Restriction map for
pF1KB7784
(Circular) (Six-base) MAPSORT of: pF1KB7784.seq Check: 7543 from: 1 to: 4643
pF1KB7784 4643 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1681 1681
Size: 4643
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 1637 4483 1637
Size: 2846 1797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1212 2468 2900 3647 3915 301
Size: 911 1256 432 747 268 1029
Fragments arranged by size:
1256 1029 911 747 432 268
AfeI AGC'GCT
Cuts at: 249 249
Size: 4643
AflIII A'CryG_T
Cuts at: 1540 1995 3099 4076 1540
Size: 455 1104 977 2107
Fragments arranged by size:
2107 1104 977 455
AgeI A'CCGG_T
Cuts at: 3031 3031
Size: 4643
AleI CACnn'nnGTG
Cuts at: 1523 1523
Size: 4643
AlwNI CAG_nnn'CTG
Cuts at: 339 399 468 1948 3515 339
Size: 60 69 1480 1567 1467
Fragments arranged by size:
1567 1480 1467 69 60
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 481 1262 1430 1647 3996 4252 481
Size: 781 168 217 2349 256 872
Fragments arranged by size:
2349 872 781 256 217 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 1663 1663
Size: 4643
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4643
BanI G'GyrC_C
Cuts at: 1659 2073 2321 2356 1659
Size: 414 248 35 3946
Fragments arranged by size:
3946 414 248 35
BanII G_rGCy'C
Cuts at: 605 1657 2687 605
Size: 1052 1030 2561
Fragments arranged by size:
2561 1052 1030
BbeI G_GCGC'C
Cuts at: 2077 2325 2077
Size: 248 4395
BbsI GAAGACnn'nnnn_
Cuts at: 883 883
Size: 4643
BbvCI CC'TCA_GC
Cuts at: 340 340
Size: 4643
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3302 4643 2541
Size: 761 1341 2541
Fragments arranged by size:
2541 1341 761
BclI T'GATC_A
Cuts at: 720 960 2163 720
Size: 240 1203 3200
Fragments arranged by size:
3200 1203 240
BglII A'GATC_T
Cuts at: 368 1326 2158 4195 368
Size: 958 832 2037 816
Fragments arranged by size:
2037 958 832 816
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4643
Bme1580I G_kGCm'C
Cuts at: 2268 2361 3417 2268
Size: 93 1056 3494
Fragments arranged by size:
3494 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 438 1855 1936 2271 4415 438
Size: 1417 81 335 2144 666
Fragments arranged by size:
2144 1417 666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 3010 372
Size: 2638 2005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 285 1292 180
Size: 105 1007 3531
Fragments arranged by size:
3531 1007 105
Bpu10I CC'TnA_GC
Cuts at: 340 400 340
Size: 60 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 319 892 1819 2698 3190 3488 126
Size: 193 573 927 879 492 298 1281
Fragments arranged by size:
1281 927 879 573 492 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 412 4347 412
Size: 3935 708
BsaAI yAC'GTr
Cuts at: 1996 1996
Size: 4643
BsaBI GATnn'nnATC
Cuts at: 6 2157 4194 6
Size: 2151 2037 455
Fragments arranged by size:
2151 2037 455
BsaHI Gr'CG_yC
Cuts at: 2074 2322 3024 4520 2074
Size: 248 702 1496 2197
Fragments arranged by size:
2197 1496 702 248
BsaWI w'CCGG_w
Cuts at: 289 1832 2353 3031 3305 3452 3863 289
Size: 1543 521 678 274 147 411 1069
Fragments arranged by size:
1543 1069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 258 288 258
Size: 30 4613
BseYI C'CCAG_C
Cuts at: 534 666 692 2068 2826 3403 3899 534
Size: 132 26 1376 758 577 496 1278
Fragments arranged by size:
1376 1278 758 577 496 132 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 454
Size: 4643
BsiEI CG_ry'CG
Cuts at: 104 1576 2185 2231 3439 104
Size: 1472 609 46 1208 1308
Fragments arranged by size:
1472 1308 1208 609 46
BsiHKAI G_wGCw'C
Cuts at: 554 1657 2435 2625 3417 554
Size: 1103 778 190 792 1780
Fragments arranged by size:
1780 1103 792 778 190
BsmI GAATG_Cn'
Cuts at: 404 404
Size: 4643
Bsp1286I G_dGCh'C
Cuts at: 554 605 1657 2268 2361 2435 2625 2687 3417
Size: 51 1052 611 93 74 190 62 730
Cuts at: 3417 554
Size: 1780
Fragments arranged by size:
1780 1052 730 611 190 93 74 62
51
BspEI T'CCGG_A
Cuts at: 3863 3863
Size: 4643
BspHI T'CATG_A
Cuts at: 279 1343 3819 279
Size: 1064 2476 1103
Fragments arranged by size:
2476 1103 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1137 1693 1907 2209 2590 1137
Size: 556 214 302 381 3190
Fragments arranged by size:
3190 556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2935 2989 4473 4638 50
Size: 2885 54 1484 165 55
Fragments arranged by size:
2885 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 2555
Size: 4643
BsrFI r'CCGG_y
Cuts at: 2641 2822 3031 2641
Size: 181 209 4253
Fragments arranged by size:
4253 209 181
BsrGI T'GTAC_A
Cuts at: 1083 1083
Size: 4643
BssSI C'ACGA_G
Cuts at: 2914 3272 2914
Size: 358 4285
BstAPI GCAn_nnn'nTGC
Cuts at: 2020 2020
Size: 4643
BstBI TT'CG_AA
Cuts at: 3004 3004
Size: 4643
BstYI r'GATC_y
Cuts at: 1 350 368 1164 1326 1668 2135 2158 2493
Size: 349 18 796 162 342 467 23 335
Cuts at: 2493 2739 3751 3837 3849 4195 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 796 467 449 349 346 342 335
246 162 86 23 18 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 2593 2774 2937 127
Size: 2466 181 163 1833
Fragments arranged by size:
2466 1833 181 163
BtsI GCAGTG_nn'
Cuts at: 554 575 1886 554
Size: 21 1311 3311
Fragments arranged by size:
3311 1311 21
DraI TTT'AAA
Cuts at: 1455 1642 1455
Size: 187 4456
DraIII CAC_nnn'GTG
Cuts at: 1081 1081
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 177 2349 3207 177
Size: 2172 858 1613
Fragments arranged by size:
2172 1613 858
EaeI y'GGCC_r
Cuts at: 2228 2402 2793 2820 4165 2228
Size: 174 391 27 1345 2706
Fragments arranged by size:
2706 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2228 2228
Size: 4643
EarI CTCTTCn'nnn_
Cuts at: 842 2666 2876 842
Size: 1824 210 2609
Fragments arranged by size:
2609 1824 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2851 3161 3307 4453 2851
Size: 310 146 1146 3041
Fragments arranged by size:
3041 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1655 1655
Size: 4643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 285 301 1212 1292 2468 2900 3647 3915
Size: 105 16 911 80 1176 432 747 268
Cuts at: 3915 180
Size: 908
Fragments arranged by size:
1176 911 908 747 432 268 105 80
16
EcoO109I rG'GnC_Cy
Cuts at: 1180 1782 1180
Size: 602 4041
EcoRI G'AATT_C
Cuts at: 481 1262 1430 1647 481
Size: 781 168 217 3477
Fragments arranged by size:
3477 781 217 168
EcoRV GAT'ATC
Cuts at: 906 906
Size: 4643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1578 2662 1578
Size: 1084 3559
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 251 2077 2325 3347 4326 251
Size: 1826 248 1022 979 568
Fragments arranged by size:
1826 1022 979 568 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1311 1343 2485 2515 2517 2547 124
Size: 32 1155 32 1142 30 2 30 2220
Fragments arranged by size:
2220 1155 1142 32 32 30 30 2
HincII GTy'rAC
Cuts at: 812 1294 1682 812
Size: 482 388 3773
Fragments arranged by size:
3773 482 388
HindIII A'AGCT_T
Cuts at: 1224 1392 1224
Size: 168 4475
KasI G'GCGC_C
Cuts at: 2073 2321 2073
Size: 248 4395
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3314 3498 3314
Size: 184 4459
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 112 1002 1371 1523 2632 2759 112
Size: 890 369 152 1109 127 1996
Fragments arranged by size:
1996 1109 890 369 152 127
MspA1I CmG'CkG
Cuts at: 666 1754 2068 2428 3441 3686 666
Size: 1088 314 360 1013 245 1623
Fragments arranged by size:
1623 1088 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2824 2824
Size: 4643
NarI GG'CG_CC
Cuts at: 2074 2322 2074
Size: 248 4395
NcoI C'CATG_G
Cuts at: 107 107
Size: 4643
NgoMIV G'CCGG_C
Cuts at: 2822 2822
Size: 4643
NspI r_CATG'y
Cuts at: 1544 1622 1696 2196 3103 1544
Size: 78 74 500 907 3084
Fragments arranged by size:
3084 907 500 78 74
PciI A'CATG_T
Cuts at: 1540 3099 1540
Size: 1559 3084
PflMI CCAn_nnn'nTGG
Cuts at: 534 612 534
Size: 78 4565
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4611
PstI C_TGCA'G
Cuts at: 1690 2375 1690
Size: 685 3958
PvuI CG_AT'CG
Cuts at: 104 1576 104
Size: 1472 3171
PvuII CAG'CTG
Cuts at: 666 2068 2428 666
Size: 1402 360 2881
Fragments arranged by size:
2881 1402 360
RsrII CG'GwC_CG
Cuts at: 292 2838 292
Size: 2546 2097
SacI G_AGCT'C
Cuts at: 1657 1657
Size: 4643
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 2666 2876 2666
Size: 210 4433
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 4160 4160
Size: 4643
SfcI C'TryA_G
Cuts at: 33 1686 2371 3364 3555 33
Size: 1653 685 993 191 1121
Fragments arranged by size:
1653 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2075 2323 2075
Size: 248 4395
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SmaI CCC'GGG
Cuts at: 1665 1665
Size: 4643
SmlI C'TyrA_G
Cuts at: 141 298 871 1798 2713 3205 3467 141
Size: 157 573 927 915 492 262 1317
Fragments arranged by size:
1317 927 915 573 492 262 157
SpeI A'CTAG_T
Cuts at: 67 172 67
Size: 105 4538
SphI G_CATG'C
Cuts at: 1696 2196 1696
Size: 500 4143
SspI AAT'ATT
Cuts at: 634 634
Size: 4643
StyI C'CwwG_G
Cuts at: 107 413 1108 1777 2131 107
Size: 306 695 669 354 2619
Fragments arranged by size:
2619 695 669 354 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 604 2226 2359 2818 3027 420
Size: 184 1622 133 459 209 2036
Fragments arranged by size:
2036 1622 459 209 184 133
TatI w'GTAC_w
Cuts at: 1083 4158 1083
Size: 3075 1568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 554 586 4160 4192 554
Size: 32 3574 32 1005
Fragments arranged by size:
3574 1005 32 32
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4643
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 584 2138 584
Size: 1554 3089
XmaI C'CCGG_G
Cuts at: 1663 1663
Size: 4643
XmnI GAAnn'nnTTC
Cuts at: 1225 1225
Size: 4643
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI