Restriction map for
pF1KB7744
(Circular) (Six-base) MAPSORT of: pF1KB7744.seq Check: 1042 from: 1 to: 4574
pF1KB7744 4574 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 241 1373 241
Size: 1132 3442
AccI GT'mk_AC
Cuts at: 1612 1612
Size: 4574
Acc65I G'GTAC_C
Cuts at: 1590 1590
Size: 4574
AclI AA'CG_TT
Cuts at: 4414 4414
Size: 4574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 499 2399 2831 3578 3846 370
Size: 129 1900 432 747 268 1098
Fragments arranged by size:
1900 1098 747 432 268 129
AfeI AGC'GCT
Cuts at: 1333 1333
Size: 4574
AflIII A'CryG_T
Cuts at: 1926 3030 4007 1926
Size: 1104 977 2493
Fragments arranged by size:
2493 1104 977
AgeI A'CCGG_T
Cuts at: 2962 2962
Size: 4574
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 974 1006 974
Size: 32 4542
AlwNI CAG_nnn'CTG
Cuts at: 267 330 420 1879 3446 267
Size: 63 90 1459 1567 1395
Fragments arranged by size:
1567 1459 1395 90 63
ApaI G_GGCC'C
Cuts at: 1081 1081
Size: 4574
ApaLI G'TGCA_C
Cuts at: 3344 3344
Size: 4574
ApoI r'AATT_y
Cuts at: 1578 3927 4183 1578
Size: 2349 256 1969
Fragments arranged by size:
2349 1969 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4574
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
AvaI C'yCGr_G
Cuts at: 443 631 1337 1515 1594 443
Size: 188 706 178 79 3423
Fragments arranged by size:
3423 706 188 178 79
AvrII C'CTAG_G
Cuts at: 841 841
Size: 4574
BamHI G'GATC_C
Cuts at: 989 1599 989
Size: 610 3964
BanI G'GyrC_C
Cuts at: 1590 2004 2252 2287 1590
Size: 414 248 35 3877
Fragments arranged by size:
3877 414 248 35
BanII G_rGCy'C
Cuts at: 291 444 772 1022 1081 1588 2618 291
Size: 153 328 250 59 507 1030 2247
Fragments arranged by size:
2247 1030 507 328 250 153 59
BbeI G_GCGC'C
Cuts at: 2008 2256 2008
Size: 248 4326
BbvCI CC'TCA_GC
Cuts at: 857 1328 857
Size: 471 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2472 3233 4574 2472
Size: 761 1341 2472
Fragments arranged by size:
2472 1341 761
BclI T'GATC_A
Cuts at: 203 1116 2094 203
Size: 913 978 2683
Fragments arranged by size:
2683 978 913
BglI GCCn_nnn'nGGC
Cuts at: 143 278 1527 143
Size: 135 1249 3190
Fragments arranged by size:
3190 1249 135
BglII A'GATC_T
Cuts at: 2089 4126 2089
Size: 2037 2537
BlpI GC'TnA_GC
Cuts at: 659 1090 1686 659
Size: 431 596 3547
Fragments arranged by size:
3547 596 431
Bme1580I G_kGCm'C
Cuts at: 1081 1127 2199 2292 3348 1081
Size: 46 1072 93 1056 2307
Fragments arranged by size:
2307 1072 1056 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 541 1786 1867 2202 4346 541
Size: 1245 81 335 2144 769
Fragments arranged by size:
2144 1245 769 335 81
BmtI G_CTAG'C
Cuts at: 169 250 169
Size: 81 4493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 525 1541 2941 525
Size: 1016 1400 2158
Fragments arranged by size:
2158 1400 1016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 456 563 977 1032 1044 389
Size: 67 107 414 55 12 3919
Fragments arranged by size:
3919 414 107 67 55 12
Bpu10I CC'TnA_GC
Cuts at: 376 857 1328 376
Size: 481 471 3622
Fragments arranged by size:
3622 481 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 1243 1750 2629 3121 3419 495
Size: 748 507 879 492 298 1650
Fragments arranged by size:
1650 879 748 507 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1220 4278 1220
Size: 3058 1516
BsaAI yAC'GTr
Cuts at: 746 1927 746
Size: 1181 3393
BsaBI GATnn'nnATC
Cuts at: 6 2088 4125 6
Size: 2082 2037 455
Fragments arranged by size:
2082 2037 455
BsaHI Gr'CG_yC
Cuts at: 2005 2253 2955 4451 2005
Size: 248 702 1496 2128
Fragments arranged by size:
2128 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1763 2284 2962 3236 3383 3794 1763
Size: 521 678 274 147 411 2543
Fragments arranged by size:
2543 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1381 1411 1381
Size: 30 4544
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 1035 1500 1503 508
Size: 527 465 3 3579
Fragments arranged by size:
3579 527 465 3
BseYI C'CCAG_C
Cuts at: 133 267 323 810 1420 1462 1999 2757 3334
Size: 134 56 487 610 42 537 758 577
Cuts at: 3334 3830 133
Size: 496 877
Fragments arranged by size:
877 758 610 577 537 496 487 134
56 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 433
Size: 4574
BsiEI CG_ry'CG
Cuts at: 104 2116 2162 3370 104
Size: 2012 46 1208 1308
Fragments arranged by size:
2012 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 743 1234 1588 2366 2556 3348 743
Size: 491 354 778 190 792 1969
Fragments arranged by size:
1969 792 778 491 354 190
BsmI GAATG_Cn'
Cuts at: 926 926
Size: 4574
BsmBI CGTCTCn'nnnn_
Cuts at: 1267 1529 1267
Size: 262 4312
Bsp1286I G_dGCh'C
Cuts at: 291 444 743 772 1022 1081 1127 1234 1588
Size: 153 299 29 250 59 46 107 354
Cuts at: 1588 2199 2292 2366 2556 2618 3348 291
Size: 611 93 74 190 62 730 1517
Fragments arranged by size:
1517 730 611 354 299 250 190 153
107 93 74 62 59 46 29
BspEI T'CCGG_A
Cuts at: 3794 3794
Size: 4574
BspHI T'CATG_A
Cuts at: 1155 3750 1155
Size: 2595 1979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 241 1373 1624 1838 2140 2521 241
Size: 1132 251 214 302 381 2294
Fragments arranged by size:
2294 1132 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 1384 1493 2866 2920 4404 4569 50
Size: 1334 109 1373 54 1484 165 55
Fragments arranged by size:
1484 1373 1334 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 2486 2486
Size: 4574
BsrFI r'CCGG_y
Cuts at: 1411 2572 2753 2962 1411
Size: 1161 181 209 3023
Fragments arranged by size:
3023 1161 209 181
BssSI C'ACGA_G
Cuts at: 849 2845 3203 849
Size: 1996 358 2220
Fragments arranged by size:
2220 1996 358
BstAPI GCAn_nnn'nTGC
Cuts at: 738 1147 1951 738
Size: 409 804 3361
Fragments arranged by size:
3361 804 409
BstBI TT'CG_AA
Cuts at: 2935 2935
Size: 4574
BstXI CCAn_nnnn'nTGG
Cuts at: 405 405
Size: 4574
BstYI r'GATC_y
Cuts at: 1 468 989 1599 2066 2089 2424 2670 3682
Size: 467 521 610 467 23 335 246 1012
Cuts at: 3682 3768 3780 4126 1
Size: 86 12 346 449
Fragments arranged by size:
1012 610 521 467 467 449 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2524 2705 2868 2524
Size: 181 163 4230
Fragments arranged by size:
4230 181 163
BtsI GCAGTG_nn'
Cuts at: 1817 1817
Size: 4574
DraI TTT'AAA
Cuts at: 1573 1573
Size: 4574
DraIII CAC_nnn'GTG
Cuts at: 193 193
Size: 4574
DrdI GACnn_nn'nnGTC
Cuts at: 2280 3138 2280
Size: 858 3716
EaeI y'GGCC_r
Cuts at: 181 395 708 1349 2159 2333 2724 2751 4096
Size: 214 313 641 810 174 391 27 1345
Cuts at: 4096 181
Size: 659
Fragments arranged by size:
1345 810 659 641 391 313 214 174
27
EagI C'GGCC_G
Cuts at: 2159 2159
Size: 4574
EarI CTCTTCn'nnn_
Cuts at: 419 1103 2597 2807 419
Size: 684 1494 210 2186
Fragments arranged by size:
2186 1494 684 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2782 3092 3238 4384 2782
Size: 310 146 1146 2972
Fragments arranged by size:
2972 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1586 1586
Size: 4574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 389 456 499 563 977 1032 1044 2399
Size: 19 67 43 64 414 55 12 1355
Cuts at: 2399 2831 3578 3846 370
Size: 432 747 268 1098
Fragments arranged by size:
1355 1098 747 432 414 268 67 64
55 43 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 388 424 1058 1072 1515 388
Size: 36 634 14 443 3447
Fragments arranged by size:
3447 634 443 36 14
EcoO109I rG'GnC_Cy
Cuts at: 380 401 537 845 861 1077 1078 1713 380
Size: 21 136 308 16 216 1 635 3241
Fragments arranged by size:
3241 635 308 216 136 21 16 1
EcoRI G'AATT_C
Cuts at: 1578 1578
Size: 4574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4574
FspI TGC'GCA
Cuts at: 787 2355 787
Size: 1568 3006
HaeII r_GCGC'y
Cuts at: 1335 2008 2256 3278 4257 1335
Size: 673 248 1022 979 1652
Fragments arranged by size:
1652 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 287 319 744 776 1472 1504 2416 2446 2448
Size: 32 425 32 696 32 912 30 2
Cuts at: 2448 2478 287
Size: 30 2383
Fragments arranged by size:
2383 912 696 425 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1613 1613
Size: 4574
KasI G'GCGC_C
Cuts at: 2004 2252 2004
Size: 248 4326
KpnI G_GTAC'C
Cuts at: 1594 1594
Size: 4574
MluI A'CGCG_T
Cuts at: 4007 4007
Size: 4574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3245 3429 3245
Size: 184 4390
MscI TGG'CCA
Cuts at: 710 2335 710
Size: 1625 2949
MslI CAynn'nnrTG
Cuts at: 2563 2690 2563
Size: 127 4447
MspA1I CmG'CkG
Cuts at: 267 1394 1685 1999 2359 3372 3617 267
Size: 1127 291 314 360 1013 245 1224
Fragments arranged by size:
1224 1127 1013 360 314 291 245
NaeI GCC'GGC
Cuts at: 1413 2755 1413
Size: 1342 3232
NarI GG'CG_CC
Cuts at: 2005 2253 2005
Size: 248 4326
NgoMIV G'CCGG_C
Cuts at: 1411 2753 1411
Size: 1342 3232
NheI G'CTAG_C
Cuts at: 165 246 165
Size: 81 4493
NspI r_CATG'y
Cuts at: 434 1449 1627 2127 3034 434
Size: 1015 178 500 907 1974
Fragments arranged by size:
1974 1015 907 500 178
PasI CC'CwG_GG
Cuts at: 1074 1096 1074
Size: 22 4552
PciI A'CATG_T
Cuts at: 3030 3030
Size: 4574
PflMI CCAn_nnn'nTGG
Cuts at: 240 267 240
Size: 27 4547
PmeI GTTT'AAAC
Cuts at: 1573 1573
Size: 4574
PmlI CAC'GTG
Cuts at: 746 746
Size: 4574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4542
PpuMI rG'GwC_Cy
Cuts at: 537 537
Size: 4574
PspOMI G'GGCC_C
Cuts at: 1077 1077
Size: 4574
PspXI vC'TCGA_Gb
Cuts at: 443 1515 443
Size: 1072 3502
PstI C_TGCA'G
Cuts at: 365 1621 2306 365
Size: 1256 685 2633
Fragments arranged by size:
2633 1256 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4574
PvuII CAG'CTG
Cuts at: 267 1999 2359 267
Size: 1732 360 2482
Fragments arranged by size:
2482 1732 360
RsrII CG'GwC_CG
Cuts at: 2769 2769
Size: 4574
SacI G_AGCT'C
Cuts at: 1588 1588
Size: 4574
SalI G'TCGA_C
Cuts at: 1611 1611
Size: 4574
SanDI GG'GwC_CC
Cuts at: 537 537
Size: 4574
SapI GCTCTTCn'nnn_
Cuts at: 2597 2807 2597
Size: 210 4364
SbfI CC_TGCA'GG
Cuts at: 1621 1621
Size: 4574
ScaI AGT'ACT
Cuts at: 4091 4091
Size: 4574
SexAI A'CCwGG_T
Cuts at: 673 673
Size: 4574
SfcI C'TryA_G
Cuts at: 33 361 1314 1617 2302 3295 3486 33
Size: 328 953 303 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 328 303 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 278 1527 278
Size: 1249 3325
SfoI GGC'GCC
Cuts at: 2006 2254 2006
Size: 248 4326
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
SmaI CCC'GGG
Cuts at: 1339 1596 1339
Size: 257 4317
SmlI C'TyrA_G
Cuts at: 443 510 1258 1515 1729 2644 3136 3398 443
Size: 67 748 257 214 915 492 262 1619
Fragments arranged by size:
1619 915 748 492 262 257 214 67
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4574
SphI G_CATG'C
Cuts at: 434 1627 2127 434
Size: 1193 500 2881
Fragments arranged by size:
2881 1193 500
StuI AGG'CCT
Cuts at: 476 476
Size: 4574
StyI C'CwwG_G
Cuts at: 404 841 865 1708 2062 404
Size: 437 24 843 354 2916
Fragments arranged by size:
2916 843 437 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1202 2157 2290 2749 2958 1202
Size: 955 133 459 209 2818
Fragments arranged by size:
2818 955 459 209 133
TatI w'GTAC_w
Cuts at: 4089 4089
Size: 4574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4091 4123 4091
Size: 32 4542
Tth111I GACn'n_nGTC
Cuts at: 675 2371 675
Size: 1696 2878
XbaI T'CTAG_A
Cuts at: 571 1605 571
Size: 1034 3540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 703 2069 703
Size: 1366 3208
XhoI C'TCGA_G
Cuts at: 443 1515 443
Size: 1072 3502
XmaI C'CCGG_G
Cuts at: 1337 1594 1337
Size: 257 4317
XmnI GAAnn'nnTTC
Cuts at: 1306 1306
Size: 4574
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI BaeI BbsI
BcgI BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PfoI PshAI PsiI PsrI SacII SgrAI SnaBI SrfI
SspI SwaI ZraI