Restriction map for
pF1KB7725
(Circular) (Six-base) MAPSORT of: pF1KB7725.seq Check: 6405 from: 1 to: 4382
pF1KB7725 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 418 1398 418
Size: 980 3402
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 720 804 1056 2207 2639 3386 3654 552
Size: 168 84 252 1151 432 747 268 1280
Fragments arranged by size:
1280 1151 747 432 268 252 168 84
AflIII A'CryG_T
Cuts at: 1132 1734 2838 3815 1132
Size: 602 1104 977 1699
Fragments arranged by size:
1699 1104 977 602
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AleI CACnn'nnGTG
Cuts at: 1058 1058
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 806 1687 3254 806
Size: 881 1567 1934
Fragments arranged by size:
1934 1567 881
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 407 854 1386 3735 3991 407
Size: 447 532 2349 256 798
Fragments arranged by size:
2349 798 532 447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
AvrII C'CTAG_G
Cuts at: 352 352
Size: 4382
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 418 1398 1812 2060 2095 418
Size: 980 414 248 35 2705
Fragments arranged by size:
2705 980 414 248 35
BanII G_rGCy'C
Cuts at: 188 1396 2426 188
Size: 1208 1030 2144
Fragments arranged by size:
2144 1208 1030
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 558 558
Size: 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 463 497 463
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BfrBI ATG'CAT
Cuts at: 1362 1362
Size: 4382
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4382
BglII A'GATC_T
Cuts at: 661 745 1897 3934 661
Size: 84 1152 2037 1109
Fragments arranged by size:
2037 1152 1109 84
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmgBI CAC'GTC
Cuts at: 297 297
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 340 1594 1675 2010 4154 340
Size: 1254 81 335 2144 568
Fragments arranged by size:
2144 1254 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 427 553 1019 2749 427
Size: 126 466 1730 2060
Fragments arranged by size:
2060 1730 466 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 391 632 884 1136 325
Size: 66 241 252 252 3571
Fragments arranged by size:
3571 252 252 241 66
Bpu10I CC'TnA_GC
Cuts at: 467 467
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1558 2437 2929 3227 870
Size: 688 879 492 298 2025
Fragments arranged by size:
2025 879 688 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 161 191 161
Size: 30 4352
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 1092 265
Size: 827 3555
BseYI C'CCAG_C
Cuts at: 365 1807 2565 3142 3638 365
Size: 1442 758 577 496 1109
Fragments arranged by size:
1442 1109 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1239
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 1396 2174 2364 3156 188
Size: 1208 778 190 792 1414
Fragments arranged by size:
1414 1208 792 778 190
BsmI GAATG_Cn'
Cuts at: 718 802 1022 1054 1364 718
Size: 84 220 32 310 3736
Fragments arranged by size:
3736 310 220 84 32
Bsp1286I G_dGCh'C
Cuts at: 188 1396 2007 2100 2174 2364 2426 3156 188
Size: 1208 611 93 74 190 62 730 1414
Fragments arranged by size:
1414 1208 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 259 723 2294 259
Size: 464 1571 2347
Fragments arranged by size:
2347 1571 464
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BsrGI T'GTAC_A
Cuts at: 287 287
Size: 4382
BssSI C'ACGA_G
Cuts at: 993 2653 3011 993
Size: 1660 358 2364
Fragments arranged by size:
2364 1660 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 483 2743 483
Size: 2260 2122
BstEII G'GTnAC_C
Cuts at: 213 213
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 1060 1060
Size: 4382
BstYI r'GATC_y
Cuts at: 1 661 745 1407 1874 1897 2232 2478 3490
Size: 660 84 662 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 662 660 467 449 346 335 246
86 84 23 12
BtgI C'CryG_G
Cuts at: 107 233 107
Size: 126 4256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 639 807 1227 1625 639
Size: 168 420 398 3396
Fragments arranged by size:
3396 420 398 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 705 740 705
Size: 35 4347
DraI TTT'AAA
Cuts at: 447 561 1381 447
Size: 114 820 3448
Fragments arranged by size:
3448 820 114
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 230 1967 2141 2532 2559 3904 230
Size: 1737 174 391 27 1345 708
Fragments arranged by size:
1737 1345 708 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 392 2405 2615 392
Size: 2013 210 2159
Fragments arranged by size:
2159 2013 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 2590 2900 3046 4192 182
Size: 2408 310 146 1146 372
Fragments arranged by size:
2408 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 186 1394 186
Size: 1208 3174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 391 552 632 720 804 884 1056 1136
Size: 66 161 80 88 84 80 172 80
Cuts at: 1136 2207 2639 3386 3654 325
Size: 1071 432 747 268 1053
Fragments arranged by size:
1071 1053 747 432 268 172 161 88
84 80 80 80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 1074 1074
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 1521 1521
Size: 4382
EcoRI G'AATT_C
Cuts at: 407 854 1386 407
Size: 447 532 3403
Fragments arranged by size:
3403 532 447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 507 539 1071 1103 2224 2254 2256 2286 507
Size: 32 532 32 1121 30 2 30 2603
Fragments arranged by size:
2603 1121 532 32 32 30 30 2
HincII GTy'rAC
Cuts at: 461 1421 461
Size: 960 3422
HpaI GTT'AAC
Cuts at: 461 461
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 422 1402 422
Size: 980 3402
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 1058 2371 2498 1058
Size: 1313 127 2942
Fragments arranged by size:
2942 1313 127
MspA1I CmG'CkG
Cuts at: 369 1493 1807 2167 3180 3425 369
Size: 1124 314 360 1013 245 1326
Fragments arranged by size:
1326 1124 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NsiI A_TGCA'T
Cuts at: 1364 1364
Size: 4382
NspI r_CATG'y
Cuts at: 478 1136 1435 1935 2842 478
Size: 658 299 500 907 2018
Fragments arranged by size:
2018 907 658 500 299
PciI A'CATG_T
Cuts at: 1132 2838 1132
Size: 1706 2676
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 310 342 3521 3553 310
Size: 32 3179 32 1139
Fragments arranged by size:
3179 1139 32 32
PstI C_TGCA'G
Cuts at: 316 634 1429 2114 316
Size: 318 795 685 2584
Fragments arranged by size:
2584 795 685 318
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 369 1807 2167 369
Size: 1438 360 2584
Fragments arranged by size:
2584 1438 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 188 1396 188
Size: 1208 3174
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 312 630 651 1017 1425 2110 3103 3294
Size: 279 318 21 366 408 685 993 191
Cuts at: 3294 33
Size: 1121
Fragments arranged by size:
1121 993 685 408 366 318 279 191
21
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 849 1537 2452 2944 3206 849
Size: 688 915 492 262 2025
Fragments arranged by size:
2025 915 688 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 352 1516 1870 107
Size: 245 1164 354 2619
Fragments arranged by size:
2619 1164 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 1965 2098 2557 2766 259
Size: 1706 133 459 209 1875
Fragments arranged by size:
1875 1706 459 209 133
TatI w'GTAC_w
Cuts at: 287 3897 287
Size: 3610 772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 496 1413 496
Size: 917 3465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 1877
Size: 4382
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
XmnI GAAnn'nnTTC
Cuts at: 733 817 1321 733
Size: 84 504 3794
Fragments arranged by size:
3794 504 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbvCI BmtI BsiWI BsmBI BssHII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI