Restriction map for
pF1KB7947
(Circular) (Six-base) MAPSORT of: pF1KB7947.seq Check: 8217 from: 1 to: 3875
pF1KB7947 3875 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2879 3147 1700
Size: 432 747 268 2428
Fragments arranged by size:
2428 747 432 268
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 128 157 1180 2747 128
Size: 29 1023 1567 1256
Fragments arranged by size:
1567 1256 1023 29
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 879 3228 3484 879
Size: 2349 256 1270
Fragments arranged by size:
2349 1270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 111 900 111
Size: 789 3086
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2534 3875 1773
Size: 761 1341 1773
Fragments arranged by size:
1773 1341 761
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 685 127
Size: 558 3317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2422 2720 1051
Size: 879 492 298 2206
Fragments arranged by size:
2206 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 407 1306 1554 2256 3752 407
Size: 899 248 702 1496 530
Fragments arranged by size:
1496 899 702 530 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 840 870 658
Size: 30 152 30 3663
Fragments arranged by size:
3663 152 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 352 150
Size: 202 3673
BseYI C'CCAG_C
Cuts at: 150 1300 2058 2635 3131 150
Size: 1150 758 577 496 894
Fragments arranged by size:
1150 894 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 344 526 889 1667 1857 2649 344
Size: 182 363 778 190 792 1570
Fragments arranged by size:
1570 792 778 363 190 182
Bsp1286I G_dGCh'C
Cuts at: 344 526 889 1500 1593 1667 1857 1919 2649
Size: 182 363 611 93 74 190 62 730
Cuts at: 2649 344
Size: 1570
Fragments arranged by size:
1570 730 611 363 190 182 93 74
62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 1787 201
Size: 1586 2289
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 676 1252 676
Size: 576 3299
BstBI TT'CG_AA
Cuts at: 174 2236 174
Size: 2062 1813
BstXI CCAn_nnnn'nTGG
Cuts at: 453 468 453
Size: 15 3860
BstYI r'GATC_y
Cuts at: 1 111 900 1367 1390 1725 1971 2983 3069
Size: 110 789 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 789 467 449 346 335 246 110
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 204 833 204
Size: 629 3246
BtgI C'CryG_G
Cuts at: 107 116 107
Size: 9 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 515 1825 2006 2169 515
Size: 1310 181 163 2221
Fragments arranged by size:
2221 1310 181 163
BtsI GCAGTG_nn'
Cuts at: 524 1118 524
Size: 594 3281
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 119 1460 1634 2025 2052 3397 119
Size: 1341 174 391 27 1345 597
Fragments arranged by size:
1345 1341 597 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 573 2083 2393 2539 3685 573
Size: 1510 310 146 1146 763
Fragments arranged by size:
1510 1146 763 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 685 1700 2132 2879 3147 127
Size: 558 1015 432 747 268 855
Fragments arranged by size:
1015 855 747 558 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 331 331
Size: 3875
EcoO109I rG'GnC_Cy
Cuts at: 200 828 1014 200
Size: 628 186 3061
Fragments arranged by size:
3061 628 186
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 747 1309 1557 2579 3558 747
Size: 562 248 1022 979 1064
Fragments arranged by size:
1064 1022 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 829 861 1717 1747 1749 1779 532
Size: 32 265 32 856 30 2 30 2628
Fragments arranged by size:
2628 856 265 32 32 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 118 128 654 986 1300 1660 2673 2918 118
Size: 10 526 332 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 526 360 332 314 245 10
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 107
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 928 1428 2335 928
Size: 500 907 2468
Fragments arranged by size:
2468 907 500
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PpuMI rG'GwC_Cy
Cuts at: 828 828
Size: 3875
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 128 654 1300 1660 128
Size: 526 646 360 2343
Fragments arranged by size:
2343 646 526 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SacII CC_GC'GG
Cuts at: 119 119
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 918 1603 2596 2787 33
Size: 885 685 993 191 1121
Fragments arranged by size:
1121 993 885 685 191
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2437 2699 1030
Size: 915 492 262 2206
Fragments arranged by size:
2206 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 446 644 1009 1363 107
Size: 339 198 365 354 2619
Fragments arranged by size:
2619 365 354 339 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 840 872 3392 3424 840
Size: 32 2520 32 1291
Fragments arranged by size:
2520 1291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 158 1370 158
Size: 1212 2663
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI