Restriction map for
pF1KB7750
(Circular) (Six-base) MAPSORT of: pF1KB7750.seq Check: 4727 from: 1 to: 4517
pF1KB7750 4517 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 346 447 2342 2774 3521 3789 196
Size: 150 101 1895 432 747 268 924
Fragments arranged by size:
1895 924 747 432 268 150 101
AflIII A'CryG_T
Cuts at: 744 746 1869 2973 3950 744
Size: 2 1123 1104 977 1311
Fragments arranged by size:
1311 1123 1104 977 2
AgeI A'CCGG_T
Cuts at: 2905 2905
Size: 4517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 4517
AlwNI CAG_nnn'CTG
Cuts at: 375 1822 3389 375
Size: 1447 1567 1503
Fragments arranged by size:
1567 1503 1447
ApaLI G'TGCA_C
Cuts at: 3287 3287
Size: 4517
ApoI r'AATT_y
Cuts at: 1521 3870 4126 1521
Size: 2349 256 1912
Fragments arranged by size:
2349 1912 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 1378 1537 1378
Size: 159 4358
BamHI G'GATC_C
Cuts at: 1436 1542 1436
Size: 106 4411
BanI G'GyrC_C
Cuts at: 1533 1947 2195 2230 1533
Size: 414 248 35 3820
Fragments arranged by size:
3820 414 248 35
BanII G_rGCy'C
Cuts at: 843 1210 1256 1531 2561 843
Size: 367 46 275 1030 2799
Fragments arranged by size:
2799 1030 367 275 46
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 224 224
Size: 4517
BbvCI CC'TCA_GC
Cuts at: 1122 1122
Size: 4517
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3176 4517 2415
Size: 761 1341 2415
Fragments arranged by size:
2415 1341 761
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BglI GCCn_nnn'nGGC
Cuts at: 1166 1166
Size: 4517
BglII A'GATC_T
Cuts at: 2032 4069 2032
Size: 2037 2480
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 2142 2235 3291 2142
Size: 93 1056 3368
Fragments arranged by size:
3368 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 122 584 1009 1729 1810 2145 4289 122
Size: 462 425 720 81 335 2144 350
Fragments arranged by size:
2144 720 462 425 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 862 2884 862
Size: 2022 2495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 842 147
Size: 695 3822
Bpu10I CC'TnA_GC
Cuts at: 147 889 1122 147
Size: 742 233 3542
Fragments arranged by size:
3542 742 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2572 3064 3362 1693
Size: 879 492 298 2848
Fragments arranged by size:
2848 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1022 4221 1022
Size: 3199 1318
BsaAI yAC'GTr
Cuts at: 747 1870 747
Size: 1123 3394
BsaBI GATnn'nnATC
Cuts at: 6 2031 4068 6
Size: 2025 2037 455
Fragments arranged by size:
2037 2025 455
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1439 1706 2227 2905 3179 3326 3737 1193
Size: 246 267 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 267 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 636 666 869 899 1090 1120 636
Size: 30 203 30 191 30 4033
Fragments arranged by size:
4033 203 191 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 657 1098 1101 1104 1107 1110 1408 657
Size: 441 3 3 3 3 298 3766
Fragments arranged by size:
3766 441 298 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1210 1289 1333 1942 2700 3277 3773 1210
Size: 79 44 609 758 577 496 1954
Fragments arranged by size:
1954 758 609 577 496 79 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1408 289
Size: 1119 3398
BsiEI CG_ry'CG
Cuts at: 104 919 2059 2105 3313 104
Size: 815 1140 46 1208 1308
Fragments arranged by size:
1308 1208 1140 815 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1531 2309 2499 3291 1084
Size: 447 778 190 792 2310
Fragments arranged by size:
2310 792 778 447 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1353 1353
Size: 4517
Bsp1286I G_dGCh'C
Cuts at: 843 1084 1210 1256 1531 2142 2235 2309 2499
Size: 241 126 46 275 611 93 74 190
Cuts at: 2499 2561 3291 843
Size: 62 730 2069
Fragments arranged by size:
2069 730 611 275 241 190 126 93
74 62 46
BspEI T'CCGG_A
Cuts at: 1193 3737 1193
Size: 2544 1973
BspHI T'CATG_A
Cuts at: 3693 3693
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1567 1781 2083 2464 1567
Size: 214 302 381 3620
Fragments arranged by size:
3620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2429 2429
Size: 4517
BsrFI r'CCGG_y
Cuts at: 2515 2696 2905 2515
Size: 181 209 4127
Fragments arranged by size:
4127 209 181
BssSI C'ACGA_G
Cuts at: 307 1201 2788 3146 307
Size: 894 1587 358 1678
Fragments arranged by size:
1678 1587 894 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 1894
Size: 4517
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 806 806
Size: 4517
BstYI r'GATC_y
Cuts at: 1 536 1436 1542 2009 2032 2367 2613 3625
Size: 535 900 106 467 23 335 246 1012
Cuts at: 3625 3711 3723 4069 1
Size: 86 12 346 449
Fragments arranged by size:
1012 900 535 467 449 346 335 246
106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 349 773 349
Size: 424 4093
BtgI C'CryG_G
Cuts at: 107 269 107
Size: 162 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1400 2467 2648 2811 1400
Size: 1067 181 163 3106
Fragments arranged by size:
3106 1067 181 163
BtsI GCAGTG_nn'
Cuts at: 1062 1342 1760 1062
Size: 280 418 3819
Fragments arranged by size:
3819 418 280
DraI TTT'AAA
Cuts at: 1516 1516
Size: 4517
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3081 2223
Size: 858 3659
EaeI y'GGCC_r
Cuts at: 482 808 916 950 2102 2276 2667 2694 4039
Size: 326 108 34 1152 174 391 27 1345
Cuts at: 4039 482
Size: 960
Fragments arranged by size:
1345 1152 960 391 326 174 108 34
27
EagI C'GGCC_G
Cuts at: 916 2102 916
Size: 1186 3331
EarI CTCTTCn'nnn_
Cuts at: 254 275 322 1217 2540 2750 254
Size: 21 47 895 1323 210 2021
Fragments arranged by size:
2021 1323 895 210 47 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2725 3035 3181 4327 2725
Size: 310 146 1146 2915
Fragments arranged by size:
2915 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1529 1529
Size: 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 196 346 447 842 2342 2774 3521 3789
Size: 49 150 101 395 1500 432 747 268
Cuts at: 3789 147
Size: 875
Fragments arranged by size:
1500 875 747 432 395 268 150 101
49
EcoO109I rG'GnC_Cy
Cuts at: 207 372 885 1329 1415 1656 207
Size: 165 513 444 86 241 3068
Fragments arranged by size:
3068 513 444 241 165 86
EcoRI G'AATT_C
Cuts at: 1521 1521
Size: 4517
EcoRV GAT'ATC
Cuts at: 525 525
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4517
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 510 1305 1951 2199 3221 4200 510
Size: 795 646 248 1022 979 827
Fragments arranged by size:
1022 979 827 795 646 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 714 746 869 901 1090 1122 2359 2389 2391
Size: 32 123 32 189 32 1237 30 2
Cuts at: 2391 2421 714
Size: 30 2810
Fragments arranged by size:
2810 1237 189 123 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 523 1094 3188 3372 523
Size: 571 2094 184 1668
Fragments arranged by size:
2094 1668 571 184
MscI TGG'CCA
Cuts at: 484 2278 484
Size: 1794 2723
MslI CAynn'nnrTG
Cuts at: 478 940 2506 2633 478
Size: 462 1566 127 2362
Fragments arranged by size:
2362 1566 462 127
MspA1I CmG'CkG
Cuts at: 194 467 921 949 974 1025 1277 1628 1942
Size: 273 454 28 25 51 252 351 314
Cuts at: 1942 2302 3315 3560 194
Size: 360 1013 245 1151
Fragments arranged by size:
1151 1013 454 360 351 314 273 252
245 51 28 25
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 4517
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 107 269 107
Size: 162 4355
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 4517
NotI GC'GGCC_GC
Cuts at: 916 916
Size: 4517
NspI r_CATG'y
Cuts at: 417 1570 2070 2977 417
Size: 1153 500 907 1957
Fragments arranged by size:
1957 1153 907 500
PciI A'CATG_T
Cuts at: 2973 2973
Size: 4517
PflMI CCAn_nnn'nTGG
Cuts at: 375 1006 375
Size: 631 3886
PfoI T'CCnGG_A
Cuts at: 368 858 368
Size: 490 4027
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PmlI CAC'GTG
Cuts at: 747 747
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4485
PpuMI rG'GwC_Cy
Cuts at: 372 885 1329 1415 372
Size: 513 444 86 3474
Fragments arranged by size:
3474 513 444 86
PstI C_TGCA'G
Cuts at: 1564 2249 1564
Size: 685 3832
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 467 974 1942 2302 467
Size: 507 968 360 2682
Fragments arranged by size:
2682 968 507 360
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 1531 1531
Size: 4517
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SanDI GG'GwC_CC
Cuts at: 1415 1415
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 275 322 2540 2750 275
Size: 47 2218 210 2042
Fragments arranged by size:
2218 2042 210 47
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 1050 1560 2245 3238 3429 33
Size: 1017 510 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 510 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 1672 2587 3079 3341 1672
Size: 915 492 262 2848
Fragments arranged by size:
2848 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
StyI C'CwwG_G
Cuts at: 107 203 269 1034 1151 1256 1316 1651 2005
Size: 96 66 765 117 105 60 335 354
Cuts at: 2005 107
Size: 2619
Fragments arranged by size:
2619 765 354 335 117 105 96 66
60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 333 566 749 755 816 2100 2233 2692 2901
Size: 233 183 6 61 1284 133 459 209
Cuts at: 2901 333
Size: 1949
Fragments arranged by size:
1949 1284 459 233 209 183 133 61
6
TatI w'GTAC_w
Cuts at: 4032 4032
Size: 4517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 780 812 978 1010 4034 4066 780
Size: 32 166 32 3024 32 1231
Fragments arranged by size:
3024 1231 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 173 2314 173
Size: 2141 2376
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 2012 1263
Size: 749 3768
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
XmnI GAAnn'nnTTC
Cuts at: 548 548
Size: 4517
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI