Restriction map for
pF1KB7840
(Circular) (Six-base) MAPSORT of: pF1KB7840.seq Check: 7459 from: 1 to: 4958
pF1KB7840 4958 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AatII G_ACGT'C
Cuts at: 1695 1695
Size: 4958
AccI GT'mk_AC
Cuts at: 818 1996 818
Size: 1178 3780
Acc65I G'GTAC_C
Cuts at: 1974 1974
Size: 4958
AclI AA'CG_TT
Cuts at: 4798 4798
Size: 4958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2783 3215 3962 4230 1574
Size: 1209 432 747 268 2302
Fragments arranged by size:
2302 1209 747 432 268
AfeI AGC'GCT
Cuts at: 973 973
Size: 4958
AflII C'TTAA_G
Cuts at: 406 406
Size: 4958
AflIII A'CryG_T
Cuts at: 1524 2310 3414 4391 1524
Size: 786 1104 977 2091
Fragments arranged by size:
2091 1104 977 786
AgeI A'CCGG_T
Cuts at: 3346 3346
Size: 4958
AleI CACnn'nnGTG
Cuts at: 527 527
Size: 4958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 558 1121 1831 1855 558
Size: 563 710 24 3661
Fragments arranged by size:
3661 710 563 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1418 1450 1418
Size: 32 4926
AlwNI CAG_nnn'CTG
Cuts at: 504 777 1372 2263 3830 504
Size: 273 595 891 1567 1632
Fragments arranged by size:
1632 1567 891 595 273
ApaI G_GGCC'C
Cuts at: 1149 1629 1149
Size: 480 4478
ApaLI G'TGCA_C
Cuts at: 280 894 1580 3728 280
Size: 614 686 2148 1510
Fragments arranged by size:
2148 1510 686 614
ApoI r'AATT_y
Cuts at: 1962 4311 4567 1962
Size: 2349 256 2353
Fragments arranged by size:
2353 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
AvaI C'yCGr_G
Cuts at: 1196 1447 1928 1978 1196
Size: 251 481 50 4176
Fragments arranged by size:
4176 481 251 50
AvrII C'CTAG_G
Cuts at: 1797 1797
Size: 4958
BamHI G'GATC_C
Cuts at: 1983 1983
Size: 4958
BanI G'GyrC_C
Cuts at: 131 288 393 730 740 1339 1974 2388 2636
Size: 157 105 337 10 599 635 414 248
Cuts at: 2636 2671 131
Size: 35 2418
Fragments arranged by size:
2418 635 599 414 337 248 157 105
35 10
BanII G_rGCy'C
Cuts at: 578 1149 1223 1382 1404 1436 1629 1972 3002
Size: 571 74 159 22 32 193 343 1030
Cuts at: 3002 578
Size: 2534
Fragments arranged by size:
2534 1030 571 343 193 159 74 32
22
BbeI G_GCGC'C
Cuts at: 135 2392 2640 135
Size: 2257 248 2453
Fragments arranged by size:
2453 2257 248
BbvCI CC'TCA_GC
Cuts at: 905 905
Size: 4958
BciVI GTATCCnnnnn_n'
Cuts at: 2856 3617 4958 2856
Size: 761 1341 2856
Fragments arranged by size:
2856 1341 761
BclI T'GATC_A
Cuts at: 2478 2478
Size: 4958
BglI GCCn_nnn'nGGC
Cuts at: 722 722
Size: 4958
BglII A'GATC_T
Cuts at: 239 2473 4510 239
Size: 2234 2037 687
Fragments arranged by size:
2234 2037 687
BlpI GC'TnA_GC
Cuts at: 2070 2070
Size: 4958
Bme1580I G_kGCm'C
Cuts at: 211 284 398 898 1149 1584 1629 2583 2676
Size: 73 114 500 251 435 45 954 93
Cuts at: 2676 3732 211
Size: 1056 1437
Fragments arranged by size:
1437 1056 954 500 435 251 114 93
73 45
BmrI ACTGGGnnnn_n'
Cuts at: 662 1438 2170 2251 2586 4730 662
Size: 776 732 81 335 2144 890
Fragments arranged by size:
2144 890 776 732 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1398 1418 3325 1398
Size: 20 1907 3031
Fragments arranged by size:
3031 1907 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 252 1214 1272 1728 1740 137
Size: 115 962 58 456 12 3355
Fragments arranged by size:
3355 962 456 115 58 12
Bpu10I CC'TnA_GC
Cuts at: 905 1847 905
Size: 942 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2134 3013 3505 3803 2134
Size: 879 492 298 3289
Fragments arranged by size:
3289 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1318 1858 4662 1318
Size: 540 2804 1614
Fragments arranged by size:
2804 1614 540
BsaAI yAC'GTr
Cuts at: 767 2311 767
Size: 1544 3414
BsaBI GATnn'nnATC
Cuts at: 6 2472 4509 6
Size: 2466 2037 455
Fragments arranged by size:
2466 2037 455
BsaHI Gr'CG_yC
Cuts at: 132 828 1692 2389 2637 3339 4835 132
Size: 696 864 697 248 702 1496 255
Fragments arranged by size:
1496 864 702 697 696 255 248
BsaWI w'CCGG_w
Cuts at: 2147 2668 3346 3620 3767 4178 2147
Size: 521 678 274 147 411 2927
Fragments arranged by size:
2927 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 1399 1418 1429 1448 334
Size: 30 1035 19 11 19 3844
Fragments arranged by size:
3844 1035 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 394 574 682 1390 283
Size: 111 180 108 708 3851
Fragments arranged by size:
3851 708 180 111 108
BseYI C'CCAG_C
Cuts at: 1152 1592 1630 2383 3141 3718 4214 1152
Size: 440 38 753 758 577 496 1896
Fragments arranged by size:
1896 758 753 577 496 440 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 528 740 1851 490
Size: 38 212 1111 3597
Fragments arranged by size:
3597 1111 212 38
BsiEI CG_ry'CG
Cuts at: 104 1509 2500 2546 3754 104
Size: 1405 991 46 1208 1308
Fragments arranged by size:
1405 1308 1208 991 46
BsiHKAI G_wGCw'C
Cuts at: 284 578 898 1058 1519 1584 1972 2750 2940
Size: 294 320 160 461 65 388 778 190
Cuts at: 2940 3732 284
Size: 792 1510
Fragments arranged by size:
1510 792 778 461 388 320 294 190
160 65
BsmI GAATG_Cn'
Cuts at: 847 847
Size: 4958
Bsp1286I G_dGCh'C
Cuts at: 211 284 398 578 898 1058 1149 1223 1382
Size: 73 114 180 320 160 91 74 159
Cuts at: 1382 1404 1436 1519 1584 1629 1972 2583 2676
Size: 22 32 83 65 45 343 611 93
Cuts at: 2676 2750 2940 3002 3732 211
Size: 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 343 320 190 180 160
159 114 93 91 83 74 74 73
65 62 45 32 22
BspEI T'CCGG_A
Cuts at: 4178 4178
Size: 4958
BspHI T'CATG_A
Cuts at: 4134 4134
Size: 4958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 1490 2008 2222 2524 2905 408
Size: 1082 518 214 302 381 2461
Fragments arranged by size:
2461 1082 518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 137 3250 3304 4788 4953 50
Size: 87 3113 54 1484 165 55
Fragments arranged by size:
3113 1484 165 87 55 54
BsrDI GCAATG_nn'
Cuts at: 249 1865 2870 249
Size: 1616 1005 2337
Fragments arranged by size:
2337 1616 1005
BsrFI r'CCGG_y
Cuts at: 1258 2956 3137 3346 1258
Size: 1698 181 209 2870
Fragments arranged by size:
2870 1698 209 181
BssSI C'ACGA_G
Cuts at: 3229 3587 3229
Size: 358 4600
BstAPI GCAn_nnn'nTGC
Cuts at: 279 288 504 513 709 2335 279
Size: 9 216 9 196 1626 2902
Fragments arranged by size:
2902 1626 216 196 9 9
BstBI TT'CG_AA
Cuts at: 3319 3319
Size: 4958
BstEII G'GTnAC_C
Cuts at: 642 642
Size: 4958
BstYI r'GATC_y
Cuts at: 1 239 1983 2450 2473 2808 3054 4066 4152
Size: 238 1744 467 23 335 246 1012 86
Cuts at: 4152 4164 4510 1
Size: 12 346 449
Fragments arranged by size:
1744 1012 467 449 346 335 246 238
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1373 1373
Size: 4958
BtgI C'CryG_G
Cuts at: 225 262 225
Size: 37 4921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 684 2908 3089 3252 684
Size: 2224 181 163 2390
Fragments arranged by size:
2390 2224 181 163
BtsI GCAGTG_nn'
Cuts at: 211 284 710 999 1446 1853 2201 211
Size: 73 426 289 447 407 348 2968
Fragments arranged by size:
2968 447 426 407 348 289 73
DraI TTT'AAA
Cuts at: 1957 1957
Size: 4958
DraIII CAC_nnn'GTG
Cuts at: 288 288
Size: 4958
DrdI GACnn_nn'nnGTC
Cuts at: 2664 3522 2664
Size: 858 4100
EaeI y'GGCC_r
Cuts at: 1470 2543 2717 3108 3135 4480 1470
Size: 1073 174 391 27 1345 1948
Fragments arranged by size:
1948 1345 1073 391 174 27
EagI C'GGCC_G
Cuts at: 2543 2543
Size: 4958
EarI CTCTTCn'nnn_
Cuts at: 551 2981 3191 551
Size: 2430 210 2318
Fragments arranged by size:
2430 2318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3166 3476 3622 4768 3166
Size: 310 146 1146 3356
Fragments arranged by size:
3356 1146 310 146
EcoICRI GAG'CTC
Cuts at: 576 1970 576
Size: 1394 3564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 252 1214 1272 1574 1728 1740 2783 3215
Size: 115 962 58 302 154 12 1043 432
Cuts at: 3215 3962 4230 137
Size: 747 268 865
Fragments arranged by size:
1043 962 865 747 432 302 268 154
115 58 12
EcoNI CCTnn'n_nnAGG
Cuts at: 445 445
Size: 4958
EcoO109I rG'GnC_Cy
Cuts at: 122 165 456 1146 1369 1625 1626 2097 122
Size: 43 291 690 223 256 1 471 2983
Fragments arranged by size:
2983 690 471 291 256 223 43 1
EcoRI G'AATT_C
Cuts at: 1962 1962
Size: 4958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4958
FspI TGC'GCA
Cuts at: 631 2739 631
Size: 2108 2850
FspAI rTGC'GCAy
Cuts at: 631 631
Size: 4958
HaeII r_GCGC'y
Cuts at: 135 342 975 1360 1599 2392 2640 3662 4641
Size: 207 633 385 239 793 248 1022 979
Cuts at: 4641 135
Size: 452
Fragments arranged by size:
1022 979 793 633 452 385 248 239
207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 1169 1201 1702 1720 1734 1752 1793
Size: 32 791 32 501 18 14 18 41
Cuts at: 1793 1825 1905 1937 2800 2830 2832 2862 346
Size: 32 80 32 863 30 2 30 2442
Fragments arranged by size:
2442 863 791 501 80 41 32 32
32 32 30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 468 786 1997 468
Size: 318 1211 3429
Fragments arranged by size:
3429 1211 318
HindIII A'AGCT_T
Cuts at: 953 953
Size: 4958
KasI G'GCGC_C
Cuts at: 131 2388 2636 131
Size: 2257 248 2453
Fragments arranged by size:
2453 2257 248
KpnI G_GTAC'C
Cuts at: 1978 1978
Size: 4958
MluI A'CGCG_T
Cuts at: 1524 4391 1524
Size: 2867 2091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 3629 3813 377
Size: 3252 184 1522
Fragments arranged by size:
3252 1522 184
MscI TGG'CCA
Cuts at: 2719 2719
Size: 4958
MslI CAynn'nnrTG
Cuts at: 527 703 1103 1858 2947 3074 527
Size: 176 400 755 1089 127 2411
Fragments arranged by size:
2411 1089 755 400 176 127
MspA1I CmG'CkG
Cuts at: 264 437 501 513 597 1118 1250 2069 2383
Size: 173 64 12 84 521 132 819 314
Cuts at: 2383 2743 3756 4001 264
Size: 360 1013 245 1221
Fragments arranged by size:
1221 1013 819 521 360 314 245 173
132 84 64 12
NaeI GCC'GGC
Cuts at: 1260 3139 1260
Size: 1879 3079
NarI GG'CG_CC
Cuts at: 132 2389 2637 132
Size: 2257 248 2453
Fragments arranged by size:
2453 2257 248
NgoMIV G'CCGG_C
Cuts at: 1258 3137 1258
Size: 1879 3079
NspI r_CATG'y
Cuts at: 277 1108 2011 2511 3418 277
Size: 831 903 500 907 1817
Fragments arranged by size:
1817 907 903 831 500
PasI CC'CwG_GG
Cuts at: 1622 1895 1622
Size: 273 4685
PciI A'CATG_T
Cuts at: 3414 3414
Size: 4958
PfoI T'CCnGG_A
Cuts at: 124 124
Size: 4958
PmeI GTTT'AAAC
Cuts at: 1957 1957
Size: 4958
PmlI CAC'GTG
Cuts at: 767 767
Size: 4958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1418 1450 4097 4129 1418
Size: 32 2647 32 2247
Fragments arranged by size:
2647 2247 32 32
PpuMI rG'GwC_Cy
Cuts at: 122 165 456 122
Size: 43 291 4624
Fragments arranged by size:
4624 291 43
PspOMI G'GGCC_C
Cuts at: 1145 1625 1145
Size: 480 4478
PstI C_TGCA'G
Cuts at: 1207 2005 2690 1207
Size: 798 685 3475
Fragments arranged by size:
3475 798 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4958
PvuII CAG'CTG
Cuts at: 437 501 513 1250 2383 2743 437
Size: 64 12 737 1133 360 2652
Fragments arranged by size:
2652 1133 737 360 64 12
RsrII CG'GwC_CG
Cuts at: 3153 3153
Size: 4958
SacI G_AGCT'C
Cuts at: 578 1972 578
Size: 1394 3564
SacII CC_GC'GG
Cuts at: 265 265
Size: 4958
SalI G'TCGA_C
Cuts at: 1995 1995
Size: 4958
SanDI GG'GwC_CC
Cuts at: 122 122
Size: 4958
SapI GCTCTTCn'nnn_
Cuts at: 2981 3191 2981
Size: 210 4748
SbfI CC_TGCA'GG
Cuts at: 2005 2005
Size: 4958
ScaI AGT'ACT
Cuts at: 541 1036 4475 541
Size: 495 3439 1024
Fragments arranged by size:
3439 1024 495
SfcI C'TryA_G
Cuts at: 33 195 1128 1203 1364 2001 2686 3679 3870
Size: 162 933 75 161 637 685 993 191
Cuts at: 3870 33
Size: 1121
Fragments arranged by size:
1121 993 933 685 637 191 162 161
75
SfoI GGC'GCC
Cuts at: 133 2390 2638 133
Size: 2257 248 2453
Fragments arranged by size:
2453 2257 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
SmaI CCC'GGG
Cuts at: 1980 1980
Size: 4958
SmlI C'TyrA_G
Cuts at: 406 1196 2113 3028 3520 3782 406
Size: 790 917 915 492 262 1582
Fragments arranged by size:
1582 917 915 790 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4958
SphI G_CATG'C
Cuts at: 277 1108 2011 2511 277
Size: 831 903 500 2724
Fragments arranged by size:
2724 903 831 500
StuI AGG'CCT
Cuts at: 404 725 1181 404
Size: 321 456 4181
Fragments arranged by size:
4181 456 321
StyI C'CwwG_G
Cuts at: 1071 1209 1797 2092 2446 1071
Size: 138 588 295 354 3583
Fragments arranged by size:
3583 588 354 295 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 718 758 1495 1635 1697 2541 2674 3133
Size: 337 40 737 140 62 844 133 459
Cuts at: 3133 3342 381
Size: 209 1997
Fragments arranged by size:
1997 844 737 459 337 209 140 133
62 40
TatI w'GTAC_w
Cuts at: 539 1034 4473 539
Size: 495 3439 1024
Fragments arranged by size:
3439 1024 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 1399 1431 1563 1595 4475 4507 334
Size: 32 1033 32 132 32 2880 32 785
Fragments arranged by size:
2880 1033 785 132 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 454 2755 454
Size: 2301 2657
XbaI T'CTAG_A
Cuts at: 1989 1989
Size: 4958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1425 2453 1425
Size: 1028 3930
XhoI C'TCGA_G
Cuts at: 1196 1196
Size: 4958
XmaI C'CCGG_G
Cuts at: 1978 1978
Size: 4958
ZraI GAC'GTC
Cuts at: 1693 1693
Size: 4958
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AhdI AscI BaeI BbsI BcgI BfrBI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI EcoRV FseI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PshAI PsiI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI