Restriction map for
pF1KB7939
(Circular) (Six-base) MAPSORT of: pF1KB7939.seq Check: 8939 from: 1 to: 4139
pF1KB7939 4139 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 903 903
Size: 4139
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 241 377 1964 2396 3143 3411 176
Size: 65 136 1587 432 747 268 904
Fragments arranged by size:
1587 904 747 432 268 136 65
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 199 842 1444 3011 199
Size: 643 602 1567 1327
Fragments arranged by size:
1567 1327 643 602
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 250 770 1143 3492 3748 250
Size: 520 373 2349 256 641
Fragments arranged by size:
2349 641 520 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 380 1159 380
Size: 779 3360
BamHI G'GATC_C
Cuts at: 231 820 1164 231
Size: 589 344 3206
Fragments arranged by size:
3206 589 344
BanI G'GyrC_C
Cuts at: 794 1155 1569 1817 1852 794
Size: 361 414 248 35 3081
Fragments arranged by size:
3081 414 361 248 35
BanII G_rGCy'C
Cuts at: 640 1153 2183 640
Size: 513 1030 2596
Fragments arranged by size:
2596 1030 513
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 118 182 365 608 118
Size: 64 183 243 3649
Fragments arranged by size:
3649 243 183 64
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglII A'GATC_T
Cuts at: 395 856 1654 3691 395
Size: 461 798 2037 843
Fragments arranged by size:
2037 843 798 461
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 1764 1857 2913 1764
Size: 93 1056 2990
Fragments arranged by size:
2990 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1107 1107
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 1315 2194 2686 2984 734
Size: 581 879 492 298 1889
Fragments arranged by size:
1889 879 581 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 837 898 1089 837
Size: 61 191 3887
Fragments arranged by size:
3887 191 61
BseYI C'CCAG_C
Cuts at: 1564 2322 2899 3395 1564
Size: 758 577 496 2308
Fragments arranged by size:
2308 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1153 1931 2121 2913 1153
Size: 778 190 792 2379
Fragments arranged by size:
2379 792 778 190
BsmI GAATG_Cn'
Cuts at: 139 139
Size: 4139
Bsp1286I G_dGCh'C
Cuts at: 640 1153 1764 1857 1931 2121 2183 2913 640
Size: 513 611 93 74 190 62 730 1866
Fragments arranged by size:
1866 730 611 513 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 903 1189 1403 1705 2086 903
Size: 286 214 302 381 2956
Fragments arranged by size:
2956 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BssSI C'ACGA_G
Cuts at: 1064 2410 2768 1064
Size: 1346 358 2435
Fragments arranged by size:
2435 1346 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 171 171
Size: 4139
BstYI r'GATC_y
Cuts at: 1 231 395 820 856 1164 1631 1654 1989
Size: 230 164 425 36 308 467 23 335
Cuts at: 1989 2235 3247 3333 3345 3691 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 425 346 335 308 246
230 164 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 965 965
Size: 4139
BtgI C'CryG_G
Cuts at: 342 825 342
Size: 483 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 523 2089 2270 2433 523
Size: 1566 181 163 2229
Fragments arranged by size:
2229 1566 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 1027 1724 1898 2289 2316 3661 1027
Size: 697 174 391 27 1345 1505
Fragments arranged by size:
1505 1345 697 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 992 2162 2372 992
Size: 1170 210 2759
Fragments arranged by size:
2759 1170 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 241 377 1107 1964 2396 3143 3411 176
Size: 65 136 730 857 432 747 268 904
Fragments arranged by size:
904 857 747 730 432 268 136 65
EcoO109I rG'GnC_Cy
Cuts at: 196 1278 196
Size: 1082 3057
EcoRI G'AATT_C
Cuts at: 770 1143 770
Size: 373 3766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 726 2158 417
Size: 309 1432 2398
Fragments arranged by size:
2398 1432 309
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 413 445 505 537 832 864 1981 2011 2013
Size: 32 60 32 295 32 1117 30 2
Cuts at: 2013 2043 413
Size: 30 2509
Fragments arranged by size:
2509 1117 295 60 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 582 2810 2994 353
Size: 229 2228 184 1498
Fragments arranged by size:
2228 1498 229 184
MscI TGG'CCA
Cuts at: 1029 1900 1029
Size: 871 3268
MslI CAynn'nnrTG
Cuts at: 566 2128 2255 566
Size: 1562 127 2450
Fragments arranged by size:
2450 1562 127
MspA1I CmG'CkG
Cuts at: 1118 1250 1564 1924 2937 3182 1118
Size: 132 314 360 1013 245 2075
Fragments arranged by size:
2075 1013 360 314 245 132
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 342 342
Size: 4139
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 571 818 1192 1692 2599 571
Size: 247 374 500 907 2111
Fragments arranged by size:
2111 907 500 374 247
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PfoI T'CCnGG_A
Cuts at: 1084 1084
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PstI C_TGCA'G
Cuts at: 903 949 982 1186 1871 903
Size: 46 33 204 685 3171
Fragments arranged by size:
3171 685 204 46 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 1118 1564 1924 1118
Size: 446 360 3333
Fragments arranged by size:
3333 446 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 899 945 978 1182 1867 2860 3051 33
Size: 866 46 33 204 685 993 191 1121
Fragments arranged by size:
1121 993 866 685 204 191 46 33
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 382 1161 382
Size: 779 3360
SmlI C'TyrA_G
Cuts at: 749 1294 2209 2701 2963 749
Size: 545 915 492 262 1925
Fragments arranged by size:
1925 915 545 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
SrfI GCCC'GGGC
Cuts at: 382 382
Size: 4139
StyI C'CwwG_G
Cuts at: 342 992 1094 1130 1273 1627 342
Size: 650 102 36 143 354 2854
Fragments arranged by size:
2854 650 354 143 102 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1722 1855 2314 2523 1722
Size: 133 459 209 3338
Fragments arranged by size:
3338 459 209 133
TatI w'GTAC_w
Cuts at: 515 3654 515
Size: 3139 1000
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 3656 3688 539
Size: 32 3085 32 990
Fragments arranged by size:
3085 990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 472 788 1634 472
Size: 316 846 2977
Fragments arranged by size:
2977 846 316
XmaI C'CCGG_G
Cuts at: 380 1159 380
Size: 779 3360
XmnI GAAnn'nnTTC
Cuts at: 254 254
Size: 4139
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI ZraI