Restriction map for
pF1KB7927
(Circular) (Six-base) MAPSORT of: pF1KB7927.seq Check: 1719 from: 1 to: 3713
pF1KB7927 3713 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 707 3553 707
Size: 2846 867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1538 1970 2717 2985 178
Size: 1360 432 747 268 906
Fragments arranged by size:
1360 906 747 432 268
AflIII A'CryG_T
Cuts at: 555 1065 2169 3146 555
Size: 510 1104 977 1122
Fragments arranged by size:
1122 1104 977 510
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 1018 2585 1018
Size: 1567 2146
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 210 717 3066 3322 210
Size: 507 2349 256 601
Fragments arranged by size:
2349 601 507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 733 733
Size: 3713
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 727 1757 727
Size: 1030 2683
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BbsI GAAGACnn'nnnn_
Cuts at: 650 650
Size: 3713
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 1233 1233
Size: 3713
BglII A'GATC_T
Cuts at: 195 1228 3265 195
Size: 1033 2037 643
Fragments arranged by size:
2037 1033 643
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 555 1338 1431 2487 555
Size: 783 93 1056 1781
Fragments arranged by size:
1781 1056 783 93
BmrI ACTGGGnnnn_n'
Cuts at: 925 1006 1341 3485 925
Size: 81 335 2144 1153
Fragments arranged by size:
2144 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 3713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 587 230
Size: 357 3356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1768 2260 2558 889
Size: 879 492 298 2044
Fragments arranged by size:
2044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 1144 1392 2094 3590 1144
Size: 248 702 1496 1267
Fragments arranged by size:
1496 1267 702 248
BsaWI w'CCGG_w
Cuts at: 326 902 1423 2101 2375 2522 2933 326
Size: 576 521 678 274 147 411 1106
Fragments arranged by size:
1106 678 576 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 560 590 560
Size: 30 3683
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 242 181
Size: 61 3652
BseYI C'CCAG_C
Cuts at: 1138 1896 2473 2969 1138
Size: 758 577 496 1882
Fragments arranged by size:
1882 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 145
Size: 3713
BsiEI CG_ry'CG
Cuts at: 104 1255 1301 2509 104
Size: 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 46
BsiHKAI G_wGCw'C
Cuts at: 573 727 1505 1695 2487 573
Size: 154 778 190 792 1799
Fragments arranged by size:
1799 792 778 190 154
BsmI GAATG_Cn'
Cuts at: 642 642
Size: 3713
BsmBI CGTCTCn'nnnn_
Cuts at: 163 163
Size: 3713
Bsp1286I G_dGCh'C
Cuts at: 555 573 727 1338 1431 1505 1695 1757 2487
Size: 18 154 611 93 74 190 62 730
Cuts at: 2487 555
Size: 1781
Fragments arranged by size:
1781 730 611 190 154 93 74 62
18
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 3713
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 977 1279 1660 763
Size: 214 302 381 2816
Fragments arranged by size:
2816 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2005 2059 3543 3708 50
Size: 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 422 442 1625 422
Size: 20 1183 2510
Fragments arranged by size:
2510 1183 20
BsrFI r'CCGG_y
Cuts at: 1711 1892 2101 1711
Size: 181 209 3323
Fragments arranged by size:
3323 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 1090 1090
Size: 3713
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstYI r'GATC_y
Cuts at: 1 195 738 1205 1228 1563 1809 2821 2907
Size: 194 543 467 23 335 246 1012 86
Cuts at: 2907 2919 3265 1
Size: 12 346 449
Fragments arranged by size:
1012 543 467 449 346 335 246 194
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 320 320
Size: 3713
BtgI C'CryG_G
Cuts at: 107 107
Size: 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 102 1663 1844 2007 102
Size: 1561 181 163 1808
Fragments arranged by size:
1808 1561 181 163
BtsI GCAGTG_nn'
Cuts at: 956 956
Size: 3713
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 110 1298 1472 1863 1890 3235 110
Size: 1188 174 391 27 1345 588
Fragments arranged by size:
1345 1188 588 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 154 1736 1946 154
Size: 1582 210 1921
Fragments arranged by size:
1921 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 230 587 1538 1970 2717 2985 178
Size: 52 357 951 432 747 268 906
Fragments arranged by size:
951 906 747 432 357 268 52
EcoO109I rG'GnC_Cy
Cuts at: 241 852 241
Size: 611 3102
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 214 1732 214
Size: 1518 2195
FspI TGC'GCA
Cuts at: 1494 1494
Size: 3713
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 1555 1585 1587 1617 165
Size: 32 1358 30 2 30 2261
Fragments arranged by size:
2261 1358 32 30 30 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2568 2384
Size: 184 3529
MscI TGG'CCA
Cuts at: 112 1474 112
Size: 1362 2351
MslI CAynn'nnrTG
Cuts at: 121 560 1702 1829 121
Size: 439 1142 127 2005
Fragments arranged by size:
2005 1142 439 127
MspA1I CmG'CkG
Cuts at: 412 463 824 1138 1498 2511 2756 412
Size: 51 361 314 360 1013 245 1369
Fragments arranged by size:
1369 1013 361 360 314 245 51
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NdeI CA'TA_TG
Cuts at: 258 258
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 559 766 1266 2173 559
Size: 207 500 907 2099
Fragments arranged by size:
2099 907 500 207
PciI A'CATG_T
Cuts at: 555 2169 555
Size: 1614 2099
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PpuMI rG'GwC_Cy
Cuts at: 241 241
Size: 3713
PstI C_TGCA'G
Cuts at: 468 760 1445 468
Size: 292 685 2736
Fragments arranged by size:
2736 685 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 412 463 1138 1498 412
Size: 51 675 360 2627
Fragments arranged by size:
2627 675 360 51
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 154 1736 1946 154
Size: 1582 210 1921
Fragments arranged by size:
1921 1582 210
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 464 491 756 1441 2434 2625 33
Size: 431 27 265 685 993 191 1121
Fragments arranged by size:
1121 993 685 431 265 191 27
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 868 1783 2275 2537 868
Size: 915 492 262 2044
Fragments arranged by size:
2044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3713
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
SspI AAT'ATT
Cuts at: 357 357
Size: 3713
StyI C'CwwG_G
Cuts at: 107 847 1201 107
Size: 740 354 2619
Fragments arranged by size:
2619 740 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1296 1429 1888 2097 1296
Size: 133 459 209 2912
Fragments arranged by size:
2912 459 209 133
TatI w'GTAC_w
Cuts at: 506 3228 506
Size: 2722 991
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 3681
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1208 1208
Size: 3713
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
XmnI GAAnn'nnTTC
Cuts at: 695 695
Size: 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI