Restriction map for
pF1KB7935
(Circular) (Six-base) MAPSORT of: pF1KB7935.seq Check: 8889 from: 1 to: 4226
pF1KB7935 4226 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 695 695
Size: 4226
AccI GT'mk_AC
Cuts at: 1160 1264 1160
Size: 104 4122
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 1220 4066 1220
Size: 2846 1380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 2051 2483 3230 3498 135
Size: 1916 432 747 268 863
Fragments arranged by size:
1916 863 747 432 268
AflIII A'CryG_T
Cuts at: 1578 2682 3659 1578
Size: 1104 977 2145
Fragments arranged by size:
2145 1104 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 324 612 709 1531 3098 324
Size: 288 97 822 1567 1452
Fragments arranged by size:
1567 1452 822 288 97
ApaI G_GGCC'C
Cuts at: 581 581
Size: 4226
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 889 1246 889
Size: 357 3869
BamHI G'GATC_C
Cuts at: 765 822 1251 765
Size: 57 429 3740
Fragments arranged by size:
3740 429 57
BanI G'GyrC_C
Cuts at: 403 922 1242 1656 1904 1939 403
Size: 519 320 414 248 35 2690
Fragments arranged by size:
2690 519 414 320 248 35
BanII G_rGCy'C
Cuts at: 515 581 672 727 971 1240 2270 515
Size: 66 91 55 244 269 1030 2471
Fragments arranged by size:
2471 1030 269 244 91 66 55
BbeI G_GCGC'C
Cuts at: 407 1660 1908 407
Size: 1253 248 2725
Fragments arranged by size:
2725 1253 248
BbsI GAAGACnn'nnnn_
Cuts at: 319 1005 319
Size: 686 3540
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 865 1093 1338 865
Size: 228 245 3753
Fragments arranged by size:
3753 245 228
Bme1580I G_kGCm'C
Cuts at: 426 581 1851 1944 3000 426
Size: 155 1270 93 1056 1652
Fragments arranged by size:
1652 1270 1056 155 93
BmgBI CAC'GTC
Cuts at: 756 756
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 294 888 1438 1519 1854 3998 294
Size: 594 550 81 335 2144 522
Fragments arranged by size:
2144 594 550 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1192 556
Size: 636 3590
Bpu10I CC'TnA_GC
Cuts at: 238 238
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1164 1402 2281 2773 3071 448
Size: 716 238 879 492 298 1603
Fragments arranged by size:
1603 879 716 492 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 479 3930 479
Size: 3451 775
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 404 692 1010 1657 1905 2607 4103 404
Size: 288 318 647 248 702 1496 527
Fragments arranged by size:
1496 702 647 527 318 288 248
BsaWI w'CCGG_w
Cuts at: 742 1415 1936 2614 2888 3035 3446 742
Size: 673 521 678 274 147 411 1522
Fragments arranged by size:
1522 678 673 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 723 445
Size: 278 3948
BseYI C'CCAG_C
Cuts at: 476 790 1651 2409 2986 3482 476
Size: 314 861 758 577 496 1220
Fragments arranged by size:
1220 861 758 577 496 314
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 515 727 971 1240 2018 2208 3000 515
Size: 212 244 269 778 190 792 1741
Fragments arranged by size:
1741 792 778 269 244 212 190
BsmBI CGTCTCn'nnnn_
Cuts at: 699 699
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 426 515 581 672 727 971 1240 1851 1944
Size: 89 66 91 55 244 269 611 93
Cuts at: 1944 2018 2208 2270 3000 426
Size: 74 190 62 730 1652
Fragments arranged by size:
1652 730 611 269 244 190 93 91
89 74 66 62 55
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 983 3402 983
Size: 2419 1807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 1276 1490 1792 2173 309
Size: 967 214 302 381 2362
Fragments arranged by size:
2362 967 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 851 1148 2224 2405 2614 851
Size: 297 1076 181 209 2463
Fragments arranged by size:
2463 1076 297 209 181
BsrGI T'GTAC_A
Cuts at: 161 161
Size: 4226
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 285 285
Size: 4226
BstYI r'GATC_y
Cuts at: 1 765 822 1127 1251 1718 1741 2076 2322
Size: 764 57 305 124 467 23 335 246
Cuts at: 2322 3334 3420 3432 3778 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 764 467 449 346 335 305 246
124 86 57 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 172 809 2176 2357 2520 172
Size: 637 1367 181 163 1878
Fragments arranged by size:
1878 1367 637 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DraIII CAC_nnn'GTG
Cuts at: 391 391
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 1146 1811 1985 2376 2403 3748 1146
Size: 665 174 391 27 1345 1624
Fragments arranged by size:
1624 1345 665 391 174 27
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 507 790 895 1193 2249 2459 507
Size: 283 105 298 1056 210 2274
Fragments arranged by size:
2274 1056 298 283 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 513 725 969 1238 513
Size: 212 244 269 3501
Fragments arranged by size:
3501 269 244 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 556 1192 2051 2483 3230 3498 135
Size: 421 636 859 432 747 268 863
Fragments arranged by size:
863 859 747 636 432 421 268
EcoO109I rG'GnC_Cy
Cuts at: 564 577 578 978 1365 564
Size: 13 1 400 387 3425
Fragments arranged by size:
3425 400 387 13 1
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FseI GG_CCGG'CC
Cuts at: 855 855
Size: 4226
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 374 407 1660 1908 2930 3909 374
Size: 33 1253 248 1022 979 691
Fragments arranged by size:
1253 1022 979 691 248 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2068 2098 2100 2130 2068
Size: 30 2 30 4164
Fragments arranged by size:
4164 30 30 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
KasI G'GCGC_C
Cuts at: 403 1656 1904 403
Size: 1253 248 2725
Fragments arranged by size:
2725 1253 248
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MfeI C'AATT_G
Cuts at: 417 417
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 499 1195 2897 3081 499
Size: 696 1702 184 1644
Fragments arranged by size:
1702 1644 696 184
MscI TGG'CCA
Cuts at: 1987 1987
Size: 4226
MslI CAynn'nnrTG
Cuts at: 2215 2342 2215
Size: 127 4099
MspA1I CmG'CkG
Cuts at: 281 480 656 1337 1651 2011 3024 3269 281
Size: 199 176 681 314 360 1013 245 1238
Fragments arranged by size:
1238 1013 681 360 314 245 199 176
NaeI GCC'GGC
Cuts at: 853 1150 2407 853
Size: 297 1257 2672
Fragments arranged by size:
2672 1257 297
NarI GG'CG_CC
Cuts at: 404 1657 1905 404
Size: 1253 248 2725
Fragments arranged by size:
2725 1253 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 851 1148 2405 851
Size: 297 1257 2672
Fragments arranged by size:
2672 1257 297
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PasI CC'CwG_GG
Cuts at: 574 574
Size: 4226
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PfoI T'CCnGG_A
Cuts at: 557 944 1130 557
Size: 387 186 3653
Fragments arranged by size:
3653 387 186
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PshAI GACnn'nnGTC
Cuts at: 623 623
Size: 4226
PspOMI G'GGCC_C
Cuts at: 577 577
Size: 4226
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 281 480 656 1651 2011 281
Size: 199 176 995 360 2496
Fragments arranged by size:
2496 995 360 199 176
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 515 727 971 1240 515
Size: 212 244 269 3501
Fragments arranged by size:
3501 269 244 212
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 507 790 895 2249 2459 507
Size: 283 105 1354 210 2274
Fragments arranged by size:
2274 1354 283 210 105
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 877 3743 877
Size: 2866 1360
SfcI C'TryA_G
Cuts at: 33 1269 1954 2947 3138 33
Size: 1236 685 993 191 1121
Fragments arranged by size:
1236 1121 993 685 191
SfoI GGC'GCC
Cuts at: 405 1658 1906 405
Size: 1253 248 2725
Fragments arranged by size:
2725 1253 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 427 889 1179 1381 2296 2788 3050 427
Size: 462 290 202 915 492 262 1603
Fragments arranged by size:
1603 915 492 462 290 262 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StyI C'CwwG_G
Cuts at: 107 1360 1714 107
Size: 1253 354 2619
Fragments arranged by size:
2619 1253 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 940 1809 1942 2401 2610 940
Size: 869 133 459 209 2556
Fragments arranged by size:
2556 869 459 209 133
TatI w'GTAC_w
Cuts at: 161 875 3741 161
Size: 714 2866 646
Fragments arranged by size:
2866 714 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 740 772 3743 3775 740
Size: 32 2971 32 1191
Fragments arranged by size:
2971 1191 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XhoI C'TCGA_G
Cuts at: 889 889
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
XmnI GAAnn'nnTTC
Cuts at: 1202 1202
Size: 4226
ZraI GAC'GTC
Cuts at: 693 693
Size: 4226
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI
BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI