Restriction map for
pF1KB7838
(Circular) (Six-base) MAPSORT of: pF1KB7838.seq Check: 7466 from: 1 to: 4955
pF1KB7838 4955 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1128 1320 1128
Size: 192 4763
AccI GT'mk_AC
Cuts at: 414 1993 414
Size: 1579 3376
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1659 1852 2780 3212 3959 4227 637
Size: 1022 193 928 432 747 268 1365
Fragments arranged by size:
1365 1022 928 747 432 268 193
AflII C'TTAA_G
Cuts at: 1522 1522
Size: 4955
AflIII A'CryG_T
Cuts at: 1283 1370 2307 3411 4388 1283
Size: 87 937 1104 977 1850
Fragments arranged by size:
1850 1104 977 937 87
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AleI CACnn'nnGTG
Cuts at: 539 1282 1732 539
Size: 743 450 3762
Fragments arranged by size:
3762 743 450
AlwNI CAG_nnn'CTG
Cuts at: 237 2260 3827 237
Size: 2023 1567 1365
Fragments arranged by size:
2023 1567 1365
ApaI G_GGCC'C
Cuts at: 862 993 862
Size: 131 4824
ApaLI G'TGCA_C
Cuts at: 544 3725 544
Size: 3181 1774
ApoI r'AATT_y
Cuts at: 1770 1959 4308 4564 1770
Size: 189 2349 256 2161
Fragments arranged by size:
2349 2161 256 189
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 525 704 986 1479 1758 1975 525
Size: 179 282 493 279 217 3505
Fragments arranged by size:
3505 493 282 279 217 179
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 1262 1308 1531 1633 1971 2385 2633 2668 1262
Size: 46 223 102 338 414 248 35 3549
Fragments arranged by size:
3549 414 338 248 223 102 46 35
BanII G_rGCy'C
Cuts at: 403 862 956 993 1815 1824 1969 2999 403
Size: 459 94 37 822 9 145 1030 2359
Fragments arranged by size:
2359 1030 822 459 145 94 37 9
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1739 1773 1739
Size: 34 4921
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3614 4955 2853
Size: 761 1341 2853
Fragments arranged by size:
2853 1341 761
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BfrBI ATG'CAT
Cuts at: 1063 1911 1063
Size: 848 4107
BglI GCCn_nnn'nGGC
Cuts at: 857 857
Size: 4955
BglII A'GATC_T
Cuts at: 1001 1409 2470 4507 1001
Size: 408 1061 2037 1449
Fragments arranged by size:
2037 1449 1061 408
BlpI GC'TnA_GC
Cuts at: 2067 2067
Size: 4955
Bme1580I G_kGCm'C
Cuts at: 548 814 862 993 1265 2580 2673 3729 548
Size: 266 48 131 272 1315 93 1056 1774
Fragments arranged by size:
1774 1315 1056 272 266 131 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 903 2167 2248 2583 4727 903
Size: 1264 81 335 2144 1131
Fragments arranged by size:
2144 1264 1131 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 1440 1672 3322 648
Size: 792 232 1650 2281
Fragments arranged by size:
2281 1650 792 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 838 1061 1777 147
Size: 691 223 716 3325
Fragments arranged by size:
3325 716 691 223
Bpu10I CC'TnA_GC
Cuts at: 950 1600 950
Size: 650 4305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2131 3010 3502 3800 1861
Size: 270 879 492 298 3016
Fragments arranged by size:
3016 879 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 1643 4659 1643
Size: 3016 1939
BsaAI yAC'GTr
Cuts at: 1284 2308 1284
Size: 1024 3931
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 143 928 1943 2386 2634 3336 4832 143
Size: 785 1015 443 248 702 1496 266
Fragments arranged by size:
1496 1015 785 702 443 266 248
BsaWI w'CCGG_w
Cuts at: 879 2144 2665 3343 3617 3764 4175 879
Size: 1265 521 678 274 147 411 1659
Fragments arranged by size:
1659 1265 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 1200 1230 145
Size: 30 1025 30 3870
Fragments arranged by size:
3870 1025 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 369 372 1185 1723 1726 162
Size: 207 3 813 538 3 3391
Fragments arranged by size:
3391 813 538 207 3 3
BseYI C'CCAG_C
Cuts at: 325 451 833 1026 1097 1151 1716 2380 3138
Size: 126 382 193 71 54 565 664 758
Cuts at: 3138 3715 4211 325
Size: 577 496 1069
Fragments arranged by size:
1069 758 664 577 565 496 382 193
126 71 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1211 1423 1211
Size: 212 4743
BsiEI CG_ry'CG
Cuts at: 104 2497 2543 3751 104
Size: 2393 46 1208 1308
Fragments arranged by size:
2393 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 463 548 1969 2747 2937 3729 463
Size: 85 1421 778 190 792 1689
Fragments arranged by size:
1689 1421 792 778 190 85
BsmI GAATG_Cn'
Cuts at: 317 1911 317
Size: 1594 3361
BsmBI CGTCTCn'nnnn_
Cuts at: 701 1792 701
Size: 1091 3864
Bsp1286I G_dGCh'C
Cuts at: 403 463 548 814 862 956 993 1265 1815
Size: 60 85 266 48 94 37 272 550
Cuts at: 1815 1824 1969 2580 2673 2747 2937 2999 3729
Size: 9 145 611 93 74 190 62 730
Cuts at: 3729 403
Size: 1629
Fragments arranged by size:
1629 730 611 550 272 266 190 145
94 93 85 74 62 60 48 37
9
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 4131 4131
Size: 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1128 1320 1661 1898 2005 2219 2521 2902 1128
Size: 192 341 237 107 214 302 381 3181
Fragments arranged by size:
3181 381 341 302 237 214 192 107
BsrBI CCG'CTC
Cuts at: 50 1757 3247 3301 4785 4950 50
Size: 1707 1490 54 1484 165 55
Fragments arranged by size:
1707 1490 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 673 2867 673
Size: 2194 2761
BsrFI r'CCGG_y
Cuts at: 1838 2953 3134 3343 1838
Size: 1115 181 209 3450
Fragments arranged by size:
3450 1115 209 181
BssSI C'ACGA_G
Cuts at: 3226 3584 3226
Size: 358 4597
BstAPI GCAn_nnn'nTGC
Cuts at: 659 753 809 2332 659
Size: 94 56 1523 3282
Fragments arranged by size:
3282 1523 94 56
BstBI TT'CG_AA
Cuts at: 3316 3316
Size: 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 783 1097 1644 783
Size: 314 547 4094
Fragments arranged by size:
4094 547 314
BstYI r'GATC_y
Cuts at: 1 937 1001 1409 1980 2447 2470 2805 3051
Size: 936 64 408 571 467 23 335 246
Cuts at: 3051 4063 4149 4161 4507 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 936 571 467 449 408 346 335
246 86 64 23 12
BtgI C'CryG_G
Cuts at: 425 854 1637 1730 425
Size: 429 783 93 3650
Fragments arranged by size:
3650 783 429 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1078 2905 3086 3249 1078
Size: 1827 181 163 2784
Fragments arranged by size:
2784 1827 181 163
BtsI GCAGTG_nn'
Cuts at: 1395 2198 1395
Size: 803 4152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 120 155 120
Size: 35 4920
DraI TTT'AAA
Cuts at: 1954 1954
Size: 4955
DrdI GACnn_nn'nnGTC
Cuts at: 2661 3519 2661
Size: 858 4097
EaeI y'GGCC_r
Cuts at: 422 785 2540 2714 3105 3132 4477 422
Size: 363 1755 174 391 27 1345 900
Fragments arranged by size:
1755 1345 900 391 363 174 27
EagI C'GGCC_G
Cuts at: 2540 2540
Size: 4955
EarI CTCTTCn'nnn_
Cuts at: 1729 1820 2978 3188 1729
Size: 91 1158 210 3496
Fragments arranged by size:
3496 1158 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3473 3619 4765 3163
Size: 310 146 1146 3353
Fragments arranged by size:
3353 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 637 838 1061 1659 1777 1852 2780 3212
Size: 490 201 223 598 118 75 928 432
Cuts at: 3212 3959 4227 147
Size: 747 268 875
Fragments arranged by size:
928 875 747 598 490 432 268 223
201 118 75
EcoNI CCTnn'n_nnAGG
Cuts at: 1522 1522
Size: 4955
EcoO109I rG'GnC_Cy
Cuts at: 850 859 1167 2094 850
Size: 9 308 927 3711
Fragments arranged by size:
3711 927 308 9
EcoRI G'AATT_C
Cuts at: 1770 1959 1770
Size: 189 4766
EcoRV GAT'ATC
Cuts at: 974 974
Size: 4955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4955
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 1702 2389 2637 3659 4638 1702
Size: 687 248 1022 979 2019
Fragments arranged by size:
2019 1022 979 687 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 508 540 958 990 2797 2827 2829
Size: 32 148 32 418 32 1807 30 2
Cuts at: 2829 2859 328
Size: 30 2424
Fragments arranged by size:
2424 1807 418 148 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 559 1274 3626 3810 149
Size: 410 715 2352 184 1294
Fragments arranged by size:
2352 1294 715 410 184
MscI TGG'CCA
Cuts at: 424 787 2716 424
Size: 363 1929 2663
Fragments arranged by size:
2663 1929 363
MslI CAynn'nnrTG
Cuts at: 539 1282 1286 1642 1732 2944 3071 539
Size: 743 4 356 90 1212 127 2423
Fragments arranged by size:
2423 1212 743 356 127 90 4
MspA1I CmG'CkG
Cuts at: 659 750 816 924 1238 1444 1732 2066 2380
Size: 91 66 108 314 206 288 334 314
Cuts at: 2380 2740 3753 3998 659
Size: 360 1013 245 1616
Fragments arranged by size:
1616 1013 360 334 314 314 288 245
206 108 91 66
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NcoI C'CATG_G
Cuts at: 425 854 1637 425
Size: 429 783 3743
Fragments arranged by size:
3743 783 429
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NsiI A_TGCA'T
Cuts at: 1065 1913 1065
Size: 848 4107
NspI r_CATG'y
Cuts at: 702 1361 1374 1911 2008 2508 3415 702
Size: 659 13 537 97 500 907 2242
Fragments arranged by size:
2242 907 659 537 500 97 13
PasI CC'CwG_GG
Cuts at: 406 406
Size: 4955
PciI A'CATG_T
Cuts at: 1370 3411 1370
Size: 2041 2914
PfoI T'CCnGG_A
Cuts at: 1793 1793
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PmlI CAC'GTG
Cuts at: 1284 1284
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4923
PspOMI G'GGCC_C
Cuts at: 858 989 858
Size: 131 4824
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 586 618 586
Size: 32 4923
PstI C_TGCA'G
Cuts at: 1625 1752 2002 2687 1625
Size: 127 250 685 3893
Fragments arranged by size:
3893 685 250 127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 924 1238 2380 2740 924
Size: 314 1142 360 3139
Fragments arranged by size:
3139 1142 360 314
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SacII CC_GC'GG
Cuts at: 1733 1733
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 1820 2978 3188 1820
Size: 1158 210 3587
Fragments arranged by size:
3587 1158 210
SbfI CC_TGCA'GG
Cuts at: 1625 2002 1625
Size: 377 4578
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SfcI C'TryA_G
Cuts at: 33 204 1614 1621 1748 1998 2683 3676 3867
Size: 171 1410 7 127 250 685 993 191
Cuts at: 3867 33
Size: 1121
Fragments arranged by size:
1410 1121 993 685 250 191 171 127
7
SfiI GGCCn_nnn'nGGCC
Cuts at: 857 857
Size: 4955
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 988 1481 1977 988
Size: 493 496 3966
Fragments arranged by size:
3966 496 493
SmlI C'TyrA_G
Cuts at: 704 1522 1876 2110 3025 3517 3779 704
Size: 818 354 234 915 492 262 1880
Fragments arranged by size:
1880 915 818 492 354 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 2008 2508 2008
Size: 500 4455
SrfI GCCC'GGGC
Cuts at: 988 988
Size: 4955
StyI C'CwwG_G
Cuts at: 243 425 854 1142 1637 1844 2089 2443 243
Size: 182 429 288 495 207 245 354 2755
Fragments arranged by size:
2755 495 429 354 288 245 207 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 339 843 847 1294 2538 2671 3130 3339
Size: 35 504 4 447 1244 133 459 209
Cuts at: 3339 304
Size: 1920
Fragments arranged by size:
1920 1244 504 459 447 209 133 35
4
TatI w'GTAC_w
Cuts at: 602 4470 602
Size: 3868 1087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1200 1232 4472 4504 1200
Size: 32 3240 32 1651
Fragments arranged by size:
3240 1651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1510 2752 1510
Size: 1242 3713
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2450 114
Size: 2336 2619
XhoI C'TCGA_G
Cuts at: 704 704
Size: 4955
XmaI C'CCGG_G
Cuts at: 986 1479 1975 986
Size: 493 496 3966
Fragments arranged by size:
3966 496 493
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PflMI PpuMI
PshAI PsiI PspXI SanDI SexAI SgrAI SnaBI SspI
StuI SwaI XmnI ZraI