Restriction map for
pF1KB7940
(Circular) (Six-base) MAPSORT of: pF1KB7940.seq Check: 1486 from: 1 to: 3833
pF1KB7940 3833 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 460 1658 2090 2837 3105 154
Size: 306 1198 432 747 268 882
Fragments arranged by size:
1198 882 747 432 306 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlwNI CAG_nnn'CTG
Cuts at: 1138 2705 1138
Size: 1567 2266
ApaLI G'TGCA_C
Cuts at: 619 2603 619
Size: 1984 1849
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 527 853 527
Size: 326 3507
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 849 1263 1511 1546 849
Size: 414 248 35 3136
Fragments arranged by size:
3136 414 248 35
BanII G_rGCy'C
Cuts at: 847 1877 847
Size: 1030 2803
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BbsI GAAGACnn'nnnn_
Cuts at: 274 563 274
Size: 289 3544
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglII A'GATC_T
Cuts at: 435 1348 3385 435
Size: 913 2037 883
Fragments arranged by size:
2037 913 883
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 623 1458 1551 2607 623
Size: 835 93 1056 1849
Fragments arranged by size:
1849 1056 835 93
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 2200 273
Size: 1927 1906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 263
Size: 3833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 693 3537 693
Size: 2844 989
BsaAI yAC'GTr
Cuts at: 668 1186 668
Size: 518 3315
BsaBI GATnn'nnATC
Cuts at: 6 235 1347 3384 6
Size: 229 1112 2037 455
Fragments arranged by size:
2037 1112 455 229
BsaHI Gr'CG_yC
Cuts at: 1264 1512 2214 3710 1264
Size: 248 702 1496 1387
Fragments arranged by size:
1496 1387 702 248
BsaWI w'CCGG_w
Cuts at: 475 1022 1543 2221 2495 2642 3053 475
Size: 547 521 678 274 147 411 1255
Fragments arranged by size:
1255 678 547 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 261 291 673 703 261
Size: 30 382 30 3391
Fragments arranged by size:
3391 382 30 30
BseYI C'CCAG_C
Cuts at: 1258 2016 2593 3089 1258
Size: 758 577 496 2002
Fragments arranged by size:
2002 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 3833
BsiEI CG_ry'CG
Cuts at: 104 116 1375 1421 2629 104
Size: 12 1259 46 1208 1308
Fragments arranged by size:
1308 1259 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 134 623 847 1625 1815 2607 134
Size: 489 224 778 190 792 1360
Fragments arranged by size:
1360 792 778 489 224 190
Bsp1286I G_dGCh'C
Cuts at: 134 623 847 1458 1551 1625 1815 1877 2607
Size: 489 224 611 93 74 190 62 730
Cuts at: 2607 134
Size: 1360
Fragments arranged by size:
1360 730 611 489 224 190 93 74
62
BspEI T'CCGG_A
Cuts at: 475 3053 475
Size: 2578 1255
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 883 1097 1399 1780 883
Size: 214 302 381 2936
Fragments arranged by size:
2936 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2125 2179 3663 3828 50
Size: 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1745 1745
Size: 3833
BsrFI r'CCGG_y
Cuts at: 1831 2012 2221 1831
Size: 181 209 3443
Fragments arranged by size:
3443 209 181
BssHII G'CGCG_C
Cuts at: 421 421
Size: 3833
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 399 2194 399
Size: 1795 2038
BstXI CCAn_nnnn'nTGG
Cuts at: 126 737 126
Size: 611 3222
BstYI r'GATC_y
Cuts at: 1 308 435 858 1325 1348 1683 1929 2941
Size: 307 127 423 467 23 335 246 1012
Cuts at: 2941 3027 3039 3385 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 307 246
127 86 23 12
BtgI C'CryG_G
Cuts at: 107 260 647 796 107
Size: 153 387 149 3144
Fragments arranged by size:
3144 387 153 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 465 1076 465
Size: 611 3222
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DraIII CAC_nnn'GTG
Cuts at: 802 802
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 362 1418 1592 1983 2010 3355 362
Size: 1056 174 391 27 1345 840
Fragments arranged by size:
1345 1056 840 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 263 460 1658 2090 2837 3105 154
Size: 109 197 1198 432 747 268 882
Fragments arranged by size:
1198 882 747 432 268 197 109
EcoO109I rG'GnC_Cy
Cuts at: 524 972 524
Size: 448 3385
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 300 1267 1515 2537 3516 300
Size: 967 248 1022 979 617
Fragments arranged by size:
1022 979 967 617 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 1675 1705 1707 1737 261
Size: 32 1382 30 2 30 2357
Fragments arranged by size:
2357 1382 32 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MfeI C'AATT_G
Cuts at: 731 731
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 364 1594 364
Size: 1230 2603
MslI CAynn'nnrTG
Cuts at: 646 1822 1949 646
Size: 1176 127 2530
Fragments arranged by size:
2530 1176 127
MspA1I CmG'CkG
Cuts at: 186 360 944 1258 1618 2631 2876 186
Size: 174 584 314 360 1013 245 1143
Fragments arranged by size:
1143 1013 584 360 314 245 174
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NcoI C'CATG_G
Cuts at: 107 107
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 645 886 1386 2293 645
Size: 241 500 907 2185
Fragments arranged by size:
2185 907 500 241
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 291 291
Size: 3833
PfoI T'CCnGG_A
Cuts at: 806 806
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PpuMI rG'GwC_Cy
Cuts at: 524 524
Size: 3833
PstI C_TGCA'G
Cuts at: 249 305 880 1565 249
Size: 56 575 685 2517
Fragments arranged by size:
2517 685 575 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 186 360 1258 1618 186
Size: 174 898 360 2401
Fragments arranged by size:
2401 898 360 174
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SanDI GG'GwC_CC
Cuts at: 524 524
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SexAI A'CCwGG_T
Cuts at: 683 683
Size: 3833
SfcI C'TryA_G
Cuts at: 33 245 301 321 876 1561 2554 2745 33
Size: 212 56 20 555 685 993 191 1121
Fragments arranged by size:
1121 993 685 555 212 191 56 20
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 529 855 529
Size: 326 3507
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StyI C'CwwG_G
Cuts at: 107 967 1321 107
Size: 860 354 2619
Fragments arranged by size:
2619 860 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1549 2008 2217 1416
Size: 133 459 209 3032
Fragments arranged by size:
3032 459 209 133
TatI w'GTAC_w
Cuts at: 122 251 3348 122
Size: 129 3097 607
Fragments arranged by size:
3097 607 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 673 705 788 820 3350 3382 673
Size: 32 83 32 2530 32 1124
Fragments arranged by size:
2530 1124 83 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 273 1630 273
Size: 1357 2476
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 691 1328 691
Size: 637 3196
XmaI C'CCGG_G
Cuts at: 527 853 527
Size: 326 3507
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI