Restriction map for
pF1KB7929
(Circular) (Six-base) MAPSORT of: pF1KB7929.seq Check: 2925 from: 1 to: 4169
pF1KB7929 4169 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 444 444
Size: 4169
AatII G_ACGT'C
Cuts at: 486 486
Size: 4169
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 941 1994 2426 3173 3441 626
Size: 315 1053 432 747 268 1354
Fragments arranged by size:
1354 1053 747 432 315 268
AflII C'TTAA_G
Cuts at: 490 490
Size: 4169
AflIII A'CryG_T
Cuts at: 804 1013 1521 2625 3602 804
Size: 209 508 1104 977 1371
Fragments arranged by size:
1371 1104 977 508 209
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 339 919 1474 3041 339
Size: 580 555 1567 1467
Fragments arranged by size:
1567 1467 580 555
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 690 880 1173 3522 3778 690
Size: 190 293 2349 256 1081
Fragments arranged by size:
2349 1081 293 256 190
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 229 858 1189 229
Size: 629 331 3209
Fragments arranged by size:
3209 629 331
BamHI G'GATC_C
Cuts at: 961 1194 961
Size: 233 3936
BanI G'GyrC_C
Cuts at: 163 295 747 1185 1599 1847 1882 163
Size: 132 452 438 414 248 35 2450
Fragments arranged by size:
2450 452 438 414 248 132 35
BanII G_rGCy'C
Cuts at: 1183 2213 1183
Size: 1030 3139
BbeI G_GCGC'C
Cuts at: 167 299 1603 1851 167
Size: 132 1304 248 2485
Fragments arranged by size:
2485 1304 248 132
BbsI GAAGACnn'nnnn_
Cuts at: 114 114
Size: 4169
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 133 577 1281 133
Size: 444 704 3021
Fragments arranged by size:
3021 704 444
Bme1580I G_kGCm'C
Cuts at: 1794 1887 2943 1794
Size: 93 1056 3020
Fragments arranged by size:
3020 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 655 1381 1462 1797 3941 655
Size: 726 81 335 2144 883
Fragments arranged by size:
2144 883 726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 346 1345 2224 2716 3014 244
Size: 102 999 879 492 298 1399
Fragments arranged by size:
1399 999 879 492 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 744 1016 1522 744
Size: 272 506 3391
Fragments arranged by size:
3391 506 272
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 164 296 483 1600 1848 2550 4046 164
Size: 132 187 1117 248 702 1496 287
Fragments arranged by size:
1496 1117 702 287 248 187 132
BsaWI w'CCGG_w
Cuts at: 284 1358 1879 2557 2831 2978 3389 284
Size: 1074 521 678 274 147 411 1064
Fragments arranged by size:
1074 1064 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 521 1594 2352 2929 3425 521
Size: 1073 758 577 496 1265
Fragments arranged by size:
1265 1073 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1174 472
Size: 702 3467
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1183 1961 2151 2943 1183
Size: 778 190 792 2409
Fragments arranged by size:
2409 792 778 190
BsmI GAATG_Cn'
Cuts at: 239 239
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 1183 1794 1887 1961 2151 2213 2943 1183
Size: 611 93 74 190 62 730 2409
Fragments arranged by size:
2409 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 444 1219 1433 1735 2116 444
Size: 775 214 302 381 2497
Fragments arranged by size:
2497 775 381 302 214
BsrBI CCG'CTC
Cuts at: 50 174 180 2461 2515 3999 4164 50
Size: 124 6 2281 54 1484 165 55
Fragments arranged by size:
2281 1484 165 124 55 54 6
BsrDI GCAATG_nn'
Cuts at: 570 2081 570
Size: 1511 2658
BsrFI r'CCGG_y
Cuts at: 188 292 2167 2348 2557 188
Size: 104 1875 181 209 1800
Fragments arranged by size:
1875 1800 209 181 104
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 339 1546 339
Size: 1207 2962
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 862 961 1194 1661 1684 2019 2265 3277
Size: 861 99 233 467 23 335 246 1012
Cuts at: 3277 3363 3375 3721 1
Size: 86 12 346 449
Fragments arranged by size:
1012 861 467 449 346 335 246 233
99 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 373 594 1412 373
Size: 221 818 3130
Fragments arranged by size:
3130 818 221
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 186 812 1754 1928 2319 2346 3691 186
Size: 626 942 174 391 27 1345 664
Fragments arranged by size:
1345 942 664 626 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 941 1994 2426 3173 3441 626
Size: 315 1053 432 747 268 1354
Fragments arranged by size:
1354 1053 747 432 315 268
EcoO109I rG'GnC_Cy
Cuts at: 336 1308 336
Size: 972 3197
EcoRI G'AATT_C
Cuts at: 690 1173 690
Size: 483 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 542 786 2188 438
Size: 104 244 1402 2419
Fragments arranged by size:
2419 1402 244 104
FseI GG_CCGG'CC
Cuts at: 192 192
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 167 299 1603 1851 2873 3852 167
Size: 132 1304 248 1022 979 484
Fragments arranged by size:
1304 1022 979 484 248 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2011 2041 2043 2073 2011
Size: 30 2 30 4107
Fragments arranged by size:
4107 30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 163 295 1599 1847 163
Size: 132 1304 248 2485
Fragments arranged by size:
2485 1304 248 132
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 814 1930 814
Size: 1116 3053
MslI CAynn'nnrTG
Cuts at: 826 2158 2285 826
Size: 1332 127 2710
Fragments arranged by size:
2710 1332 127
MspA1I CmG'CkG
Cuts at: 137 200 1094 1149 1280 1594 1954 2967 3212
Size: 63 894 55 131 314 360 1013 245
Cuts at: 3212 137
Size: 1094
Fragments arranged by size:
1094 1013 894 360 314 245 131 63
55
NaeI GCC'GGC
Cuts at: 190 2350 190
Size: 2160 2009
NarI GG'CG_CC
Cuts at: 164 296 1600 1848 164
Size: 132 1304 248 2485
Fragments arranged by size:
2485 1304 248 132
NgoMIV G'CCGG_C
Cuts at: 188 2348 188
Size: 2160 2009
NspI r_CATG'y
Cuts at: 808 1222 1722 2629 808
Size: 414 500 907 2348
Fragments arranged by size:
2348 907 500 414
PciI A'CATG_T
Cuts at: 804 2625 804
Size: 1821 2348
PfoI T'CCnGG_A
Cuts at: 206 206
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PstI C_TGCA'G
Cuts at: 1082 1216 1901 1082
Size: 134 685 3350
Fragments arranged by size:
3350 685 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 1094 1594 1954 1094
Size: 500 360 3309
Fragments arranged by size:
3309 500 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 195 1078 1212 1897 2890 3081 33
Size: 162 883 134 685 993 191 1121
Fragments arranged by size:
1121 993 883 685 191 162 134
SfoI GGC'GCC
Cuts at: 165 297 1601 1849 165
Size: 132 1304 248 2485
Fragments arranged by size:
2485 1304 248 132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 259 361 490 1324 2239 2731 2993 259
Size: 102 129 834 915 492 262 1435
Fragments arranged by size:
1435 915 834 492 262 129 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 1303 1657 1303
Size: 354 3815
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 684 1752 1885 2344 2553 424
Size: 260 1068 133 459 209 2040
Fragments arranged by size:
2040 1068 459 260 209 133
TatI w'GTAC_w
Cuts at: 3684 3684
Size: 4169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 518 1664 518
Size: 1146 3023
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
ZraI GAC'GTC
Cuts at: 484 484
Size: 4169
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI