Restriction map for
pF1KB7807
(Circular) (Six-base) MAPSORT of: pF1KB7807.seq Check: 6586 from: 1 to: 4688
pF1KB7807 4688 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 778 778
Size: 4688
AccI GT'mk_AC
Cuts at: 1726 1726
Size: 4688
Acc65I G'GTAC_C
Cuts at: 511 1704 511
Size: 1193 3495
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 1229 1252 2513 2945 3692 3960 429
Size: 800 23 1261 432 747 268 1157
Fragments arranged by size:
1261 1157 800 747 432 268 23
AflIII A'CryG_T
Cuts at: 2040 3144 4121 2040
Size: 1104 977 2607
Fragments arranged by size:
2607 1104 977
AgeI A'CCGG_T
Cuts at: 3076 3076
Size: 4688
AlwNI CAG_nnn'CTG
Cuts at: 321 460 1297 1482 1547 1993 3560 321
Size: 139 837 185 65 446 1567 1449
Fragments arranged by size:
1567 1449 837 446 185 139 65
ApaLI G'TGCA_C
Cuts at: 3458 3458
Size: 4688
ApoI r'AATT_y
Cuts at: 1692 4041 4297 1692
Size: 2349 256 2083
Fragments arranged by size:
2349 2083 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4688
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 200 1160 1708 200
Size: 960 548 3180
Fragments arranged by size:
3180 960 548
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4688
BanI G'GyrC_C
Cuts at: 398 511 1704 2118 2366 2401 398
Size: 113 1193 414 248 35 2685
Fragments arranged by size:
2685 1193 414 248 113 35
BanII G_rGCy'C
Cuts at: 443 595 1067 1136 1195 1702 2732 443
Size: 152 472 69 59 507 1030 2399
Fragments arranged by size:
2399 1030 507 472 152 69 59
BbeI G_GCGC'C
Cuts at: 402 2122 2370 402
Size: 1720 248 2720
Fragments arranged by size:
2720 1720 248
BbvCI CC'TCA_GC
Cuts at: 1540 1667 1540
Size: 127 4561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1066 1100 90
Size: 34 942 34 3678
Fragments arranged by size:
3678 942 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3347 4688 2586
Size: 761 1341 2586
Fragments arranged by size:
2586 1341 761
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglII A'GATC_T
Cuts at: 494 2203 4240 494
Size: 1709 2037 942
Fragments arranged by size:
2037 1709 942
BlpI GC'TnA_GC
Cuts at: 1051 1800 1051
Size: 749 3939
Bme1580I G_kGCm'C
Cuts at: 201 573 947 2313 2406 3462 201
Size: 372 374 1366 93 1056 1427
Fragments arranged by size:
1427 1366 1056 374 372 93
BmgBI CAC'GTC
Cuts at: 312 312
Size: 4688
BmrI ACTGGGnnnn_n'
Cuts at: 188 776 806 1083 1225 1900 1981 2316 4460
Size: 588 30 277 142 675 81 335 2144
Cuts at: 4460 188
Size: 416
Fragments arranged by size:
2144 675 588 416 335 277 142 81
30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 735 1150 1155 3055 735
Size: 415 5 1900 2368
Fragments arranged by size:
2368 1900 415 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 459 662 838 1179 1351 370
Size: 89 203 176 341 172 3707
Fragments arranged by size:
3707 341 203 176 172 89
Bpu10I CC'TnA_GC
Cuts at: 1540 1667 1540
Size: 127 4561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1864 2743 3235 3533 172
Size: 1692 879 492 298 1327
Fragments arranged by size:
1692 1327 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 4392 277
Size: 4115 573
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 399 1408 2119 2367 3069 4565 399
Size: 1009 711 248 702 1496 522
Fragments arranged by size:
1496 1009 711 702 522 248
BsaWI w'CCGG_w
Cuts at: 866 1877 2398 3076 3350 3497 3908 866
Size: 1011 521 678 274 147 411 1646
Fragments arranged by size:
1646 1011 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 1165
Size: 30 4658
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 355 716 724 764 1000 1348 352
Size: 3 361 8 40 236 348 3692
Fragments arranged by size:
3692 361 348 236 40 8 3
BseYI C'CCAG_C
Cuts at: 225 772 889 1324 2113 2871 3448 3944 225
Size: 547 117 435 789 758 577 496 969
Fragments arranged by size:
969 789 758 577 547 496 435 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1042 334
Size: 708 3980
BsiEI CG_ry'CG
Cuts at: 104 1527 2230 2276 3484 104
Size: 1423 703 46 1208 1308
Fragments arranged by size:
1423 1308 1208 703 46
BsiHKAI G_wGCw'C
Cuts at: 676 1067 1136 1171 1702 2480 2670 3462 676
Size: 391 69 35 531 778 190 792 1902
Fragments arranged by size:
1902 792 778 531 391 190 69 35
BsmBI CGTCTCn'nnnn_
Cuts at: 477 1521 477
Size: 1044 3644
Bsp1286I G_dGCh'C
Cuts at: 201 443 573 595 676 947 1067 1136 1171
Size: 242 130 22 81 271 120 69 35
Cuts at: 1171 1195 1702 2313 2406 2480 2670 2732 3462
Size: 24 507 611 93 74 190 62 730
Cuts at: 3462 201
Size: 1427
Fragments arranged by size:
1427 730 611 507 271 242 190 130
120 93 81 74 69 62 35 24
22
BspEI T'CCGG_A
Cuts at: 866 3908 866
Size: 3042 1646
BspHI T'CATG_A
Cuts at: 3864 3864
Size: 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 1433 1671 1738 1952 2254 2635 778
Size: 655 238 67 214 302 381 2831
Fragments arranged by size:
2831 655 381 302 238 214 67
BsrBI CCG'CTC
Cuts at: 50 486 2980 3034 4518 4683 50
Size: 436 2494 54 1484 165 55
Fragments arranged by size:
2494 1484 436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 540 1435 2600 540
Size: 895 1165 2628
Fragments arranged by size:
2628 1165 895
BsrFI r'CCGG_y
Cuts at: 1676 2686 2867 3076 1676
Size: 1010 181 209 3288
Fragments arranged by size:
3288 1010 209 181
BssHII G'CGCG_C
Cuts at: 258 258
Size: 4688
BssSI C'ACGA_G
Cuts at: 1060 2959 3317 1060
Size: 1899 358 2431
Fragments arranged by size:
2431 1899 358
BstAPI GCAn_nnn'nTGC
Cuts at: 568 2065 568
Size: 1497 3191
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstYI r'GATC_y
Cuts at: 1 494 656 860 989 1713 2180 2203 2538
Size: 493 162 204 129 724 467 23 335
Cuts at: 2538 2784 3796 3882 3894 4240 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 724 493 467 449 346 335 246
204 162 129 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 758 758
Size: 4688
BtgI C'CryG_G
Cuts at: 206 1446 206
Size: 1240 3448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 211 573 622 1931 211
Size: 362 49 1309 2968
Fragments arranged by size:
2968 1309 362 49
DraI TTT'AAA
Cuts at: 1687 1687
Size: 4688
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3252 2394
Size: 858 3830
EaeI y'GGCC_r
Cuts at: 374 661 1014 2273 2447 2838 2865 4210 374
Size: 287 353 1259 174 391 27 1345 852
Fragments arranged by size:
1345 1259 852 391 353 287 174 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4688
EarI CTCTTCn'nnn_
Cuts at: 448 493 701 977 1141 2711 2921 448
Size: 45 208 276 164 1570 210 2215
Fragments arranged by size:
2215 1570 276 210 208 164 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 164 2896 3206 3352 4498 164
Size: 2732 310 146 1146 354
Fragments arranged by size:
2732 1146 354 310 146
EcoICRI GAG'CTC
Cuts at: 1065 1134 1700 1065
Size: 69 566 4053
Fragments arranged by size:
4053 566 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 429 459 662 838 1179 1229 1252 1351
Size: 59 30 203 176 341 50 23 99
Cuts at: 1351 2513 2945 3692 3960 370
Size: 1162 432 747 268 1098
Fragments arranged by size:
1162 1098 747 432 341 268 203 176
99 59 50 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 406 641 1289 1333 1610 406
Size: 235 648 44 277 3484
Fragments arranged by size:
3484 648 277 235 44
EcoO109I rG'GnC_Cy
Cuts at: 249 634 648 1222 1294 1615 1827 249
Size: 385 14 574 72 321 212 3110
Fragments arranged by size:
3110 574 385 321 212 72 14
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4688
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4688
HaeII r_GCGC'y
Cuts at: 121 164 402 1346 2122 2370 3392 4371 121
Size: 43 238 944 776 248 1022 979 438
Fragments arranged by size:
1022 979 944 776 438 248 238 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 886 918 2530 2560 2562 2592 601
Size: 32 253 32 1612 30 2 30 2697
Fragments arranged by size:
2697 1612 253 32 32 30 30 2
HincII GTy'rAC
Cuts at: 451 1727 451
Size: 1276 3412
KasI G'GCGC_C
Cuts at: 398 2118 2366 398
Size: 1720 248 2720
Fragments arranged by size:
2720 1720 248
KpnI G_GTAC'C
Cuts at: 515 1708 515
Size: 1193 3495
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 665 1233 3359 3543 665
Size: 568 2126 184 1810
Fragments arranged by size:
2126 1810 568 184
MscI TGG'CCA
Cuts at: 376 663 2449 376
Size: 287 1786 2615
Fragments arranged by size:
2615 1786 287
MslI CAynn'nnrTG
Cuts at: 841 2677 2804 841
Size: 1836 127 2725
Fragments arranged by size:
2725 1836 127
MspA1I CmG'CkG
Cuts at: 240 321 1799 2113 2473 3486 3731 240
Size: 81 1478 314 360 1013 245 1197
Fragments arranged by size:
1478 1197 1013 360 314 245 81
NaeI GCC'GGC
Cuts at: 1678 2869 1678
Size: 1191 3497
NarI GG'CG_CC
Cuts at: 399 2119 2367 399
Size: 1720 248 2720
Fragments arranged by size:
2720 1720 248
NcoI C'CATG_G
Cuts at: 206 1446 206
Size: 1240 3448
NgoMIV G'CCGG_C
Cuts at: 1676 2867 1676
Size: 1191 3497
NspI r_CATG'y
Cuts at: 1741 2241 3148 1741
Size: 500 907 3281
Fragments arranged by size:
3281 907 500
PasI CC'CwG_GG
Cuts at: 550 550
Size: 4688
PciI A'CATG_T
Cuts at: 3144 3144
Size: 4688
PflMI CCAn_nnn'nTGG
Cuts at: 628 1447 628
Size: 819 3869
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4656
PpuMI rG'GwC_Cy
Cuts at: 249 634 648 1615 249
Size: 385 14 967 3322
Fragments arranged by size:
3322 967 385 14
PshAI GACnn'nnGTC
Cuts at: 1601 1601
Size: 4688
PstI C_TGCA'G
Cuts at: 179 239 257 407 744 1475 1735 2420 179
Size: 60 18 150 337 731 260 685 2447
Fragments arranged by size:
2447 731 685 337 260 150 60 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 240 321 2113 2473 240
Size: 81 1792 360 2455
Fragments arranged by size:
2455 1792 360 81
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1067 1136 1702 1067
Size: 69 566 4053
Fragments arranged by size:
4053 566 69
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SanDI GG'GwC_CC
Cuts at: 648 648
Size: 4688
SapI GCTCTTCn'nnn_
Cuts at: 448 493 1141 2711 2921 448
Size: 45 648 1570 210 2215
Fragments arranged by size:
2215 1570 648 210 45
SbfI CC_TGCA'GG
Cuts at: 744 1735 744
Size: 991 3697
ScaI AGT'ACT
Cuts at: 4205 4205
Size: 4688
SfcI C'TryA_G
Cuts at: 33 175 235 253 403 740 1471 1731 2416
Size: 142 60 18 150 337 731 260 685
Cuts at: 2416 3409 3600 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 731 685 337 260 191 150
142 60 18
SfoI GGC'GCC
Cuts at: 400 2120 2368 400
Size: 1720 248 2720
Fragments arranged by size:
2720 1720 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 187 1843 2758 3250 3512 187
Size: 1656 915 492 262 1363
Fragments arranged by size:
1656 1363 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
StyI C'CwwG_G
Cuts at: 206 877 1399 1446 1822 2176 206
Size: 671 522 47 376 354 2718
Fragments arranged by size:
2718 671 522 376 354 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 827 2271 2404 2863 3072 827
Size: 1444 133 459 209 2443
Fragments arranged by size:
2443 1444 459 209 133
TatI w'GTAC_w
Cuts at: 4203 4203
Size: 4688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 786 818 4205 4237 786
Size: 32 3387 32 1237
Fragments arranged by size:
3387 1237 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4688
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2183
Size: 4688
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BsiWI
BsmI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI