Restriction map for
pF1KB7730
(Circular) (Six-base) MAPSORT of: pF1KB7730.seq Check: 7933 from: 1 to: 4553
pF1KB7730 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 248 248
Size: 4553
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1374 1428 2378 2810 3557 3825 1374
Size: 54 950 432 747 268 2102
Fragments arranged by size:
2102 950 747 432 268 54
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 808 808
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 225 499 538 1858 3425 225
Size: 274 39 1320 1567 1353
Fragments arranged by size:
1567 1353 1320 274 39
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 121 186 384 716 1573 121
Size: 65 198 332 857 3101
Fragments arranged by size:
3101 857 332 198 65
BamHI G'GATC_C
Cuts at: 1283 1578 1283
Size: 295 4258
BanI G'GyrC_C
Cuts at: 117 817 1036 1144 1187 1419 1569 1983 2231
Size: 700 219 108 43 232 150 414 248
Cuts at: 2231 2266 117
Size: 35 2404
Fragments arranged by size:
2404 700 414 248 232 219 150 108
43 35
BanII G_rGCy'C
Cuts at: 466 571 1567 2597 466
Size: 105 996 1030 2422
Fragments arranged by size:
2422 1030 996 105
BbeI G_GCGC'C
Cuts at: 121 1191 1987 2235 121
Size: 1070 796 248 2439
Fragments arranged by size:
2439 1070 796 248
BbsI GAAGACnn'nnnn_
Cuts at: 240 1125 240
Size: 885 3668
BbvCI CC'TCA_GC
Cuts at: 492 492
Size: 4553
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglI GCCn_nnn'nGGC
Cuts at: 433 512 433
Size: 79 4474
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 820 2178 2271 3327 820
Size: 1358 93 1056 2046
Fragments arranged by size:
2046 1358 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1765 1846 2181 4325 1765
Size: 81 335 2144 1993
Fragments arranged by size:
2144 1993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 535 2920 535
Size: 2385 2168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 521 530 668 918 1479 215
Size: 306 9 138 250 561 3289
Fragments arranged by size:
3289 561 306 250 138 9
Bpu10I CC'TnA_GC
Cuts at: 492 947 492
Size: 455 4098
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2608 3100 3398 1729
Size: 879 492 298 2884
Fragments arranged by size:
2884 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 118 245 478 737 1188 1477 1984 2232 2934
Size: 127 233 259 451 289 507 248 702
Cuts at: 2934 4430 118
Size: 1496 241
Fragments arranged by size:
1496 702 507 451 289 259 248 241
233 127
BsaWI w'CCGG_w
Cuts at: 346 1298 1453 1742 2263 2941 3215 3362 3773
Size: 952 155 289 521 678 274 147 411
Cuts at: 3773 346
Size: 1126
Fragments arranged by size:
1126 952 678 521 411 289 274 155
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 592 622 1477 1507 592
Size: 30 855 30 3638
Fragments arranged by size:
3638 855 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 734 1366 1482 734
Size: 632 116 3805
Fragments arranged by size:
3805 632 116
BseYI C'CCAG_C
Cuts at: 538 821 1978 2736 3313 3809 538
Size: 283 1157 758 577 496 1282
Fragments arranged by size:
1282 1157 758 577 496 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 731 1228 1415 469
Size: 262 497 187 3607
Fragments arranged by size:
3607 497 262 187
BsiEI CG_ry'CG
Cuts at: 104 152 1130 2095 2141 3349 104
Size: 48 978 965 46 1208 1308
Fragments arranged by size:
1308 1208 978 965 48 46
BsiHKAI G_wGCw'C
Cuts at: 571 1567 2345 2535 3327 571
Size: 996 778 190 792 1797
Fragments arranged by size:
1797 996 792 778 190
BsmI GAATG_Cn'
Cuts at: 165 165
Size: 4553
BsmBI CGTCTCn'nnnn_
Cuts at: 321 686 321
Size: 365 4188
Bsp1286I G_dGCh'C
Cuts at: 466 571 820 1567 2178 2271 2345 2535 2597
Size: 105 249 747 611 93 74 190 62
Cuts at: 2597 3327 466
Size: 730 1692
Fragments arranged by size:
1692 747 730 611 249 190 105 93
74 62
BspEI T'CCGG_A
Cuts at: 1453 3773 1453
Size: 2320 2233
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1603 1817 2119 2500 1603
Size: 214 302 381 3656
Fragments arranged by size:
3656 381 302 214
BsrBI CCG'CTC
Cuts at: 50 298 551 2845 2899 4383 4548 50
Size: 248 253 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 253 248 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1062 2465 1062
Size: 1403 3150
BsrFI r'CCGG_y
Cuts at: 867 927 1039 1141 2551 2732 2941 867
Size: 60 112 102 1410 181 209 2479
Fragments arranged by size:
2479 1410 209 181 112 102 60
BssHII G'CGCG_C
Cuts at: 310 310
Size: 4553
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 225 1930 225
Size: 1705 2848
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstEII G'GTnAC_C
Cuts at: 1533 1533
Size: 4553
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4553
BstYI r'GATC_y
Cuts at: 1 1283 1578 2045 2068 2403 2649 3661 3747
Size: 1282 295 467 23 335 246 1012 86
Cuts at: 3747 3759 4105 1
Size: 12 346 449
Fragments arranged by size:
1282 1012 467 449 346 335 295 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 668 698 1541 107
Size: 561 30 843 3119
Fragments arranged by size:
3119 843 561 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 481 1796 481
Size: 1315 3238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 356 391 1044 1079 1206 1241 356
Size: 35 653 35 127 35 3668
Fragments arranged by size:
3668 653 127 35 35 35
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 149 1041 1127 1509 2138 2312 2703 2730 4075
Size: 892 86 382 629 174 391 27 1345
Cuts at: 4075 149
Size: 627
Fragments arranged by size:
1345 892 629 627 391 382 174 86
27
EagI C'GGCC_G
Cuts at: 149 1127 2138 149
Size: 978 1011 2564
Fragments arranged by size:
2564 1011 978
EarI CTCTTCn'nnn_
Cuts at: 904 1131 1318 2576 2786 904
Size: 227 187 1258 210 2671
Fragments arranged by size:
2671 1258 227 210 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1354 2761 3071 3217 4363 1354
Size: 1407 310 146 1146 1544
Fragments arranged by size:
1544 1407 1146 310 146
EcoICRI GAG'CTC
Cuts at: 569 1565 569
Size: 996 3557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 521 530 668 918 1374 1428 1479 2378
Size: 306 9 138 250 456 54 51 899
Cuts at: 2378 2810 3557 3825 215
Size: 432 747 268 943
Fragments arranged by size:
943 899 747 456 432 306 268 250
138 54 51 9
EcoO109I rG'GnC_Cy
Cuts at: 505 1206 1692 505
Size: 701 486 3366
Fragments arranged by size:
3366 701 486
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 164 2334 164
Size: 2170 2383
HaeII r_GCGC'y
Cuts at: 121 1191 1987 2235 3257 4236 121
Size: 1070 796 248 1022 979 438
Fragments arranged by size:
1070 1022 979 796 438 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1088 1120 1275 1307 1342 1366 1374 1398 2395
Size: 32 155 32 35 24 8 24 997
Cuts at: 2395 2425 2427 2457 1088
Size: 30 2 30 3184
Fragments arranged by size:
3184 997 155 35 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 996 1254 1592 996
Size: 258 338 3957
Fragments arranged by size:
3957 338 258
HpaI GTT'AAC
Cuts at: 1254 1254
Size: 4553
KasI G'GCGC_C
Cuts at: 117 1187 1983 2231 117
Size: 1070 796 248 2439
Fragments arranged by size:
2439 1070 796 248
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1498 3224 3408 1334
Size: 164 1726 184 2479
Fragments arranged by size:
2479 1726 184 164
MscI TGG'CCA
Cuts at: 2314 2314
Size: 4553
MslI CAynn'nnrTG
Cuts at: 112 772 2542 2669 112
Size: 660 1770 127 1996
Fragments arranged by size:
1996 1770 660 127
MspA1I CmG'CkG
Cuts at: 222 430 496 563 700 893 1399 1664 1978
Size: 208 66 67 137 193 506 265 314
Cuts at: 1978 2338 3351 3596 222
Size: 360 1013 245 1179
Fragments arranged by size:
1179 1013 506 360 314 265 245 208
193 137 67 66
NaeI GCC'GGC
Cuts at: 869 1143 2734 869
Size: 274 1591 2688
Fragments arranged by size:
2688 1591 274
NarI GG'CG_CC
Cuts at: 118 1188 1984 2232 118
Size: 1070 796 248 2439
Fragments arranged by size:
2439 1070 796 248
NcoI C'CATG_G
Cuts at: 107 1541 107
Size: 1434 3119
NgoMIV G'CCGG_C
Cuts at: 867 1141 2732 867
Size: 274 1591 2688
Fragments arranged by size:
2688 1591 274
NspI r_CATG'y
Cuts at: 1371 1606 2106 3013 1371
Size: 235 500 907 2911
Fragments arranged by size:
2911 907 500 235
PasI CC'CwG_GG
Cuts at: 532 532
Size: 4553
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 538 1184 538
Size: 646 3907
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 1206 1206
Size: 4553
PstI C_TGCA'G
Cuts at: 1600 2285 1600
Size: 685 3868
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 222 496 1399 1978 2338 222
Size: 274 903 579 360 2437
Fragments arranged by size:
2437 903 579 360 274
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 571 1567 571
Size: 996 3557
SacII CC_GC'GG
Cuts at: 701 701
Size: 4553
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SanDI GG'GwC_CC
Cuts at: 1206 1206
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 1131 1318 2576 2786 1131
Size: 187 1258 210 2898
Fragments arranged by size:
2898 1258 210 187
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 746 746
Size: 4553
SfcI C'TryA_G
Cuts at: 33 321 590 885 1596 2281 3274 3465 33
Size: 288 269 295 711 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 295 288 269 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 512 512
Size: 4553
SfoI GGC'GCC
Cuts at: 119 1189 1985 2233 119
Size: 1070 796 248 2439
Fragments arranged by size:
2439 1070 796 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 188 386 1575 188
Size: 198 1189 3166
Fragments arranged by size:
3166 1189 198
SmlI C'TyrA_G
Cuts at: 1708 2623 3115 3377 1708
Size: 915 492 262 2884
Fragments arranged by size:
2884 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1371 1606 2106 1371
Size: 235 500 3818
Fragments arranged by size:
3818 500 235
StuI AGG'CCT
Cuts at: 803 803
Size: 4553
StyI C'CwwG_G
Cuts at: 107 1541 1687 2041 107
Size: 1434 146 354 2619
Fragments arranged by size:
2619 1434 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 645 654 835 2136 2269 2728 2937 645
Size: 9 181 1301 133 459 209 2261
Fragments arranged by size:
2261 1301 459 209 181 133 9
TatI w'GTAC_w
Cuts at: 4068 4068
Size: 4553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 1477 1509 4070 4102 592
Size: 32 853 32 2561 32 1043
Fragments arranged by size:
2561 1043 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 736 1210 2350 736
Size: 474 1140 2939
Fragments arranged by size:
2939 1140 474
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1081 2048 1081
Size: 967 3586
XmaI C'CCGG_G
Cuts at: 186 384 1573 186
Size: 198 1189 3166
Fragments arranged by size:
3166 1189 198
ZraI GAC'GTC
Cuts at: 246 246
Size: 4553
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsrGI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI