Restriction map for
pF1KB7745
(Circular) (Six-base) MAPSORT of: pF1KB7745.seq Check: 1692 from: 1 to: 4424
pF1KB7745 4424 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 298 298
Size: 4424
AccI GT'mk_AC
Cuts at: 1007 1462 1007
Size: 455 3969
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 2249 2681 3428 3696 769
Size: 1480 432 747 268 1497
Fragments arranged by size:
1497 1480 747 432 268
AflIII A'CryG_T
Cuts at: 1776 2880 3857 1776
Size: 1104 977 2343
Fragments arranged by size:
2343 1104 977
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 4424
AlwNI CAG_nnn'CTG
Cuts at: 229 747 1275 1729 3296 229
Size: 518 528 454 1567 1357
Fragments arranged by size:
1567 1357 528 518 454
ApaI G_GGCC'C
Cuts at: 122 998 122
Size: 876 3548
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 1428 3777 4033 1428
Size: 2349 256 1819
Fragments arranged by size:
2349 1819 256
AseI AT'TA_AT
Cuts at: 20 1415 20
Size: 1395 3029
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 122 316 478 1444 122
Size: 194 162 966 3102
Fragments arranged by size:
3102 966 194 162
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 235 268 235
Size: 33 4391
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 162 264 279 286 1440 1854 2102 2137 162
Size: 102 15 7 1154 414 248 35 2449
Fragments arranged by size:
2449 1154 414 248 102 35 15 7
BanII G_rGCy'C
Cuts at: 122 178 998 1139 1438 2468 122
Size: 56 820 141 299 1030 2078
Fragments arranged by size:
2078 1030 820 299 141 56
BbeI G_GCGC'C
Cuts at: 268 283 1858 2106 268
Size: 15 1575 248 2586
Fragments arranged by size:
2586 1575 248 15
BbsI GAAGACnn'nnnn_
Cuts at: 493 710 493
Size: 217 4207
BbvCI CC'TCA_GC
Cuts at: 202 584 202
Size: 382 4042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 144 178 276 310 90
Size: 34 20 34 98 34 4204
Fragments arranged by size:
4204 98 34 34 34 20
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1307 1944 1307
Size: 637 3787
BglI GCCn_nnn'nGGC
Cuts at: 916 916
Size: 4424
BglII A'GATC_T
Cuts at: 1939 3976 1939
Size: 2037 2387
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 122 167 947 998 1018 2049 2142 3198 122
Size: 45 780 51 20 1031 93 1056 1348
Fragments arranged by size:
1348 1056 1031 780 93 51 45 20
BmgBI CAC'GTC
Cuts at: 597 597
Size: 4424
BmrI ACTGGGnnnn_n'
Cuts at: 585 1636 1717 2052 4196 585
Size: 1051 81 335 2144 813
Fragments arranged by size:
2144 1051 813 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 818 2791 818
Size: 1973 2451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1252 400
Size: 852 3572
Bpu10I CC'TnA_GC
Cuts at: 202 541 584 202
Size: 339 43 4042
Fragments arranged by size:
4042 339 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1600 2479 2971 3269 964
Size: 636 879 492 298 2119
Fragments arranged by size:
2119 879 636 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4128 4128
Size: 4424
BsaAI yAC'GTr
Cuts at: 1777 1777
Size: 4424
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 265 280 299 1855 2103 2805 4301 265
Size: 15 19 1556 248 702 1496 388
Fragments arranged by size:
1556 1496 702 388 248 19 15
BsaWI w'CCGG_w
Cuts at: 1613 2134 2812 3086 3233 3644 1613
Size: 521 678 274 147 411 2393
Fragments arranged by size:
2393 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 252 282 252
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1036 1066 1036
Size: 30 4394
BseYI C'CCAG_C
Cuts at: 142 212 222 454 507 735 1084 1100 1105
Size: 70 10 232 53 228 349 16 5
Cuts at: 1105 1175 1849 2607 3184 3680 142
Size: 70 674 758 577 496 886
Fragments arranged by size:
886 758 674 577 496 349 232 228
70 70 53 16 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1048 1314 619
Size: 429 266 3729
Fragments arranged by size:
3729 429 266
BsiEI CG_ry'CG
Cuts at: 104 140 1966 2012 3220 104
Size: 36 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46 36
BsiHKAI G_wGCw'C
Cuts at: 988 1438 2216 2406 3198 988
Size: 450 778 190 792 2214
Fragments arranged by size:
2214 792 778 450 190
Bsp1286I G_dGCh'C
Cuts at: 122 167 178 947 988 998 1018 1139 1438
Size: 45 11 769 41 10 20 121 299
Cuts at: 1438 2049 2142 2216 2406 2468 3198 122
Size: 611 93 74 190 62 730 1348
Fragments arranged by size:
1348 769 730 611 299 190 121 93
74 62 45 41 20 11 10
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 3600 3600
Size: 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 298 974 1474 1688 1990 2371 298
Size: 676 500 214 302 381 2351
Fragments arranged by size:
2351 676 500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2716 2770 4254 4419 50
Size: 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2336 2336
Size: 4424
BsrFI r'CCGG_y
Cuts at: 342 350 357 2422 2603 2812 342
Size: 8 7 2065 181 209 1954
Fragments arranged by size:
2065 1954 209 181 8 7
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 1801 1801
Size: 4424
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 633 633
Size: 4424
BstYI r'GATC_y
Cuts at: 1 375 1449 1916 1939 2274 2520 3532 3618
Size: 374 1074 467 23 335 246 1012 86
Cuts at: 3618 3630 3976 1
Size: 12 346 449
Fragments arranged by size:
1074 1012 467 449 374 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 309 309
Size: 4424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 184 348 2374 2555 2718 184
Size: 164 2026 181 163 1890
Fragments arranged by size:
2026 1890 181 164 163
BtsI GCAGTG_nn'
Cuts at: 1667 1667
Size: 4424
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 2130 2988 2130
Size: 858 3566
EaeI y'GGCC_r
Cuts at: 137 190 359 2009 2183 2574 2601 3946 137
Size: 53 169 1650 174 391 27 1345 615
Fragments arranged by size:
1650 1345 615 391 174 169 53 27
EagI C'GGCC_G
Cuts at: 137 2009 137
Size: 1872 2552
EarI CTCTTCn'nnn_
Cuts at: 1387 2447 2657 1387
Size: 1060 210 3154
Fragments arranged by size:
3154 1060 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 2942 3088 4234 2632
Size: 310 146 1146 2822
Fragments arranged by size:
2822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 769 1252 2249 2681 3428 3696 400
Size: 369 483 997 432 747 268 1128
Fragments arranged by size:
1128 997 747 483 432 369 268
EcoNI CCTnn'n_nnAGG
Cuts at: 823 823
Size: 4424
EcoO109I rG'GnC_Cy
Cuts at: 118 119 561 918 994 1182 1404 1563 118
Size: 1 442 357 76 188 222 159 2979
Fragments arranged by size:
2979 442 357 222 188 159 76 1
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
EcoRV GAT'ATC
Cuts at: 870 870
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 268 283 1168 1858 2106 3128 4107 268
Size: 15 885 690 248 1022 979 585
Fragments arranged by size:
1022 979 885 690 585 248 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 2266 2296 2298 2328 252
Size: 32 1982 30 2 30 2348
Fragments arranged by size:
2348 1982 32 30 30 2
HincII GTy'rAC
Cuts at: 1463 1463
Size: 4424
KasI G'GCGC_C
Cuts at: 264 279 1854 2102 264
Size: 15 1575 248 2586
Fragments arranged by size:
2586 1575 248 15
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3095 3279 3095
Size: 184 4240
MscI TGG'CCA
Cuts at: 192 2185 192
Size: 1993 2431
MslI CAynn'nnrTG
Cuts at: 439 628 2413 2540 439
Size: 189 1785 127 2323
Fragments arranged by size:
2323 1785 189 127
MspA1I CmG'CkG
Cuts at: 142 249 277 583 735 1535 1849 2209 3222
Size: 107 28 306 152 800 314 360 1013
Cuts at: 3222 3467 142
Size: 245 1099
Fragments arranged by size:
1099 1013 800 360 314 306 245 152
107 28
NaeI GCC'GGC
Cuts at: 344 352 359 2605 344
Size: 8 7 2246 2163
Fragments arranged by size:
2246 2163 8 7
NarI GG'CG_CC
Cuts at: 265 280 1855 2103 265
Size: 15 1575 248 2586
Fragments arranged by size:
2586 1575 248 15
NgoMIV G'CCGG_C
Cuts at: 342 350 357 2603 342
Size: 8 7 2246 2163
Fragments arranged by size:
2246 2163 8 7
NspI r_CATG'y
Cuts at: 1477 1977 2884 1477
Size: 500 907 3017
Fragments arranged by size:
3017 907 500
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PfoI T'CCnGG_A
Cuts at: 563 563
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1255 1287 3563 3595 1255
Size: 32 2276 32 2084
Fragments arranged by size:
2276 2084 32 32
PpuMI rG'GwC_Cy
Cuts at: 561 1404 561
Size: 843 3581
PspOMI G'GGCC_C
Cuts at: 118 994 118
Size: 876 3548
PstI C_TGCA'G
Cuts at: 971 1256 1471 2156 971
Size: 285 215 685 3239
Fragments arranged by size:
3239 685 285 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 735 1849 2209 735
Size: 1114 360 2950
Fragments arranged by size:
2950 1114 360
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 967 1252 1467 2152 3145 3336 33
Size: 934 285 215 685 993 191 1121
Fragments arranged by size:
1121 993 934 685 285 215 191
SfoI GGC'GCC
Cuts at: 266 281 1856 2104 266
Size: 15 1575 248 2586
Fragments arranged by size:
2586 1575 248 15
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 124 480 1446 124
Size: 356 966 3102
Fragments arranged by size:
3102 966 356
SmlI C'TyrA_G
Cuts at: 979 1579 2494 2986 3248 979
Size: 600 915 492 262 2155
Fragments arranged by size:
2155 915 600 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
StuI AGG'CCT
Cuts at: 775 775
Size: 4424
StyI C'CwwG_G
Cuts at: 128 422 776 990 1208 1325 1398 1558 1912
Size: 294 354 214 218 117 73 160 354
Cuts at: 1912 128
Size: 2640
Fragments arranged by size:
2640 354 354 294 218 214 160 117
73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1003 1259 2007 2140 2599 2808 1003
Size: 256 748 133 459 209 2619
Fragments arranged by size:
2619 748 459 256 209 133
TatI w'GTAC_w
Cuts at: 3939 3939
Size: 4424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4392
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 843 1919 369
Size: 474 1076 2874
Fragments arranged by size:
2874 1076 474
XmaI C'CCGG_G
Cuts at: 122 478 1444 122
Size: 356 966 3102
Fragments arranged by size:
3102 966 356
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI