Restriction map for
pF1KB7851
(Circular) (Six-base) MAPSORT of: pF1KB7851.seq Check: 5565 from: 1 to: 4757
pF1KB7851 4757 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1497 1497
Size: 4757
AccI GT'mk_AC
Cuts at: 1070 1795 1070
Size: 725 4032
Acc65I G'GTAC_C
Cuts at: 1043 1773 1043
Size: 730 4027
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2582 3014 3761 4029 1005
Size: 1577 432 747 268 1733
Fragments arranged by size:
1733 1577 747 432 268
AflIII A'CryG_T
Cuts at: 2109 3213 4190 2109
Size: 1104 977 2676
Fragments arranged by size:
2676 1104 977
AgeI A'CCGG_T
Cuts at: 1182 3145 1182
Size: 1963 2794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1551 1551
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 671 2062 3629 671
Size: 1391 1567 1799
Fragments arranged by size:
1799 1567 1391
ApaI G_GGCC'C
Cuts at: 360 767 989 1606 1679 360
Size: 407 222 617 73 3438
Fragments arranged by size:
3438 617 407 222 73
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 268 1761 4110 4366 268
Size: 1493 2349 256 659
Fragments arranged by size:
2349 1493 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 821 1171 1522 1777 821
Size: 350 351 255 3801
Fragments arranged by size:
3801 351 350 255
BamHI G'GATC_C
Cuts at: 714 877 1782 714
Size: 163 905 3689
Fragments arranged by size:
3689 905 163
BanI G'GyrC_C
Cuts at: 751 1043 1122 1167 1518 1773 2187 2435 2470
Size: 292 79 45 351 255 414 248 35
Cuts at: 2470 751
Size: 3038
Fragments arranged by size:
3038 414 351 292 255 248 79 45
35
BanII G_rGCy'C
Cuts at: 360 767 961 989 1394 1511 1606 1679 1702
Size: 407 194 28 405 117 95 73 23
Cuts at: 1702 1771 2801 360
Size: 69 1030 2316
Fragments arranged by size:
2316 1030 407 405 194 117 95 73
69 28 23
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 118 792 1460 1583 118
Size: 674 668 123 3292
Fragments arranged by size:
3292 674 668 123
BbvCI CC'TCA_GC
Cuts at: 841 893 1084 1337 841
Size: 52 191 253 4261
Fragments arranged by size:
4261 253 191 52
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglI GCCn_nnn'nGGC
Cuts at: 650 650
Size: 4757
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 349 955 1869 349
Size: 606 914 3237
Fragments arranged by size:
3237 914 606
Bme1580I G_kGCm'C
Cuts at: 360 494 504 767 989 1127 1153 1485 1606
Size: 134 10 263 222 138 26 332 121
Cuts at: 1606 1679 2382 2475 3531 360
Size: 73 703 93 1056 1586
Fragments arranged by size:
1586 1056 703 332 263 222 138 134
121 93 73 26 10
BmgBI CAC'GTC
Cuts at: 686 686
Size: 4757
BmrI ACTGGGnnnn_n'
Cuts at: 965 1527 1969 2050 2385 4529 965
Size: 562 442 81 335 2144 1193
Fragments arranged by size:
2144 1193 562 442 335 81
BmtI G_CTAG'C
Cuts at: 1357 1357
Size: 4757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1413 3124 1413
Size: 1711 3046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 715 1228 1590 1693 1756 229
Size: 486 513 362 103 63 3230
Fragments arranged by size:
3230 513 486 362 103 63
Bpu10I CC'TnA_GC
Cuts at: 841 893 996 1084 1337 1499 1505 841
Size: 52 103 88 253 162 6 4093
Fragments arranged by size:
4093 253 162 103 88 52 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 1531 1933 2812 3304 3602 162
Size: 1369 402 879 492 298 1317
Fragments arranged by size:
1369 1317 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 1168 4461 1168
Size: 3293 1464
BsaAI yAC'GTr
Cuts at: 2110 2110
Size: 4757
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 239 919 1263 1474 2188 2436 3138 4634 239
Size: 680 344 211 714 248 702 1496 362
Fragments arranged by size:
1496 714 702 680 362 344 248 211
BsaWI w'CCGG_w
Cuts at: 741 1182 1946 2467 3145 3419 3566 3977 741
Size: 441 764 521 678 274 147 411 1521
Fragments arranged by size:
1521 764 678 521 441 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1732 1762 1732
Size: 30 4727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 1182 1429 241
Size: 941 247 3569
Fragments arranged by size:
3569 941 247
BseYI C'CCAG_C
Cuts at: 478 705 988 1127 1133 1279 1609 1669 1724
Size: 227 283 139 6 146 330 60 55
Cuts at: 1724 2182 2940 3517 4013 478
Size: 458 758 577 496 1222
Fragments arranged by size:
1222 758 577 496 458 330 283 227
146 139 60 55 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 506 1087 1343 1475 373
Size: 133 581 256 132 3655
Fragments arranged by size:
3655 581 256 133 132
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3553 104
Size: 2195 46 1208 1308
Fragments arranged by size:
2195 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1702 1742 1771 2549 2739 3531 1702
Size: 40 29 778 190 792 2928
Fragments arranged by size:
2928 792 778 190 40 29
BsmI GAATG_Cn'
Cuts at: 383 383
Size: 4757
Bsp1286I G_dGCh'C
Cuts at: 360 494 504 767 961 989 1127 1153 1394
Size: 134 10 263 194 28 138 26 241
Cuts at: 1394 1485 1511 1606 1679 1702 1742 1771 2382
Size: 91 26 95 73 23 40 29 611
Cuts at: 2382 2475 2549 2739 2801 3531 360
Size: 93 74 190 62 730 1586
Fragments arranged by size:
1586 730 611 263 241 194 190 138
134 95 93 91 74 73 62 40
29 28 26 26 23 10
BspEI T'CCGG_A
Cuts at: 741 3977 741
Size: 3236 1521
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1055 1497 1807 2021 2323 2704 1055
Size: 442 310 214 302 381 3108
Fragments arranged by size:
3108 442 381 310 302 214
BsrBI CCG'CTC
Cuts at: 50 587 1313 3049 3103 4587 4752 50
Size: 537 726 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 726 537 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 2669 242
Size: 2427 2330
BsrFI r'CCGG_y
Cuts at: 868 1182 1265 1297 2755 2936 3145 868
Size: 314 83 32 1458 181 209 2480
Fragments arranged by size:
2480 1458 314 209 181 83 32
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 2134 2134
Size: 4757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 793 930 793
Size: 137 4620
BstYI r'GATC_y
Cuts at: 1 185 714 744 877 1782 2249 2272 2607
Size: 184 529 30 133 905 467 23 335
Cuts at: 2607 2853 3865 3951 3963 4309 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 905 529 467 449 346 335 246
184 133 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1028 1028
Size: 4757
BtgI C'CryG_G
Cuts at: 1163 1718 1163
Size: 555 4202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 494 1326 2000 494
Size: 832 674 3251
Fragments arranged by size:
3251 832 674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1296 1331 1296
Size: 35 4722
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 796 2463 3321 796
Size: 1667 858 2232
Fragments arranged by size:
2232 1667 858
EaeI y'GGCC_r
Cuts at: 866 2342 2516 2907 2934 4279 866
Size: 1476 174 391 27 1345 1344
Fragments arranged by size:
1476 1345 1344 391 174 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 609 628 1215 2780 2990 609
Size: 19 587 1565 210 2376
Fragments arranged by size:
2376 1565 587 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 2965 3275 3421 4567 136
Size: 2829 310 146 1146 326
Fragments arranged by size:
2829 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 1700 1769 1700
Size: 69 4688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 715 1005 1228 1590 1693 1756 2582 3014
Size: 486 290 223 362 103 63 826 432
Cuts at: 3014 3761 4029 229
Size: 747 268 957
Fragments arranged by size:
957 826 747 486 432 362 290 268
223 103 63
EcoO109I rG'GnC_Cy
Cuts at: 357 579 763 764 985 1602 1603 1657 1675
Size: 222 184 1 221 617 1 54 18
Cuts at: 1675 1676 1896 357
Size: 1 220 3218
Fragments arranged by size:
3218 617 222 221 220 184 54 18
1 1 1
EcoRI G'AATT_C
Cuts at: 268 1761 268
Size: 1493 3264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1572 2776 1572
Size: 1204 3553
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 1140 1354 1569 2191 2439 3461 4440 1140
Size: 214 215 622 248 1022 979 1457
Fragments arranged by size:
1457 1022 979 622 248 215 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 571 582 603 647 670 679 702 2599
Size: 21 11 21 44 23 9 23 1897
Cuts at: 2599 2629 2631 2661 550
Size: 30 2 30 2646
Fragments arranged by size:
2646 1897 44 30 30 23 23 21
21 11 9 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1047 1777 1047
Size: 730 4027
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1391 3428 3612 1391
Size: 2037 184 2536
Fragments arranged by size:
2536 2037 184
MscI TGG'CCA
Cuts at: 2518 2518
Size: 4757
MslI CAynn'nnrTG
Cuts at: 466 1533 2746 2873 466
Size: 1067 1213 127 2350
Fragments arranged by size:
2350 1213 1067 127
MspA1I CmG'CkG
Cuts at: 192 705 771 992 1155 1272 1279 1609 1868
Size: 513 66 221 163 117 7 330 259
Cuts at: 1868 2182 2542 3555 3800 192
Size: 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 513 360 330 314 259 245
221 163 117 66 7
NaeI GCC'GGC
Cuts at: 1267 2938 1267
Size: 1671 3086
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 1163 1163
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 1265 2936 1265
Size: 1671 3086
NheI G'CTAG_C
Cuts at: 1353 1353
Size: 4757
NspI r_CATG'y
Cuts at: 1542 1717 1810 2310 3217 1542
Size: 175 93 500 907 3082
Fragments arranged by size:
3082 907 500 175 93
PasI CC'CwG_GG
Cuts at: 1111 1111
Size: 4757
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4757
PfoI T'CCnGG_A
Cuts at: 140 206 873 140
Size: 66 667 4024
Fragments arranged by size:
4024 667 66
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 579 579
Size: 4757
PspOMI G'GGCC_C
Cuts at: 356 763 985 1602 1675 356
Size: 407 222 617 73 3438
Fragments arranged by size:
3438 617 407 222 73
PstI C_TGCA'G
Cuts at: 1052 1804 2489 1052
Size: 752 685 3320
Fragments arranged by size:
3320 752 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 192 1279 2182 2542 192
Size: 1087 903 360 2407
Fragments arranged by size:
2407 1087 903 360
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1702 1771 1702
Size: 69 4688
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 609 2780 2990 609
Size: 2171 210 2376
Fragments arranged by size:
2376 2171 210
SbfI CC_TGCA'GG
Cuts at: 1052 1804 1052
Size: 752 4005
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SfcI C'TryA_G
Cuts at: 33 672 1048 1800 2485 3478 3669 33
Size: 639 376 752 685 993 191 1121
Fragments arranged by size:
1121 993 752 685 639 376 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1524 1779 1524
Size: 255 4502
SmlI C'TyrA_G
Cuts at: 177 1546 1912 2827 3319 3581 177
Size: 1369 366 915 492 262 1353
Fragments arranged by size:
1369 1353 915 492 366 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1542 1717 1810 2310 1542
Size: 175 93 500 3989
Fragments arranged by size:
3989 500 175 93
StyI C'CwwG_G
Cuts at: 398 1163 1687 1891 2245 398
Size: 765 524 204 354 2910
Fragments arranged by size:
2910 765 524 354 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 940 1075 2340 2473 2932 3141 940
Size: 135 1265 133 459 209 2556
Fragments arranged by size:
2556 1265 459 209 135 133
TatI w'GTAC_w
Cuts at: 4272 4272
Size: 4757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1732 1764 4274 4306 1732
Size: 32 2510 32 2183
Fragments arranged by size:
2510 2183 32 32
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XmaI C'CCGG_G
Cuts at: 1522 1777 1522
Size: 255 4502
XmnI GAAnn'nnTTC
Cuts at: 409 409
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI