Restriction map for
pF1KB7880
(Circular) (Six-base) MAPSORT of: pF1KB7880.seq Check: 6470 from: 1 to: 5033
pF1KB7880 5033 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 2071 2071
Size: 5033
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 4873 4873
Size: 5033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 1704 2858 3290 4037 4305 1642
Size: 62 1154 432 747 268 2370
Fragments arranged by size:
2370 1154 747 432 268 62
AfeI AGC'GCT
Cuts at: 122 122
Size: 5033
AflII C'TTAA_G
Cuts at: 1573 1573
Size: 5033
AflIII A'CryG_T
Cuts at: 2385 3489 4466 2385
Size: 1104 977 2952
Fragments arranged by size:
2952 1104 977
AgeI A'CCGG_T
Cuts at: 3421 3421
Size: 5033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 1538 2338 3905 1538
Size: 800 1567 2666
Fragments arranged by size:
2666 1567 800
ApaLI G'TGCA_C
Cuts at: 3803 3803
Size: 5033
ApoI r'AATT_y
Cuts at: 629 995 1418 1502 2037 4386 4642 629
Size: 366 423 84 535 2349 256 1020
Fragments arranged by size:
2349 1020 535 423 366 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 5033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 2053 2053
Size: 5033
BamHI G'GATC_C
Cuts at: 2058 2058
Size: 5033
BanI G'GyrC_C
Cuts at: 254 983 1997 2049 2463 2711 2746 254
Size: 729 1014 52 414 248 35 2541
Fragments arranged by size:
2541 1014 729 414 248 52 35
BanII G_rGCy'C
Cuts at: 1051 2027 2047 3077 1051
Size: 976 20 1030 3007
Fragments arranged by size:
3007 1030 976 20
BbeI G_GCGC'C
Cuts at: 2467 2715 2467
Size: 248 4785
BbsI GAAGACnn'nnnn_
Cuts at: 1710 1710
Size: 5033
BbvCI CC'TCA_GC
Cuts at: 329 329
Size: 5033
BciVI GTATCCnnnnn_n'
Cuts at: 445 2931 3692 5033 445
Size: 2486 761 1341 445
Fragments arranged by size:
2486 1341 761 445
BclI T'GATC_A
Cuts at: 1323 2553 1323
Size: 1230 3803
BglII A'GATC_T
Cuts at: 2548 4585 2548
Size: 2037 2996
BlpI GC'TnA_GC
Cuts at: 2145 2145
Size: 5033
Bme1580I G_kGCm'C
Cuts at: 986 2658 2751 3807 986
Size: 1672 93 1056 2212
Fragments arranged by size:
2212 1672 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2245 2326 2661 4805 2245
Size: 81 335 2144 2473
Fragments arranged by size:
2473 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 854 1196 1280 1448 1532 298
Size: 556 342 84 168 84 3799
Fragments arranged by size:
3799 556 342 168 84 84
Bpu10I CC'TnA_GC
Cuts at: 329 329
Size: 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1065 1426 1663 1762 2209 3088 3580 3878 1065
Size: 361 237 99 447 879 492 298 2220
Fragments arranged by size:
2220 879 492 447 361 298 237 99
BsaI GGTCTCn'nnnn_
Cuts at: 745 4737 745
Size: 3992 1041
BsaAI yAC'GTr
Cuts at: 2386 2386
Size: 5033
BsaBI GATnn'nnATC
Cuts at: 6 433 2547 4584 6
Size: 427 2114 2037 455
Fragments arranged by size:
2114 2037 455 427
BsaHI Gr'CG_yC
Cuts at: 2464 2712 3414 4910 2464
Size: 248 702 1496 2587
Fragments arranged by size:
2587 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1930 2222 2743 3421 3695 3842 4253 1930
Size: 292 521 678 274 147 411 2710
Fragments arranged by size:
2710 678 521 411 292 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 346 610 238
Size: 108 264 4661
Fragments arranged by size:
4661 264 108
BseYI C'CCAG_C
Cuts at: 2458 3216 3793 4289 2458
Size: 758 577 496 3202
Fragments arranged by size:
3202 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1299 1803 1851 1299
Size: 504 48 4481
Fragments arranged by size:
4481 504 48
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3829 104
Size: 2471 46 1208 1308
Fragments arranged by size:
2471 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1412 1748 2047 2825 3015 3807 1051
Size: 361 336 299 778 190 792 2277
Fragments arranged by size:
2277 792 778 361 336 299 190
BsmI GAATG_Cn'
Cuts at: 1235 1534 1618 1235
Size: 299 84 4650
Fragments arranged by size:
4650 299 84
Bsp1286I G_dGCh'C
Cuts at: 986 1051 1412 1748 2027 2047 2658 2751 2825
Size: 65 361 336 279 20 611 93 74
Cuts at: 2825 3015 3077 3807 986
Size: 190 62 730 2212
Fragments arranged by size:
2212 730 611 361 336 279 190 93
74 65 62 20
BspEI T'CCGG_A
Cuts at: 4253 4253
Size: 5033
BspHI T'CATG_A
Cuts at: 264 443 4209 264
Size: 179 3766 1088
Fragments arranged by size:
3766 1088 179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 554 2083 2297 2599 2980 554
Size: 1529 214 302 381 2607
Fragments arranged by size:
2607 1529 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1475 3325 3379 4863 5028 50
Size: 1425 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 1425 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1116 1707 2945 1116
Size: 591 1238 3204
Fragments arranged by size:
3204 1238 591
BsrFI r'CCGG_y
Cuts at: 3031 3212 3421 3031
Size: 181 209 4643
Fragments arranged by size:
4643 209 181
BssSI C'ACGA_G
Cuts at: 3304 3662 3304
Size: 358 4675
BstAPI GCAn_nnn'nTGC
Cuts at: 2410 2410
Size: 5033
BstBI TT'CG_AA
Cuts at: 3394 3394
Size: 5033
BstXI CCAn_nnnn'nTGG
Cuts at: 571 571
Size: 5033
BstYI r'GATC_y
Cuts at: 1 2058 2525 2548 2883 3129 4141 4227 4239
Size: 2057 467 23 335 246 1012 86 12
Cuts at: 4239 4585 1
Size: 346 449
Fragments arranged by size:
2057 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 146 107
Size: 39 4994
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2983 3164 3327 2983
Size: 181 163 4689
Fragments arranged by size:
4689 181 163
BtsI GCAGTG_nn'
Cuts at: 609 1287 1539 1623 1791 1868 2276 609
Size: 678 252 84 168 77 408 3366
Fragments arranged by size:
3366 678 408 252 168 84 77
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 927 962 1098 1133 1353 1388 1689 1724 927
Size: 35 136 35 220 35 301 35 4236
Fragments arranged by size:
4236 301 220 136 35 35 35 35
DraI TTT'AAA
Cuts at: 2032 2032
Size: 5033
DrdI GACnn_nn'nnGTC
Cuts at: 2739 3597 2739
Size: 858 4175
EaeI y'GGCC_r
Cuts at: 110 512 1152 2618 2792 3183 3210 4555 110
Size: 402 640 1466 174 391 27 1345 588
Fragments arranged by size:
1466 1345 640 588 402 391 174 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 152 2015 3056 3266 152
Size: 1863 1041 210 1919
Fragments arranged by size:
1919 1863 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1120 3241 3551 3697 4843 1120
Size: 2121 310 146 1146 1310
Fragments arranged by size:
2121 1310 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 2045 1049
Size: 996 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 854 1196 1280 1448 1532 1642 1704 2858
Size: 556 342 84 168 84 110 62 1154
Cuts at: 2858 3290 4037 4305 298
Size: 432 747 268 1026
Fragments arranged by size:
1154 1026 747 556 432 342 268 168
110 84 84 62
EcoO109I rG'GnC_Cy
Cuts at: 231 336 397 2172 231
Size: 105 61 1775 3092
Fragments arranged by size:
3092 1775 105 61
EcoRI G'AATT_C
Cuts at: 995 1418 1502 2037 995
Size: 423 84 535 3991
Fragments arranged by size:
3991 535 423 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 5033
FspI TGC'GCA
Cuts at: 362 2814 362
Size: 2452 2581
HaeII r_GCGC'y
Cuts at: 124 2467 2715 3737 4716 124
Size: 2343 248 1022 979 441
Fragments arranged by size:
2343 1022 979 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2875 2905 2907 2937 2875
Size: 30 2 30 4971
Fragments arranged by size:
4971 30 30 2
HincII GTy'rAC
Cuts at: 2072 2072
Size: 5033
HindIII A'AGCT_T
Cuts at: 842 842
Size: 5033
KasI G'GCGC_C
Cuts at: 2463 2711 2463
Size: 248 4785
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 706 726 3704 3888 706
Size: 20 2978 184 1851
Fragments arranged by size:
2978 1851 184 20
MscI TGG'CCA
Cuts at: 514 1154 2794 514
Size: 640 1640 2753
Fragments arranged by size:
2753 1640 640
MslI CAynn'nnrTG
Cuts at: 422 1226 3022 3149 422
Size: 804 1796 127 2306
Fragments arranged by size:
2306 1796 804 127
MspA1I CmG'CkG
Cuts at: 2144 2458 2818 3831 4076 2144
Size: 314 360 1013 245 3101
Fragments arranged by size:
3101 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5033
NarI GG'CG_CC
Cuts at: 2464 2712 2464
Size: 248 4785
NcoI C'CATG_G
Cuts at: 107 146 107
Size: 39 4994
NdeI CA'TA_TG
Cuts at: 1453 1453
Size: 5033
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5033
NspI r_CATG'y
Cuts at: 1491 1877 2086 2586 3493 1491
Size: 386 209 500 907 3031
Fragments arranged by size:
3031 907 500 386 209
PciI A'CATG_T
Cuts at: 3489 3489
Size: 5033
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 5001
PpuMI rG'GwC_Cy
Cuts at: 397 397
Size: 5033
PsiI TTA'TAA
Cuts at: 848 1862 848
Size: 1014 4019
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 868 900 868
Size: 32 5001
PstI C_TGCA'G
Cuts at: 2080 2765 2080
Size: 685 4348
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 2458 2818 2458
Size: 360 4673
RsrII CG'GwC_CG
Cuts at: 3228 3228
Size: 5033
SacI G_AGCT'C
Cuts at: 1051 2047 1051
Size: 996 4037
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 2015 3056 3266 2015
Size: 1041 210 3782
Fragments arranged by size:
3782 1041 210
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 4550 4550
Size: 5033
SfcI C'TryA_G
Cuts at: 33 2076 2761 3754 3945 33
Size: 2043 685 993 191 1121
Fragments arranged by size:
2043 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2465 2713 2465
Size: 248 4785
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 2055 2055
Size: 5033
SmlI C'TyrA_G
Cuts at: 1044 1405 1573 1642 1741 2188 3103 3595 3857
Size: 361 168 69 99 447 915 492 262
Cuts at: 3857 1044
Size: 2220
Fragments arranged by size:
2220 915 492 447 361 262 168 99
69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 2086 2586 2086
Size: 500 4533
StuI AGG'CCT
Cuts at: 1185 1353 1381 1605 1857 1885 1185
Size: 168 28 224 252 28 4333
Fragments arranged by size:
4333 252 224 168 28 28
StyI C'CwwG_G
Cuts at: 107 146 2167 2521 107
Size: 39 2021 354 2619
Fragments arranged by size:
2619 2021 354 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 730 2616 2749 3208 3417 730
Size: 1886 133 459 209 2346
Fragments arranged by size:
2346 1886 459 209 133
TatI w'GTAC_w
Cuts at: 209 4548 209
Size: 4339 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4550 4582 4550
Size: 32 5001
Tth111I GACn'n_nGTC
Cuts at: 2830 2830
Size: 5033
XbaI T'CTAG_A
Cuts at: 2064 2064
Size: 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2528 2528
Size: 5033
XmaI C'CCGG_G
Cuts at: 2053 2053
Size: 5033
XmnI GAAnn'nnTTC
Cuts at: 955 1126 1297 1381 1549 1969 955
Size: 171 171 84 168 420 4019
Fragments arranged by size:
4019 420 171 171 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI