Restriction map for
pF1KB7815
(Circular) (Six-base) MAPSORT of: pF1KB7815.seq Check: 1070 from: 1 to: 4709
pF1KB7815 4709 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1747 1747
Size: 4709
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 4549 4549
Size: 4709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2966 3713 3981 2534
Size: 432 747 268 3262
Fragments arranged by size:
3262 747 432 268
AfeI AGC'GCT
Cuts at: 376 376
Size: 4709
AflIII A'CryG_T
Cuts at: 1099 2061 3165 4142 1099
Size: 962 1104 977 1666
Fragments arranged by size:
1666 1104 977 962
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AleI CACnn'nnGTG
Cuts at: 1448 1448
Size: 4709
AlwNI CAG_nnn'CTG
Cuts at: 468 2014 3581 468
Size: 1546 1567 1596
Fragments arranged by size:
1596 1567 1546
ApaLI G'TGCA_C
Cuts at: 3479 3479
Size: 4709
ApoI r'AATT_y
Cuts at: 637 1037 1205 1713 4062 4318 637
Size: 400 168 508 2349 256 1028
Fragments arranged by size:
2349 1028 508 400 256 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 1729 1729
Size: 4709
BamHI G'GATC_C
Cuts at: 1734 1734
Size: 4709
BanI G'GyrC_C
Cuts at: 1725 2139 2387 2422 1725
Size: 414 248 35 4012
Fragments arranged by size:
4012 414 248 35
BanII G_rGCy'C
Cuts at: 1723 2753 1723
Size: 1030 3679
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 1102 1102
Size: 4709
BciVI GTATCCnnnnn_n'
Cuts at: 2607 3368 4709 2607
Size: 761 1341 2607
Fragments arranged by size:
2607 1341 761
BclI T'GATC_A
Cuts at: 360 774 1667 2229 360
Size: 414 893 562 2840
Fragments arranged by size:
2840 893 562 414
BfrBI ATG'CAT
Cuts at: 430 789 430
Size: 359 4350
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 1821 1821
Size: 4709
Bme1580I G_kGCm'C
Cuts at: 2334 2427 3483 2334
Size: 93 1056 3560
Fragments arranged by size:
3560 1056 93
BmgBI CAC'GTC
Cuts at: 1100 1100
Size: 4709
BmrI ACTGGGnnnn_n'
Cuts at: 1696 1921 2002 2337 4481 1696
Size: 225 81 335 2144 1924
Fragments arranged by size:
2144 1924 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1488 3076 1488
Size: 1588 3121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 391 822 157
Size: 234 431 4044
Fragments arranged by size:
4044 431 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1885 2764 3256 3554 907
Size: 978 879 492 298 2062
Fragments arranged by size:
2062 978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1192 1299 4413 1192
Size: 107 3114 1488
Fragments arranged by size:
3114 1488 107
BsaAI yAC'GTr
Cuts at: 502 2062 502
Size: 1560 3149
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 1138 2140 2388 3090 4586 1138
Size: 1002 248 702 1496 1261
Fragments arranged by size:
1496 1261 1002 702 248
BsaWI w'CCGG_w
Cuts at: 267 961 1898 2419 3097 3371 3518 3929 267
Size: 694 937 521 678 274 147 411 1047
Fragments arranged by size:
1047 937 694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 1531 181
Size: 1350 3359
BseYI C'CCAG_C
Cuts at: 2134 2892 3469 3965 2134
Size: 758 577 496 2878
Fragments arranged by size:
2878 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 1170 1610 750
Size: 420 440 3849
Fragments arranged by size:
3849 440 420
BsiEI CG_ry'CG
Cuts at: 104 2251 2297 3505 104
Size: 2147 46 1208 1308
Fragments arranged by size:
2147 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1723 2501 2691 3483 1723
Size: 778 190 792 2949
Fragments arranged by size:
2949 792 778 190
BsmI GAATG_Cn'
Cuts at: 901 1644 901
Size: 743 3966
Bsp1286I G_dGCh'C
Cuts at: 1723 2334 2427 2501 2691 2753 3483 1723
Size: 611 93 74 190 62 730 2949
Fragments arranged by size:
2949 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3929 3929
Size: 4709
BspHI T'CATG_A
Cuts at: 417 483 3885 417
Size: 66 3402 1241
Fragments arranged by size:
3402 1241 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1759 1973 2275 2656 1759
Size: 214 302 381 3812
Fragments arranged by size:
3812 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 447 1074 2621 447
Size: 627 1547 2535
Fragments arranged by size:
2535 1547 627
BsrFI r'CCGG_y
Cuts at: 2707 2888 3097 2707
Size: 181 209 4319
Fragments arranged by size:
4319 209 181
BsrGI T'GTAC_A
Cuts at: 1026 1026
Size: 4709
BssSI C'ACGA_G
Cuts at: 2980 3338 2980
Size: 358 4351
BstAPI GCAn_nnn'nTGC
Cuts at: 2086 2086
Size: 4709
BstBI TT'CG_AA
Cuts at: 3070 3070
Size: 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 4709
BstYI r'GATC_y
Cuts at: 1 1734 2201 2224 2559 2805 3817 3903 3915
Size: 1733 467 23 335 246 1012 86 12
Cuts at: 3915 4261 1
Size: 346 449
Fragments arranged by size:
1733 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1596 1596
Size: 4709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2659 2840 3003 2659
Size: 181 163 4365
Fragments arranged by size:
4365 181 163
BtsI GCAGTG_nn'
Cuts at: 251 738 990 1158 1649 1952 251
Size: 487 252 168 491 303 3008
Fragments arranged by size:
3008 491 487 303 252 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1056 1091 1056
Size: 35 4674
DraI TTT'AAA
Cuts at: 1708 1708
Size: 4709
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3273 2415
Size: 858 3851
EaeI y'GGCC_r
Cuts at: 2294 2468 2859 2886 4231 2294
Size: 174 391 27 1345 2772
Fragments arranged by size:
2772 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 283 2732 2942 283
Size: 2449 210 2050
Fragments arranged by size:
2449 2050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2917 3227 3373 4519 2917
Size: 310 146 1146 3107
Fragments arranged by size:
3107 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1721 1721
Size: 4709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 391 822 2534 2966 3713 3981 157
Size: 234 431 1712 432 747 268 885
Fragments arranged by size:
1712 885 747 432 431 268 234
EcoO109I rG'GnC_Cy
Cuts at: 198 1848 198
Size: 1650 3059
EcoRI G'AATT_C
Cuts at: 1037 1205 1713 1037
Size: 168 508 4033
Fragments arranged by size:
4033 508 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1158 2728 1158
Size: 1570 3139
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 188 378 2143 2391 3413 4392 188
Size: 190 1765 248 1022 979 505
Fragments arranged by size:
1765 1022 979 505 248 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2551 2581 2583 2613 2551
Size: 30 2 30 4647
Fragments arranged by size:
4647 30 30 2
HincII GTy'rAC
Cuts at: 537 1748 537
Size: 1211 3498
HpaI GTT'AAC
Cuts at: 537 537
Size: 4709
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MfeI C'AATT_G
Cuts at: 311 311
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 266 3380 3564 200
Size: 66 3114 184 1345
Fragments arranged by size:
3114 1345 184 66
MscI TGG'CCA
Cuts at: 2470 2470
Size: 4709
MslI CAynn'nnrTG
Cuts at: 422 1046 1448 2698 2825 422
Size: 624 402 1250 127 2306
Fragments arranged by size:
2306 1250 624 402 127
MspA1I CmG'CkG
Cuts at: 1535 1820 2134 2494 3507 3752 1535
Size: 285 314 360 1013 245 2492
Fragments arranged by size:
2492 1013 360 314 285 245
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NdeI CA'TA_TG
Cuts at: 349 349
Size: 4709
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NsiI A_TGCA'T
Cuts at: 432 791 432
Size: 359 4350
NspI r_CATG'y
Cuts at: 1762 2262 3169 1762
Size: 500 907 3302
Fragments arranged by size:
3302 907 500
PciI A'CATG_T
Cuts at: 3165 3165
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4677
PpuMI rG'GwC_Cy
Cuts at: 198 198
Size: 4709
PstI C_TGCA'G
Cuts at: 1425 1756 2441 1425
Size: 331 685 3693
Fragments arranged by size:
3693 685 331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 2134 2494 2134
Size: 360 4349
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 1723 1723
Size: 4709
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SexAI A'CCwGG_T
Cuts at: 236 779 236
Size: 543 4166
SfcI C'TryA_G
Cuts at: 33 228 1421 1752 2437 3430 3621 33
Size: 195 1193 331 685 993 191 1121
Fragments arranged by size:
1193 1121 993 685 331 195 191
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1731 1731
Size: 4709
SmlI C'TyrA_G
Cuts at: 922 1864 2779 3271 3533 922
Size: 942 915 492 262 2098
Fragments arranged by size:
2098 942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1762 2262 1762
Size: 500 4209
SspI AAT'ATT
Cuts at: 441 441
Size: 4709
StyI C'CwwG_G
Cuts at: 1403 1843 2197 1403
Size: 440 354 3915
Fragments arranged by size:
3915 440 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2292 2425 2884 3093 2292
Size: 133 459 209 3908
Fragments arranged by size:
3908 459 209 133
TatI w'GTAC_w
Cuts at: 1026 4224 1026
Size: 3198 1511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1562 1594 4226 4258 1562
Size: 32 2632 32 2013
Fragments arranged by size:
2632 2013 32 32
Tth111I GACn'n_nGTC
Cuts at: 2506 2506
Size: 4709
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 4709
XmaI C'CCGG_G
Cuts at: 1729 1729
Size: 4709
XmnI GAAnn'nnTTC
Cuts at: 620 748 832 620
Size: 128 84 4497
Fragments arranged by size:
4497 128 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI
BsiWI BsmBI BssHII BstEII BstZ17I BtgI ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII NcoI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI