Restriction map for
pF1KB7731
(Circular) (Six-base) MAPSORT of: pF1KB7731.seq Check: 5287 from: 1 to: 4409
pF1KB7731 4409 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4409
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
AfeI AGC'GCT
Cuts at: 1090 1090
Size: 4409
AflIII A'CryG_T
Cuts at: 1761 2865 3842 1761
Size: 1104 977 2328
Fragments arranged by size:
2328 1104 977
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 182 394 1090 182
Size: 212 696 3501
Fragments arranged by size:
3501 696 212
AlwNI CAG_nnn'CTG
Cuts at: 135 304 1070 1714 3281 135
Size: 169 766 644 1567 1263
Fragments arranged by size:
1567 1263 766 644 169
ApaI G_GGCC'C
Cuts at: 548 548
Size: 4409
ApaLI G'TGCA_C
Cuts at: 150 3179 150
Size: 3029 1380
ApoI r'AATT_y
Cuts at: 742 1413 3762 4018 742
Size: 671 2349 256 1133
Fragments arranged by size:
2349 1133 671 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 931 1056 1206 1425 1839 2087 2122 931
Size: 125 150 219 414 248 35 3218
Fragments arranged by size:
3218 414 248 219 150 125 35
BanII G_rGCy'C
Cuts at: 325 342 548 673 1115 1423 2453 325
Size: 17 206 125 442 308 1030 2281
Fragments arranged by size:
2281 1030 442 308 206 125 17
BbeI G_GCGC'C
Cuts at: 935 1060 1210 1843 2091 935
Size: 125 150 633 248 3253
Fragments arranged by size:
3253 633 248 150 125
BbsI GAAGACnn'nnnn_
Cuts at: 1218 1218
Size: 4409
BbvCI CC'TCA_GC
Cuts at: 564 564
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1176 1210 1176
Size: 34 4375
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 677 1054 677
Size: 377 4032
BglII A'GATC_T
Cuts at: 584 824 1341 1924 3961 584
Size: 240 517 583 2037 1032
Fragments arranged by size:
2037 1032 583 517 240
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 154 391 548 977 2034 2127 3183 154
Size: 237 157 429 1057 93 1056 1380
Fragments arranged by size:
1380 1057 1056 429 237 157 93
BmrI ACTGGGnnnn_n'
Cuts at: 401 1621 1702 2037 4181 401
Size: 1220 81 335 2144 629
Fragments arranged by size:
2144 1220 629 335 81
BmtI G_CTAG'C
Cuts at: 899 899
Size: 4409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 379 2776 379
Size: 2397 2012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 530 157
Size: 373 4036
Bpu10I CC'TnA_GC
Cuts at: 564 908 564
Size: 344 4065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2956 3254 1585
Size: 879 492 298 2740
Fragments arranged by size:
2740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4113 4113
Size: 4409
BsaAI yAC'GTr
Cuts at: 945 1179 1762 945
Size: 234 583 3592
Fragments arranged by size:
3592 583 234
BsaBI GATnn'nnATC
Cuts at: 6 823 1923 3960 6
Size: 817 1100 2037 455
Fragments arranged by size:
2037 1100 817 455
BsaHI Gr'CG_yC
Cuts at: 932 1057 1207 1840 2088 2790 4286 932
Size: 125 150 633 248 702 1496 1055
Fragments arranged by size:
1496 1055 702 633 248 150 125
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 503 533 503
Size: 30 4379
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 458 552 458
Size: 94 4315
BseYI C'CCAG_C
Cuts at: 342 681 1834 2592 3169 3665 342
Size: 339 1153 758 577 496 1086
Fragments arranged by size:
1153 1086 758 577 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 385 772 1951 1997 3205 104
Size: 281 387 1179 46 1208 1308
Fragments arranged by size:
1308 1208 1179 387 281 46
BsiHKAI G_wGCw'C
Cuts at: 154 325 1423 2201 2391 3183 154
Size: 171 1098 778 190 792 1380
Fragments arranged by size:
1380 1098 792 778 190 171
BsmBI CGTCTCn'nnnn_
Cuts at: 1367 1367
Size: 4409
Bsp1286I G_dGCh'C
Cuts at: 154 325 342 391 548 673 977 1115 1423
Size: 171 17 49 157 125 304 138 308
Cuts at: 1423 2034 2127 2201 2391 2453 3183 154
Size: 611 93 74 190 62 730 1380
Fragments arranged by size:
1380 730 611 308 304 190 171 157
138 125 93 74 62 49 17
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 803 1166 1459 1673 1975 2356 803
Size: 363 293 214 302 381 2856
Fragments arranged by size:
2856 381 363 302 293 214
BsrBI CCG'CTC
Cuts at: 50 924 2701 2755 4239 4404 50
Size: 874 1777 54 1484 165 55
Fragments arranged by size:
1777 1484 874 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 767 839 891 2407 2588 2797 767
Size: 72 52 1516 181 209 2379
Fragments arranged by size:
2379 1516 209 181 72 52
BssHII G'CGCG_C
Cuts at: 716 716
Size: 4409
BssSI C'ACGA_G
Cuts at: 711 2680 3038 711
Size: 1969 358 2082
Fragments arranged by size:
2082 1969 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 282 430 1309 282
Size: 148 879 3382
Fragments arranged by size:
3382 879 148
BstYI r'GATC_y
Cuts at: 1 584 824 1341 1434 1901 1924 2259 2505
Size: 583 240 517 93 467 23 335 246
Cuts at: 2505 3517 3603 3615 3961 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 583 517 467 449 346 335 246
240 93 86 23 12
BtgI C'CryG_G
Cuts at: 1121 1202 1308 1121
Size: 81 106 4222
Fragments arranged by size:
4222 106 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 641 1652 641
Size: 1011 3398
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 382 769 788 939 957 1043 1259 1994 2168
Size: 387 19 151 18 86 216 735 174
Cuts at: 2168 2559 2586 3931 382
Size: 391 27 1345 860
Fragments arranged by size:
1345 860 735 391 387 216 174 151
86 27 19 18
EagI C'GGCC_G
Cuts at: 382 769 1994 382
Size: 387 1225 2797
Fragments arranged by size:
2797 1225 387
EarI CTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2927 3073 4219 2617
Size: 310 146 1146 2807
Fragments arranged by size:
2807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 323 1421 323
Size: 1098 3311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 530 2234 2666 3413 3681 157
Size: 373 1704 432 747 268 885
Fragments arranged by size:
1704 885 747 432 373 268
EcoO109I rG'GnC_Cy
Cuts at: 132 1548 132
Size: 1416 2993
EcoRI G'AATT_C
Cuts at: 742 1413 742
Size: 671 3738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2428 603
Size: 1825 2584
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 935 1060 1092 1102 1154 1210 1843 2091 3113
Size: 125 32 10 52 56 633 248 1022
Cuts at: 3113 4092 935
Size: 979 1252
Fragments arranged by size:
1252 1022 979 633 248 125 56 52
32 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 2251 2281 2283 2313 982
Size: 32 1237 30 2 30 3078
Fragments arranged by size:
3078 1237 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 931 1056 1206 1839 2087 931
Size: 125 150 633 248 3253
Fragments arranged by size:
3253 633 248 150 125
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3264 3080
Size: 184 4225
MscI TGG'CCA
Cuts at: 790 941 2170 790
Size: 151 1229 3029
Fragments arranged by size:
3029 1229 151
MslI CAynn'nnrTG
Cuts at: 1307 2398 2525 1307
Size: 1091 127 3191
Fragments arranged by size:
3191 1091 127
MspA1I CmG'CkG
Cuts at: 374 635 723 937 963 1070 1520 1834 2194
Size: 261 88 214 26 107 450 314 360
Cuts at: 2194 3207 3452 374
Size: 1013 245 1331
Fragments arranged by size:
1331 1013 450 360 314 261 245 214
107 88 26
NaeI GCC'GGC
Cuts at: 769 893 2590 769
Size: 124 1697 2588
Fragments arranged by size:
2588 1697 124
NarI GG'CG_CC
Cuts at: 932 1057 1207 1840 2088 932
Size: 125 150 633 248 3253
Fragments arranged by size:
3253 633 248 150 125
NcoI C'CATG_G
Cuts at: 1121 1202 1308 1121
Size: 81 106 4222
Fragments arranged by size:
4222 106 81
NgoMIV G'CCGG_C
Cuts at: 767 891 2588 767
Size: 124 1697 2588
Fragments arranged by size:
2588 1697 124
NheI G'CTAG_C
Cuts at: 895 895
Size: 4409
NspI r_CATG'y
Cuts at: 381 1462 1962 2869 381
Size: 1081 500 907 1921
Fragments arranged by size:
1921 1081 907 500
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PflMI CCAn_nnn'nTGG
Cuts at: 1122 1122
Size: 4409
PfoI T'CCnGG_A
Cuts at: 507 507
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PmlI CAC'GTG
Cuts at: 945 945
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 4377
PspOMI G'GGCC_C
Cuts at: 544 544
Size: 4409
PstI C_TGCA'G
Cuts at: 648 1163 1456 2141 648
Size: 515 293 685 2916
Fragments arranged by size:
2916 685 515 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 374 635 723 963 1070 1834 2194 374
Size: 261 88 240 107 764 360 2589
Fragments arranged by size:
2589 764 360 261 240 107 88
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 325 1423 325
Size: 1098 3311
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 1163 1456 1163
Size: 293 4116
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SfcI C'TryA_G
Cuts at: 33 207 234 644 1159 1272 1452 2137 3130
Size: 174 27 410 515 113 180 685 993
Cuts at: 3130 3321 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 515 410 191 180 174
113 27
SfoI GGC'GCC
Cuts at: 933 1058 1208 1841 2089 933
Size: 125 150 633 248 3253
Fragments arranged by size:
3253 633 248 150 125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 1564 2479 2971 3233 1564
Size: 915 492 262 2740
Fragments arranged by size:
2740 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 381 1462 1962 381
Size: 1081 500 2828
Fragments arranged by size:
2828 1081 500
StyI C'CwwG_G
Cuts at: 872 1121 1202 1308 1543 1897 872
Size: 249 81 106 235 354 3384
Fragments arranged by size:
3384 354 249 235 106 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 513 1992 2125 2584 2793 513
Size: 1479 133 459 209 2129
Fragments arranged by size:
2129 1479 459 209 133
TatI w'GTAC_w
Cuts at: 851 3924 851
Size: 3073 1336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 503 535 3926 3958 279
Size: 32 192 32 3391 32 730
Fragments arranged by size:
3391 730 192 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 1904 409
Size: 1495 2914
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI