Restriction map for
pF1KB7895
(Circular) (Six-base) MAPSORT of: pF1KB7895.seq Check: 7727 from: 1 to: 4877
pF1KB7895 4877 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1421 1915 1421
Size: 494 4383
Acc65I G'GTAC_C
Cuts at: 1316 1893 1316
Size: 577 4300
AclI AA'CG_TT
Cuts at: 4717 4717
Size: 4877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1019 1568 1573 2702 3134 3881 4149 430
Size: 589 549 5 1129 432 747 268 1158
Fragments arranged by size:
1158 1129 747 589 549 432 268 5
AfeI AGC'GCT
Cuts at: 439 439
Size: 4877
AflIII A'CryG_T
Cuts at: 1567 1683 1869 2229 3333 4310 1567
Size: 116 186 360 1104 977 2134
Fragments arranged by size:
2134 1104 977 360 186 116
AgeI A'CCGG_T
Cuts at: 3265 3265
Size: 4877
AlwNI CAG_nnn'CTG
Cuts at: 1504 2182 3749 1504
Size: 678 1567 2632
Fragments arranged by size:
2632 1567 678
ApaLI G'TGCA_C
Cuts at: 3647 3647
Size: 4877
ApoI r'AATT_y
Cuts at: 1632 1660 1881 4230 4486 1632
Size: 28 221 2349 256 2023
Fragments arranged by size:
2349 2023 256 221 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 115 1897 115
Size: 1782 3095
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4877
BanI G'GyrC_C
Cuts at: 1316 1893 2307 2555 2590 1316
Size: 577 414 248 35 3603
Fragments arranged by size:
3603 577 414 248 35
BanII G_rGCy'C
Cuts at: 1891 2921 1891
Size: 1030 3847
BbeI G_GCGC'C
Cuts at: 2311 2559 2311
Size: 248 4629
BbsI GAAGACnn'nnnn_
Cuts at: 1007 1007
Size: 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1470 1504 1470
Size: 34 4843
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3536 4877 2775
Size: 761 1341 2775
Fragments arranged by size:
2775 1341 761
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4877
BfrBI ATG'CAT
Cuts at: 1071 1071
Size: 4877
BglI GCCn_nnn'nGGC
Cuts at: 1150 1150
Size: 4877
BglII A'GATC_T
Cuts at: 804 2392 4429 804
Size: 1588 2037 1252
Fragments arranged by size:
2037 1588 1252
BlpI GC'TnA_GC
Cuts at: 1989 1989
Size: 4877
Bme1580I G_kGCm'C
Cuts at: 2502 2595 3651 2502
Size: 93 1056 3728
Fragments arranged by size:
3728 1056 93
BmgBI CAC'GTC
Cuts at: 889 889
Size: 4877
BmrI ACTGGGnnnn_n'
Cuts at: 472 546 2089 2170 2505 4649 472
Size: 74 1543 81 335 2144 700
Fragments arranged by size:
2144 1543 700 335 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 4877
Bpu10I CC'TnA_GC
Cuts at: 551 551
Size: 4877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2932 3424 3722 2053
Size: 879 492 298 3208
Fragments arranged by size:
3208 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4581 4581
Size: 4877
BsaAI yAC'GTr
Cuts at: 1089 2230 1089
Size: 1141 3736
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 2308 2556 3258 4754 2308
Size: 248 702 1496 2431
Fragments arranged by size:
2431 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2066 2587 3265 3539 3686 4097 2066
Size: 521 678 274 147 411 2846
Fragments arranged by size:
2846 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 592 622 592
Size: 30 4847
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 4877
BseYI C'CCAG_C
Cuts at: 320 2302 3060 3637 4133 320
Size: 1982 758 577 496 1064
Fragments arranged by size:
1982 1064 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2419 2465 3673 104
Size: 2315 46 1208 1308
Fragments arranged by size:
2315 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1891 2669 2859 3651 1891
Size: 778 190 792 3117
Fragments arranged by size:
3117 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1857 1857
Size: 4877
Bsp1286I G_dGCh'C
Cuts at: 1891 2502 2595 2669 2859 2921 3651 1891
Size: 611 93 74 190 62 730 3117
Fragments arranged by size:
3117 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4097 4097
Size: 4877
BspHI T'CATG_A
Cuts at: 4053 4053
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1927 2141 2443 2824 1927
Size: 214 302 381 3980
Fragments arranged by size:
3980 381 302 214
BsrBI CCG'CTC
Cuts at: 50 598 3169 3223 4707 4872 50
Size: 548 2571 54 1484 165 55
Fragments arranged by size:
2571 1484 548 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2789 2789
Size: 4877
BsrFI r'CCGG_y
Cuts at: 2875 3056 3265 2875
Size: 181 209 4487
Fragments arranged by size:
4487 209 181
BsrGI T'GTAC_A
Cuts at: 1173 1678 1173
Size: 505 4372
BssSI C'ACGA_G
Cuts at: 3148 3506 3148
Size: 358 4519
BstAPI GCAn_nnn'nTGC
Cuts at: 206 2254 206
Size: 2048 2829
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstYI r'GATC_y
Cuts at: 1 679 804 1849 1902 2369 2392 2727 2973
Size: 678 125 1045 53 467 23 335 246
Cuts at: 2973 3985 4071 4083 4429 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1045 1012 678 467 449 346 335 246
125 86 53 23 12
BtgI C'CryG_G
Cuts at: 107 1294 1575 107
Size: 1187 281 3409
Fragments arranged by size:
3409 1187 281
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2827 3008 3171 2827
Size: 181 163 4533
Fragments arranged by size:
4533 181 163
BtsI GCAGTG_nn'
Cuts at: 1618 2120 1618
Size: 502 4375
DraI TTT'AAA
Cuts at: 135 1876 135
Size: 1741 3136
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3441 2583
Size: 858 4019
EaeI y'GGCC_r
Cuts at: 2462 2636 3027 3054 4399 2462
Size: 174 391 27 1345 2940
Fragments arranged by size:
2940 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 122 445 818 1544 1747 2900 3110 122
Size: 323 373 726 203 1153 210 1889
Fragments arranged by size:
1889 1153 726 373 323 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 3085 3395 3541 4687 139
Size: 2946 310 146 1146 329
Fragments arranged by size:
2946 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 1889 1889
Size: 4877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1019 1568 1573 2702 3134 3881 4149 430
Size: 589 549 5 1129 432 747 268 1158
Fragments arranged by size:
1158 1129 747 589 549 432 268 5
EcoNI CCTnn'n_nnAGG
Cuts at: 118 118
Size: 4877
EcoO109I rG'GnC_Cy
Cuts at: 2016 2016
Size: 4877
EcoRI G'AATT_C
Cuts at: 1632 1881 1632
Size: 249 4628
EcoRV GAT'ATC
Cuts at: 229 229
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 2658 2658
Size: 4877
HaeII r_GCGC'y
Cuts at: 441 2311 2559 3581 4560 441
Size: 1870 248 1022 979 758
Fragments arranged by size:
1870 1022 979 758 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 592 624 1119 1151 2719 2749 2751 2781 592
Size: 32 495 32 1568 30 2 30 2688
Fragments arranged by size:
2688 1568 495 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1053 1422 1916 1053
Size: 369 494 4014
Fragments arranged by size:
4014 494 369
HindIII A'AGCT_T
Cuts at: 1334 1334
Size: 4877
KasI G'GCGC_C
Cuts at: 2307 2555 2307
Size: 248 4629
KpnI G_GTAC'C
Cuts at: 1320 1897 1320
Size: 577 4300
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 939 3548 3732 939
Size: 2609 184 2084
Fragments arranged by size:
2609 2084 184
MscI TGG'CCA
Cuts at: 2638 2638
Size: 4877
MslI CAynn'nnrTG
Cuts at: 1195 2866 2993 1195
Size: 1671 127 3079
Fragments arranged by size:
3079 1671 127
MspA1I CmG'CkG
Cuts at: 572 1988 2302 2662 3675 3920 572
Size: 1416 314 360 1013 245 1529
Fragments arranged by size:
1529 1416 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4877
NarI GG'CG_CC
Cuts at: 2308 2556 2308
Size: 248 4629
NcoI C'CATG_G
Cuts at: 107 1294 1575 107
Size: 1187 281 3409
Fragments arranged by size:
3409 1187 281
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4877
NsiI A_TGCA'T
Cuts at: 1073 1073
Size: 4877
NspI r_CATG'y
Cuts at: 1687 1873 1930 2430 3337 1687
Size: 186 57 500 907 3227
Fragments arranged by size:
3227 907 500 186 57
PciI A'CATG_T
Cuts at: 1683 1869 3333 1683
Size: 186 1464 3227
Fragments arranged by size:
3227 1464 186
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PmlI CAC'GTG
Cuts at: 1089 1089
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4845
PspXI vC'TCGA_Gb
Cuts at: 115 115
Size: 4877
PstI C_TGCA'G
Cuts at: 1669 1924 2609 1669
Size: 255 685 3937
Fragments arranged by size:
3937 685 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 572 2302 2662 572
Size: 1730 360 2787
Fragments arranged by size:
2787 1730 360
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1891 1891
Size: 4877
SalI G'TCGA_C
Cuts at: 1420 1914 1420
Size: 494 4383
SapI GCTCTTCn'nnn_
Cuts at: 445 2900 3110 445
Size: 2455 210 2212
Fragments arranged by size:
2455 2212 210
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4877
ScaI AGT'ACT
Cuts at: 4394 4394
Size: 4877
SfcI C'TryA_G
Cuts at: 33 1139 1181 1465 1665 1920 2605 3598 3789
Size: 1106 42 284 200 255 685 993 191
Cuts at: 3789 33
Size: 1121
Fragments arranged by size:
1121 1106 993 685 284 255 200 191
42
SfoI GGC'GCC
Cuts at: 2309 2557 2309
Size: 248 4629
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 115 2032 2947 3439 3701 115
Size: 1917 915 492 262 1291
Fragments arranged by size:
1917 1291 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4377
SspI AAT'ATT
Cuts at: 193 193
Size: 4877
StyI C'CwwG_G
Cuts at: 107 387 1294 1575 2011 2365 107
Size: 280 907 281 436 354 2619
Fragments arranged by size:
2619 907 436 354 281 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2460 2593 3052 3261 2460
Size: 133 459 209 4076
Fragments arranged by size:
4076 459 209 133
TatI w'GTAC_w
Cuts at: 145 1173 1651 1678 4392 145
Size: 1028 478 27 2714 630
Fragments arranged by size:
2714 1028 630 478 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4394 4426 4394
Size: 32 4845
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2372
Size: 4877
XhoI C'TCGA_G
Cuts at: 115 115
Size: 4877
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmtI BpmI BsgI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XmnI ZraI