Restriction map for
pF1KB7944
(Circular) (Six-base) MAPSORT of: pF1KB7944.seq Check: 550 from: 1 to: 3851
pF1KB7944 3851 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 347 347
Size: 3851
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 2108 2855 3123 1676
Size: 432 747 268 2404
Fragments arranged by size:
2404 747 432 268
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 324 358 1156 2723 324
Size: 34 798 1567 1452
Fragments arranged by size:
1567 1452 798 34
ApaI G_GGCC'C
Cuts at: 181 181
Size: 3851
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 174 871 174
Size: 697 3154
BamHI G'GATC_C
Cuts at: 226 876 226
Size: 650 3201
BanI G'GyrC_C
Cuts at: 442 463 867 1281 1529 1564 442
Size: 21 404 414 248 35 2729
Fragments arranged by size:
2729 414 404 248 35 21
BanII G_rGCy'C
Cuts at: 162 181 265 833 865 1895 162
Size: 19 84 568 32 1030 2118
Fragments arranged by size:
2118 1030 568 84 32 19
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BbsI GAAGACnn'nnnn_
Cuts at: 596 596
Size: 3851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 321 355 321
Size: 34 3817
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglI GCCn_nnn'nGGC
Cuts at: 137 137
Size: 3851
BglII A'GATC_T
Cuts at: 809 1366 3403 809
Size: 557 2037 1257
Fragments arranged by size:
2037 1257 557
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 181 468 1476 1569 2625 181
Size: 287 1008 93 1056 1407
Fragments arranged by size:
1407 1056 1008 287 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 279 2218 279
Size: 1939 1912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 289 562 826 232
Size: 57 273 264 3257
Fragments arranged by size:
3257 273 264 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 344 1282 1530 2232 3728 344
Size: 938 248 702 1496 467
Fragments arranged by size:
1496 938 702 467 248
BsaWI w'CCGG_w
Cuts at: 488 1040 1561 2239 2513 2660 3071 488
Size: 552 521 678 274 147 411 1268
Fragments arranged by size:
1268 678 552 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 682
Size: 30 3821
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 286 831 271
Size: 15 545 3291
Fragments arranged by size:
3291 545 15
BseYI C'CCAG_C
Cuts at: 163 180 435 1276 2034 2611 3107 163
Size: 17 255 841 758 577 496 907
Fragments arranged by size:
907 841 758 577 496 255 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 378
Size: 3851
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2625 865
Size: 778 190 792 2091
Fragments arranged by size:
2091 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 162 181 265 468 833 865 1476 1569 1643
Size: 19 84 203 365 32 611 93 74
Cuts at: 1643 1833 1895 2625 162
Size: 190 62 730 1388
Fragments arranged by size:
1388 730 611 365 203 190 93 84
74 62 32 19
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 755 901 1115 1417 1798 755
Size: 146 214 302 381 2808
Fragments arranged by size:
2808 381 302 214 146
BsrBI CCG'CTC
Cuts at: 50 122 2143 2197 3681 3846 50
Size: 72 2021 54 1484 165 55
Fragments arranged by size:
2021 1484 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 306 1763 306
Size: 1457 2394
BsrFI r'CCGG_y
Cuts at: 1849 2030 2239 1849
Size: 181 209 3461
Fragments arranged by size:
3461 209 181
BssSI C'ACGA_G
Cuts at: 696 2122 2480 696
Size: 1426 358 2067
Fragments arranged by size:
2067 1426 358
BstAPI GCAn_nnn'nTGC
Cuts at: 821 1228 821
Size: 407 3444
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstXI CCAn_nnnn'nTGG
Cuts at: 301 301
Size: 3851
BstYI r'GATC_y
Cuts at: 1 226 569 809 876 1343 1366 1701 1947
Size: 225 343 240 67 467 23 335 246
Cuts at: 1947 2959 3045 3057 3403 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 343 335 246 240
225 86 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 374 374
Size: 3851
BtgI C'CryG_G
Cuts at: 794 794
Size: 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 603 1094 603
Size: 491 3360
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 343 598 1557 2415 343
Size: 255 959 858 1779
Fragments arranged by size:
1779 959 858 255
EaeI y'GGCC_r
Cuts at: 123 420 1436 1610 2001 2028 3373 123
Size: 297 1016 174 391 27 1345 601
Fragments arranged by size:
1345 1016 601 391 297 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 529 1874 2084 529
Size: 1345 210 2296
Fragments arranged by size:
2296 1345 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2059 2369 2515 3661 2059
Size: 310 146 1146 2249
Fragments arranged by size:
2249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 289 562 826 1676 2108 2855 3123 232
Size: 57 273 264 850 432 747 268 960
Fragments arranged by size:
960 850 747 432 273 268 264 57
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 3851
EcoO109I rG'GnC_Cy
Cuts at: 321 990 321
Size: 669 3182
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 132 1285 1533 2555 3534 132
Size: 1153 248 1022 979 449
Fragments arranged by size:
1153 1022 979 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1723 1725 1755 1693
Size: 30 2 30 3789
Fragments arranged by size:
3789 30 30 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2522 2706 2522
Size: 184 3667
MscI TGG'CCA
Cuts at: 422 1612 422
Size: 1190 2661
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 796 962 1276 1636 2649 2894 796
Size: 166 314 360 1013 245 1753
Fragments arranged by size:
1753 1013 360 314 245 166
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 368 904 1404 2311 368
Size: 536 500 907 1908
Fragments arranged by size:
1908 907 536 500
PasI CC'CwG_GG
Cuts at: 318 468 318
Size: 150 3701
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PflMI CCAn_nnn'nTGG
Cuts at: 324 324
Size: 3851
PfoI T'CCnGG_A
Cuts at: 656 773 656
Size: 117 3734
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PspOMI G'GGCC_C
Cuts at: 177 177
Size: 3851
PstI C_TGCA'G
Cuts at: 685 898 1583 685
Size: 213 685 2953
Fragments arranged by size:
2953 685 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SacII CC_GC'GG
Cuts at: 797 797
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SexAI A'CCwGG_T
Cuts at: 113 113
Size: 3851
SfcI C'TryA_G
Cuts at: 33 681 894 1579 2572 2763 33
Size: 648 213 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 213 191
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 176 873 176
Size: 697 3154
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StyI C'CwwG_G
Cuts at: 294 386 479 985 1339 294
Size: 92 93 506 354 2806
Fragments arranged by size:
2806 506 354 93 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 521 1434 1567 2026 2235 460
Size: 61 913 133 459 209 2076
Fragments arranged by size:
2076 913 459 209 133 61
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 3368 3400 682
Size: 32 2654 32 1133
Fragments arranged by size:
2654 1133 32 32
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 133 1346 133
Size: 1213 2638
XmaI C'CCGG_G
Cuts at: 174 871 174
Size: 697 3154
ZraI GAC'GTC
Cuts at: 345 345
Size: 3851
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI