Restriction map for
pF1KB7741
(Circular) (Six-base) MAPSORT of: pF1KB7741.seq Check: 8484 from: 1 to: 4421
pF1KB7741 4421 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 2246 2678 3425 3693 201
Size: 2045 432 747 268 929
Fragments arranged by size:
2045 929 747 432 268
AflIII A'CryG_T
Cuts at: 1773 2877 3854 1773
Size: 1104 977 2340
Fragments arranged by size:
2340 1104 977
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 1726 3293 1726
Size: 1567 2854
ApaLI G'TGCA_C
Cuts at: 575 3191 575
Size: 2616 1805
ApoI r'AATT_y
Cuts at: 830 1425 3774 4030 830
Size: 595 2349 256 1221
Fragments arranged by size:
2349 1221 595 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 1441 1441
Size: 4421
BamHI G'GATC_C
Cuts at: 1446 1446
Size: 4421
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 477 1101 1408 1435 2465 477
Size: 624 307 27 1030 2433
Fragments arranged by size:
2433 1030 624 307 27
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 429 1176 1260 429
Size: 747 84 3590
Fragments arranged by size:
3590 747 84
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BfrBI ATG'CAT
Cuts at: 762 762
Size: 4421
BglII A'GATC_T
Cuts at: 928 1936 3973 928
Size: 1008 2037 1376
Fragments arranged by size:
2037 1376 1008
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 579 2046 2139 3195 579
Size: 1467 93 1056 1805
Fragments arranged by size:
1805 1467 1056 93
BmgBI CAC'GTC
Cuts at: 1076 1076
Size: 4421
BmrI ACTGGGnnnn_n'
Cuts at: 560 812 1151 1633 1714 2049 4193 560
Size: 252 339 482 81 335 2144 788
Fragments arranged by size:
2144 788 482 339 335 252 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1048 2788 1048
Size: 1740 2681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 788 872 961 1127 1295 419
Size: 369 84 89 166 168 3545
Fragments arranged by size:
3545 369 168 166 89 84
Bpu10I CC'TnA_GC
Cuts at: 1402 1402
Size: 4421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 600 4125 600
Size: 3525 896
BsaAI yAC'GTr
Cuts at: 1774 1774
Size: 4421
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 523 1852 2100 2802 4298 523
Size: 1329 248 702 1496 646
Fragments arranged by size:
1496 1329 702 646 248
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3083 3230 3641 1610
Size: 521 678 274 147 411 2390
Fragments arranged by size:
2390 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4421
BseYI C'CCAG_C
Cuts at: 1846 2604 3181 3677 1846
Size: 758 577 496 2590
Fragments arranged by size:
2590 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1260 1260
Size: 4421
BsiEI CG_ry'CG
Cuts at: 104 236 1963 2009 3217 104
Size: 132 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 132 46
BsiHKAI G_wGCw'C
Cuts at: 477 579 1101 1435 2213 2403 3195 477
Size: 102 522 334 778 190 792 1703
Fragments arranged by size:
1703 792 778 522 334 190 102
BsmI GAATG_Cn'
Cuts at: 622 622
Size: 4421
BsmBI CGTCTCn'nnnn_
Cuts at: 514 514
Size: 4421
Bsp1286I G_dGCh'C
Cuts at: 477 579 1101 1408 1435 2046 2139 2213 2403
Size: 102 522 307 27 611 93 74 190
Cuts at: 2403 2465 3195 477
Size: 62 730 1703
Fragments arranged by size:
1703 730 611 522 307 190 102 93
74 62 27
BspEI T'CCGG_A
Cuts at: 3641 3641
Size: 4421
BspHI T'CATG_A
Cuts at: 1341 3597 1341
Size: 2256 2165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1471 1685 1987 2368 892
Size: 579 214 302 381 2945
Fragments arranged by size:
2945 579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2333 2333
Size: 4421
BsrFI r'CCGG_y
Cuts at: 2419 2600 2809 2419
Size: 181 209 4031
Fragments arranged by size:
4031 209 181
BsrGI T'GTAC_A
Cuts at: 1210 1210
Size: 4421
BssSI C'ACGA_G
Cuts at: 2692 3050 2692
Size: 358 4063
BstAPI GCAn_nnn'nTGC
Cuts at: 626 1798 626
Size: 1172 3249
BstBI TT'CG_AA
Cuts at: 744 1167 2782 744
Size: 423 1615 2383
Fragments arranged by size:
2383 1615 423
BstEII G'GTnAC_C
Cuts at: 904 904
Size: 4421
BstXI CCAn_nnnn'nTGG
Cuts at: 1378 1378
Size: 4421
BstYI r'GATC_y
Cuts at: 1 928 1446 1913 1936 2271 2517 3529 3615
Size: 927 518 467 23 335 246 1012 86
Cuts at: 3615 3627 3973 1
Size: 12 346 449
Fragments arranged by size:
1012 927 518 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1664
Size: 4421
ClaI AT'CG_AT
Cuts at: 809 809
Size: 4421
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 1358 2006 2180 2571 2598 3943 1358
Size: 648 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 648 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 2939 3085 4231 2629
Size: 310 146 1146 2819
Fragments arranged by size:
2819 1146 310 146
EcoICRI GAG'CTC
Cuts at: 475 1099 1433 475
Size: 624 334 3463
Fragments arranged by size:
3463 624 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 419 788 872 961 1127 1295 2246 2678
Size: 218 369 84 89 166 168 951 432
Cuts at: 2678 3425 3693 201
Size: 747 268 929
Fragments arranged by size:
951 929 747 432 369 268 218 168
166 89 84
EcoO109I rG'GnC_Cy
Cuts at: 692 1560 692
Size: 868 3553
EcoRI G'AATT_C
Cuts at: 1425 1425
Size: 4421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2263 2293 2295 2325 2263
Size: 30 2 30 4359
Fragments arranged by size:
4359 30 30 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 216 3092 3276 216
Size: 2876 184 1361
Fragments arranged by size:
2876 1361 184
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 414 1004 1206 2410 2537 414
Size: 590 202 1204 127 2298
Fragments arranged by size:
2298 1204 590 202 127
MspA1I CmG'CkG
Cuts at: 1532 1846 2206 3219 3464 1532
Size: 314 360 1013 245 2489
Fragments arranged by size:
2489 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 107 107
Size: 4421
NdeI CA'TA_TG
Cuts at: 1203 1203
Size: 4421
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NsiI A_TGCA'T
Cuts at: 764 764
Size: 4421
NspI r_CATG'y
Cuts at: 1474 1974 2881 1474
Size: 500 907 3014
Fragments arranged by size:
3014 907 500
PciI A'CATG_T
Cuts at: 2877 2877
Size: 4421
PflMI CCAn_nnn'nTGG
Cuts at: 282 282
Size: 4421
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 4421
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 3560 3592 214
Size: 32 3314 32 1043
Fragments arranged by size:
3314 1043 32 32
PpuMI rG'GwC_Cy
Cuts at: 692 692
Size: 4421
PstI C_TGCA'G
Cuts at: 1468 2153 1468
Size: 685 3736
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1846 2206 1846
Size: 360 4061
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 477 1101 1435 477
Size: 624 334 3463
Fragments arranged by size:
3463 624 334
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 192 1464 2149 3142 3333 33
Size: 159 1272 685 993 191 1121
Fragments arranged by size:
1272 1121 993 685 191 159
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 1576 2491 2983 3245 1576
Size: 915 492 262 2752
Fragments arranged by size:
2752 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
StyI C'CwwG_G
Cuts at: 107 1555 1909 107
Size: 1448 354 2619
Fragments arranged by size:
2619 1448 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 222 1223 2004 2137 2596 2805 163
Size: 59 1001 781 133 459 209 1779
Fragments arranged by size:
1779 1001 781 459 209 133 59
TatI w'GTAC_w
Cuts at: 1210 3936 1210
Size: 2726 1695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1916 114
Size: 1802 2619
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 436 436
Size: 4421
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
BsaXI BsiWI BssHII BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI