Cuts at: 756 756
Size: 4295
BstYI r'GATC_y
Cuts at: 1 786 1320 1787 1810 2145 2391 3403 3489
Size: 785 534 467 23 335 246 1012 86
Cuts at: 3489 3501 3847 1
Size: 12 346 449
Fragments arranged by size:
1012 785 534 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 263 385 263
Size: 122 4173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 293 2245 2426 2589 293
Size: 1952 181 163 1999
Fragments arranged by size:
1999 1952 181 163
BtsI GCAGTG_nn'
Cuts at: 1538 1538
Size: 4295
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 1130 1880 2054 2445 2472 3817 1130
Size: 750 174 391 27 1345 1608
Fragments arranged by size:
1608 1345 750 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 721 869 896 947 1204 2318 2528 721
Size: 148 27 51 257 1114 210 2488
Fragments arranged by size:
2488 1114 257 210 148 51 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 564 1092 2503 2813 2959 4105 564
Size: 528 1411 310 146 1146 754
Fragments arranged by size:
1411 1146 754 528 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 201 532 839 866 931 1084 2120 2552
Size: 53 331 307 27 65 153 1036 432
Cuts at: 2552 3299 3567 148
Size: 747 268 876
Fragments arranged by size:
1036 876 747 432 331 307 268 153
65 53 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1168 1168
Size: 4295
EcoO109I rG'GnC_Cy
Cuts at: 340 354 892 1146 1434 340
Size: 14 538 254 288 3201
Fragments arranged by size:
3201 538 288 254 14
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 271 622 1108 1729 1977 2999 3978 271
Size: 351 486 621 248 1022 979 588
Fragments arranged by size:
1022 979 621 588 486 351 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 948 980 1035 1067 2137 2167 2169
Size: 32 723 32 55 32 1070 30 2
Cuts at: 2169 2199 193
Size: 30 2289
Fragments arranged by size:
2289 1070 723 55 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 267 618 1725 1973 267
Size: 351 1107 248 2589
Fragments arranged by size:
2589 1107 351 248
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 1132 2056 1132
Size: 924 3371
MslI CAynn'nnrTG
Cuts at: 112 766 836 2284 2411 112
Size: 654 70 1448 127 1996
Fragments arranged by size:
1996 1448 654 127 70
MspA1I CmG'CkG
Cuts at: 639 1136 1406 1720 2080 3093 3338 639
Size: 497 270 314 360 1013 245 1596
Fragments arranged by size:
1596 1013 497 360 314 270 245
NaeI GCC'GGC
Cuts at: 145 609 1246 2476 145
Size: 464 637 1230 1964
Fragments arranged by size:
1964 1230 637 464
NarI GG'CG_CC
Cuts at: 268 619 1726 1974 268
Size: 351 1107 248 2589
Fragments arranged by size:
2589 1107 351 248
NgoMIV G'CCGG_C
Cuts at: 143 607 1244 2474 143
Size: 464 637 1230 1964
Fragments arranged by size:
1964 1230 637 464
NruI TCG'CGA
Cuts at: 1095 1095
Size: 4295
NspI r_CATG'y
Cuts at: 483 1348 1848 2755 483
Size: 865 500 907 2023
Fragments arranged by size:
2023 907 865 500
PasI CC'CwG_GG
Cuts at: 329 329
Size: 4295
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PfoI T'CCnGG_A
Cuts at: 1061 1061
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 474 3434 3466 442
Size: 32 2960 32 1271
Fragments arranged by size:
2960 1271 32 32
PpuMI rG'GwC_Cy
Cuts at: 340 354 892 340
Size: 14 538 3743
Fragments arranged by size:
3743 538 14
PstI C_TGCA'G
Cuts at: 927 1342 2027 927
Size: 415 685 3195
Fragments arranged by size:
3195 685 415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1136 1720 2080 1136
Size: 584 360 3351
Fragments arranged by size:
3351 584 360
RsrII CG'GwC_CG
Cuts at: 157 2490 157
Size: 2333 1962
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SanDI GG'GwC_CC
Cuts at: 340 354 892 340
Size: 14 538 3743
Fragments arranged by size:
3743 538 14
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SexAI A'CCwGG_T
Cuts at: 1115 1115
Size: 4295
SfcI C'TryA_G
Cuts at: 33 923 974 1220 1338 2023 3016 3207 33
Size: 890 51 246 118 685 993 191 1121
Fragments arranged by size:
1121 993 890 685 246 191 118 51
SfoI GGC'GCC
Cuts at: 269 620 1727 1975 269
Size: 351 1107 248 2589
Fragments arranged by size:
2589 1107 351 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 366 424 502 553 1317 366
Size: 58 78 51 764 3344
Fragments arranged by size:
3344 764 78 58 51
SmlI C'TyrA_G
Cuts at: 1450 2365 2857 3119 1450
Size: 915 492 262 2626
Fragments arranged by size:
2626 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 661 1174 661
Size: 513 3782
StyI C'CwwG_G
Cuts at: 566 1142 1175 1429 1783 566
Size: 576 33 254 354 3078
Fragments arranged by size:
3078 576 354 254 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1878 2011 2470 2679 1878
Size: 133 459 209 3494
Fragments arranged by size:
3494 459 209 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1790 1790
Size: 4295
XmaI C'CCGG_G
Cuts at: 364 422 500 551 1315 364
Size: 58 78 51 764 3344
Fragments arranged by size:
3344 764 78 58 51
XmnI GAAnn'nnTTC
Cuts at: 292 802 905 292
Size: 510 103 3682
Fragments arranged by size:
3682 510 103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AvrII
BaeI BbsI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI