Restriction map for
pF1KB7787
(Circular) (Six-base) MAPSORT of: pF1KB7787.seq Check: 6719 from: 1 to: 4487
pF1KB7787 4487 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1409 1525 1409
Size: 116 4371
Acc65I G'GTAC_C
Cuts at: 269 1503 269
Size: 1234 3253
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 487 600 1188 2312 2744 3491 3759 452
Size: 35 113 588 1124 432 747 268 1180
Fragments arranged by size:
1180 1124 747 588 432 268 113 35
AflIII A'CryG_T
Cuts at: 502 1839 2943 3920 502
Size: 1337 1104 977 1069
Fragments arranged by size:
1337 1104 1069 977
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AhdI GACnn_n'nnGTC
Cuts at: 405 405
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 1792 3359 1792
Size: 1567 2920
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4487
ApaLI G'TGCA_C
Cuts at: 169 3257 169
Size: 3088 1399
ApoI r'AATT_y
Cuts at: 243 425 1154 1238 1459 1491 3840 4096 243
Size: 182 729 84 221 32 2349 256 634
Fragments arranged by size:
2349 729 634 256 221 182 84 32
AseI AT'TA_AT
Cuts at: 20 415 20
Size: 395 4092
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 1507 1507
Size: 4487
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 269 725 1145 1503 1917 2165 2200 269
Size: 456 420 358 414 248 35 2556
Fragments arranged by size:
2556 456 420 414 358 248 35
BanII G_rGCy'C
Cuts at: 219 1501 2531 219
Size: 1282 1030 2175
Fragments arranged by size:
2175 1282 1030
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbsI GAAGACnn'nnnn_
Cuts at: 1247 1247
Size: 4487
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 803 2007 803
Size: 1204 3283
BglI GCCn_nnn'nGGC
Cuts at: 214 214
Size: 4487
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 1599 1599
Size: 4487
Bme1580I G_kGCm'C
Cuts at: 173 219 2112 2205 3261 173
Size: 46 1893 93 1056 1399
Fragments arranged by size:
1893 1399 1056 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 268 668 920 1488 1699 1780 2115 4259 268
Size: 400 252 568 211 81 335 2144 496
Fragments arranged by size:
2144 568 496 400 335 252 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 494 2854 494
Size: 2360 2127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 1016 1352 1436 764
Size: 252 336 84 3815
Fragments arranged by size:
3815 336 252 84
Bpu10I CC'TnA_GC
Cuts at: 1211 1211
Size: 4487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1663 2542 3034 3332 1420
Size: 243 879 492 298 2575
Fragments arranged by size:
2575 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 382 744 4191 382
Size: 362 3447 678
Fragments arranged by size:
3447 678 362
BsaAI yAC'GTr
Cuts at: 505 1840 505
Size: 1335 3152
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 1918 2166 2868 4364 1918
Size: 248 702 1496 2041
Fragments arranged by size:
2041 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1676 2197 2875 3149 3296 3707 1676
Size: 521 678 274 147 411 2456
Fragments arranged by size:
2456 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 781 1180 613
Size: 168 399 3920
Fragments arranged by size:
3920 399 168
BseYI C'CCAG_C
Cuts at: 351 1385 1912 2670 3247 3743 351
Size: 1034 527 758 577 496 1095
Fragments arranged by size:
1095 1034 758 577 527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 4487
BsiEI CG_ry'CG
Cuts at: 104 2029 2075 3283 104
Size: 1925 46 1208 1308
Fragments arranged by size:
1925 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 1212 1501 2279 2469 3261 173
Size: 1039 289 778 190 792 1399
Fragments arranged by size:
1399 1039 792 778 289 190
Bsp1286I G_dGCh'C
Cuts at: 173 219 1212 1501 2112 2205 2279 2469 2531
Size: 46 993 289 611 93 74 190 62
Cuts at: 2531 3261 173
Size: 730 1399
Fragments arranged by size:
1399 993 730 611 289 190 93 74
62 46
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 294 1537 1751 2053 2434 294
Size: 1243 214 302 381 2347
Fragments arranged by size:
2347 1243 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2399 2399
Size: 4487
BsrFI r'CCGG_y
Cuts at: 2485 2666 2875 2485
Size: 181 209 4097
Fragments arranged by size:
4097 209 181
BsrGI T'GTAC_A
Cuts at: 847 847
Size: 4487
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstEII G'GTnAC_C
Cuts at: 147 1393 147
Size: 1246 3241
BstYI r'GATC_y
Cuts at: 1 1512 1979 2002 2337 2583 3595 3681 3693
Size: 1511 467 23 335 246 1012 86 12
Cuts at: 3693 4039 1
Size: 346 449
Fragments arranged by size:
1511 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 1730 1730
Size: 4487
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 2072 2246 2637 2664 4009 2072
Size: 174 391 27 1345 2550
Fragments arranged by size:
2550 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2072 2072
Size: 4487
EarI CTCTTCn'nnn_
Cuts at: 463 2510 2720 463
Size: 2047 210 2230
Fragments arranged by size:
2230 2047 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2695 3005 3151 4297 2695
Size: 310 146 1146 2885
Fragments arranged by size:
2885 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1499 1499
Size: 4487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 487 600 764 1016 1188 1352 1436 2312
Size: 35 113 164 252 172 164 84 876
Cuts at: 2312 2744 3491 3759 452
Size: 432 747 268 1180
Fragments arranged by size:
1180 876 747 432 268 252 172 164
164 113 84 35
EcoNI CCTnn'n_nnAGG
Cuts at: 576 576
Size: 4487
EcoO109I rG'GnC_Cy
Cuts at: 215 216 327 1626 215
Size: 1 111 1299 3076
Fragments arranged by size:
3076 1299 111 1
EcoRI G'AATT_C
Cuts at: 1154 1238 1491 1154
Size: 84 253 4150
Fragments arranged by size:
4150 253 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 602 2506 602
Size: 1904 2583
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 1921 2169 3191 4170 1921
Size: 248 1022 979 2238
Fragments arranged by size:
2238 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 741 773 795 827 1035 1067 2329
Size: 32 318 32 22 32 208 32 1262
Cuts at: 2329 2359 2361 2391 391
Size: 30 2 30 2487
Fragments arranged by size:
2487 1262 318 208 32 32 32 32
30 30 22 2
HincII GTy'rAC
Cuts at: 1526 1526
Size: 4487
HindIII A'AGCT_T
Cuts at: 947 1032 947
Size: 85 4402
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 273 1507 273
Size: 1234 3253
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 3158 3342 218
Size: 2940 184 1363
Fragments arranged by size:
2940 1363 184
MscI TGG'CCA
Cuts at: 2248 2248
Size: 4487
MslI CAynn'nnrTG
Cuts at: 2476 2603 2476
Size: 127 4360
MspA1I CmG'CkG
Cuts at: 465 1598 1912 2272 3285 3530 465
Size: 1133 314 360 1013 245 1422
Fragments arranged by size:
1422 1133 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 4487
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 107
Size: 4487
NdeI CA'TA_TG
Cuts at: 592 592
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 4487
NspI r_CATG'y
Cuts at: 1408 1540 2040 2947 1408
Size: 132 500 907 2948
Fragments arranged by size:
2948 907 500 132
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 144 144
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PshAI GACnn'nnGTC
Cuts at: 1439 1439
Size: 4487
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4487
PstI C_TGCA'G
Cuts at: 1534 2219 1534
Size: 685 3802
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 465 1912 2272 465
Size: 1447 360 2680
Fragments arranged by size:
2680 1447 360
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 1501 1501
Size: 4487
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 2510 2720 2510
Size: 210 4277
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SfcI C'TryA_G
Cuts at: 33 1530 2215 3208 3399 33
Size: 1497 685 993 191 1121
Fragments arranged by size:
1497 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 1399 1642 2557 3049 3311 1399
Size: 243 915 492 262 2575
Fragments arranged by size:
2575 915 492 262 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
StuI AGG'CCT
Cuts at: 1369 1369
Size: 4487
StyI C'CwwG_G
Cuts at: 107 549 1621 1975 107
Size: 442 1072 354 2619
Fragments arranged by size:
2619 1072 442 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2203 2662 2871 2070
Size: 133 459 209 3686
Fragments arranged by size:
3686 459 209 133
TatI w'GTAC_w
Cuts at: 847 4002 847
Size: 3155 1332
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4004 4036 4004
Size: 32 4455
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1982 1982
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
XmnI GAAnn'nnTTC
Cuts at: 586 613 865 1044 586
Size: 27 252 179 4029
Fragments arranged by size:
4029 252 179 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI