Restriction map for
pF1KB7803
(Circular) (Six-base) MAPSORT of: pF1KB7803.seq Check: 7474 from: 1 to: 4622
pF1KB7803 4622 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1518 1518
Size: 4622
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1094 2447 2879 3626 3894 833
Size: 261 1353 432 747 268 1561
Fragments arranged by size:
1561 1353 747 432 268 261
AflIII A'CryG_T
Cuts at: 433 1974 3078 4055 433
Size: 1541 1104 977 1000
Fragments arranged by size:
1541 1104 1000 977
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AhdI GACnn_n'nnGTC
Cuts at: 609 1393 609
Size: 784 3838
AlwNI CAG_nnn'CTG
Cuts at: 298 334 694 1927 3494 298
Size: 36 360 1233 1567 1426
Fragments arranged by size:
1567 1426 1233 360 36
ApaLI G'TGCA_C
Cuts at: 3392 3392
Size: 4622
ApoI r'AATT_y
Cuts at: 683 1626 3975 4231 683
Size: 943 2349 256 1074
Fragments arranged by size:
2349 1074 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 250 1642 250
Size: 1392 3230
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 1638 2052 2300 2335 1638
Size: 414 248 35 3925
Fragments arranged by size:
3925 414 248 35
BanII G_rGCy'C
Cuts at: 1636 2666 1636
Size: 1030 3592
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbsI GAAGACnn'nnnn_
Cuts at: 239 608 941 239
Size: 369 333 3920
Fragments arranged by size:
3920 369 333
BbvCI CC'TCA_GC
Cuts at: 1364 1364
Size: 4622
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 1099 1246 1276 1360 1099
Size: 147 30 84 4361
Fragments arranged by size:
4361 147 84 30
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 2247 2340 3396 2247
Size: 93 1056 3473
Fragments arranged by size:
3473 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1834 1915 2250 4394 1834
Size: 81 335 2144 2062
Fragments arranged by size:
2144 2062 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 276 669 2989 276
Size: 393 2320 1909
Fragments arranged by size:
2320 1909 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1457 628
Size: 829 3793
Bpu10I CC'TnA_GC
Cuts at: 1364 1364
Size: 4622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1798 2677 3169 3467 730
Size: 1068 879 492 298 1885
Fragments arranged by size:
1885 1068 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4326 4326
Size: 4622
BsaAI yAC'GTr
Cuts at: 1975 1975
Size: 4622
BsaBI GATnn'nnATC
Cuts at: 6 1213 2136 4173 6
Size: 1207 923 2037 455
Fragments arranged by size:
2037 1207 923 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 416 836 1811 2332 3010 3284 3431 3842 416
Size: 420 975 521 678 274 147 411 1196
Fragments arranged by size:
1196 975 678 521 420 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1295 1325 1295
Size: 30 4592
BseYI C'CCAG_C
Cuts at: 170 716 776 2047 2805 3382 3878 170
Size: 546 60 1271 758 577 496 914
Fragments arranged by size:
1271 914 758 577 546 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 572 1546 199
Size: 373 974 3275
Fragments arranged by size:
3275 974 373
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3418 104
Size: 2060 46 1208 1308
Fragments arranged by size:
2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1431 1636 2414 2604 3396 1431
Size: 205 778 190 792 2657
Fragments arranged by size:
2657 792 778 205 190
Bsp1286I G_dGCh'C
Cuts at: 1431 1636 2247 2340 2414 2604 2666 3396 1431
Size: 205 611 93 74 190 62 730 2657
Fragments arranged by size:
2657 730 611 205 190 93 74 62
BspEI T'CCGG_A
Cuts at: 416 836 3842 416
Size: 420 3006 1196
Fragments arranged by size:
3006 1196 420
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 174 319 1518 1672 1886 2188 2569 174
Size: 145 1199 154 214 302 381 2227
Fragments arranged by size:
2227 1199 381 302 214 154 145
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2534 2534
Size: 4622
BsrFI r'CCGG_y
Cuts at: 2620 2801 3010 2620
Size: 181 209 4232
Fragments arranged by size:
4232 209 181
BssSI C'ACGA_G
Cuts at: 479 2893 3251 479
Size: 2414 358 1850
Fragments arranged by size:
2414 1850 358
BstAPI GCAn_nnn'nTGC
Cuts at: 216 1999 216
Size: 1783 2839
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstEII G'GTnAC_C
Cuts at: 1128 1494 1128
Size: 366 4256
BstYI r'GATC_y
Cuts at: 1 1466 1647 2114 2137 2472 2718 3730 3816
Size: 1465 181 467 23 335 246 1012 86
Cuts at: 3816 3828 4174 1
Size: 12 346 449
Fragments arranged by size:
1465 1012 467 449 346 335 246 181
86 23 12
BtgI C'CryG_G
Cuts at: 107 1124 1157 1535 1577 107
Size: 1017 33 378 42 3152
Fragments arranged by size:
3152 1017 378 42 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 170 1280 1865 170
Size: 1110 585 2927
Fragments arranged by size:
2927 1110 585
DraI TTT'AAA
Cuts at: 540 1621 540
Size: 1081 3541
DraIII CAC_nnn'GTG
Cuts at: 123 123
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 1570 2328 3186 1570
Size: 758 858 3006
Fragments arranged by size:
3006 858 758
EaeI y'GGCC_r
Cuts at: 344 1532 1584 2207 2381 2772 2799 4144 344
Size: 1188 52 623 174 391 27 1345 822
Fragments arranged by size:
1345 1188 822 623 391 174 52 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 415 851 2645 2855 415
Size: 436 1794 210 2182
Fragments arranged by size:
2182 1794 436 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3140 3286 4432 2830
Size: 310 146 1146 3020
Fragments arranged by size:
3020 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1634 1634
Size: 4622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 833 1094 1457 2447 2879 3626 3894 628
Size: 205 261 363 990 432 747 268 1356
Fragments arranged by size:
1356 990 747 432 363 268 261 205
EcoNI CCTnn'n_nnAGG
Cuts at: 1363 1507 1363
Size: 144 4478
EcoO109I rG'GnC_Cy
Cuts at: 331 1761 331
Size: 1430 3192
EcoRI G'AATT_C
Cuts at: 683 1626 683
Size: 943 3679
EcoRV GAT'ATC
Cuts at: 819 1559 819
Size: 740 3882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4622
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 1324 2056 2304 3326 4305 1324
Size: 732 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 732 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 300 332 595 627 1295 1327 1379
Size: 32 48 32 263 32 668 32 52
Cuts at: 1379 1411 2464 2494 2496 2526 220
Size: 32 1053 30 2 30 2316
Fragments arranged by size:
2316 1053 668 263 52 48 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 651 1661 651
Size: 1010 3612
HpaI GTT'AAC
Cuts at: 651 651
Size: 4622
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1404 3293 3477 1404
Size: 1889 184 2549
Fragments arranged by size:
2549 1889 184
MscI TGG'CCA
Cuts at: 1534 2383 1534
Size: 849 3773
MslI CAynn'nnrTG
Cuts at: 2611 2738 2611
Size: 127 4495
MspA1I CmG'CkG
Cuts at: 298 325 349 1733 2047 2407 3420 3665 298
Size: 27 24 1384 314 360 1013 245 1255
Fragments arranged by size:
1384 1255 1013 360 314 245 27 24
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 1535 1577 107
Size: 1428 42 3152
Fragments arranged by size:
3152 1428 42
NdeI CA'TA_TG
Cuts at: 140 140
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NspI r_CATG'y
Cuts at: 163 1482 1675 2175 3082 163
Size: 1319 193 500 907 1703
Fragments arranged by size:
1703 1319 907 500 193
PasI CC'CwG_GG
Cuts at: 824 1509 824
Size: 685 3937
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 806 1251 1491 1593 806
Size: 445 240 102 3835
Fragments arranged by size:
3835 445 240 102
PfoI T'CCnGG_A
Cuts at: 596 596
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 331 331
Size: 4622
PshAI GACnn'nnGTC
Cuts at: 1024 1024
Size: 4622
PsiI TTA'TAA
Cuts at: 917 917
Size: 4622
PstI C_TGCA'G
Cuts at: 330 1669 2354 330
Size: 1339 685 2598
Fragments arranged by size:
2598 1339 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 298 325 2047 2407 298
Size: 27 1722 360 2513
Fragments arranged by size:
2513 1722 360 27
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 1636 1636
Size: 4622
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 851 2645 2855 851
Size: 1794 210 2618
Fragments arranged by size:
2618 1794 210
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 751 4139 751
Size: 3388 1234
SfcI C'TryA_G
Cuts at: 33 326 755 1022 1307 1665 2350 3343 3534
Size: 293 429 267 285 358 685 993 191
Cuts at: 3534 33
Size: 1121
Fragments arranged by size:
1121 993 685 429 358 293 285 267
191
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 1644 1644
Size: 4622
SmlI C'TyrA_G
Cuts at: 745 1777 2692 3184 3446 745
Size: 1032 915 492 262 1921
Fragments arranged by size:
1921 1032 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StyI C'CwwG_G
Cuts at: 107 1535 1577 1756 2110 107
Size: 1428 42 179 354 2619
Fragments arranged by size:
2619 1428 354 179 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1258 2205 2338 2797 3006 1258
Size: 947 133 459 209 2874
Fragments arranged by size:
2874 947 459 209 133
TatI w'GTAC_w
Cuts at: 581 749 4137 581
Size: 168 3388 1066
Fragments arranged by size:
3388 1066 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4590
Tth111I GACn'n_nGTC
Cuts at: 2419 2419
Size: 4622
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1248 1450 2117 1248
Size: 202 667 3753
Fragments arranged by size:
3753 667 202
XmaI C'CCGG_G
Cuts at: 1642 1642
Size: 4622
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI FseI FspAI HindIII MfeI NheI NotI NruI
NsiI PacI PmlI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI