Restriction map for
pF1KB7755
(Circular) (Six-base) MAPSORT of: pF1KB7755.seq Check: 6617 from: 1 to: 4451
pF1KB7755 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 318 494 318
Size: 176 4275
AatII G_ACGT'C
Cuts at: 953 953
Size: 4451
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 859 873 2276 2708 3455 3723 740
Size: 119 14 1403 432 747 268 1468
Fragments arranged by size:
1468 1403 747 432 268 119 14
AfeI AGC'GCT
Cuts at: 295 295
Size: 4451
AflIII A'CryG_T
Cuts at: 1803 2907 3884 1803
Size: 1104 977 2370
Fragments arranged by size:
2370 1104 977
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AhdI GACnn_n'nnGTC
Cuts at: 321 321
Size: 4451
AleI CACnn'nnGTG
Cuts at: 1064 1064
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 972 1409 1756 3323 972
Size: 437 347 1567 2100
Fragments arranged by size:
2100 1567 437 347
ApaI G_GGCC'C
Cuts at: 1059 1059
Size: 4451
ApaLI G'TGCA_C
Cuts at: 3221 3221
Size: 4451
ApoI r'AATT_y
Cuts at: 1455 3804 4060 1455
Size: 2349 256 1846
Fragments arranged by size:
2349 1846 256
AscI GG'CGCG_CC
Cuts at: 454 454
Size: 4451
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 263 669 997 1163 1471 263
Size: 406 328 166 308 3243
Fragments arranged by size:
3243 406 328 308 166
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 157 259 357 703 740 823 872 931 1021
Size: 102 98 346 37 83 49 59 90
Cuts at: 1021 1285 1467 1881 2129 2164 157
Size: 264 182 414 248 35 2444
Fragments arranged by size:
2444 414 346 264 248 182 102 98
90 83 59 49 37 35
BanII G_rGCy'C
Cuts at: 503 914 1059 1141 1465 2495 503
Size: 411 145 82 324 1030 2459
Fragments arranged by size:
2459 1030 411 324 145 82
BbeI G_GCGC'C
Cuts at: 263 827 876 1025 1885 2133 263
Size: 564 49 149 860 248 2581
Fragments arranged by size:
2581 860 564 248 149 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4417
BciVI GTATCCnnnnn_n'
Cuts at: 2349 3110 4451 2349
Size: 761 1341 2349
Fragments arranged by size:
2349 1341 761
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglI GCCn_nnn'nGGC
Cuts at: 155 1075 1151 1213 1344 155
Size: 920 76 62 131 3262
Fragments arranged by size:
3262 920 131 76 62
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 502 535 913 1563 502
Size: 33 378 650 3390
Fragments arranged by size:
3390 650 378 33
Bme1580I G_kGCm'C
Cuts at: 362 743 934 1059 1071 2076 2169 3225 362
Size: 381 191 125 12 1005 93 1056 1588
Fragments arranged by size:
1588 1056 1005 381 191 125 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 1419 1663 1744 2079 4223 1419
Size: 244 81 335 2144 1647
Fragments arranged by size:
2144 1647 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 2818 147
Size: 2671 1780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 865 1257 369
Size: 496 392 3563
Fragments arranged by size:
3563 496 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1627 2506 2998 3296 319
Size: 1308 879 492 298 1474
Fragments arranged by size:
1474 1308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4155 4155
Size: 4451
BsaAI yAC'GTr
Cuts at: 180 1804 180
Size: 1624 2827
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 260 317 824 852 873 941 950 1022 1042
Size: 57 507 28 21 68 9 72 20
Cuts at: 1042 1882 2130 2832 4328 260
Size: 840 248 702 1496 383
Fragments arranged by size:
1496 840 702 507 383 248 72 68
57 28 21 20 9
BsaWI w'CCGG_w
Cuts at: 1640 2161 2839 3113 3260 3671 1640
Size: 521 678 274 147 411 2420
Fragments arranged by size:
2420 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 4421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 4451
BseYI C'CCAG_C
Cuts at: 134 513 681 1876 2634 3211 3707 134
Size: 379 168 1195 758 577 496 878
Fragments arranged by size:
1195 878 758 577 496 379 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 607 472
Size: 135 4316
BsiEI CG_ry'CG
Cuts at: 104 859 1114 1217 1993 2039 3247 104
Size: 755 255 103 776 46 1208 1308
Fragments arranged by size:
1308 1208 776 755 255 103 46
BsiHKAI G_wGCw'C
Cuts at: 335 503 914 1465 2243 2433 3225 335
Size: 168 411 551 778 190 792 1561
Fragments arranged by size:
1561 792 778 551 411 190 168
BsmI GAATG_Cn'
Cuts at: 430 430
Size: 4451
BsmBI CGTCTCn'nnnn_
Cuts at: 215 1090 215
Size: 875 3576
Bsp1286I G_dGCh'C
Cuts at: 335 362 503 743 914 934 1059 1071 1141
Size: 27 141 240 171 20 125 12 70
Cuts at: 1141 1465 2076 2169 2243 2433 2495 3225 335
Size: 324 611 93 74 190 62 730 1561
Fragments arranged by size:
1561 730 611 324 240 190 171 141
125 93 74 70 62 27 20 12
BspEI T'CCGG_A
Cuts at: 3671 3671
Size: 4451
BspHI T'CATG_A
Cuts at: 3627 3627
Size: 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 494 980 1271 1501 1715 2017 2398 318
Size: 176 486 291 230 214 302 381 2371
Fragments arranged by size:
2371 486 381 302 291 230 214 176
BsrBI CCG'CTC
Cuts at: 50 131 2743 2797 4281 4446 50
Size: 81 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2363 2363
Size: 4451
BsrFI r'CCGG_y
Cuts at: 217 366 649 854 858 1225 1237 1343 1419
Size: 149 283 205 4 367 12 106 76
Cuts at: 1419 2449 2630 2839 217
Size: 1030 181 209 1829
Fragments arranged by size:
1829 1030 367 283 209 205 181 149
106 76 12 4
BsrGI T'GTAC_A
Cuts at: 710 1424 710
Size: 714 3737
BssHII G'CGCG_C
Cuts at: 389 454 389
Size: 65 4386
BssSI C'ACGA_G
Cuts at: 955 2722 3080 955
Size: 1767 358 2326
Fragments arranged by size:
2326 1767 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1828 1828
Size: 4451
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstYI r'GATC_y
Cuts at: 1 1476 1943 1966 2301 2547 3559 3645 3657
Size: 1475 467 23 335 246 1012 86 12
Cuts at: 3657 4003 1
Size: 346 449
Fragments arranged by size:
1475 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 625 747 771 935 1108 625
Size: 122 24 164 173 3968
Fragments arranged by size:
3968 173 164 122 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2401 2582 2745 2401
Size: 181 163 4107
Fragments arranged by size:
4107 181 163
BtsI GCAGTG_nn'
Cuts at: 1694 1694
Size: 4451
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 467 651 856 1111 1143 1194 1214 2036 2210
Size: 184 205 255 32 51 20 822 174
Cuts at: 2210 2601 2628 3973 467
Size: 391 27 1345 945
Fragments arranged by size:
1345 945 822 391 255 205 184 174
51 32 27 20
EagI C'GGCC_G
Cuts at: 856 1111 1214 2036 856
Size: 255 103 822 3271
Fragments arranged by size:
3271 822 255 103
EarI CTCTTCn'nnn_
Cuts at: 631 2474 2684 631
Size: 1843 210 2398
Fragments arranged by size:
2398 1843 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 301 2659 2969 3115 4261 292
Size: 9 2358 310 146 1146 482
Fragments arranged by size:
2358 1146 482 310 146 9
EcoICRI GAG'CTC
Cuts at: 501 912 1463 501
Size: 411 551 3489
Fragments arranged by size:
3489 551 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 740 859 865 873 1257 2276 2708 3455
Size: 371 119 6 8 384 1019 432 747
Cuts at: 3455 3723 369
Size: 268 1097
Fragments arranged by size:
1097 1019 747 432 384 371 268 119
8 6
EcoO109I rG'GnC_Cy
Cuts at: 228 685 1102 1590 228
Size: 457 417 488 3089
Fragments arranged by size:
3089 488 457 417
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 573 745 2470 573
Size: 172 1725 2554
Fragments arranged by size:
2554 1725 172
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 263 297 531 579 644 827 876 1025 1208
Size: 34 234 48 65 183 49 149 183
Cuts at: 1208 1885 2133 3155 4134 263
Size: 677 248 1022 979 580
Fragments arranged by size:
1022 979 677 580 248 234 183 183
149 65 49 48 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 307 339 652 684 2293 2323 2325
Size: 32 97 32 313 32 1609 30 2
Cuts at: 2325 2355 178
Size: 30 2274
Fragments arranged by size:
2274 1609 313 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
KasI G'GCGC_C
Cuts at: 259 823 872 1021 1881 2129 259
Size: 564 49 149 860 248 2581
Fragments arranged by size:
2581 860 564 248 149 49
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 3122 3306 689
Size: 2433 184 1834
Fragments arranged by size:
2433 1834 184
MscI TGG'CCA
Cuts at: 2212 2212
Size: 4451
MslI CAynn'nnrTG
Cuts at: 367 746 1064 2440 2567 367
Size: 379 318 1376 127 2251
Fragments arranged by size:
2251 1376 379 318 127
MspA1I CmG'CkG
Cuts at: 869 1193 1562 1876 2236 3249 3494 869
Size: 324 369 314 360 1013 245 1826
Fragments arranged by size:
1826 1013 369 360 324 314 245
NaeI GCC'GGC
Cuts at: 219 651 856 1227 1239 1345 2632 219
Size: 432 205 371 12 106 1287 2038
Fragments arranged by size:
2038 1287 432 371 205 106 12
NarI GG'CG_CC
Cuts at: 260 824 873 1022 1882 2130 260
Size: 564 49 149 860 248 2581
Fragments arranged by size:
2581 860 564 248 149 49
NcoI C'CATG_G
Cuts at: 747 935 747
Size: 188 4263
NgoMIV G'CCGG_C
Cuts at: 217 649 854 1225 1237 1343 2630 217
Size: 432 205 371 12 106 1287 2038
Fragments arranged by size:
2038 1287 432 371 205 106 12
NspI r_CATG'y
Cuts at: 412 1504 2004 2911 412
Size: 1092 500 907 1952
Fragments arranged by size:
1952 1092 907 500
PasI CC'CwG_GG
Cuts at: 978 978
Size: 4451
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PflMI CCAn_nnn'nTGG
Cuts at: 1409 1409
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PmlI CAC'GTG
Cuts at: 180 180
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PpuMI rG'GwC_Cy
Cuts at: 685 1102 685
Size: 417 4034
PshAI GACnn'nnGTC
Cuts at: 350 350
Size: 4451
PspOMI G'GGCC_C
Cuts at: 1055 1055
Size: 4451
PstI C_TGCA'G
Cuts at: 148 815 1192 1268 1498 2183 148
Size: 667 377 76 230 685 2416
Fragments arranged by size:
2416 685 667 377 230 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 1876 2236 1876
Size: 360 4091
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 503 914 1465 503
Size: 411 551 3489
Fragments arranged by size:
3489 551 411
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SanDI GG'GwC_CC
Cuts at: 685 1102 685
Size: 417 4034
SapI GCTCTTCn'nnn_
Cuts at: 631 2474 2684 631
Size: 1843 210 2398
Fragments arranged by size:
2398 1843 210
SbfI CC_TGCA'GG
Cuts at: 1268 1498 1268
Size: 230 4221
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 144 811 1188 1264 1494 2179 3172 3363
Size: 111 667 377 76 230 685 993 191
Cuts at: 3363 33
Size: 1121
Fragments arranged by size:
1121 993 685 667 377 230 191 111
76
SfoI GGC'GCC
Cuts at: 261 825 874 1023 1883 2131 261
Size: 564 49 149 860 248 2581
Fragments arranged by size:
2581 860 564 248 149 49
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 265 1473 265
Size: 1208 3243
SmlI C'TyrA_G
Cuts at: 334 1606 2521 3013 3275 334
Size: 1272 915 492 262 1510
Fragments arranged by size:
1510 1272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StuI AGG'CCT
Cuts at: 1012 1270 1012
Size: 258 4193
StyI C'CwwG_G
Cuts at: 747 935 1585 1939 747
Size: 188 650 354 3259
Fragments arranged by size:
3259 650 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2034 2167 2626 2835 2034
Size: 133 459 209 3650
Fragments arranged by size:
3650 459 209 133
TatI w'GTAC_w
Cuts at: 710 1424 3966 710
Size: 714 2542 1195
Fragments arranged by size:
2542 1195 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 3968 4000 646
Size: 32 3290 32 1097
Fragments arranged by size:
3290 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 1946 177
Size: 1769 2682
XmaI C'CCGG_G
Cuts at: 263 1471 263
Size: 1208 3243
XmnI GAAnn'nnTTC
Cuts at: 430 430
Size: 4451
ZraI GAC'GTC
Cuts at: 951 951
Size: 4451
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AloI AvrII BaeI BbsI BbvCI BfrBI
BmgBI BmtI Bpu10I BsiWI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI