Restriction map for
pF1KB7729
(Circular) (Six-base) MAPSORT of: pF1KB7729.seq Check: 8605 from: 1 to: 4406
pF1KB7729 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 306 713 730 2231 2663 3410 3678 230
Size: 76 407 17 1501 432 747 268 958
Fragments arranged by size:
1501 958 747 432 407 268 76 17
AflIII A'CryG_T
Cuts at: 1273 1342 1758 2862 3839 1273
Size: 69 416 1104 977 1840
Fragments arranged by size:
1840 1104 977 416 69
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AhdI GACnn_n'nnGTC
Cuts at: 255 255
Size: 4406
AleI CACnn'nnGTG
Cuts at: 244 244
Size: 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1276 1276
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 160 708 1711 3278 160
Size: 548 1003 1567 1288
Fragments arranged by size:
1567 1288 1003 548
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 518 579 869 1099 1334 1410 3759 4015 518
Size: 61 290 230 235 76 2349 256 909
Fragments arranged by size:
2349 909 290 256 235 230 76 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1154 1187 1154
Size: 33 4373
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 1422 1836 2084 2119 1422
Size: 414 248 35 3709
Fragments arranged by size:
3709 414 248 35
BanII G_rGCy'C
Cuts at: 1298 1420 2450 1298
Size: 122 1030 3254
Fragments arranged by size:
3254 1030 122
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 334 454 1061 1070 334
Size: 120 607 9 3670
Fragments arranged by size:
3670 607 120 9
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BfrBI ATG'CAT
Cuts at: 937 1210 937
Size: 273 4133
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 2031 2124 3180 2031
Size: 93 1056 3257
Fragments arranged by size:
3257 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 944 1618 1699 2034 4178 944
Size: 674 81 335 2144 1172
Fragments arranged by size:
2144 1172 674 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 116
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1122 1759 1122
Size: 637 3769
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 678 1595 2116 2794 3068 3215 3626 678
Size: 917 521 678 274 147 411 1458
Fragments arranged by size:
1458 917 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 368 398 368
Size: 30 4376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 936 1306 936
Size: 370 4036
BseYI C'CCAG_C
Cuts at: 1831 2589 3166 3662 1831
Size: 758 577 496 2575
Fragments arranged by size:
2575 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 488 1298 1420 2198 2388 3180 488
Size: 810 122 778 190 792 1714
Fragments arranged by size:
1714 810 792 778 190 122
Bsp1286I G_dGCh'C
Cuts at: 488 1298 1420 2031 2124 2198 2388 2450 3180
Size: 810 122 611 93 74 190 62 730
Cuts at: 3180 488
Size: 1714
Fragments arranged by size:
1714 810 730 611 190 122 93 74
62
BspEI T'CCGG_A
Cuts at: 678 3626 678
Size: 2948 1458
BspHI T'CATG_A
Cuts at: 294 3582 294
Size: 3288 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1456 1670 1972 2353 1456
Size: 214 302 381 3509
Fragments arranged by size:
3509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 842 907 939 2318 842
Size: 65 32 1379 2930
Fragments arranged by size:
2930 1379 65 32
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 246 572 1330 246
Size: 326 758 3322
Fragments arranged by size:
3322 758 326
BstYI r'GATC_y
Cuts at: 1 1312 1431 1898 1921 2256 2502 3514 3600
Size: 1311 119 467 23 335 246 1012 86
Cuts at: 3600 3612 3958 1
Size: 12 346 449
Fragments arranged by size:
1311 1012 467 449 346 335 246 119
86 23 12
BtgI C'CryG_G
Cuts at: 107 636 107
Size: 529 3877
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 1143 1649 1143
Size: 506 3900
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 975 1010 975
Size: 35 4371
DraI TTT'AAA
Cuts at: 848 1405 848
Size: 557 3849
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 476 1991 2165 2556 2583 3928 476
Size: 1515 174 391 27 1345 954
Fragments arranged by size:
1515 1345 954 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 896 2429 2639 896
Size: 1533 210 2663
Fragments arranged by size:
2663 1533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1296 1418 1296
Size: 122 4284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 230 306 713 730 2231 2663 3410 3678
Size: 114 76 407 17 1501 432 747 268
Cuts at: 3678 116
Size: 844
Fragments arranged by size:
1501 844 747 432 407 268 114 76
17
EcoO109I rG'GnC_Cy
Cuts at: 1545 1545
Size: 4406
EcoRI G'AATT_C
Cuts at: 579 1334 1410 579
Size: 755 76 3575
Fragments arranged by size:
3575 755 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 1840 2088 3110 4089 1840
Size: 248 1022 979 2157
Fragments arranged by size:
2157 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 373 405 1304 1336 1365 1397 2248
Size: 32 100 32 899 32 29 32 851
Cuts at: 2248 2278 2280 2310 241
Size: 30 2 30 2337
Fragments arranged by size:
2337 899 851 100 32 32 32 32
30 30 29 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
HindIII A'AGCT_T
Cuts at: 201 201
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MfeI C'AATT_G
Cuts at: 347 347
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3077 3261 3077
Size: 184 4222
MscI TGG'CCA
Cuts at: 478 2167 478
Size: 1689 2717
MslI CAynn'nnrTG
Cuts at: 244 472 950 2395 2522 244
Size: 228 478 1445 127 2128
Fragments arranged by size:
2128 1445 478 228 127
MspA1I CmG'CkG
Cuts at: 1309 1517 1831 2191 3204 3449 1309
Size: 208 314 360 1013 245 2266
Fragments arranged by size:
2266 1013 360 314 245 208
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 107 636 107
Size: 529 3877
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NsiI A_TGCA'T
Cuts at: 939 1212 939
Size: 273 4133
NspI r_CATG'y
Cuts at: 1277 1346 1459 1959 2866 1277
Size: 69 113 500 907 2817
Fragments arranged by size:
2817 907 500 113 69
PciI A'CATG_T
Cuts at: 1273 1342 2862 1273
Size: 69 1520 2817
Fragments arranged by size:
2817 1520 69
PflMI CCAn_nnn'nTGG
Cuts at: 121 394 121
Size: 273 4133
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PmlI CAC'GTG
Cuts at: 1122 1122
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PstI C_TGCA'G
Cuts at: 1453 2138 1453
Size: 685 3721
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 1309 1831 2191 1309
Size: 522 360 3524
Fragments arranged by size:
3524 522 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1298 1420 1298
Size: 122 4284
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 1365 3923 1365
Size: 2558 1848
SexAI A'CCwGG_T
Cuts at: 791 791
Size: 4406
SfcI C'TryA_G
Cuts at: 33 857 1449 2134 3127 3318 33
Size: 824 592 685 993 191 1121
Fragments arranged by size:
1121 993 824 685 592 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 1561 2476 2968 3230 1561
Size: 915 492 262 2737
Fragments arranged by size:
2737 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 107 636 1540 1894 107
Size: 529 904 354 2619
Fragments arranged by size:
2619 904 529 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 1363 3921 1363
Size: 2558 1848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 368 400 3923 3955 368
Size: 32 3523 32 819
Fragments arranged by size:
3523 819 32 32
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
XmnI GAAnn'nnTTC
Cuts at: 657 1145 657
Size: 488 3918
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PasI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI