Restriction map for
pF1KB7749
(Circular) (Six-base) MAPSORT of: pF1KB7749.seq Check: 4508 from: 1 to: 4442
pF1KB7749 4442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1480 1480
Size: 4442
Acc65I G'GTAC_C
Cuts at: 1015 1458 1015
Size: 443 3999
AclI AA'CG_TT
Cuts at: 4282 4282
Size: 4442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2699 3446 3714 2267
Size: 432 747 268 2995
Fragments arranged by size:
2995 747 432 268
AfeI AGC'GCT
Cuts at: 1090 1090
Size: 4442
AflIII A'CryG_T
Cuts at: 1794 2898 3875 1794
Size: 1104 977 2361
Fragments arranged by size:
2361 1104 977
AgeI A'CCGG_T
Cuts at: 2830 2830
Size: 4442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 886 928 886
Size: 42 4400
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 718 750 718
Size: 32 4410
AlwNI CAG_nnn'CTG
Cuts at: 1747 3314 1747
Size: 1567 2875
ApaLI G'TGCA_C
Cuts at: 3212 3212
Size: 4442
ApoI r'AATT_y
Cuts at: 1406 1446 3795 4051 1406
Size: 40 2349 256 1797
Fragments arranged by size:
2349 1797 256 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
AvaI C'yCGr_G
Cuts at: 1284 1462 1284
Size: 178 4264
BamHI G'GATC_C
Cuts at: 1467 1467
Size: 4442
BanI G'GyrC_C
Cuts at: 226 1015 1120 1288 1306 1458 1872 2120 2155
Size: 789 105 168 18 152 414 248 35
Cuts at: 2155 226
Size: 2513
Fragments arranged by size:
2513 789 414 248 168 152 105 35
18
BanII G_rGCy'C
Cuts at: 538 661 980 1229 1456 2486 538
Size: 123 319 249 227 1030 2494
Fragments arranged by size:
2494 1030 319 249 227 123
BbeI G_GCGC'C
Cuts at: 230 1292 1876 2124 230
Size: 1062 584 248 2548
Fragments arranged by size:
2548 1062 584 248
BbsI GAAGACnn'nnnn_
Cuts at: 1194 1194
Size: 4442
BbvCI CC'TCA_GC
Cuts at: 422 434 422
Size: 12 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 236 270 90
Size: 34 112 34 4262
Fragments arranged by size:
4262 112 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2340 3101 4442 2340
Size: 761 1341 2340
Fragments arranged by size:
2340 1341 761
BclI T'GATC_A
Cuts at: 1962 1962
Size: 4442
BfrBI ATG'CAT
Cuts at: 948 948
Size: 4442
BglI GCCn_nnn'nGGC
Cuts at: 526 1157 526
Size: 631 3811
BglII A'GATC_T
Cuts at: 815 1957 3994 815
Size: 1142 2037 1263
Fragments arranged by size:
2037 1263 1142
BlpI GC'TnA_GC
Cuts at: 1554 1554
Size: 4442
Bme1580I G_kGCm'C
Cuts at: 917 1123 2067 2160 3216 917
Size: 206 944 93 1056 2143
Fragments arranged by size:
2143 1056 944 206 93
BmrI ACTGGGnnnn_n'
Cuts at: 975 1654 1735 2070 4214 975
Size: 679 81 335 2144 1203
Fragments arranged by size:
2144 1203 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 4442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 258 609 745 247
Size: 11 351 136 3944
Fragments arranged by size:
3944 351 136 11
Bpu10I CC'TnA_GC
Cuts at: 331 422 434 630 331
Size: 91 12 196 4143
Fragments arranged by size:
4143 196 91 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 2497 2989 3287 1618
Size: 879 492 298 2773
Fragments arranged by size:
2773 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4146 4146
Size: 4442
BsaAI yAC'GTr
Cuts at: 1795 1795
Size: 4442
BsaBI GATnn'nnATC
Cuts at: 6 814 1956 3993 6
Size: 808 1142 2037 455
Fragments arranged by size:
2037 1142 808 455
BsaHI Gr'CG_yC
Cuts at: 211 227 1289 1873 2121 2823 4319 211
Size: 16 1062 584 248 702 1496 334
Fragments arranged by size:
1496 1062 702 584 334 248 16
BsaWI w'CCGG_w
Cuts at: 1631 2152 2830 3104 3251 3662 1631
Size: 521 678 274 147 411 2411
Fragments arranged by size:
2411 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 718 748 718
Size: 30 4412
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 479 479
Size: 4442
BseYI C'CCAG_C
Cuts at: 1057 1317 1867 2625 3202 3698 1057
Size: 260 550 758 577 496 1801
Fragments arranged by size:
1801 758 577 550 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 428 361
Size: 67 4375
BsiEI CG_ry'CG
Cuts at: 104 763 1984 2030 3238 104
Size: 659 1221 46 1208 1308
Fragments arranged by size:
1308 1221 1208 659 46
BsiHKAI G_wGCw'C
Cuts at: 1456 2234 2424 3216 1456
Size: 778 190 792 2682
Fragments arranged by size:
2682 792 778 190
BsmI GAATG_Cn'
Cuts at: 501 948 501
Size: 447 3995
Bsp1286I G_dGCh'C
Cuts at: 538 661 917 980 1123 1229 1456 2067 2160
Size: 123 256 63 143 106 227 611 93
Cuts at: 2160 2234 2424 2486 3216 538
Size: 74 190 62 730 1764
Fragments arranged by size:
1764 730 611 256 227 190 143 123
106 93 74 63 62
BspEI T'CCGG_A
Cuts at: 3662 3662
Size: 4442
BspHI T'CATG_A
Cuts at: 3618 3618
Size: 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1492 1706 2008 2389 1492
Size: 214 302 381 3545
Fragments arranged by size:
3545 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2734 2788 4272 4437 50
Size: 2684 54 1484 165 55
Fragments arranged by size:
2684 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2354 2354
Size: 4442
BsrFI r'CCGG_y
Cuts at: 758 925 1381 2440 2621 2830 758
Size: 167 456 1059 181 209 2370
Fragments arranged by size:
2370 1059 456 209 181 167
BssHII G'CGCG_C
Cuts at: 684 686 688 707 684
Size: 2 2 19 4419
Fragments arranged by size:
4419 19 2 2
BssSI C'ACGA_G
Cuts at: 702 2713 3071 702
Size: 2011 358 2073
Fragments arranged by size:
2073 2011 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1819 1819
Size: 4442
BstBI TT'CG_AA
Cuts at: 2803 2803
Size: 4442
BstYI r'GATC_y
Cuts at: 1 815 1467 1934 1957 2292 2538 3550 3636
Size: 814 652 467 23 335 246 1012 86
Cuts at: 3636 3648 3994 1
Size: 12 346 449
Fragments arranged by size:
1012 814 652 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1292 1335 1292
Size: 43 4399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2392 2573 2736 2392
Size: 181 163 4098
Fragments arranged by size:
4098 181 163
BtsI GCAGTG_nn'
Cuts at: 1685 1685
Size: 4442
DraI TTT'AAA
Cuts at: 1441 1441
Size: 4442
DrdI GACnn_nn'nnGTC
Cuts at: 2148 3006 2148
Size: 858 3584
EaeI y'GGCC_r
Cuts at: 527 560 760 779 1326 1383 2027 2201 2592
Size: 33 200 19 547 57 644 174 391
Cuts at: 2592 2619 3964 527
Size: 27 1345 1005
Fragments arranged by size:
1345 1005 644 547 391 200 174 57
33 27 19
EagI C'GGCC_G
Cuts at: 760 2027 760
Size: 1267 3175
EarI CTCTTCn'nnn_
Cuts at: 480 2465 2675 480
Size: 1985 210 2247
Fragments arranged by size:
2247 1985 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 204 2650 2960 3106 4252 204
Size: 2446 310 146 1146 394
Fragments arranged by size:
2446 1146 394 310 146
EcoICRI GAG'CTC
Cuts at: 1454 1454
Size: 4442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 258 609 745 2267 2699 3446 3714 247
Size: 11 351 136 1522 432 747 268 975
Fragments arranged by size:
1522 975 747 432 351 268 136 11
EcoO109I rG'GnC_Cy
Cuts at: 1175 1345 1581 1175
Size: 170 236 4036
Fragments arranged by size:
4036 236 170
EcoRI G'AATT_C
Cuts at: 1406 1446 1406
Size: 40 4402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4442
FspI TGC'GCA
Cuts at: 325 1065 2223 325
Size: 740 1158 2544
Fragments arranged by size:
2544 1158 740
HaeII r_GCGC'y
Cuts at: 230 281 338 807 1092 1292 1876 2124 3146
Size: 51 57 469 285 200 584 248 1022
Cuts at: 3146 4125 230
Size: 979 547
Fragments arranged by size:
1022 979 584 547 469 285 248 200
57 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 768 800 967 999 2284 2314 2316 2346 768
Size: 32 167 32 1285 30 2 30 2864
Fragments arranged by size:
2864 1285 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 960 1481 960
Size: 521 3921
KasI G'GCGC_C
Cuts at: 226 1288 1872 2120 226
Size: 1062 584 248 2548
Fragments arranged by size:
2548 1062 584 248
KpnI G_GTAC'C
Cuts at: 1019 1462 1019
Size: 443 3999
MluI A'CGCG_T
Cuts at: 3875 3875
Size: 4442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3113 3297 3113
Size: 184 4258
MscI TGG'CCA
Cuts at: 562 781 2203 562
Size: 219 1422 2801
Fragments arranged by size:
2801 1422 219
MslI CAynn'nnrTG
Cuts at: 1145 2431 2558 1145
Size: 1286 127 3029
Fragments arranged by size:
3029 1286 127
MspA1I CmG'CkG
Cuts at: 714 1061 1317 1325 1553 1867 2227 3240 3485
Size: 347 256 8 228 314 360 1013 245
Cuts at: 3485 714
Size: 1671
Fragments arranged by size:
1671 1013 360 347 314 256 245 228
8
NaeI GCC'GGC
Cuts at: 760 2623 760
Size: 1863 2579
NarI GG'CG_CC
Cuts at: 227 1289 1873 2121 227
Size: 1062 584 248 2548
Fragments arranged by size:
2548 1062 584 248
NcoI C'CATG_G
Cuts at: 1292 1292
Size: 4442
NgoMIV G'CCGG_C
Cuts at: 758 2621 758
Size: 1863 2579
NsiI A_TGCA'T
Cuts at: 950 950
Size: 4442
NspI r_CATG'y
Cuts at: 873 1495 1995 2902 873
Size: 622 500 907 2413
Fragments arranged by size:
2413 907 622 500
PasI CC'CwG_GG
Cuts at: 1011 1011
Size: 4442
PciI A'CATG_T
Cuts at: 2898 2898
Size: 4442
PmeI GTTT'AAAC
Cuts at: 1441 1441
Size: 4442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 718 750 3581 3613 718
Size: 32 2831 32 1547
Fragments arranged by size:
2831 1547 32 32
PpuMI rG'GwC_Cy
Cuts at: 1175 1175
Size: 4442
PsiI TTA'TAA
Cuts at: 185 185
Size: 4442
PstI C_TGCA'G
Cuts at: 1157 1489 2174 1157
Size: 332 685 3425
Fragments arranged by size:
3425 685 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4442
PvuII CAG'CTG
Cuts at: 714 1061 1867 2227 714
Size: 347 806 360 2929
Fragments arranged by size:
2929 806 360 347
RsrII CG'GwC_CG
Cuts at: 2637 2637
Size: 4442
SacI G_AGCT'C
Cuts at: 1456 1456
Size: 4442
SalI G'TCGA_C
Cuts at: 1479 1479
Size: 4442
SanDI GG'GwC_CC
Cuts at: 1175 1175
Size: 4442
SapI GCTCTTCn'nnn_
Cuts at: 2465 2675 2465
Size: 210 4232
SbfI CC_TGCA'GG
Cuts at: 1157 1489 1157
Size: 332 4110
ScaI AGT'ACT
Cuts at: 3959 3959
Size: 4442
SexAI A'CCwGG_T
Cuts at: 1433 1433
Size: 4442
SfcI C'TryA_G
Cuts at: 33 1153 1485 2170 3163 3354 33
Size: 1120 332 685 993 191 1121
Fragments arranged by size:
1121 1120 993 685 332 191
SfoI GGC'GCC
Cuts at: 228 1290 1874 2122 228
Size: 1062 584 248 2548
Fragments arranged by size:
2548 1062 584 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
SmaI CCC'GGG
Cuts at: 1464 1464
Size: 4442
SmlI C'TyrA_G
Cuts at: 1597 2512 3004 3266 1597
Size: 915 492 262 2773
Fragments arranged by size:
2773 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4442
SphI G_CATG'C
Cuts at: 873 1495 1995 873
Size: 622 500 3320
Fragments arranged by size:
3320 622 500
StyI C'CwwG_G
Cuts at: 293 1292 1576 1930 293
Size: 999 284 354 2805
Fragments arranged by size:
2805 999 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 567 1434 1444 2025 2158 2617 2826 567
Size: 867 10 581 133 459 209 2183
Fragments arranged by size:
2183 867 581 459 209 133 10
TatI w'GTAC_w
Cuts at: 842 3957 842
Size: 3115 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 4410
Tth111I GACn'n_nGTC
Cuts at: 2239 2239
Size: 4442
XbaI T'CTAG_A
Cuts at: 1473 1473
Size: 4442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1937 1937
Size: 4442
XmaI C'CCGG_G
Cuts at: 1462 1462
Size: 4442
XmnI GAAnn'nnTTC
Cuts at: 733 733
Size: 4442
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI ApaI AscI AvrII
BaeI BmgBI BmtI BsiWI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI