Restriction map for
pF1KB7822
(Circular) (Six-base) MAPSORT of: pF1KB7822.seq Check: 5583 from: 1 to: 4913
pF1KB7822 4913 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1323 1323
Size: 4913
AatII G_ACGT'C
Cuts at: 287 287
Size: 4913
AccI GT'mk_AC
Cuts at: 1951 1951
Size: 4913
Acc65I G'GTAC_C
Cuts at: 1295 1929 1295
Size: 634 4279
AclI AA'CG_TT
Cuts at: 4753 4753
Size: 4913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1672 2738 3170 3917 4185 508
Size: 1164 1066 432 747 268 1236
Fragments arranged by size:
1236 1164 1066 747 432 268
AflIII A'CryG_T
Cuts at: 2265 3369 4346 2265
Size: 1104 977 2832
Fragments arranged by size:
2832 1104 977
AgeI A'CCGG_T
Cuts at: 3301 3301
Size: 4913
AleI CACnn'nnGTG
Cuts at: 1039 1039
Size: 4913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 453 453
Size: 4913
AlwNI CAG_nnn'CTG
Cuts at: 513 528 864 1158 2218 3785 513
Size: 15 336 294 1060 1567 1641
Fragments arranged by size:
1641 1567 1060 336 294 15
ApaLI G'TGCA_C
Cuts at: 1453 3683 1453
Size: 2230 2683
ApoI r'AATT_y
Cuts at: 1917 4266 4522 1917
Size: 2349 256 2308
Fragments arranged by size:
2349 2308 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4913
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
AvaI C'yCGr_G
Cuts at: 517 804 1093 1211 1304 1739 1933 517
Size: 287 289 118 93 435 194 3497
Fragments arranged by size:
3497 435 289 287 194 118 93
BamHI G'GATC_C
Cuts at: 1938 1938
Size: 4913
BanI G'GyrC_C
Cuts at: 1041 1295 1701 1929 2343 2591 2626 1041
Size: 254 406 228 414 248 35 3328
Fragments arranged by size:
3328 414 406 254 248 228 35
BanII G_rGCy'C
Cuts at: 314 955 994 1202 1273 1519 1629 1927 2957
Size: 641 39 208 71 246 110 298 1030
Cuts at: 2957 314
Size: 2270
Fragments arranged by size:
2270 1030 641 298 246 208 110 71
39
BbeI G_GCGC'C
Cuts at: 2347 2595 2347
Size: 248 4665
BbvCI CC'TCA_GC
Cuts at: 1340 1340
Size: 4913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1833 1867 1833
Size: 34 4879
BciVI GTATCCnnnnn_n'
Cuts at: 2811 3572 4913 2811
Size: 761 1341 2811
Fragments arranged by size:
2811 1341 761
BclI T'GATC_A
Cuts at: 2433 2433
Size: 4913
BglI GCCn_nnn'nGGC
Cuts at: 322 722 322
Size: 400 4513
BglII A'GATC_T
Cuts at: 2428 4465 2428
Size: 2037 2876
BlpI GC'TnA_GC
Cuts at: 2025 2025
Size: 4913
Bme1580I G_kGCm'C
Cuts at: 1001 1457 2538 2631 3687 1001
Size: 456 1081 93 1056 2227
Fragments arranged by size:
2227 1081 1056 456 93
BmrI ACTGGGnnnn_n'
Cuts at: 1100 1114 2125 2206 2541 4685 1100
Size: 14 1011 81 335 2144 1328
Fragments arranged by size:
2144 1328 1011 335 81 14
BmtI G_CTAG'C
Cuts at: 614 614
Size: 4913
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 4913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 241 490 754 1495 198
Size: 43 249 264 741 3616
Fragments arranged by size:
3616 741 264 249 43
Bpu10I CC'TnA_GC
Cuts at: 1340 1576 1340
Size: 236 4677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1153 2089 2968 3460 3758 271
Size: 882 936 879 492 298 1426
Fragments arranged by size:
1426 936 882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 606 4617 606
Size: 4011 902
BsaAI yAC'GTr
Cuts at: 2266 2266
Size: 4913
BsaBI GATnn'nnATC
Cuts at: 6 2427 4464 6
Size: 2421 2037 455
Fragments arranged by size:
2421 2037 455
BsaHI Gr'CG_yC
Cuts at: 124 284 1349 2344 2592 3294 4790 124
Size: 160 1065 995 248 702 1496 247
Fragments arranged by size:
1496 1065 995 702 248 247 160
BsaWI w'CCGG_w
Cuts at: 182 1361 2102 2623 3301 3575 3722 4133 182
Size: 1179 741 521 678 274 147 411 962
Fragments arranged by size:
1179 962 741 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 102 132 784 814 1453 1483 102
Size: 30 652 30 639 30 3532
Fragments arranged by size:
3532 652 639 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 1224 1251 1675 322
Size: 902 27 424 3560
Fragments arranged by size:
3560 902 424 27
BseYI C'CCAG_C
Cuts at: 537 1000 1191 2338 3096 3673 4169 537
Size: 463 191 1147 758 577 496 1281
Fragments arranged by size:
1281 1147 758 577 496 463 191
BsiEI CG_ry'CG
Cuts at: 104 445 790 2455 2501 3709 104
Size: 341 345 1665 46 1208 1308
Fragments arranged by size:
1665 1308 1208 345 341 46
BsiHKAI G_wGCw'C
Cuts at: 314 1457 1669 1927 2705 2895 3687 314
Size: 1143 212 258 778 190 792 1540
Fragments arranged by size:
1540 1143 792 778 258 212 190
BsiWI C'GTAC_G
Cuts at: 1083 1083
Size: 4913
BsmBI CGTCTCn'nnnn_
Cuts at: 131 131
Size: 4913
Bsp1286I G_dGCh'C
Cuts at: 314 955 994 1001 1202 1273 1457 1519 1629
Size: 641 39 7 201 71 184 62 110
Cuts at: 1629 1669 1927 2538 2631 2705 2895 2957 3687
Size: 40 258 611 93 74 190 62 730
Cuts at: 3687 314
Size: 1540
Fragments arranged by size:
1540 730 641 611 258 201 190 184
110 93 74 71 62 62 40 39
7
BspEI T'CCGG_A
Cuts at: 182 4133 182
Size: 3951 962
BspHI T'CATG_A
Cuts at: 4089 4089
Size: 4913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1323 1963 2177 2479 2860 1323
Size: 640 214 302 381 3376
Fragments arranged by size:
3376 640 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1374 3205 3259 4743 4908 50
Size: 1324 1831 54 1484 165 55
Fragments arranged by size:
1831 1484 1324 165 55 54
BsrDI GCAATG_nn'
Cuts at: 504 2825 504
Size: 2321 2592
BsrFI r'CCGG_y
Cuts at: 146 363 409 440 1580 2911 3092 3301 146
Size: 217 46 31 1140 1331 181 209 1758
Fragments arranged by size:
1758 1331 1140 217 209 181 46 31
BssHII G'CGCG_C
Cuts at: 1610 1610
Size: 4913
BssSI C'ACGA_G
Cuts at: 3184 3542 3184
Size: 358 4555
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 2290 1030
Size: 1260 3653
BstBI TT'CG_AA
Cuts at: 1523 3274 1523
Size: 1751 3162
BstEII G'GTnAC_C
Cuts at: 559 559
Size: 4913
BstXI CCAn_nnnn'nTGG
Cuts at: 693 1127 693
Size: 434 4479
BstYI r'GATC_y
Cuts at: 1 767 1938 2405 2428 2763 3009 4021 4107
Size: 766 1171 467 23 335 246 1012 86
Cuts at: 4107 4119 4465 1
Size: 12 346 449
Fragments arranged by size:
1171 1012 766 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1263 1263
Size: 4913
BtgI C'CryG_G
Cuts at: 1012 1718 1012
Size: 706 4207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2863 3044 3207 2863
Size: 181 163 4569
Fragments arranged by size:
4569 181 163
BtsI GCAGTG_nn'
Cuts at: 708 1846 2156 708
Size: 1138 310 3465
Fragments arranged by size:
3465 1138 310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 741 776 741
Size: 35 4878
DraI TTT'AAA
Cuts at: 1912 1912
Size: 4913
DrdI GACnn_nn'nnGTC
Cuts at: 625 2619 3477 625
Size: 1994 858 2061
Fragments arranged by size:
2061 1994 858
EaeI y'GGCC_r
Cuts at: 442 576 1786 2498 2672 3063 3090 4435 442
Size: 134 1210 712 174 391 27 1345 920
Fragments arranged by size:
1345 1210 920 712 391 174 134 27
EagI C'GGCC_G
Cuts at: 442 2498 442
Size: 2056 2857
EarI CTCTTCn'nnn_
Cuts at: 484 1285 2936 3146 484
Size: 801 1651 210 2251
Fragments arranged by size:
2251 1651 801 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1258 3121 3431 3577 4723 1258
Size: 1863 310 146 1146 1448
Fragments arranged by size:
1863 1448 1146 310 146
EcoICRI GAG'CTC
Cuts at: 312 1925 312
Size: 1613 3300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 241 490 508 754 1495 1672 2738 3170
Size: 43 249 18 246 741 177 1066 432
Cuts at: 3170 3917 4185 198
Size: 747 268 926
Fragments arranged by size:
1066 926 747 741 432 268 249 246
177 43 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1654 1654
Size: 4913
EcoO109I rG'GnC_Cy
Cuts at: 889 907 2052 889
Size: 18 1145 3750
Fragments arranged by size:
3750 1145 18
EcoRI G'AATT_C
Cuts at: 1917 1917
Size: 4913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 265 1401 2932 265
Size: 1136 1531 2246
Fragments arranged by size:
2246 1531 1136
FspI TGC'GCA
Cuts at: 2694 2694
Size: 4913
HaeII r_GCGC'y
Cuts at: 388 555 1878 2347 2595 3617 4596 388
Size: 167 1323 469 248 1022 979 705
Fragments arranged by size:
1323 1022 979 705 469 248 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 1243 1275 1398 1430 1453 1485 2755
Size: 32 1109 32 123 32 23 32 1270
Cuts at: 2755 2785 2787 2817 102
Size: 30 2 30 2198
Fragments arranged by size:
2198 1270 1109 123 32 32 32 32
30 30 23 2
HincII GTy'rAC
Cuts at: 777 1320 1952 777
Size: 543 632 3738
Fragments arranged by size:
3738 632 543
HpaI GTT'AAC
Cuts at: 1320 1320
Size: 4913
KasI G'GCGC_C
Cuts at: 2343 2591 2343
Size: 248 4665
KpnI G_GTAC'C
Cuts at: 1299 1933 1299
Size: 634 4279
MluI A'CGCG_T
Cuts at: 4346 4346
Size: 4913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3584 3768 3584
Size: 184 4729
MscI TGG'CCA
Cuts at: 2674 2674
Size: 4913
MslI CAynn'nnrTG
Cuts at: 374 1039 2902 3029 374
Size: 665 1863 127 2258
Fragments arranged by size:
2258 1863 665 127
MspA1I CmG'CkG
Cuts at: 115 525 864 1014 1155 1689 2024 2338 2698
Size: 410 339 150 141 534 335 314 360
Cuts at: 2698 3711 3956 115
Size: 1013 245 1072
Fragments arranged by size:
1072 1013 534 410 360 339 335 314
245 150 141
NaeI GCC'GGC
Cuts at: 365 442 1582 3094 365
Size: 77 1140 1512 2184
Fragments arranged by size:
2184 1512 1140 77
NarI GG'CG_CC
Cuts at: 2344 2592 2344
Size: 248 4665
NgoMIV G'CCGG_C
Cuts at: 363 440 1580 3092 363
Size: 77 1140 1512 2184
Fragments arranged by size:
2184 1512 1140 77
NheI G'CTAG_C
Cuts at: 610 610
Size: 4913
NspI r_CATG'y
Cuts at: 712 1696 1966 2466 3373 712
Size: 984 270 500 907 2252
Fragments arranged by size:
2252 984 907 500 270
PasI CC'CwG_GG
Cuts at: 583 1621 583
Size: 1038 3875
PciI A'CATG_T
Cuts at: 3369 3369
Size: 4913
PflMI CCAn_nnn'nTGG
Cuts at: 1158 1784 1158
Size: 626 4287
PfoI T'CCnGG_A
Cuts at: 1471 1471
Size: 4913
PmeI GTTT'AAAC
Cuts at: 1912 1912
Size: 4913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4881
PpuMI rG'GwC_Cy
Cuts at: 907 907
Size: 4913
PspXI vC'TCGA_Gb
Cuts at: 1093 1093
Size: 4913
PstI C_TGCA'G
Cuts at: 158 860 1960 2645 158
Size: 702 1100 685 2426
Fragments arranged by size:
2426 1100 702 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4913
PvuII CAG'CTG
Cuts at: 864 1689 2338 2698 864
Size: 825 649 360 3079
Fragments arranged by size:
3079 825 649 360
RsrII CG'GwC_CG
Cuts at: 3108 3108
Size: 4913
SacI G_AGCT'C
Cuts at: 314 1927 314
Size: 1613 3300
SacII CC_GC'GG
Cuts at: 1015 1015
Size: 4913
SalI G'TCGA_C
Cuts at: 1950 1950
Size: 4913
SanDI GG'GwC_CC
Cuts at: 907 907
Size: 4913
SapI GCTCTTCn'nnn_
Cuts at: 484 2936 3146 484
Size: 2452 210 2251
Fragments arranged by size:
2452 2251 210
SbfI CC_TGCA'GG
Cuts at: 1960 1960
Size: 4913
ScaI AGT'ACT
Cuts at: 4430 4430
Size: 4913
SfcI C'TryA_G
Cuts at: 33 154 856 1956 2641 3634 3825 33
Size: 121 702 1100 685 993 191 1121
Fragments arranged by size:
1121 1100 993 702 685 191 121
SfoI GGC'GCC
Cuts at: 2345 2593 2345
Size: 248 4665
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
SmaI CCC'GGG
Cuts at: 519 806 1213 1741 1935 519
Size: 287 407 528 194 3497
Fragments arranged by size:
3497 528 407 287 194
SmlI C'TyrA_G
Cuts at: 250 1093 1132 1304 2068 2983 3475 3737 250
Size: 843 39 172 764 915 492 262 1426
Fragments arranged by size:
1426 915 843 764 492 262 172 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4913
SphI G_CATG'C
Cuts at: 712 1966 2466 712
Size: 1254 500 3159
Fragments arranged by size:
3159 1254 500
SrfI GCCC'GGGC
Cuts at: 519 519
Size: 4913
StyI C'CwwG_G
Cuts at: 596 902 986 1238 1499 2047 2401 596
Size: 306 84 252 261 548 354 3108
Fragments arranged by size:
3108 548 354 306 261 252 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 483 596 609 999 2496 2629 3088 3297 483
Size: 113 13 390 1497 133 459 209 2099
Fragments arranged by size:
2099 1497 459 390 209 133 113 13
TatI w'GTAC_w
Cuts at: 4428 4428
Size: 4913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 784 816 4430 4462 784
Size: 32 3614 32 1235
Fragments arranged by size:
3614 1235 32 32
Tth111I GACn'n_nGTC
Cuts at: 2710 2710
Size: 4913
XbaI T'CTAG_A
Cuts at: 1944 1944
Size: 4913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1479 2408 1479
Size: 929 3984
XhoI C'TCGA_G
Cuts at: 1093 1304 1093
Size: 211 4702
XmaI C'CCGG_G
Cuts at: 517 804 1211 1739 1933 517
Size: 287 407 528 194 3497
Fragments arranged by size:
3497 528 407 287 194
ZraI GAC'GTC
Cuts at: 285 285
Size: 4913
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BfrBI BmgBI BsgI BsmI BsrGI BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII MfeI NcoI NdeI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PsrI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XmnI