Restriction map for
pF1KB7767
(Circular) (Six-base) MAPSORT of: pF1KB7767.seq Check: 3198 from: 1 to: 4454
pF1KB7767 4454 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1155 1155
Size: 4454
AatII G_ACGT'C
Cuts at: 920 920
Size: 4454
AccI GT'mk_AC
Cuts at: 1492 1492
Size: 4454
Acc65I G'GTAC_C
Cuts at: 184 1470 184
Size: 1286 3168
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2711 3458 3726 2279
Size: 432 747 268 3007
Fragments arranged by size:
3007 747 432 268
AflIII A'CryG_T
Cuts at: 1806 2910 3887 1806
Size: 1104 977 2373
Fragments arranged by size:
2373 1104 977
AgeI A'CCGG_T
Cuts at: 2842 2842
Size: 4454
AlwNI CAG_nnn'CTG
Cuts at: 152 1365 1759 3326 152
Size: 1213 394 1567 1280
Fragments arranged by size:
1567 1280 1213 394
ApaI G_GGCC'C
Cuts at: 223 617 1036 1197 1419 223
Size: 394 419 161 222 3258
Fragments arranged by size:
3258 419 394 222 161
ApaLI G'TGCA_C
Cuts at: 862 3224 862
Size: 2362 2092
ApoI r'AATT_y
Cuts at: 937 1260 1458 3807 4063 937
Size: 323 198 2349 256 1328
Fragments arranged by size:
2349 1328 323 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 119 767 904 1474 119
Size: 648 137 570 3099
Fragments arranged by size:
3099 648 570 137
BamHI G'GATC_C
Cuts at: 1479 1479
Size: 4454
BanI G'GyrC_C
Cuts at: 184 778 851 1470 1884 2132 2167 184
Size: 594 73 619 414 248 35 2471
Fragments arranged by size:
2471 619 594 414 248 73 35
BanII G_rGCy'C
Cuts at: 223 247 617 710 1036 1197 1419 1468 2498
Size: 24 370 93 326 161 222 49 1030
Cuts at: 2498 223
Size: 2179
Fragments arranged by size:
2179 1030 370 326 222 161 93 49
24
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4206
BbsI GAAGACnn'nnnn_
Cuts at: 391 1380 391
Size: 989 3465
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglI GCCn_nnn'nGGC
Cuts at: 763 763
Size: 4454
BglII A'GATC_T
Cuts at: 926 1969 4006 926
Size: 1043 2037 1374
Fragments arranged by size:
2037 1374 1043
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4454
Bme1580I G_kGCm'C
Cuts at: 223 617 781 866 1036 1197 1419 2079 2172
Size: 394 164 85 170 161 222 660 93
Cuts at: 2172 3228 223
Size: 1056 1449
Fragments arranged by size:
1449 1056 660 394 222 170 164 161
93 85
BmrI ACTGGGnnnn_n'
Cuts at: 565 1038 1666 1747 2082 4226 565
Size: 473 628 81 335 2144 793
Fragments arranged by size:
2144 793 628 473 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1222 1318 623
Size: 599 96 3759
Fragments arranged by size:
3759 599 96
Bpu10I CC'TnA_GC
Cuts at: 1289 1289
Size: 4454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3001 3299 1630
Size: 879 492 298 2785
Fragments arranged by size:
2785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1401 4158 1401
Size: 2757 1697
BsaAI yAC'GTr
Cuts at: 1807 1807
Size: 4454
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 917 1885 2133 2835 4331 917
Size: 968 248 702 1496 1040
Fragments arranged by size:
1496 1040 968 702 248
BsaWI w'CCGG_w
Cuts at: 1643 2164 2842 3116 3263 3674 1643
Size: 521 678 274 147 411 2423
Fragments arranged by size:
2423 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 1060 193
Size: 867 3587
BseYI C'CCAG_C
Cuts at: 124 205 521 753 883 1196 1418 1879 2637
Size: 81 316 232 130 313 222 461 758
Cuts at: 2637 3214 3710 124
Size: 577 496 868
Fragments arranged by size:
868 758 577 496 461 316 313 232
222 130 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1359 1359
Size: 4454
BsiEI CG_ry'CG
Cuts at: 104 300 1996 2042 3250 104
Size: 196 1696 46 1208 1308
Fragments arranged by size:
1696 1308 1208 196 46
BsiHKAI G_wGCw'C
Cuts at: 866 1468 2246 2436 3228 866
Size: 602 778 190 792 2092
Fragments arranged by size:
2092 792 778 602 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1177 1177
Size: 4454
Bsp1286I G_dGCh'C
Cuts at: 223 247 617 710 781 866 1036 1197 1419
Size: 24 370 93 71 85 170 161 222
Cuts at: 1419 1468 2079 2172 2246 2436 2498 3228 223
Size: 49 611 93 74 190 62 730 1449
Fragments arranged by size:
1449 730 611 370 222 190 170 161
93 93 85 74 71 62 49 24
BspEI T'CCGG_A
Cuts at: 3674 3674
Size: 4454
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1155 1504 1718 2020 2401 1155
Size: 349 214 302 381 3208
Fragments arranged by size:
3208 381 349 302 214
BsrBI CCG'CTC
Cuts at: 50 903 2746 2800 4284 4449 50
Size: 853 1843 54 1484 165 55
Fragments arranged by size:
1843 1484 853 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2366 2366
Size: 4454
BsrFI r'CCGG_y
Cuts at: 187 1057 2452 2633 2842 187
Size: 870 1395 181 209 1799
Fragments arranged by size:
1799 1395 870 209 181
BssSI C'ACGA_G
Cuts at: 2725 3083 2725
Size: 358 4096
BstAPI GCAn_nnn'nTGC
Cuts at: 1275 1831 1275
Size: 556 3898
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstYI r'GATC_y
Cuts at: 1 607 926 1479 1946 1969 2304 2550 3562
Size: 606 319 553 467 23 335 246 1012
Cuts at: 3562 3648 3660 4006 1
Size: 86 12 346 449
Fragments arranged by size:
1012 606 553 467 449 346 335 319
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 572 572
Size: 4454
BtgI C'CryG_G
Cuts at: 107 107
Size: 4454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 731 2404 2585 2748 731
Size: 1673 181 163 2437
Fragments arranged by size:
2437 1673 181 163
BtsI GCAGTG_nn'
Cuts at: 168 180 201 876 1080 1697 168
Size: 12 21 675 204 617 2925
Fragments arranged by size:
2925 675 617 204 21 12
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 278 297 2039 2213 2604 2631 3976 278
Size: 19 1742 174 391 27 1345 756
Fragments arranged by size:
1742 1345 756 391 174 27 19
EagI C'GGCC_G
Cuts at: 297 2039 297
Size: 1742 2712
EarI CTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1033 2662 2972 3118 4264 1033
Size: 1629 310 146 1146 1223
Fragments arranged by size:
1629 1223 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1222 1318 2279 2711 3458 3726 623
Size: 599 96 961 432 747 268 1351
Fragments arranged by size:
1351 961 747 599 432 268 96
EcoO109I rG'GnC_Cy
Cuts at: 219 273 559 614 771 1032 1415 1593 219
Size: 54 286 55 157 261 383 178 3080
Fragments arranged by size:
3080 383 286 261 178 157 55 54
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 898 1888 2136 3158 4137 898
Size: 990 248 1022 979 1215
Fragments arranged by size:
1215 1022 990 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 356 378 410 2296 2326 2328 2358 324
Size: 32 22 32 1886 30 2 30 2420
Fragments arranged by size:
2420 1886 32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 1493 1493
Size: 4454
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4206
KpnI G_GTAC'C
Cuts at: 188 1474 188
Size: 1286 3168
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 322 3125 3309 322
Size: 2803 184 1467
Fragments arranged by size:
2803 1467 184
MscI TGG'CCA
Cuts at: 280 2215 280
Size: 1935 2519
MslI CAynn'nnrTG
Cuts at: 2443 2570 2443
Size: 127 4327
MspA1I CmG'CkG
Cuts at: 149 795 1200 1284 1565 1879 2239 3252 3497
Size: 646 405 84 281 314 360 1013 245
Cuts at: 3497 149
Size: 1106
Fragments arranged by size:
1106 1013 646 405 360 314 281 245
84
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4454
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4206
NcoI C'CATG_G
Cuts at: 107 107
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4454
NotI GC'GGCC_GC
Cuts at: 297 297
Size: 4454
NspI r_CATG'y
Cuts at: 1507 2007 2914 1507
Size: 500 907 3047
Fragments arranged by size:
3047 907 500
PasI CC'CwG_GG
Cuts at: 697 697
Size: 4454
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PflMI CCAn_nnn'nTGG
Cuts at: 426 501 426
Size: 75 4379
PfoI T'CCnGG_A
Cuts at: 266 1313 266
Size: 1047 3407
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1355 1387 3593 3625 1355
Size: 32 2206 32 2184
Fragments arranged by size:
2206 2184 32 32
PpuMI rG'GwC_Cy
Cuts at: 559 559
Size: 4454
PspOMI G'GGCC_C
Cuts at: 219 613 1032 1193 1415 219
Size: 394 419 161 222 3258
Fragments arranged by size:
3258 419 394 222 161
PstI C_TGCA'G
Cuts at: 548 1501 2186 548
Size: 953 685 2816
Fragments arranged by size:
2816 953 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 795 1200 1284 1879 2239 795
Size: 405 84 595 360 3010
Fragments arranged by size:
3010 595 405 360 84
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4454
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SanDI GG'GwC_CC
Cuts at: 559 559
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SexAI A'CCwGG_T
Cuts at: 470 1253 470
Size: 783 3671
SfcI C'TryA_G
Cuts at: 33 544 1497 2182 3175 3366 33
Size: 511 953 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 511 191
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 121 1476 121
Size: 1355 3099
SmlI C'TyrA_G
Cuts at: 904 1609 2524 3016 3278 904
Size: 705 915 492 262 2080
Fragments arranged by size:
2080 915 705 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3954
StuI AGG'CCT
Cuts at: 577 577
Size: 4454
StyI C'CwwG_G
Cuts at: 107 479 1142 1588 1942 107
Size: 372 663 446 354 2619
Fragments arranged by size:
2619 663 446 372 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1372 2037 2170 2629 2838 1372
Size: 665 133 459 209 2988
Fragments arranged by size:
2988 665 459 209 133
TatI w'GTAC_w
Cuts at: 3969 3969
Size: 4454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 3971 4003 147
Size: 32 3792 32 598
Fragments arranged by size:
3792 598 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 598 1335 1949 598
Size: 737 614 3103
Fragments arranged by size:
3103 737 614
XhoI C'TCGA_G
Cuts at: 904 904
Size: 4454
XmaI C'CCGG_G
Cuts at: 119 1474 119
Size: 1355 3099
XmnI GAAnn'nnTTC
Cuts at: 643 643
Size: 4454
ZraI GAC'GTC
Cuts at: 918 918
Size: 4454
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI