Restriction map for
pF1KB7795
(Circular) (Six-base) MAPSORT of: pF1KB7795.seq Check: 286 from: 1 to: 4496
pF1KB7795 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 367 1512 367
Size: 1145 3351
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 382 455 518 1498 2321 2753 3500 3768
Size: 143 73 63 980 823 432 747 268
Cuts at: 3768 239
Size: 967
Fragments arranged by size:
980 967 823 747 432 268 143 73
63
AfeI AGC'GCT
Cuts at: 952 952
Size: 4496
AflIII A'CryG_T
Cuts at: 1327 1848 2952 3929 1327
Size: 521 1104 977 1894
Fragments arranged by size:
1894 1104 977 521
AgeI A'CCGG_T
Cuts at: 654 2884 654
Size: 2230 2266
AlwNI CAG_nnn'CTG
Cuts at: 244 637 1801 3368 244
Size: 393 1164 1567 1372
Fragments arranged by size:
1567 1372 1164 393
ApaI G_GGCC'C
Cuts at: 1162 1162
Size: 4496
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 264 853 934 1245 1516 264
Size: 589 81 311 271 3244
Fragments arranged by size:
3244 589 311 271 81
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 367 848 1025 1202 1512 1926 2174 2209 367
Size: 481 177 177 310 414 248 35 2654
Fragments arranged by size:
2654 481 414 310 248 177 177 35
BanII G_rGCy'C
Cuts at: 177 377 667 682 924 1064 1162 1510 2540
Size: 200 290 15 242 140 98 348 1030
Cuts at: 2540 177
Size: 2133
Fragments arranged by size:
2133 1030 348 290 242 200 140 98
15
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 245 245
Size: 4496
BbvCI CC'TCA_GC
Cuts at: 564 751 564
Size: 187 4309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 888 922 1242 1276 888
Size: 34 320 34 4108
Fragments arranged by size:
4108 320 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 331 791 331
Size: 460 4036
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1189 1608 1189
Size: 419 4077
Bme1580I G_kGCm'C
Cuts at: 1018 1162 2121 2214 3270 1018
Size: 144 959 93 1056 2244
Fragments arranged by size:
2244 1056 959 144 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 972 2863 972
Size: 1891 2605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 595 160
Size: 435 4061
Bpu10I CC'TnA_GC
Cuts at: 564 751 564
Size: 187 4309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1328 1849 1328
Size: 521 3975
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 654 1685 2206 2884 3158 3305 3716 654
Size: 1031 521 678 274 147 411 1434
Fragments arranged by size:
1434 1031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 503 508 533 478
Size: 25 5 25 4441
Fragments arranged by size:
4441 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 336
Size: 4496
BseYI C'CCAG_C
Cuts at: 514 729 814 1921 2679 3256 3752 514
Size: 215 85 1107 758 577 496 1258
Fragments arranged by size:
1258 1107 758 577 496 215 85
BsiEI CG_ry'CG
Cuts at: 104 1350 2038 2084 3292 104
Size: 1246 688 46 1208 1308
Fragments arranged by size:
1308 1246 1208 688 46
BsiHKAI G_wGCw'C
Cuts at: 667 860 1268 1510 2288 2478 3270 667
Size: 193 408 242 778 190 792 1893
Fragments arranged by size:
1893 792 778 408 242 193 190
BsmI GAATG_Cn'
Cuts at: 1234 1234
Size: 4496
BsmBI CGTCTCn'nnnn_
Cuts at: 539 539
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 177 377 667 682 860 924 1018 1064 1162
Size: 200 290 15 178 64 94 46 98
Cuts at: 1162 1268 1510 2121 2214 2288 2478 2540 3270
Size: 106 242 611 93 74 190 62 730
Cuts at: 3270 177
Size: 1403
Fragments arranged by size:
1403 730 611 290 242 200 190 178
106 98 94 93 74 64 62 46
15
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1419 2788 2842 4326 4491 50
Size: 1369 1369 54 1484 165 55
Fragments arranged by size:
1484 1369 1369 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 654 1345 1447 2494 2675 2884 654
Size: 691 102 1047 181 209 2266
Fragments arranged by size:
2266 1047 691 209 181 102
BsrGI T'GTAC_A
Cuts at: 284 284
Size: 4496
BssHII G'CGCG_C
Cuts at: 1193 1443 1193
Size: 250 4246
BssSI C'ACGA_G
Cuts at: 1084 1267 2767 3125 1084
Size: 183 1500 358 2455
Fragments arranged by size:
2455 1500 358 183
BstAPI GCAn_nnn'nTGC
Cuts at: 296 1229 1406 1873 296
Size: 933 177 467 2919
Fragments arranged by size:
2919 933 467 177
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstEII G'GTnAC_C
Cuts at: 1002 1002
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 888 888
Size: 4496
BstYI r'GATC_y
Cuts at: 1 1521 1988 2011 2346 2592 3604 3690 3702
Size: 1520 467 23 335 246 1012 86 12
Cuts at: 3702 4048 1
Size: 346 449
Fragments arranged by size:
1520 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1739 1739
Size: 4496
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 1216 2202 3060 1216
Size: 986 858 2652
Fragments arranged by size:
2652 986 858
EaeI y'GGCC_r
Cuts at: 222 1347 2081 2255 2646 2673 4018 222
Size: 1125 734 174 391 27 1345 700
Fragments arranged by size:
1345 1125 734 700 391 174 27
EagI C'GGCC_G
Cuts at: 1347 2081 1347
Size: 734 3762
EarI CTCTTCn'nnn_
Cuts at: 354 1489 2519 2729 354
Size: 1135 1030 210 2121
Fragments arranged by size:
2121 1135 1030 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 1508 665
Size: 843 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 239 382 455 518 595 1498 2321 2753
Size: 79 143 73 63 77 903 823 432
Cuts at: 2753 3500 3768 160
Size: 747 268 888
Fragments arranged by size:
903 888 823 747 432 268 143 79
77 73 63
EcoNI CCTnn'n_nnAGG
Cuts at: 547 547
Size: 4496
EcoO109I rG'GnC_Cy
Cuts at: 114 1158 1159 1430 1635 114
Size: 1044 1 271 205 2975
Fragments arranged by size:
2975 1044 271 205 1
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 2515 1449
Size: 1066 3430
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 189 954 1189 1930 2178 3200 4179 189
Size: 765 235 741 248 1022 979 506
Fragments arranged by size:
1022 979 765 741 506 248 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 503 510 535 2338 2368 2370 2400 478
Size: 25 7 25 1803 30 2 30 2574
Fragments arranged by size:
2574 1803 30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 371 1516 371
Size: 1145 3351
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 770 1014 3167 3351 770
Size: 244 2153 184 1915
Fragments arranged by size:
2153 1915 244 184
MscI TGG'CCA
Cuts at: 224 2257 224
Size: 2033 2463
MslI CAynn'nnrTG
Cuts at: 2485 2612 2485
Size: 127 4369
MspA1I CmG'CkG
Cuts at: 356 1128 1352 1607 1921 2281 3294 3539 356
Size: 772 224 255 314 360 1013 245 1313
Fragments arranged by size:
1313 1013 772 360 314 255 245 224
NaeI GCC'GGC
Cuts at: 1449 2677 1449
Size: 1228 3268
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 1447 2675 1447
Size: 1228 3268
NspI r_CATG'y
Cuts at: 162 1400 1549 2049 2956 162
Size: 1238 149 500 907 1702
Fragments arranged by size:
1702 1238 907 500 149
PasI CC'CwG_GG
Cuts at: 888 1365 888
Size: 477 4019
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 220 637 967 220
Size: 417 330 3749
Fragments arranged by size:
3749 417 330
PfoI T'CCnGG_A
Cuts at: 1166 1436 1166
Size: 270 4226
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 1328 1328
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 114 1430 114
Size: 1316 3180
PspOMI G'GGCC_C
Cuts at: 1158 1158
Size: 4496
PstI C_TGCA'G
Cuts at: 324 743 1127 1225 1543 2228 324
Size: 419 384 98 318 685 2592
Fragments arranged by size:
2592 685 419 384 318 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1128 1921 2281 1128
Size: 793 360 3343
Fragments arranged by size:
3343 793 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 667 1510 667
Size: 843 3653
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 1489 2519 2729 1489
Size: 1030 210 3256
Fragments arranged by size:
3256 1030 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 320 739 1123 1221 1539 2224 3217 3408
Size: 287 419 384 98 318 685 993 191
Cuts at: 3408 33
Size: 1121
Fragments arranged by size:
1121 993 685 419 384 318 287 191
98
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SgrAI Cr'CCGG_yG
Cuts at: 654 654
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3058 3320 1651
Size: 915 492 262 2827
Fragments arranged by size:
2827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StuI AGG'CCT
Cuts at: 785 785
Size: 4496
StyI C'CwwG_G
Cuts at: 107 163 1010 1424 1630 1984 107
Size: 56 847 414 206 354 2619
Fragments arranged by size:
2619 847 414 354 206 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 296 787 1043 2079 2212 2671 2880 296
Size: 491 256 1036 133 459 209 1912
Fragments arranged by size:
1912 1036 491 459 256 209 133
TatI w'GTAC_w
Cuts at: 284 4011 284
Size: 3727 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 240 2293 240
Size: 2053 2443
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 360 576 1991 114
Size: 246 216 1415 2619
Fragments arranged by size:
2619 1415 246 216
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 1294 1483 1294
Size: 189 4307
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI