Restriction map for
pF1KB7844
(Circular) (Six-base) MAPSORT of: pF1KB7844.seq Check: 7758 from: 1 to: 4874
pF1KB7844 4874 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1912 1912
Size: 4874
Acc65I G'GTAC_C
Cuts at: 1890 1890
Size: 4874
AclI AA'CG_TT
Cuts at: 4714 4714
Size: 4874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1695 2699 3131 3878 4146 1695
Size: 1004 432 747 268 2423
Fragments arranged by size:
2423 1004 747 432 268
AflIII A'CryG_T
Cuts at: 2226 3330 4307 2226
Size: 1104 977 2793
Fragments arranged by size:
2793 1104 977
AgeI A'CCGG_T
Cuts at: 3262 3262
Size: 4874
AlwNI CAG_nnn'CTG
Cuts at: 1014 2179 3746 1014
Size: 1165 1567 2142
Fragments arranged by size:
2142 1567 1165
ApaI G_GGCC'C
Cuts at: 1190 1190
Size: 4874
ApaLI G'TGCA_C
Cuts at: 3644 3644
Size: 4874
ApoI r'AATT_y
Cuts at: 382 417 1855 1878 4227 4483 382
Size: 35 1438 23 2349 256 773
Fragments arranged by size:
2349 1438 773 256 35 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4874
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
AvaI C'yCGr_G
Cuts at: 1894 1894
Size: 4874
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 171 204 171
Size: 33 4841
BamHI G'GATC_C
Cuts at: 1899 1899
Size: 4874
BanI G'GyrC_C
Cuts at: 1224 1651 1890 2304 2552 2587 1224
Size: 427 239 414 248 35 3511
Fragments arranged by size:
3511 427 414 248 239 35
BanII G_rGCy'C
Cuts at: 1190 1888 2918 1190
Size: 698 1030 3146
Fragments arranged by size:
3146 1030 698
BbeI G_GCGC'C
Cuts at: 1655 2308 2556 1655
Size: 653 248 3973
Fragments arranged by size:
3973 653 248
BciVI GTATCCnnnnn_n'
Cuts at: 1348 2772 3533 4874 1348
Size: 1424 761 1341 1348
Fragments arranged by size:
1424 1348 1341 761
BclI T'GATC_A
Cuts at: 2394 2394
Size: 4874
BfrBI ATG'CAT
Cuts at: 1693 1693
Size: 4874
BglI GCCn_nnn'nGGC
Cuts at: 1222 1222
Size: 4874
BglII A'GATC_T
Cuts at: 2389 4426 2389
Size: 2037 2837
BlpI GC'TnA_GC
Cuts at: 1986 1986
Size: 4874
Bme1580I G_kGCm'C
Cuts at: 1190 2499 2592 3648 1190
Size: 1309 93 1056 2416
Fragments arranged by size:
2416 1309 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2086 2167 2502 4646 2086
Size: 81 335 2144 2314
Fragments arranged by size:
2314 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 4874
Bpu10I CC'TnA_GC
Cuts at: 1275 1275
Size: 4874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1629 2050 2929 3421 3719 1179
Size: 450 421 879 492 298 2334
Fragments arranged by size:
2334 879 492 450 421 298
BsaI GGTCTCn'nnnn_
Cuts at: 894 1038 1063 4578 894
Size: 144 25 3515 1190
Fragments arranged by size:
3515 1190 144 25
BsaAI yAC'GTr
Cuts at: 2227 2227
Size: 4874
BsaBI GATnn'nnATC
Cuts at: 6 2388 4425 6
Size: 2382 2037 455
Fragments arranged by size:
2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 1652 2305 2553 3255 4751 1652
Size: 653 248 702 1496 1775
Fragments arranged by size:
1775 1496 702 653 248
BsaWI w'CCGG_w
Cuts at: 115 2063 2584 3262 3536 3683 4094 115
Size: 1948 521 678 274 147 411 895
Fragments arranged by size:
1948 895 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1043 1073 1043
Size: 30 4844
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 719 722 1051 719
Size: 3 329 4542
Fragments arranged by size:
4542 329 3
BseYI C'CCAG_C
Cuts at: 2299 3057 3634 4130 2299
Size: 758 577 496 3043
Fragments arranged by size:
3043 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1739 1739
Size: 4874
BsiEI CG_ry'CG
Cuts at: 104 2416 2462 3670 104
Size: 2312 46 1208 1308
Fragments arranged by size:
2312 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 224 648 1888 2666 2856 3648 224
Size: 424 1240 778 190 792 1450
Fragments arranged by size:
1450 1240 792 778 424 190
BsmI GAATG_Cn'
Cuts at: 173 209 173
Size: 36 4838
BsmBI CGTCTCn'nnnn_
Cuts at: 356 356
Size: 4874
Bsp1286I G_dGCh'C
Cuts at: 224 648 1190 1888 2499 2592 2666 2856 2918
Size: 424 542 698 611 93 74 190 62
Cuts at: 2918 3648 224
Size: 730 1450
Fragments arranged by size:
1450 730 698 611 542 424 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4094 4094
Size: 4874
BspHI T'CATG_A
Cuts at: 336 1116 4050 336
Size: 780 2934 1160
Fragments arranged by size:
2934 1160 780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1924 2138 2440 2821 1924
Size: 214 302 381 3977
Fragments arranged by size:
3977 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3166 3220 4704 4869 50
Size: 3116 54 1484 165 55
Fragments arranged by size:
3116 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2786 2786
Size: 4874
BsrFI r'CCGG_y
Cuts at: 2872 3053 3262 2872
Size: 181 209 4484
Fragments arranged by size:
4484 209 181
BsrGI T'GTAC_A
Cuts at: 813 813
Size: 4874
BssSI C'ACGA_G
Cuts at: 3145 3503 3145
Size: 358 4516
BstAPI GCAn_nnn'nTGC
Cuts at: 2251 2251
Size: 4874
BstBI TT'CG_AA
Cuts at: 3235 3235
Size: 4874
BstYI r'GATC_y
Cuts at: 1 1702 1899 2366 2389 2724 2970 3982 4068
Size: 1701 197 467 23 335 246 1012 86
Cuts at: 4068 4080 4426 1
Size: 12 346 449
Fragments arranged by size:
1701 1012 467 449 346 335 246 197
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4874
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2824 3005 3168 2824
Size: 181 163 4530
Fragments arranged by size:
4530 181 163
BtsI GCAGTG_nn'
Cuts at: 1524 2117 1524
Size: 593 4281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 672 707 672
Size: 35 4839
DraI TTT'AAA
Cuts at: 1376 1873 1376
Size: 497 4377
DrdI GACnn_nn'nnGTC
Cuts at: 2580 3438 2580
Size: 858 4016
EaeI y'GGCC_r
Cuts at: 2459 2633 3024 3051 4396 2459
Size: 174 391 27 1345 2937
Fragments arranged by size:
2937 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2459 2459
Size: 4874
EarI CTCTTCn'nnn_
Cuts at: 611 1638 2897 3107 611
Size: 1027 1259 210 2378
Fragments arranged by size:
2378 1259 1027 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3082 3392 3538 4684 3082
Size: 310 146 1146 3272
Fragments arranged by size:
3272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1886 1886
Size: 4874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1695 2699 3131 3878 4146 1695
Size: 1004 432 747 268 2423
Fragments arranged by size:
2423 1004 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2013 2013
Size: 4874
EcoRI G'AATT_C
Cuts at: 1878 1878
Size: 4874
EcoRV GAT'ATC
Cuts at: 943 943
Size: 4874
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 4874
FspI TGC'GCA
Cuts at: 2655 2655
Size: 4874
HaeII r_GCGC'y
Cuts at: 1655 2308 2556 3578 4557 1655
Size: 653 248 1022 979 1972
Fragments arranged by size:
1972 1022 979 653 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 1043 1075 1527 1548 1559 1580 2716
Size: 32 423 32 452 21 11 21 1136
Cuts at: 2716 2746 2748 2778 588
Size: 30 2 30 2684
Fragments arranged by size:
2684 1136 452 423 32 32 30 30
21 21 11 2
HincII GTy'rAC
Cuts at: 1913 1913
Size: 4874
KasI G'GCGC_C
Cuts at: 1651 2304 2552 1651
Size: 653 248 3973
Fragments arranged by size:
3973 653 248
KpnI G_GTAC'C
Cuts at: 1894 1894
Size: 4874
MfeI C'AATT_G
Cuts at: 1070 1831 1070
Size: 761 4113
MluI A'CGCG_T
Cuts at: 4307 4307
Size: 4874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 1401 3545 3729 327
Size: 1074 2144 184 1472
Fragments arranged by size:
2144 1472 1074 184
MscI TGG'CCA
Cuts at: 2635 2635
Size: 4874
MslI CAynn'nnrTG
Cuts at: 335 2863 2990 335
Size: 2528 127 2219
Fragments arranged by size:
2528 2219 127
MspA1I CmG'CkG
Cuts at: 516 1985 2299 2659 3672 3917 516
Size: 1469 314 360 1013 245 1473
Fragments arranged by size:
1473 1469 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3055 3055
Size: 4874
NarI GG'CG_CC
Cuts at: 1652 2305 2553 1652
Size: 653 248 3973
Fragments arranged by size:
3973 653 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4874
NdeI CA'TA_TG
Cuts at: 1851 1851
Size: 4874
NgoMIV G'CCGG_C
Cuts at: 3053 3053
Size: 4874
NsiI A_TGCA'T
Cuts at: 1695 1695
Size: 4874
NspI r_CATG'y
Cuts at: 1482 1927 2427 3334 1482
Size: 445 500 907 3022
Fragments arranged by size:
3022 907 500 445
PciI A'CATG_T
Cuts at: 3330 3330
Size: 4874
PflMI CCAn_nnn'nTGG
Cuts at: 420 1054 420
Size: 634 4240
PmeI GTTT'AAAC
Cuts at: 1873 1873
Size: 4874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4842
PspOMI G'GGCC_C
Cuts at: 1186 1186
Size: 4874
PstI C_TGCA'G
Cuts at: 361 660 1921 2606 361
Size: 299 1261 685 2629
Fragments arranged by size:
2629 1261 685 299
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4874
PvuII CAG'CTG
Cuts at: 516 2299 2659 516
Size: 1783 360 2731
Fragments arranged by size:
2731 1783 360
RsrII CG'GwC_CG
Cuts at: 3069 3069
Size: 4874
SacI G_AGCT'C
Cuts at: 1888 1888
Size: 4874
SalI G'TCGA_C
Cuts at: 1911 1911
Size: 4874
SapI GCTCTTCn'nnn_
Cuts at: 611 2897 3107 611
Size: 2286 210 2378
Fragments arranged by size:
2378 2286 210
SbfI CC_TGCA'GG
Cuts at: 1921 1921
Size: 4874
ScaI AGT'ACT
Cuts at: 1761 4391 1761
Size: 2630 2244
SexAI A'CCwGG_T
Cuts at: 1053 1053
Size: 4874
SfcI C'TryA_G
Cuts at: 33 357 656 1142 1917 2602 3595 3786 33
Size: 324 299 486 775 685 993 191 1121
Fragments arranged by size:
1121 993 775 685 486 324 299 191
SfoI GGC'GCC
Cuts at: 1653 2306 2554 1653
Size: 653 248 3973
Fragments arranged by size:
3973 653 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
SmaI CCC'GGG
Cuts at: 1896 1896
Size: 4874
SmlI C'TyrA_G
Cuts at: 1194 1608 2029 2944 3436 3698 1194
Size: 414 421 915 492 262 2370
Fragments arranged by size:
2370 915 492 421 414 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4874
SphI G_CATG'C
Cuts at: 1927 2427 1927
Size: 500 4374
SspI AAT'ATT
Cuts at: 719 719
Size: 4874
StyI C'CwwG_G
Cuts at: 107 2008 2362 107
Size: 1901 354 2619
Fragments arranged by size:
2619 1901 354
SwaI ATTT'AAAT
Cuts at: 1376 1376
Size: 4874
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2457 2590 3049 3258 2457
Size: 133 459 209 4073
Fragments arranged by size:
4073 459 209 133
TatI w'GTAC_w
Cuts at: 813 1759 4389 813
Size: 946 2630 1298
Fragments arranged by size:
2630 1298 946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 660 692 4391 4423 660
Size: 32 3699 32 1111
Fragments arranged by size:
3699 1111 32 32
Tth111I GACn'n_nGTC
Cuts at: 1055 1417 2671 1055
Size: 362 1254 3258
Fragments arranged by size:
3258 1254 362
XbaI T'CTAG_A
Cuts at: 1905 1905
Size: 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 4874
XmaI C'CCGG_G
Cuts at: 1894 1894
Size: 4874
XmnI GAAnn'nnTTC
Cuts at: 719 1859 719
Size: 1140 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BcgI BmgBI BmtI BpmI
BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
XhoI ZraI