Restriction map for
pF1KB7926
(Circular) (Six-base) MAPSORT of: pF1KB7926.seq Check: 3494 from: 1 to: 4163
pF1KB7926 4163 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 1168 1988 2420 3167 3435 309
Size: 859 820 432 747 268 1037
Fragments arranged by size:
1037 859 820 747 432 268
AflIII A'CryG_T
Cuts at: 966 1515 2619 3596 966
Size: 549 1104 977 1533
Fragments arranged by size:
1533 1104 977 549
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 1468 3035 1468
Size: 1567 2596
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 976 1167 3516 3772 976
Size: 191 2349 256 1367
Fragments arranged by size:
2349 1367 256 191
AseI AT'TA_AT
Cuts at: 20 476 20
Size: 456 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 243 1179 1593 1841 1876 243
Size: 936 414 248 35 2530
Fragments arranged by size:
2530 936 414 248 35
BanII G_rGCy'C
Cuts at: 267 1177 2207 267
Size: 910 1030 2223
Fragments arranged by size:
2223 1030 910
BbeI G_GCGC'C
Cuts at: 247 1597 1845 247
Size: 1350 248 2565
Fragments arranged by size:
2565 1350 248
BbsI GAAGACnn'nnnn_
Cuts at: 550 550
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 182 148
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 704 1035 1683 704
Size: 331 648 3184
Fragments arranged by size:
3184 648 331
BfrBI ATG'CAT
Cuts at: 738 738
Size: 4163
BglII A'GATC_T
Cuts at: 814 1678 3715 814
Size: 864 2037 1262
Fragments arranged by size:
2037 1262 864
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 1788 1881 2937 1788
Size: 93 1056 3014
Fragments arranged by size:
3014 1056 93
BmgBI CAC'GTC
Cuts at: 1078 1078
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 397 451 898 319
Size: 78 54 447 3584
Fragments arranged by size:
3584 447 78 54
Bpu10I CC'TnA_GC
Cuts at: 293 293
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 244 1594 1842 2544 4040 244
Size: 1350 248 702 1496 367
Fragments arranged by size:
1496 1350 702 367 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 249
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 367 385 394 406 409 412 415 475
Size: 120 18 9 12 3 3 3 60
Cuts at: 475 478 1133 247
Size: 3 655 3277
Fragments arranged by size:
3277 655 120 60 18 12 9 3
3 3 3
BseYI C'CCAG_C
Cuts at: 1588 2346 2923 3419 1588
Size: 758 577 496 2332
Fragments arranged by size:
2332 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 200
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 250 250
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 267 1177 1788 1881 1955 2145 2207 2937 267
Size: 910 611 93 74 190 62 730 1493
Fragments arranged by size:
1493 910 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 786 1213 1427 1729 2110 786
Size: 427 214 302 381 2839
Fragments arranged by size:
2839 427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1103 2075 1103
Size: 972 3191
BsrFI r'CCGG_y
Cuts at: 240 440 2161 2342 2551 240
Size: 200 1721 181 209 1852
Fragments arranged by size:
1852 1721 209 200 181
BsrGI T'GTAC_A
Cuts at: 692 692
Size: 4163
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 814 1188 1655 1678 2013 2259 3271 3357
Size: 813 374 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 813 467 449 374 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 1748 1922 2313 2340 3685 1748
Size: 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 362 383 2186 2396 362
Size: 21 1803 210 2129
Fragments arranged by size:
2129 1803 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 2371 2681 2827 3973 173
Size: 2198 310 146 1146 363
Fragments arranged by size:
2198 1146 363 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 319 397 451 898 1168 1988 2420 3167
Size: 10 78 54 447 270 820 432 747
Cuts at: 3167 3435 309
Size: 268 1037
Fragments arranged by size:
1037 820 747 447 432 270 268 78
54 10
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 149 247 471 1597 1845 2867 3846 149
Size: 98 224 1126 248 1022 979 466
Fragments arranged by size:
1126 1022 979 466 248 224 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 633 665 2005 2035 2037 2067 249
Size: 32 352 32 1340 30 2 30 2345
Fragments arranged by size:
2345 1340 352 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 243 1593 1841 243
Size: 1350 248 2565
Fragments arranged by size:
2565 1350 248
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 338 647 1025 1098 2834 3018 338
Size: 309 378 73 1736 184 1483
Fragments arranged by size:
1736 1483 378 309 184 73
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 2152 2279 2152
Size: 127 4036
MspA1I CmG'CkG
Cuts at: 226 1274 1588 1948 2961 3206 226
Size: 1048 314 360 1013 245 1183
Fragments arranged by size:
1183 1048 1013 360 314 245
NaeI GCC'GGC
Cuts at: 442 2344 442
Size: 1902 2261
NarI GG'CG_CC
Cuts at: 244 1594 1842 244
Size: 1350 248 2565
Fragments arranged by size:
2565 1350 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 440 2342 440
Size: 1902 2261
NsiI A_TGCA'T
Cuts at: 740 740
Size: 4163
NspI r_CATG'y
Cuts at: 970 1216 1716 2623 970
Size: 246 500 907 2510
Fragments arranged by size:
2510 907 500 246
PciI A'CATG_T
Cuts at: 966 2619 966
Size: 1653 2510
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PshAI GACnn'nnGTC
Cuts at: 410 410
Size: 4163
PsiI TTA'TAA
Cuts at: 656 656
Size: 4163
PstI C_TGCA'G
Cuts at: 171 1210 1895 171
Size: 1039 685 2439
Fragments arranged by size:
2439 1039 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 167 600 1206 1891 2884 3075 33
Size: 134 433 606 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 433 191 134
SfoI GGC'GCC
Cuts at: 245 1595 1843 245
Size: 1350 248 2565
Fragments arranged by size:
2565 1350 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2725 2987 1318
Size: 915 492 262 2494
Fragments arranged by size:
2494 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 1297 1651 107
Size: 1190 354 2619
Fragments arranged by size:
2619 1190 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 686 1746 1879 2338 2547 686
Size: 1060 133 459 209 2302
Fragments arranged by size:
2302 1060 459 209 133
TatI w'GTAC_w
Cuts at: 633 692 3678 633
Size: 59 2986 1118
Fragments arranged by size:
2986 1118 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 779 811 3680 3712 779
Size: 32 2869 32 1230
Fragments arranged by size:
2869 1230 32 32
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 711 762 1194 711
Size: 51 432 3680
Fragments arranged by size:
3680 432 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
XmnI GAAnn'nnTTC
Cuts at: 557 557
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BglI BmtI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI