Restriction map for
pF1KB7917
(Circular) (Six-base) MAPSORT of: pF1KB7917.seq Check: 4632 from: 1 to: 4223
pF1KB7917 4223 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 605 2048 2480 3227 3495 252
Size: 353 1443 432 747 268 980
Fragments arranged by size:
1443 980 747 432 353 268
AflIII A'CryG_T
Cuts at: 677 1575 2679 3656 677
Size: 898 1104 977 1244
Fragments arranged by size:
1244 1104 977 898
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AhdI GACnn_n'nnGTC
Cuts at: 162 375 162
Size: 213 4010
AleI CACnn'nnGTG
Cuts at: 395 395
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 141 1528 3095 141
Size: 1387 1567 1269
Fragments arranged by size:
1567 1387 1269
ApaI G_GGCC'C
Cuts at: 201 201
Size: 4223
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 1113 1227 3576 3832 1113
Size: 114 2349 256 1504
Fragments arranged by size:
2349 1504 256 114
AseI AT'TA_AT
Cuts at: 20 1040 20
Size: 1020 3203
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 1243 1243
Size: 4223
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 115 643 1151 1239 1653 1901 1936 115
Size: 528 508 88 414 248 35 2402
Fragments arranged by size:
2402 528 508 414 248 88 35
BanII G_rGCy'C
Cuts at: 201 360 415 1237 2267 201
Size: 159 55 822 1030 2157
Fragments arranged by size:
2157 1030 822 159 55
BbeI G_GCGC'C
Cuts at: 119 1155 1657 1905 119
Size: 1036 502 248 2437
Fragments arranged by size:
2437 1036 502 248
BbsI GAAGACnn'nnnn_
Cuts at: 615 905 615
Size: 290 3933
BbvCI CC'TCA_GC
Cuts at: 1187 1187
Size: 4223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 227 1129 1163 193
Size: 34 902 34 3253
Fragments arranged by size:
3253 902 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 632 1743 632
Size: 1111 3112
BglI GCCn_nnn'nGGC
Cuts at: 179 179
Size: 4223
BglII A'GATC_T
Cuts at: 1738 3775 1738
Size: 2037 2186
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 201 1848 1941 2997 201
Size: 1647 93 1056 1427
Fragments arranged by size:
1647 1427 1056 93
BmgBI CAC'GTC
Cuts at: 762 762
Size: 4223
BmrI ACTGGGnnnn_n'
Cuts at: 402 1435 1516 1851 3995 402
Size: 1033 81 335 2144 630
Fragments arranged by size:
2144 1033 630 335 81
BmtI G_CTAG'C
Cuts at: 1102 1102
Size: 4223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 267 2590 267
Size: 2323 1900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 223 613 163
Size: 60 390 3773
Fragments arranged by size:
3773 390 60
Bpu10I CC'TnA_GC
Cuts at: 1187 1187
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2278 2770 3068 1399
Size: 879 492 298 2554
Fragments arranged by size:
2554 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 502 769 3927 502
Size: 267 3158 798
Fragments arranged by size:
3158 798 267
BsaAI yAC'GTr
Cuts at: 680 739 1576 680
Size: 59 837 3327
Fragments arranged by size:
3327 837 59
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 116 163 1152 1654 1902 2604 4100 116
Size: 47 989 502 248 702 1496 239
Fragments arranged by size:
1496 989 702 502 248 239 47
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 791 821 791
Size: 30 4193
BseYI C'CCAG_C
Cuts at: 176 314 698 878 1648 2406 2983 3479 176
Size: 138 384 180 770 758 577 496 920
Fragments arranged by size:
920 770 758 577 496 384 180 138
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 253 467 1237 2015 2205 2997 253
Size: 214 770 778 190 792 1479
Fragments arranged by size:
1479 792 778 770 214 190
BsmBI CGTCTCn'nnnn_
Cuts at: 768 768
Size: 4223
Bsp1286I G_dGCh'C
Cuts at: 201 253 360 415 467 1237 1848 1941 2015
Size: 52 107 55 52 770 611 93 74
Cuts at: 2015 2205 2267 2997 201
Size: 190 62 730 1427
Fragments arranged by size:
1427 770 730 611 190 107 93 74
62 55 52 52
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 1487 1789 2170 1273
Size: 214 302 381 3326
Fragments arranged by size:
3326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 362 1221 2135 362
Size: 859 914 2450
Fragments arranged by size:
2450 914 859
BsrFI r'CCGG_y
Cuts at: 2221 2402 2611 2221
Size: 181 209 3833
Fragments arranged by size:
3833 209 181
BssHII G'CGCG_C
Cuts at: 182 182
Size: 4223
BssSI C'ACGA_G
Cuts at: 772 2494 2852 772
Size: 1722 358 2143
Fragments arranged by size:
2143 1722 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1600 1600
Size: 4223
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 702 702
Size: 4223
BstYI r'GATC_y
Cuts at: 1 659 1248 1715 1738 2073 2319 3331 3417
Size: 658 589 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 658 589 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 261 733 2173 2354 2517 261
Size: 472 1440 181 163 1967
Fragments arranged by size:
1967 1440 472 181 163
BtsI GCAGTG_nn'
Cuts at: 535 1466 535
Size: 931 3292
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 724 1929 2787 724
Size: 1205 858 2160
Fragments arranged by size:
2160 1205 858
EaeI y'GGCC_r
Cuts at: 127 1808 1982 2373 2400 3745 127
Size: 1681 174 391 27 1345 605
Fragments arranged by size:
1681 1345 605 391 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 223 252 605 613 2048 2480 3227 3495
Size: 60 29 353 8 1435 432 747 268
Cuts at: 3495 163
Size: 891
Fragments arranged by size:
1435 891 747 432 353 268 60 29
8
EcoNI CCTnn'n_nnAGG
Cuts at: 712 712
Size: 4223
EcoO109I rG'GnC_Cy
Cuts at: 197 1362 197
Size: 1165 3058
EcoRI G'AATT_C
Cuts at: 1113 1227 1113
Size: 114 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 4223
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 119 237 1155 1657 1905 2927 3906 119
Size: 118 918 502 248 1022 979 436
Fragments arranged by size:
1022 979 918 502 436 248 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 361 393 791 823 2065 2095 2097
Size: 32 181 32 398 32 1242 30 2
Cuts at: 2097 2127 148
Size: 30 2244
Fragments arranged by size:
2244 1242 398 181 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 115 1151 1653 1901 115
Size: 1036 502 248 2437
Fragments arranged by size:
2437 1036 502 248
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 474 809 846 2894 3078 474
Size: 335 37 2048 184 1619
Fragments arranged by size:
2048 1619 335 184 37
MscI TGG'CCA
Cuts at: 1984 1984
Size: 4223
MslI CAynn'nnrTG
Cuts at: 395 1093 2212 2339 395
Size: 698 1119 127 2279
Fragments arranged by size:
2279 1119 698 127
MspA1I CmG'CkG
Cuts at: 141 176 314 546 1334 1648 2008 3021 3266
Size: 35 138 232 788 314 360 1013 245
Cuts at: 3266 141
Size: 1098
Fragments arranged by size:
1098 1013 788 360 314 245 232 138
35
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 116 1152 1654 1902 116
Size: 1036 502 248 2437
Fragments arranged by size:
2437 1036 502 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NheI G'CTAG_C
Cuts at: 1098 1098
Size: 4223
NspI r_CATG'y
Cuts at: 727 1276 1776 2683 727
Size: 549 500 907 2267
Fragments arranged by size:
2267 907 549 500
PasI CC'CwG_GG
Cuts at: 608 608
Size: 4223
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PflMI CCAn_nnn'nTGG
Cuts at: 141 288 141
Size: 147 4076
PfoI T'CCnGG_A
Cuts at: 239 458 239
Size: 219 4004
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PspOMI G'GGCC_C
Cuts at: 197 197
Size: 4223
PstI C_TGCA'G
Cuts at: 716 1270 1955 716
Size: 554 685 2984
Fragments arranged by size:
2984 685 554
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 141 1648 2008 141
Size: 1507 360 2356
Fragments arranged by size:
2356 1507 360
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 716 1270 716
Size: 554 3669
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SexAI A'CCwGG_T
Cuts at: 287 287
Size: 4223
SfcI C'TryA_G
Cuts at: 33 712 1266 1951 2944 3135 33
Size: 679 554 685 993 191 1121
Fragments arranged by size:
1121 993 685 679 554 191
SfoI GGC'GCC
Cuts at: 117 1153 1655 1903 117
Size: 1036 502 248 2437
Fragments arranged by size:
2437 1036 502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 1378 2293 2785 3047 1378
Size: 915 492 262 2554
Fragments arranged by size:
2554 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
StyI C'CwwG_G
Cuts at: 107 1357 1711 107
Size: 1250 354 2619
Fragments arranged by size:
2619 1250 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 1806 1939 2398 2607 589
Size: 1217 133 459 209 2205
Fragments arranged by size:
2205 1217 459 209 133
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4191
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 885 1254 885
Size: 369 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
XmnI GAAnn'nnTTC
Cuts at: 442 442
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BfrBI BseRI BsgI BsiWI BsmI BsrGI BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI