Restriction map for
pF1KB7766
(Circular) (Six-base) MAPSORT of: pF1KB7766.seq Check: 9828 from: 1 to: 4622
pF1KB7766 4622 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 426 1660 426
Size: 1234 3388
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 2879 3626 3894 2447
Size: 432 747 268 3175
Fragments arranged by size:
3175 747 432 268
AflIII A'CryG_T
Cuts at: 1974 3078 4055 1974
Size: 1104 977 2541
Fragments arranged by size:
2541 1104 977
AgeI A'CCGG_T
Cuts at: 1493 3010 1493
Size: 1517 3105
AlwNI CAG_nnn'CTG
Cuts at: 1927 3494 1927
Size: 1567 3055
ApaLI G'TGCA_C
Cuts at: 3392 3392
Size: 4622
ApoI r'AATT_y
Cuts at: 142 186 297 435 574 582 686 851 895
Size: 44 111 138 139 8 104 165 44
Cuts at: 895 935 1019 1103 1288 1355 1439 1626 3975
Size: 40 84 84 185 67 84 187 2349
Cuts at: 3975 4231 142
Size: 256 533
Fragments arranged by size:
2349 533 256 187 185 165 139 138
111 104 84 84 84 67 44 44
40 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 1642 1642
Size: 4622
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 1638 2052 2300 2335 1638
Size: 414 248 35 3925
Fragments arranged by size:
3925 414 248 35
BanII G_rGCy'C
Cuts at: 1636 2666 1636
Size: 1030 3592
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbsI GAAGACnn'nnnn_
Cuts at: 222 310 222
Size: 88 4534
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 263 2142 263
Size: 1879 2743
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 2247 2340 3396 2247
Size: 93 1056 3473
Fragments arranged by size:
3473 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 325 1834 1915 2250 4394 325
Size: 1509 81 335 2144 553
Fragments arranged by size:
2144 1509 553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 716 881 965 1217 1385 1469 172
Size: 123 421 165 84 252 168 84 3325
Fragments arranged by size:
3325 421 252 168 165 123 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1798 2677 3169 3467 1798
Size: 879 492 298 2953
Fragments arranged by size:
2953 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4326 4326
Size: 4622
BsaAI yAC'GTr
Cuts at: 1975 1975
Size: 4622
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1493 1811 2332 3010 3284 3431 3842 1493
Size: 318 521 678 274 147 411 2273
Fragments arranged by size:
2273 678 521 411 318 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4622
BseYI C'CCAG_C
Cuts at: 2047 2805 3382 3878 2047
Size: 758 577 496 2791
Fragments arranged by size:
2791 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3418 104
Size: 2060 46 1208 1308
Fragments arranged by size:
2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1636 2414 2604 3396 1636
Size: 778 190 792 2862
Fragments arranged by size:
2862 792 778 190
BsmI GAATG_Cn'
Cuts at: 656 656
Size: 4622
Bsp1286I G_dGCh'C
Cuts at: 1636 2247 2340 2414 2604 2666 3396 1636
Size: 611 93 74 190 62 730 2862
Fragments arranged by size:
2862 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 4622
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1672 1886 2188 2569 1672
Size: 214 302 381 3725
Fragments arranged by size:
3725 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 169 2534 169
Size: 2365 2257
BsrFI r'CCGG_y
Cuts at: 1493 2620 2801 3010 1493
Size: 1127 181 209 3105
Fragments arranged by size:
3105 1127 209 181
BssSI C'ACGA_G
Cuts at: 2893 3251 2893
Size: 358 4264
BstAPI GCAn_nnn'nTGC
Cuts at: 1999 1999
Size: 4622
BstBI TT'CG_AA
Cuts at: 613 2983 613
Size: 2370 2252
BstYI r'GATC_y
Cuts at: 1 1647 2114 2137 2472 2718 3730 3816 3828
Size: 1646 467 23 335 246 1012 86 12
Cuts at: 3828 4174 1
Size: 346 449
Fragments arranged by size:
1646 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 1865 1865
Size: 4622
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 2328 3186 2328
Size: 858 3764
EaeI y'GGCC_r
Cuts at: 134 364 2207 2381 2772 2799 4144 134
Size: 230 1843 174 391 27 1345 612
Fragments arranged by size:
1843 1345 612 391 230 174 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3140 3286 4432 2830
Size: 310 146 1146 3020
Fragments arranged by size:
3020 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1634 1634
Size: 4622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 716 881 965 1217 1385 1469 2447
Size: 123 421 165 84 252 168 84 978
Cuts at: 2447 2879 3626 3894 172
Size: 432 747 268 900
Fragments arranged by size:
978 900 747 432 421 268 252 168
165 123 84 84
EcoO109I rG'GnC_Cy
Cuts at: 1761 1761
Size: 4622
EcoRI G'AATT_C
Cuts at: 142 851 935 1103 1355 1439 1626 142
Size: 709 84 168 252 84 187 3138
Fragments arranged by size:
3138 709 252 187 168 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 626 2641 626
Size: 2015 2607
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 2056 2304 3326 4305 2056
Size: 248 1022 979 2373
Fragments arranged by size:
2373 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 2464 2494 2496 2526 255
Size: 32 2177 30 2 30 2351
Fragments arranged by size:
2351 2177 32 30 30 2
HincII GTy'rAC
Cuts at: 119 1661 119
Size: 1542 3080
HindIII A'AGCT_T
Cuts at: 1064 1148 1232 1484 1064
Size: 84 84 252 4202
Fragments arranged by size:
4202 252 84 84
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3293 3477 3293
Size: 184 4438
MscI TGG'CCA
Cuts at: 136 366 2383 136
Size: 230 2017 2375
Fragments arranged by size:
2375 2017 230
MslI CAynn'nnrTG
Cuts at: 1044 2611 2738 1044
Size: 1567 127 2928
Fragments arranged by size:
2928 1567 127
MspA1I CmG'CkG
Cuts at: 1733 2047 2407 3420 3665 1733
Size: 314 360 1013 245 2690
Fragments arranged by size:
2690 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NspI r_CATG'y
Cuts at: 1675 2175 3082 1675
Size: 500 907 3215
Fragments arranged by size:
3215 907 500
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4590
PsiI TTA'TAA
Cuts at: 497 497
Size: 4622
PstI C_TGCA'G
Cuts at: 1669 2354 1669
Size: 685 3937
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 2047 2407 2047
Size: 360 4262
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 1636 1636
Size: 4622
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 4622
SfcI C'TryA_G
Cuts at: 33 216 1280 1665 2350 3343 3534 33
Size: 183 1064 385 685 993 191 1121
Fragments arranged by size:
1121 1064 993 685 385 191 183
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 1644 1644
Size: 4622
SmlI C'TyrA_G
Cuts at: 1777 2692 3184 3446 1777
Size: 915 492 262 2953
Fragments arranged by size:
2953 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
SspI AAT'ATT
Cuts at: 539 539
Size: 4622
StyI C'CwwG_G
Cuts at: 107 292 1756 2110 107
Size: 185 1464 354 2619
Fragments arranged by size:
2619 1464 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2205 2338 2797 3006 2205
Size: 133 459 209 3821
Fragments arranged by size:
3821 459 209 133
TatI w'GTAC_w
Cuts at: 754 4137 754
Size: 3383 1239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4590
Tth111I GACn'n_nGTC
Cuts at: 2419 2419
Size: 4622
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2117
Size: 4622
XmaI C'CCGG_G
Cuts at: 1642 1642
Size: 4622
XmnI GAAnn'nnTTC
Cuts at: 622 622
Size: 4622
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI