Restriction map for
pF1KB7747
(Circular) (Six-base) MAPSORT of: pF1KB7747.seq Check: 9583 from: 1 to: 4439
pF1KB7747 4439 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 2264 2696 3443 3711 463
Size: 1801 432 747 268 1191
Fragments arranged by size:
1801 1191 747 432 268
AflIII A'CryG_T
Cuts at: 1329 1343 1791 2895 3872 1329
Size: 14 448 1104 977 1896
Fragments arranged by size:
1896 1104 977 448 14
AgeI A'CCGG_T
Cuts at: 2827 2827
Size: 4439
AhdI GACnn_n'nnGTC
Cuts at: 1224 1224
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 379 1014 1744 3311 379
Size: 635 730 1567 1507
Fragments arranged by size:
1567 1507 730 635
ApaLI G'TGCA_C
Cuts at: 3209 3209
Size: 4439
ApoI r'AATT_y
Cuts at: 591 1443 3792 4048 591
Size: 852 2349 256 982
Fragments arranged by size:
2349 982 852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 1459 1459
Size: 4439
AvrII C'CTAG_G
Cuts at: 1060 1060
Size: 4439
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 1455 1869 2117 2152 1455
Size: 414 248 35 3742
Fragments arranged by size:
3742 414 248 35
BanII G_rGCy'C
Cuts at: 1453 2483 1453
Size: 1030 3409
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbsI GAAGACnn'nnnn_
Cuts at: 144 144
Size: 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 385 419 385
Size: 34 4405
BciVI GTATCCnnnnn_n'
Cuts at: 450 2337 3098 4439 450
Size: 1887 761 1341 450
Fragments arranged by size:
1887 1341 761 450
BclI T'GATC_A
Cuts at: 1076 1959 1076
Size: 883 3556
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4439
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 185 1551 185
Size: 1366 3073
Bme1580I G_kGCm'C
Cuts at: 2064 2157 3213 2064
Size: 93 1056 3290
Fragments arranged by size:
3290 1056 93
BmgBI CAC'GTC
Cuts at: 1330 1344 1330
Size: 14 4425
BmrI ACTGGGnnnn_n'
Cuts at: 543 1651 1732 2067 4211 543
Size: 1108 81 335 2144 771
Fragments arranged by size:
2144 1108 771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 691
Size: 4439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 259 683 1134 1615 2494 2986 3284 232
Size: 27 424 451 481 879 492 298 1387
Fragments arranged by size:
1387 879 492 481 451 424 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 760 4143 760
Size: 3383 1056
BsaAI yAC'GTr
Cuts at: 1792 1792
Size: 4439
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 1264 1870 2118 2820 4316 1264
Size: 606 248 702 1496 1387
Fragments arranged by size:
1496 1387 702 606 248
BsaWI w'CCGG_w
Cuts at: 613 1628 2149 2827 3101 3248 3659 613
Size: 1015 521 678 274 147 411 1393
Fragments arranged by size:
1393 1015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 844 874 844
Size: 30 4409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 411 544 411
Size: 133 4306
BseYI C'CCAG_C
Cuts at: 136 1864 2622 3199 3695 136
Size: 1728 758 577 496 880
Fragments arranged by size:
1728 880 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1174 1397 274
Size: 900 223 3316
Fragments arranged by size:
3316 900 223
BsiEI CG_ry'CG
Cuts at: 104 1981 2027 3235 104
Size: 1877 46 1208 1308
Fragments arranged by size:
1877 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1453 2231 2421 3213 1453
Size: 778 190 792 2679
Fragments arranged by size:
2679 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 493 493
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 1453 2064 2157 2231 2421 2483 3213 1453
Size: 611 93 74 190 62 730 2679
Fragments arranged by size:
2679 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3659 3659
Size: 4439
BspHI T'CATG_A
Cuts at: 3615 3615
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1489 1703 2005 2386 1489
Size: 214 302 381 3542
Fragments arranged by size:
3542 381 302 214
BsrBI CCG'CTC
Cuts at: 50 994 2731 2785 4269 4434 50
Size: 944 1737 54 1484 165 55
Fragments arranged by size:
1737 1484 944 165 55 54
BsrDI GCAATG_nn'
Cuts at: 351 2351 351
Size: 2000 2439
BsrFI r'CCGG_y
Cuts at: 125 2437 2618 2827 125
Size: 2312 181 209 1737
Fragments arranged by size:
2312 1737 209 181
BssSI C'ACGA_G
Cuts at: 884 2710 3068 884
Size: 1826 358 2255
Fragments arranged by size:
2255 1826 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstXI CCAn_nnnn'nTGG
Cuts at: 927 927
Size: 4439
BstYI r'GATC_y
Cuts at: 1 916 1109 1464 1931 1954 2289 2535 3547
Size: 915 193 355 467 23 335 246 1012
Cuts at: 3547 3633 3645 3991 1
Size: 86 12 346 449
Fragments arranged by size:
1012 915 467 449 355 346 335 246
193 86 23 12
BtgI C'CryG_G
Cuts at: 107 452 954 1245 107
Size: 345 502 291 3301
Fragments arranged by size:
3301 502 345 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 1682 1682
Size: 4439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1227 1262 1227
Size: 35 4404
DraI TTT'AAA
Cuts at: 1053 1438 1053
Size: 385 4054
DraIII CAC_nnn'GTG
Cuts at: 1375 1375
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3003 2145
Size: 858 3581
EaeI y'GGCC_r
Cuts at: 1248 2024 2198 2589 2616 3961 1248
Size: 776 174 391 27 1345 1726
Fragments arranged by size:
1726 1345 776 391 174 27
EagI C'GGCC_G
Cuts at: 2024 2024
Size: 4439
EarI CTCTTCn'nnn_
Cuts at: 211 763 2462 2672 211
Size: 552 1699 210 1978
Fragments arranged by size:
1978 1699 552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 2957 3103 4249 2647
Size: 310 146 1146 2837
Fragments arranged by size:
2837 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 691 2264 2696 3443 3711 463
Size: 228 1573 432 747 268 1191
Fragments arranged by size:
1573 1191 747 432 268 228
EcoO109I rG'GnC_Cy
Cuts at: 1057 1200 1578 1057
Size: 143 378 3918
Fragments arranged by size:
3918 378 143
EcoRI G'AATT_C
Cuts at: 591 1443 591
Size: 852 3587
EcoRV GAT'ATC
Cuts at: 504 504
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4439
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 237 1873 2121 3143 4122 237
Size: 1636 248 1022 979 554
Fragments arranged by size:
1636 1022 979 554 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 908 940 1083 1101 1115 1133 1210
Size: 32 347 32 143 18 14 18 77
Cuts at: 1210 1242 2281 2311 2313 2343 529
Size: 32 1039 30 2 30 2625
Fragments arranged by size:
2625 1039 347 143 77 32 32 32
30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 1478 1478
Size: 4439
HindIII A'AGCT_T
Cuts at: 910 910
Size: 4439
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 700 944 3110 3294 700
Size: 244 2166 184 1845
Fragments arranged by size:
2166 1845 244 184
MscI TGG'CCA
Cuts at: 2200 2200
Size: 4439
MslI CAynn'nnrTG
Cuts at: 344 1295 2428 2555 344
Size: 951 1133 127 2228
Fragments arranged by size:
2228 1133 951 127
MspA1I CmG'CkG
Cuts at: 140 376 640 1550 1864 2224 3237 3482 140
Size: 236 264 910 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 910 360 314 264 245 236
NaeI GCC'GGC
Cuts at: 127 2620 127
Size: 2493 1946
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NcoI C'CATG_G
Cuts at: 107 107
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 125 2618 125
Size: 2493 1946
NspI r_CATG'y
Cuts at: 560 1492 1992 2899 560
Size: 932 500 907 2100
Fragments arranged by size:
2100 932 907 500
PciI A'CATG_T
Cuts at: 2895 2895
Size: 4439
PfoI T'CCnGG_A
Cuts at: 728 1083 728
Size: 355 4084
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 1057 1057
Size: 4439
PstI C_TGCA'G
Cuts at: 1486 2171 1486
Size: 685 3754
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 376 640 1864 2224 376
Size: 264 1224 360 2591
Fragments arranged by size:
2591 1224 360 264
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 1482 2167 3160 3351 33
Size: 1449 685 993 191 1121
Fragments arranged by size:
1449 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 211 238 698 1149 1594 2509 3001 3263 211
Size: 27 460 451 445 915 492 262 1387
Fragments arranged by size:
1387 915 492 460 451 445 262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
StuI AGG'CCT
Cuts at: 1387 1387
Size: 4439
StyI C'CwwG_G
Cuts at: 107 292 317 1060 1573 1927 107
Size: 185 25 743 513 354 2619
Fragments arranged by size:
2619 743 513 354 185 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1387 2022 2155 2614 2823 1387
Size: 635 133 459 209 3003
Fragments arranged by size:
3003 635 459 209 133
TatI w'GTAC_w
Cuts at: 776 1380 3954 776
Size: 604 2574 1261
Fragments arranged by size:
2574 1261 604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4407
Tth111I GACn'n_nGTC
Cuts at: 148 2236 148
Size: 2088 2351
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1934 1934
Size: 4439
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
XmnI GAAnn'nnTTC
Cuts at: 1276 1276
Size: 4439
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI