Restriction map for
pF1KB7763
(Circular) (Six-base) MAPSORT of: pF1KB7763.seq Check: 6137 from: 1 to: 4457
pF1KB7763 4457 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 277 277
Size: 4457
AccI GT'mk_AC
Cuts at: 317 1495 317
Size: 1178 3279
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 178 4297 178
Size: 4119 338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 2282 2714 3461 3729 436
Size: 1846 432 747 268 1164
Fragments arranged by size:
1846 1164 747 432 268
AflII C'TTAA_G
Cuts at: 1267 1267
Size: 4457
AflIII A'CryG_T
Cuts at: 1809 2913 3890 1809
Size: 1104 977 2376
Fragments arranged by size:
2376 1104 977
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AleI CACnn'nnGTG
Cuts at: 775 931 775
Size: 156 4301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 356 356
Size: 4457
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1013 1045 1013
Size: 32 4425
AlwNI CAG_nnn'CTG
Cuts at: 1762 3329 1762
Size: 1567 2890
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 511 762 870 1461 3810 4066 511
Size: 251 108 591 2349 256 902
Fragments arranged by size:
2349 902 591 256 251 108
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 1477 1477
Size: 4457
BamHI G'GATC_C
Cuts at: 1482 1482
Size: 4457
BanI G'GyrC_C
Cuts at: 193 1473 1887 2135 2170 193
Size: 1280 414 248 35 2480
Fragments arranged by size:
2480 1280 414 248 35
BanII G_rGCy'C
Cuts at: 1212 1471 2501 1212
Size: 259 1030 3168
Fragments arranged by size:
3168 1030 259
BbeI G_GCGC'C
Cuts at: 197 1891 2139 197
Size: 1694 248 2515
Fragments arranged by size:
2515 1694 248
BbsI GAAGACnn'nnnn_
Cuts at: 1135 1135
Size: 4457
BbvCI CC'TCA_GC
Cuts at: 803 1334 803
Size: 531 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 337 371 337
Size: 34 4423
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 1977 1977
Size: 4457
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 1569 1569
Size: 4457
Bme1580I G_kGCm'C
Cuts at: 2082 2175 3231 2082
Size: 93 1056 3308
Fragments arranged by size:
3308 1056 93
BmgBI CAC'GTC
Cuts at: 668 668
Size: 4457
BmrI ACTGGGnnnn_n'
Cuts at: 1403 1669 1750 2085 4229 1403
Size: 266 81 335 2144 1631
Fragments arranged by size:
2144 1631 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 562 1001 1174 1294 559
Size: 3 439 173 120 3722
Fragments arranged by size:
3722 439 173 120 3
Bpu10I CC'TnA_GC
Cuts at: 803 1334 803
Size: 531 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2512 3004 3302 1633
Size: 879 492 298 2788
Fragments arranged by size:
2788 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4161 4161
Size: 4457
BsaAI yAC'GTr
Cuts at: 1810 1810
Size: 4457
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 194 1888 2136 2838 4334 194
Size: 1694 248 702 1496 317
Fragments arranged by size:
1694 1496 702 317 248
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3119 3266 3677 1646
Size: 521 678 274 147 411 2426
Fragments arranged by size:
2426 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 479 509 1214 1244 479
Size: 30 705 30 3692
Fragments arranged by size:
3692 705 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1075 1075
Size: 4457
BseYI C'CCAG_C
Cuts at: 1882 2640 3217 3713 1882
Size: 758 577 496 2626
Fragments arranged by size:
2626 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 4457
BsiEI CG_ry'CG
Cuts at: 104 1999 2045 3253 104
Size: 1895 46 1208 1308
Fragments arranged by size:
1895 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1471 2249 2439 3231 1471
Size: 778 190 792 2697
Fragments arranged by size:
2697 792 778 190
BsmI GAATG_Cn'
Cuts at: 405 405
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 1212 1471 2082 2175 2249 2439 2501 3231 1212
Size: 259 611 93 74 190 62 730 2438
Fragments arranged by size:
2438 730 611 259 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 3633 3633
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 277 1050 1507 1721 2023 2404 277
Size: 773 457 214 302 381 2330
Fragments arranged by size:
2330 773 457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2749 2803 4287 4452 50
Size: 2699 54 1484 165 55
Fragments arranged by size:
2699 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 1167 2369 590
Size: 577 1202 2678
Fragments arranged by size:
2678 1202 577
BsrFI r'CCGG_y
Cuts at: 2455 2636 2845 2455
Size: 181 209 4067
Fragments arranged by size:
4067 209 181
BssSI C'ACGA_G
Cuts at: 2728 3086 2728
Size: 358 4099
BstAPI GCAn_nnn'nTGC
Cuts at: 799 1834 799
Size: 1035 3422
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstEII G'GTnAC_C
Cuts at: 730 730
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4457
BstYI r'GATC_y
Cuts at: 1 1482 1949 1972 2307 2553 3565 3651 3663
Size: 1481 467 23 335 246 1012 86 12
Cuts at: 3663 4009 1
Size: 346 449
Fragments arranged by size:
1481 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 726 726
Size: 4457
BtgI C'CryG_G
Cuts at: 1316 1433 1316
Size: 117 4340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 1700 1700
Size: 4457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1121 1156 1121
Size: 35 4422
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DraIII CAC_nnn'GTG
Cuts at: 366 366
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3021 2163
Size: 858 3599
EaeI y'GGCC_r
Cuts at: 990 2042 2216 2607 2634 3979 990
Size: 1052 174 391 27 1345 1468
Fragments arranged by size:
1468 1345 1052 391 174 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 1082 1200 1319 1367 2480 2690 1082
Size: 118 119 48 1113 210 2849
Fragments arranged by size:
2849 1113 210 119 118 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2665 2975 3121 4267 2665
Size: 310 146 1146 2855
Fragments arranged by size:
2855 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1469 1469
Size: 4457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 559 562 1001 1174 1294 2282 2714 3461
Size: 123 3 439 173 120 988 432 747
Cuts at: 3461 3729 436
Size: 268 1164
Fragments arranged by size:
1164 988 747 439 432 268 173 123
120 3
EcoNI CCTnn'n_nnAGG
Cuts at: 980 980
Size: 4457
EcoO109I rG'GnC_Cy
Cuts at: 323 1596 323
Size: 1273 3184
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 295 2476 295
Size: 2181 2276
FspI TGC'GCA
Cuts at: 359 2238 359
Size: 1879 2578
FspAI rTGC'GCAy
Cuts at: 359 359
Size: 4457
HaeII r_GCGC'y
Cuts at: 197 450 1891 2139 3161 4140 197
Size: 253 1441 248 1022 979 514
Fragments arranged by size:
1441 1022 979 514 253 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 1214 1246 2299 2329 2331 2361 479
Size: 32 703 32 1053 30 2 30 2575
Fragments arranged by size:
2575 1053 703 32 32 30 30 2
HincII GTy'rAC
Cuts at: 684 879 1496 684
Size: 195 617 3645
Fragments arranged by size:
3645 617 195
HpaI GTT'AAC
Cuts at: 684 684
Size: 4457
KasI G'GCGC_C
Cuts at: 193 1887 2135 193
Size: 1694 248 2515
Fragments arranged by size:
2515 1694 248
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 848 1349 3128 3312 848
Size: 501 1779 184 1993
Fragments arranged by size:
1993 1779 501 184
MscI TGG'CCA
Cuts at: 992 2218 992
Size: 1226 3231
MslI CAynn'nnrTG
Cuts at: 458 775 931 2446 2573 458
Size: 317 156 1515 127 2342
Fragments arranged by size:
2342 1515 317 156 127
MspA1I CmG'CkG
Cuts at: 1568 1882 2242 3255 3500 1568
Size: 314 360 1013 245 2525
Fragments arranged by size:
2525 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 4457
NarI GG'CG_CC
Cuts at: 194 1888 2136 194
Size: 1694 248 2515
Fragments arranged by size:
2515 1694 248
NcoI C'CATG_G
Cuts at: 1316 1433 1316
Size: 117 4340
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 4457
NspI r_CATG'y
Cuts at: 358 414 1510 2010 2917 358
Size: 56 1096 500 907 1898
Fragments arranged by size:
1898 1096 907 500 56
PciI A'CATG_T
Cuts at: 2913 2913
Size: 4457
PflMI CCAn_nnn'nTGG
Cuts at: 212 212
Size: 4457
PfoI T'CCnGG_A
Cuts at: 575 1190 575
Size: 615 3842
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4425
PpuMI rG'GwC_Cy
Cuts at: 323 323
Size: 4457
PstI C_TGCA'G
Cuts at: 345 1504 2189 345
Size: 1159 685 2613
Fragments arranged by size:
2613 1159 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 1882 2242 1882
Size: 360 4097
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 1471 1471
Size: 4457
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 1200 2480 2690 1200
Size: 1280 210 2967
Fragments arranged by size:
2967 1280 210
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 275 3974 275
Size: 3699 758
SfcI C'TryA_G
Cuts at: 33 341 1177 1346 1500 2185 3178 3369 33
Size: 308 836 169 154 685 993 191 1121
Fragments arranged by size:
1121 993 836 685 308 191 169 154
SfoI GGC'GCC
Cuts at: 195 1889 2137 195
Size: 1694 248 2515
Fragments arranged by size:
2515 1694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 1267 1612 2527 3019 3281 1267
Size: 345 915 492 262 2443
Fragments arranged by size:
2443 915 492 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1510 2010 1510
Size: 500 3957
StyI C'CwwG_G
Cuts at: 404 1114 1316 1433 1591 1945 404
Size: 710 202 117 158 354 2916
Fragments arranged by size:
2916 710 354 202 158 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2040 2173 2632 2841 2040
Size: 133 459 209 3656
Fragments arranged by size:
3656 459 209 133
TatI w'GTAC_w
Cuts at: 273 750 3972 273
Size: 477 3222 758
Fragments arranged by size:
3222 758 477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4425
Tth111I GACn'n_nGTC
Cuts at: 1395 2254 1395
Size: 859 3598
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 777 1952 114
Size: 663 1175 2619
Fragments arranged by size:
2619 1175 663
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI ApaI AscI AvrII BaeI BfrBI
BglI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
EcoRV FseI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI