Restriction map for
pF1KB7779
(Circular) (Six-base) MAPSORT of: pF1KB7779.seq Check: 3351 from: 1 to: 4415
pF1KB7779 4415 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 1409 4255 1409
Size: 2846 1569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 518 816 2240 2672 3419 3687 333
Size: 185 298 1424 432 747 268 1061
Fragments arranged by size:
1424 1061 747 432 298 268 185
AfeI AGC'GCT
Cuts at: 344 344
Size: 4415
AflIII A'CryG_T
Cuts at: 645 679 1154 1767 2871 3848 645
Size: 34 475 613 1104 977 1212
Fragments arranged by size:
1212 1104 977 613 475 34
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 138 475 505 523 1072 1176 1720 3287 138
Size: 337 30 18 549 104 544 1567 1266
Fragments arranged by size:
1567 1266 549 544 337 104 30 18
ApaLI G'TGCA_C
Cuts at: 1390 3185 1390
Size: 1795 2620
ApoI r'AATT_y
Cuts at: 634 733 1110 1143 1290 1419 3768 4024 634
Size: 99 377 33 147 129 2349 256 1025
Fragments arranged by size:
2349 1025 377 256 147 129 99 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1435 1435
Size: 4415
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 1431 1845 2093 2128 1431
Size: 414 248 35 3718
Fragments arranged by size:
3718 414 248 35
BanII G_rGCy'C
Cuts at: 402 1429 2459 402
Size: 1027 1030 2358
Fragments arranged by size:
2358 1030 1027
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbvCI CC'TCA_GC
Cuts at: 880 880
Size: 4415
BciVI GTATCCnnnnn_n'
Cuts at: 963 1227 1392 2313 3074 4415 963
Size: 264 165 921 761 1341 963
Fragments arranged by size:
1341 963 921 761 264 165
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglII A'GATC_T
Cuts at: 1930 3967 1930
Size: 2037 2378
BlpI GC'TnA_GC
Cuts at: 1527 1527
Size: 4415
Bme1580I G_kGCm'C
Cuts at: 1394 2040 2133 3189 1394
Size: 646 93 1056 2620
Fragments arranged by size:
2620 1056 646 93
BmgBI CAC'GTC
Cuts at: 682 682
Size: 4415
BmrI ACTGGGnnnn_n'
Cuts at: 890 1627 1708 2043 4187 890
Size: 737 81 335 2144 1118
Fragments arranged by size:
2144 1118 737 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 628 545
Size: 83 4332
Bpu10I CC'TnA_GC
Cuts at: 880 880
Size: 4415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 2962 3260 1591
Size: 879 492 298 2746
Fragments arranged by size:
2746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 388 4119 388
Size: 3731 684
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 1846 2094 2796 4292 1846
Size: 248 702 1496 1969
Fragments arranged by size:
1969 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1114 1604 2125 2803 3077 3224 3635 1114
Size: 490 521 678 274 147 411 1894
Fragments arranged by size:
1894 678 521 490 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1202 1232 1202
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 421 928 410
Size: 11 507 3897
Fragments arranged by size:
3897 507 11
BseYI C'CCAG_C
Cuts at: 425 1840 2598 3175 3671 425
Size: 1415 758 577 496 1169
Fragments arranged by size:
1415 1169 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1037 1096 190
Size: 847 59 3509
Fragments arranged by size:
3509 847 59
BsiEI CG_ry'CG
Cuts at: 104 1043 1957 2003 3211 104
Size: 939 914 46 1208 1308
Fragments arranged by size:
1308 1208 939 914 46
BsiHKAI G_wGCw'C
Cuts at: 1394 1429 2207 2397 3189 1394
Size: 35 778 190 792 2620
Fragments arranged by size:
2620 792 778 190 35
Bsp1286I G_dGCh'C
Cuts at: 402 1394 1429 2040 2133 2207 2397 2459 3189
Size: 992 35 611 93 74 190 62 730
Cuts at: 3189 402
Size: 1628
Fragments arranged by size:
1628 992 730 611 190 93 74 62
35
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 266 1160 3591 266
Size: 894 2431 1090
Fragments arranged by size:
2431 1090 894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1465 1679 1981 2362 1465
Size: 214 302 381 3518
Fragments arranged by size:
3518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 2413 2594 2803 2413
Size: 181 209 4025
Fragments arranged by size:
4025 209 181
BsrGI T'GTAC_A
Cuts at: 1298 1298
Size: 4415
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 505 1792 505
Size: 1287 3128
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstYI r'GATC_y
Cuts at: 1 1440 1907 1930 2265 2511 3523 3609 3621
Size: 1439 467 23 335 246 1012 86 12
Cuts at: 3621 3967 1
Size: 346 449
Fragments arranged by size:
1439 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 512 107
Size: 405 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 1658
Size: 4415
DraI TTT'AAA
Cuts at: 1316 1414 1316
Size: 98 4317
DraIII CAC_nnn'GTG
Cuts at: 168 168
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 140 159 204 920 1040 2000 2174 2565 2592
Size: 19 45 716 120 960 174 391 27
Cuts at: 2592 3937 140
Size: 1345 618
Fragments arranged by size:
1345 960 716 618 391 174 120 45
27 19
EagI C'GGCC_G
Cuts at: 1040 2000 1040
Size: 960 3455
EarI CTCTTCn'nnn_
Cuts at: 352 2438 2648 352
Size: 2086 210 2119
Fragments arranged by size:
2119 2086 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 2623 2933 3079 4225 232
Size: 2391 310 146 1146 422
Fragments arranged by size:
2391 1146 422 310 146
EcoICRI GAG'CTC
Cuts at: 1427 1427
Size: 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 518 545 628 816 2240 2672 3419 3687
Size: 185 27 83 188 1424 432 747 268
Cuts at: 3687 333
Size: 1061
Fragments arranged by size:
1424 1061 747 432 268 188 185 83
27
EcoNI CCTnn'n_nnAGG
Cuts at: 479 1373 479
Size: 894 3521
EcoO109I rG'GnC_Cy
Cuts at: 411 472 1199 1554 411
Size: 61 727 355 3272
Fragments arranged by size:
3272 727 355 61
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 346 1849 2097 3119 4098 346
Size: 1503 248 1022 979 663
Fragments arranged by size:
1503 1022 979 663 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2257 2287 2289 2319 103
Size: 32 2122 30 2 30 2199
Fragments arranged by size:
2199 2122 32 30 30 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MfeI C'AATT_G
Cuts at: 957 957
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1188 3086 3270 1188
Size: 1898 184 2333
Fragments arranged by size:
2333 1898 184
MscI TGG'CCA
Cuts at: 161 922 2176 161
Size: 761 1254 2400
Fragments arranged by size:
2400 1254 761
MslI CAynn'nnrTG
Cuts at: 748 940 1159 1165 2404 2531 748
Size: 192 219 6 1239 127 2632
Fragments arranged by size:
2632 1239 219 192 127 6
MspA1I CmG'CkG
Cuts at: 505 514 1051 1072 1526 1840 2200 3213 3458
Size: 9 537 21 454 314 360 1013 245
Cuts at: 3458 505
Size: 1462
Fragments arranged by size:
1462 1013 537 454 360 314 245 21
9
NaeI GCC'GGC
Cuts at: 2596 2596
Size: 4415
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NcoI C'CATG_G
Cuts at: 107 107
Size: 4415
NgoMIV G'CCGG_C
Cuts at: 2594 2594
Size: 4415
NspI r_CATG'y
Cuts at: 316 629 649 1158 1170 1468 1968 2875 316
Size: 313 20 509 12 298 500 907 1856
Fragments arranged by size:
1856 907 509 500 313 298 20 12
PciI A'CATG_T
Cuts at: 645 1154 2871 645
Size: 509 1717 2189
Fragments arranged by size:
2189 1717 509
PflMI CCAn_nnn'nTGG
Cuts at: 288 1147 288
Size: 859 3556
PfoI T'CCnGG_A
Cuts at: 121 121
Size: 4415
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 411 472 1199 411
Size: 61 727 3627
Fragments arranged by size:
3627 727 61
PstI C_TGCA'G
Cuts at: 134 510 1462 2147 134
Size: 376 952 685 2402
Fragments arranged by size:
2402 952 685 376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 505 1051 1072 1840 2200 505
Size: 546 21 768 360 2720
Fragments arranged by size:
2720 768 546 360 21
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1429 1429
Size: 4415
SacII CC_GC'GG
Cuts at: 515 515
Size: 4415
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SanDI GG'GwC_CC
Cuts at: 1199 1199
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 352 2438 2648 352
Size: 2086 210 2119
Fragments arranged by size:
2119 2086 210
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 130 506 1269 1370 1458 2143 3136 3327
Size: 97 376 763 101 88 685 993 191
Cuts at: 3327 33
Size: 1121
Fragments arranged by size:
1121 993 763 685 376 191 101 97
88
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 1570 2485 2977 3239 1570
Size: 915 492 262 2746
Fragments arranged by size:
2746 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
SspI AAT'ATT
Cuts at: 865 865
Size: 4415
StyI C'CwwG_G
Cuts at: 107 1194 1281 1549 1903 107
Size: 1087 87 268 354 2619
Fragments arranged by size:
2619 1087 354 268 87
SwaI ATTT'AAAT
Cuts at: 1316 1316
Size: 4415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1998 2131 2590 2799 1998
Size: 133 459 209 3614
Fragments arranged by size:
3614 459 209 133
TatI w'GTAC_w
Cuts at: 1298 3930 1298
Size: 2632 1783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1202 1234 3932 3964 1202
Size: 32 2698 32 1653
Fragments arranged by size:
2698 1653 32 32
Tth111I GACn'n_nGTC
Cuts at: 2212 2212
Size: 4415
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 915 1910 915
Size: 995 3420
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
StuI XhoI XmnI ZraI