Restriction map for
pF1KB7726
(Circular) (Six-base) MAPSORT of: pF1KB7726.seq Check: 7062 from: 1 to: 4553
pF1KB7726 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1388 1388
Size: 4553
AatII G_ACGT'C
Cuts at: 227 227
Size: 4553
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2810 3557 3825 2378
Size: 432 747 268 3106
Fragments arranged by size:
3106 747 432 268
AfeI AGC'GCT
Cuts at: 137 137
Size: 4553
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AleI CACnn'nnGTG
Cuts at: 1519 1519
Size: 4553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 427 1858 3425 427
Size: 1431 1567 1555
Fragments arranged by size:
1567 1555 1431
ApaI G_GGCC'C
Cuts at: 1547 1547
Size: 4553
ApaLI G'TGCA_C
Cuts at: 1156 1522 3323 1156
Size: 366 1801 2386
Fragments arranged by size:
2386 1801 366
ApoI r'AATT_y
Cuts at: 1006 1557 3906 4162 1006
Size: 551 2349 256 1397
Fragments arranged by size:
2349 1397 551 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 349 1537 1573 349
Size: 1188 36 3329
Fragments arranged by size:
3329 1188 36
BamHI G'GATC_C
Cuts at: 212 1578 212
Size: 1366 3187
BanI G'GyrC_C
Cuts at: 1569 1983 2231 2266 1569
Size: 414 248 35 3856
Fragments arranged by size:
3856 414 248 35
BanII G_rGCy'C
Cuts at: 918 987 1200 1547 1567 2597 918
Size: 69 213 347 20 1030 2874
Fragments arranged by size:
2874 1030 347 213 69 20
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 1088 1106 1088
Size: 18 4535
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 1160 1526 1547 2178 2271 3327 1160
Size: 366 21 631 93 1056 2386
Fragments arranged by size:
2386 1056 631 366 93 21
BmgBI CAC'GTC
Cuts at: 177 336 1274 177
Size: 159 938 3456
Fragments arranged by size:
3456 938 159
BmrI ACTGGGnnnn_n'
Cuts at: 647 785 1765 1846 2181 4325 647
Size: 138 980 81 335 2144 875
Fragments arranged by size:
2144 980 875 335 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 792 902 404
Size: 388 110 4055
Fragments arranged by size:
4055 388 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 1375 1729 2608 3100 3398 938
Size: 437 354 879 492 298 2093
Fragments arranged by size:
2093 879 492 437 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 474 1317 1906 474
Size: 843 589 3121
Fragments arranged by size:
3121 843 589
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 224 1984 2232 2934 4430 224
Size: 1760 248 702 1496 347
Fragments arranged by size:
1760 1496 702 347 248
BsaWI w'CCGG_w
Cuts at: 1414 1742 2263 2941 3215 3362 3773 1414
Size: 328 521 678 274 147 411 2194
Fragments arranged by size:
2194 678 521 411 328 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 314 344 558 588 790 820 314
Size: 30 214 30 202 30 4047
Fragments arranged by size:
4047 214 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 928 159
Size: 769 3784
BseYI C'CCAG_C
Cuts at: 138 180 1978 2736 3313 3809 138
Size: 42 1798 758 577 496 882
Fragments arranged by size:
1798 882 758 577 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 919 413
Size: 506 4047
BsiEI CG_ry'CG
Cuts at: 104 449 2095 2141 3349 104
Size: 345 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 345 46
BsiHKAI G_wGCw'C
Cuts at: 261 341 473 1048 1160 1526 1567 2345 2535
Size: 80 132 575 112 366 41 778 190
Cuts at: 2535 3327 261
Size: 792 1487
Fragments arranged by size:
1487 792 778 575 366 190 132 112
80 41
BsmBI CGTCTCn'nnnn_
Cuts at: 791 1264 791
Size: 473 4080
Bsp1286I G_dGCh'C
Cuts at: 261 341 473 918 987 1048 1160 1200 1526
Size: 80 132 445 69 61 112 40 326
Cuts at: 1526 1547 1567 2178 2271 2345 2535 2597 3327
Size: 21 20 611 93 74 190 62 730
Cuts at: 3327 261
Size: 1487
Fragments arranged by size:
1487 730 611 445 326 190 132 112
93 80 74 69 62 61 40 21
20
BspEI T'CCGG_A
Cuts at: 3773 3773
Size: 4553
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1366 1388 1603 1817 2119 2500 1366
Size: 22 215 214 302 381 3419
Fragments arranged by size:
3419 381 302 215 214 22
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2465 2465
Size: 4553
BsrFI r'CCGG_y
Cuts at: 1109 1208 2551 2732 2941 1109
Size: 99 1343 181 209 2721
Fragments arranged by size:
2721 1343 209 181 99
BsrGI T'GTAC_A
Cuts at: 395 1290 395
Size: 895 3658
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstYI r'GATC_y
Cuts at: 1 212 1578 2045 2068 2403 2649 3661 3747
Size: 211 1366 467 23 335 246 1012 86
Cuts at: 3747 3759 4105 1
Size: 12 346 449
Fragments arranged by size:
1366 1012 467 449 346 335 246 211
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 261 2503 2684 2847 261
Size: 2242 181 163 1967
Fragments arranged by size:
2242 1967 181 163
BtsI GCAGTG_nn'
Cuts at: 291 425 846 1179 1431 1796 291
Size: 134 421 333 252 365 3048
Fragments arranged by size:
3048 421 365 333 252 134
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 446 1210 2138 2312 2703 2730 4075 446
Size: 764 928 174 391 27 1345 924
Fragments arranged by size:
1345 928 924 764 391 174 27
EagI C'GGCC_G
Cuts at: 446 2138 446
Size: 1692 2861
EarI CTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1363 2761 3071 3217 4363 1363
Size: 1398 310 146 1146 1553
Fragments arranged by size:
1553 1398 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1565 1565
Size: 4553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 792 902 2378 2810 3557 3825 404
Size: 388 110 1476 432 747 268 1132
Fragments arranged by size:
1476 1132 747 432 388 268 110
EcoNI CCTnn'n_nnAGG
Cuts at: 578 578
Size: 4553
EcoO109I rG'GnC_Cy
Cuts at: 355 646 850 1543 1692 355
Size: 291 204 693 149 3216
Fragments arranged by size:
3216 693 291 204 149
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 2334 2334
Size: 4553
HaeII r_GCGC'y
Cuts at: 139 597 1987 2235 3257 4236 139
Size: 458 1390 248 1022 979 456
Fragments arranged by size:
1390 1022 979 458 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 790 822 2395 2425 2427 2457 325
Size: 32 433 32 1573 30 2 30 2421
Fragments arranged by size:
2421 1573 433 32 32 30 30 2
HincII GTy'rAC
Cuts at: 766 1471 1592 766
Size: 705 121 3727
Fragments arranged by size:
3727 705 121
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 907 3224 3408 197
Size: 710 2317 184 1342
Fragments arranged by size:
2317 1342 710 184
MscI TGG'CCA
Cuts at: 2314 2314
Size: 4553
MslI CAynn'nnrTG
Cuts at: 1519 2542 2669 1519
Size: 1023 127 3403
Fragments arranged by size:
3403 1023 127
MspA1I CmG'CkG
Cuts at: 1016 1104 1664 1978 2338 3351 3596 1016
Size: 88 560 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 560 360 314 245 88
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NcoI C'CATG_G
Cuts at: 107 107
Size: 4553
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NotI GC'GGCC_GC
Cuts at: 446 446
Size: 4553
NspI r_CATG'y
Cuts at: 488 1606 2106 3013 488
Size: 1118 500 907 2028
Fragments arranged by size:
2028 1118 907 500
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PmlI CAC'GTG
Cuts at: 474 1317 474
Size: 843 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 355 850 355
Size: 495 4058
PspOMI G'GGCC_C
Cuts at: 1543 1543
Size: 4553
PstI C_TGCA'G
Cuts at: 286 640 1385 1600 2285 286
Size: 354 745 215 685 2554
Fragments arranged by size:
2554 745 685 354 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 1016 1104 1978 2338 1016
Size: 88 874 360 3231
Fragments arranged by size:
3231 874 360 88
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 1567 1567
Size: 4553
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SanDI GG'GwC_CC
Cuts at: 355 355
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 579 579
Size: 4553
SfcI C'TryA_G
Cuts at: 33 282 636 1381 1596 2281 3274 3465 33
Size: 249 354 745 215 685 993 191 1121
Fragments arranged by size:
1121 993 745 685 354 249 215 191
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 351 1575 351
Size: 1224 3329
SmlI C'TyrA_G
Cuts at: 953 1390 1708 2623 3115 3377 953
Size: 437 318 915 492 262 2129
Fragments arranged by size:
2129 915 492 437 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 488 1606 2106 488
Size: 1118 500 2935
Fragments arranged by size:
2935 1118 500
StuI AGG'CCT
Cuts at: 679 724 805 679
Size: 45 81 4427
Fragments arranged by size:
4427 81 45
StyI C'CwwG_G
Cuts at: 107 674 1064 1687 2041 107
Size: 567 390 623 354 2619
Fragments arranged by size:
2619 623 567 390 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 623 2136 2269 2728 2937 623
Size: 1513 133 459 209 2239
Fragments arranged by size:
2239 1513 459 209 133
TatI w'GTAC_w
Cuts at: 395 695 701 1290 4068 395
Size: 300 6 589 2778 880
Fragments arranged by size:
2778 880 589 300 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 314 346 422 454 4070 4102 314
Size: 32 76 32 3616 32 765
Fragments arranged by size:
3616 765 76 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 2048
Size: 4553
XmaI C'CCGG_G
Cuts at: 349 1573 349
Size: 1224 3329
ZraI GAC'GTC
Cuts at: 225 225
Size: 4553
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BglI BmtI Bpu10I BsiWI BsmI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI