Restriction map for
pF1KB7942
(Circular) (Six-base) MAPSORT of: pF1KB7942.seq Check: 5699 from: 1 to: 3848
pF1KB7942 3848 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 230 513 230
Size: 283 3565
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 842 3688 842
Size: 2846 1002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1673 2105 2852 3120 814
Size: 859 432 747 268 1542
Fragments arranged by size:
1542 859 747 432 268
AflIII A'CryG_T
Cuts at: 730 1200 2304 3281 730
Size: 470 1104 977 1297
Fragments arranged by size:
1297 1104 977 470
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 439 439
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 171 868 171
Size: 697 3151
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 828 864 1278 1526 1561 828
Size: 36 414 248 35 3115
Fragments arranged by size:
3115 414 248 36 35
BanII G_rGCy'C
Cuts at: 862 1892 862
Size: 1030 2818
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 488 488
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 128 1473 1566 2622 128
Size: 1345 93 1056 1354
Fragments arranged by size:
1354 1345 1056 93
BmgBI CAC'GTC
Cuts at: 621 621
Size: 3848
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BmtI G_CTAG'C
Cuts at: 580 580
Size: 3848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 154 253 322 99
Size: 55 99 69 3625
Fragments arranged by size:
3625 99 69 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 657 3552 657
Size: 2895 953
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 681 711 681
Size: 30 3818
BseYI C'CCAG_C
Cuts at: 150 1273 2031 2608 3104 150
Size: 1123 758 577 496 894
Fragments arranged by size:
1123 894 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 734 541
Size: 193 3655
BsiEI CG_ry'CG
Cuts at: 104 159 1390 1436 2644 104
Size: 55 1231 46 1208 1308
Fragments arranged by size:
1308 1231 1208 55 46
BsiHKAI G_wGCw'C
Cuts at: 862 1640 1830 2622 862
Size: 778 190 792 2088
Fragments arranged by size:
2088 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 611 701 611
Size: 90 3758
Bsp1286I G_dGCh'C
Cuts at: 128 862 1473 1566 1640 1830 1892 2622 128
Size: 734 611 93 74 190 62 730 1354
Fragments arranged by size:
1354 734 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 513 898 1112 1414 1795 230
Size: 283 385 214 302 381 2283
Fragments arranged by size:
2283 385 381 302 283 214
BsrBI CCG'CTC
Cuts at: 50 138 450 2140 2194 3678 3843 50
Size: 88 312 1690 54 1484 165 55
Fragments arranged by size:
1690 1484 312 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 776 1760 776
Size: 984 2864
BsrFI r'CCGG_y
Cuts at: 144 1846 2027 2236 144
Size: 1702 181 209 1756
Fragments arranged by size:
1756 1702 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstYI r'GATC_y
Cuts at: 1 873 1340 1363 1698 1944 2956 3042 3054
Size: 872 467 23 335 246 1012 86 12
Cuts at: 3054 3400 1
Size: 346 449
Fragments arranged by size:
1012 872 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 710 710
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 156 288 767 1433 1607 1998 2025 3370 156
Size: 132 479 666 174 391 27 1345 634
Fragments arranged by size:
1345 666 634 479 391 174 132 27
EagI C'GGCC_G
Cuts at: 156 1433 156
Size: 1277 2571
EarI CTCTTCn'nnn_
Cuts at: 373 537 1871 2081 373
Size: 164 1334 210 2140
Fragments arranged by size:
2140 1334 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 280 2056 2366 2512 3658 280
Size: 1776 310 146 1146 470
Fragments arranged by size:
1776 1146 470 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 154 253 322 814 1673 2105 2852 3120
Size: 55 99 69 492 859 432 747 268
Cuts at: 3120 99
Size: 827
Fragments arranged by size:
859 827 747 492 432 268 99 69
55
EcoNI CCTnn'n_nnAGG
Cuts at: 508 508
Size: 3848
EcoO109I rG'GnC_Cy
Cuts at: 987 987
Size: 3848
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 151 710 1282 1530 2552 3531 151
Size: 559 572 248 1022 979 468
Fragments arranged by size:
1022 979 572 559 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 664 681 696 713 1690 1720 1722
Size: 32 188 17 15 17 977 30 2
Cuts at: 1722 1752 444
Size: 30 2540
Fragments arranged by size:
2540 977 188 32 30 30 17 17
15 2
HincII GTy'rAC
Cuts at: 840 887 840
Size: 47 3801
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 769 1609 769
Size: 840 3008
MslI CAynn'nnrTG
Cuts at: 763 1837 1964 763
Size: 1074 127 2647
Fragments arranged by size:
2647 1074 127
MspA1I CmG'CkG
Cuts at: 323 357 423 959 1273 1633 2646 2891 323
Size: 34 66 536 314 360 1013 245 1280
Fragments arranged by size:
1280 1013 536 360 314 245 66 34
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 710 710
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NheI G'CTAG_C
Cuts at: 576 576
Size: 3848
NotI GC'GGCC_GC
Cuts at: 156 156
Size: 3848
NspI r_CATG'y
Cuts at: 734 901 1401 2308 734
Size: 167 500 907 2274
Fragments arranged by size:
2274 907 500 167
PciI A'CATG_T
Cuts at: 730 2304 730
Size: 1574 2274
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PstI C_TGCA'G
Cuts at: 194 428 895 1580 194
Size: 234 467 685 2462
Fragments arranged by size:
2462 685 467 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 357 423 1273 1633 357
Size: 66 850 360 2572
Fragments arranged by size:
2572 850 360 66
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 537 1871 2081 537
Size: 1334 210 2304
Fragments arranged by size:
2304 1334 210
SbfI CC_TGCA'GG
Cuts at: 194 895 194
Size: 701 3147
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SexAI A'CCwGG_T
Cuts at: 824 824
Size: 3848
SfcI C'TryA_G
Cuts at: 33 190 424 891 1576 2569 2760 33
Size: 157 234 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 467 234 191 157
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 173 870 173
Size: 697 3151
SmlI C'TyrA_G
Cuts at: 1003 1918 2410 2672 1003
Size: 915 492 262 2179
Fragments arranged by size:
2179 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
SrfI GCCC'GGGC
Cuts at: 173 173
Size: 3848
StyI C'CwwG_G
Cuts at: 710 800 982 1336 710
Size: 90 182 354 3222
Fragments arranged by size:
3222 354 182 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 693 1645 693
Size: 952 2896
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 171 868 171
Size: 697 3151
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI Bpu10I BseRI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI