Restriction map for
pF1KB7936
(Circular) (Six-base) MAPSORT of: pF1KB7936.seq Check: 7993 from: 1 to: 3761
pF1KB7936 3761 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 638 638
Size: 3761
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1586 2018 2765 3033 1586
Size: 432 747 268 2314
Fragments arranged by size:
2314 747 432 268
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 356 356
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 688 765 3114 3370 688
Size: 77 2349 256 1079
Fragments arranged by size:
2349 1079 256 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 214 232 781 214
Size: 18 549 3194
Fragments arranged by size:
3194 549 18
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 124 777 1191 1439 1474 124
Size: 653 414 248 35 2411
Fragments arranged by size:
2411 653 414 248 35
BanII G_rGCy'C
Cuts at: 775 1805 775
Size: 1030 2731
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BbsI GAAGACnn'nnnn_
Cuts at: 326 630 326
Size: 304 3457
BciVI GTATCCnnnnn_n'
Cuts at: 520 1659 2420 3761 520
Size: 1139 761 1341 520
Fragments arranged by size:
1341 1139 761 520
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3761
BglII A'GATC_T
Cuts at: 335 1276 3313 335
Size: 941 2037 783
Fragments arranged by size:
2037 941 783
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 127 301 1386 1479 2535 127
Size: 174 1085 93 1056 1353
Fragments arranged by size:
1353 1085 1056 174 93
BmrI ACTGGGnnnn_n'
Cuts at: 296 973 1054 1389 3533 296
Size: 677 81 335 2144 524
Fragments arranged by size:
2144 677 524 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 244 718 208
Size: 36 474 3251
Fragments arranged by size:
3251 474 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 734 3465 734
Size: 2731 1030
BsaAI yAC'GTr
Cuts at: 374 1114 374
Size: 740 3021
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 635 1192 1440 2142 3638 635
Size: 557 248 702 1496 758
Fragments arranged by size:
1496 758 702 557 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 556
Size: 3761
BseYI C'CCAG_C
Cuts at: 1186 1944 2521 3017 1186
Size: 758 577 496 1930
Fragments arranged by size:
1930 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 605 775 1553 1743 2535 605
Size: 170 778 190 792 1831
Fragments arranged by size:
1831 792 778 190 170
Bsp1286I G_dGCh'C
Cuts at: 127 301 605 775 1386 1479 1553 1743 1805
Size: 174 304 170 611 93 74 190 62
Cuts at: 1805 2535 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 304 190 174 170 93
74 62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 722 2937 722
Size: 2215 1546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 258 811 1025 1327 1708 258
Size: 553 214 302 381 2311
Fragments arranged by size:
2311 553 381 302 214
BsrBI CCG'CTC
Cuts at: 50 448 2053 2107 3591 3756 50
Size: 398 1605 54 1484 165 55
Fragments arranged by size:
1605 1484 398 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 478 2032 2390 478
Size: 1554 358 1849
Fragments arranged by size:
1849 1554 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstXI CCAn_nnnn'nTGG
Cuts at: 309 309
Size: 3761
BstYI r'GATC_y
Cuts at: 1 335 786 1253 1276 1611 1857 2869 2955
Size: 334 451 467 23 335 246 1012 86
Cuts at: 2955 2967 3313 1
Size: 12 346 449
Fragments arranged by size:
1012 467 451 449 346 335 334 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 197 107
Size: 90 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 1346 1520 1911 1938 3283 1346
Size: 174 391 27 1345 1824
Fragments arranged by size:
1824 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 394 1784 1994 394
Size: 1390 210 2161
Fragments arranged by size:
2161 1390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 244 718 1586 2018 2765 3033 208
Size: 36 474 868 432 747 268 936
Fragments arranged by size:
936 868 747 474 432 268 36
EcoO109I rG'GnC_Cy
Cuts at: 270 900 270
Size: 630 3131
EcoRI G'AATT_C
Cuts at: 765 765
Size: 3761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 458 490 1603 1633 1635 1665 327
Size: 32 99 32 1113 30 2 30 2423
Fragments arranged by size:
2423 1113 99 32 32 30 30 2
HincII GTy'rAC
Cuts at: 315 800 315
Size: 485 3276
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2616 2432
Size: 184 3577
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 362 1750 1877 362
Size: 1388 127 2246
Fragments arranged by size:
2246 1388 127
MspA1I CmG'CkG
Cuts at: 738 872 1186 1546 2559 2804 738
Size: 134 314 360 1013 245 1695
Fragments arranged by size:
1695 1013 360 314 245 134
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NcoI C'CATG_G
Cuts at: 107 197 107
Size: 90 3671
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 631 814 1314 2221 631
Size: 183 500 907 2171
Fragments arranged by size:
2171 907 500 183
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PflMI CCAn_nnn'nTGG
Cuts at: 651 651
Size: 3761
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PpuMI rG'GwC_Cy
Cuts at: 270 270
Size: 3761
PspXI vC'TCGA_Gb
Cuts at: 232 232
Size: 3761
PstI C_TGCA'G
Cuts at: 269 808 1493 269
Size: 539 685 2537
Fragments arranged by size:
2537 685 539
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 738 1186 1546 738
Size: 448 360 2953
Fragments arranged by size:
2953 448 360
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 265 804 1489 2482 2673 33
Size: 232 539 685 993 191 1121
Fragments arranged by size:
1121 993 685 539 232 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 232 916 1831 2323 2585 232
Size: 684 915 492 262 1408
Fragments arranged by size:
1408 915 684 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 107 197 619 895 1249 107
Size: 90 422 276 354 2619
Fragments arranged by size:
2619 422 354 276 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1477 1936 2145 1344
Size: 133 459 209 2960
Fragments arranged by size:
2960 459 209 133
TatI w'GTAC_w
Cuts at: 3276 3276
Size: 3761
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 556 1558 556
Size: 1002 2759
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XhoI C'TCGA_G
Cuts at: 232 232
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
ZraI GAC'GTC
Cuts at: 636 636
Size: 3761
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI