Restriction map for
pF1KB7723
(Circular) (Six-base) MAPSORT of: pF1KB7723.seq Check: 4476 from: 1 to: 4403
pF1KB7723 4403 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 805 805
Size: 4403
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 1397 4243 1397
Size: 2846 1557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 843 1328 2228 2660 3407 3675 805
Size: 38 485 900 432 747 268 1533
Fragments arranged by size:
1533 900 747 485 432 268 38
AflIII A'CryG_T
Cuts at: 1169 1755 2859 3836 1169
Size: 586 1104 977 1736
Fragments arranged by size:
1736 1104 977 586
AgeI A'CCGG_T
Cuts at: 2791 2791
Size: 4403
AlwNI CAG_nnn'CTG
Cuts at: 309 526 535 544 1135 1162 1708 3275 309
Size: 217 9 9 591 27 546 1567 1437
Fragments arranged by size:
1567 1437 591 546 217 27 9 9
ApaI G_GGCC'C
Cuts at: 190 497 190
Size: 307 4096
ApaLI G'TGCA_C
Cuts at: 3173 3173
Size: 4403
ApoI r'AATT_y
Cuts at: 1407 3756 4012 1407
Size: 2349 256 1798
Fragments arranged by size:
2349 1798 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 240 496 1270 1423 240
Size: 256 774 153 3220
Fragments arranged by size:
3220 774 256 153
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 1419 1833 2081 2116 1419
Size: 414 248 35 3706
Fragments arranged by size:
3706 414 248 35
BanII G_rGCy'C
Cuts at: 190 421 497 1193 1417 2447 190
Size: 231 76 696 224 1030 2146
Fragments arranged by size:
2146 1030 696 231 224 76
BbeI G_GCGC'C
Cuts at: 1837 2085 1837
Size: 248 4155
BbvCI CC'TCA_GC
Cuts at: 406 688 406
Size: 282 4121
BciVI GTATCCnnnnn_n'
Cuts at: 2301 3062 4403 2301
Size: 761 1341 2301
Fragments arranged by size:
2301 1341 761
BclI T'GATC_A
Cuts at: 1923 1923
Size: 4403
BglI GCCn_nnn'nGGC
Cuts at: 155 155
Size: 4403
BglII A'GATC_T
Cuts at: 1918 3955 1918
Size: 2037 2366
BlpI GC'TnA_GC
Cuts at: 513 1515 513
Size: 1002 3401
Bme1580I G_kGCm'C
Cuts at: 190 497 2028 2121 3177 190
Size: 307 1531 93 1056 1416
Fragments arranged by size:
1531 1416 1056 307 93
BmrI ACTGGGnnnn_n'
Cuts at: 133 1615 1696 2031 4175 133
Size: 1482 81 335 2144 361
Fragments arranged by size:
2144 1482 361 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 655 1184 122
Size: 533 529 3341
Fragments arranged by size:
3341 533 529
Bpu10I CC'TnA_GC
Cuts at: 406 688 406
Size: 282 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 2458 2950 3248 1579
Size: 879 492 298 2734
Fragments arranged by size:
2734 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 206 984 4107 206
Size: 778 3123 502
Fragments arranged by size:
3123 778 502
BsaAI yAC'GTr
Cuts at: 1756 1756
Size: 4403
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 1834 2082 2784 4280 1834
Size: 248 702 1496 1957
Fragments arranged by size:
1957 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1592 2113 2791 3065 3212 3623 1592
Size: 521 678 274 147 411 2372
Fragments arranged by size:
2372 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 445 943 955 1054 442
Size: 3 498 12 99 3791
Fragments arranged by size:
3791 498 99 12 3
BseYI C'CCAG_C
Cuts at: 490 1828 2586 3163 3659 490
Size: 1338 758 577 496 1234
Fragments arranged by size:
1338 1234 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1945 1991 3199 104
Size: 1841 46 1208 1308
Fragments arranged by size:
1841 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 421 796 1417 2195 2385 3177 421
Size: 375 621 778 190 792 1647
Fragments arranged by size:
1647 792 778 621 375 190
BsmI GAATG_Cn'
Cuts at: 410 410
Size: 4403
Bsp1286I G_dGCh'C
Cuts at: 190 421 497 796 1193 1417 2028 2121 2195
Size: 231 76 299 397 224 611 93 74
Cuts at: 2195 2385 2447 3177 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 397 299 231 224 190
93 76 74 62
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 677 3579 677
Size: 2902 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 805 1453 1667 1969 2350 692
Size: 113 648 214 302 381 2745
Fragments arranged by size:
2745 648 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 2695 2749 4233 4398 50
Size: 2645 54 1484 165 55
Fragments arranged by size:
2645 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2315 2315
Size: 4403
BsrFI r'CCGG_y
Cuts at: 435 2401 2582 2791 435
Size: 1966 181 209 2047
Fragments arranged by size:
2047 1966 209 181
BsrGI T'GTAC_A
Cuts at: 1286 1286
Size: 4403
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 526 535 1780 526
Size: 9 1245 3149
Fragments arranged by size:
3149 1245 9
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 714 729 714
Size: 15 4388
BstYI r'GATC_y
Cuts at: 1 608 1159 1428 1895 1918 2253 2499 3511
Size: 607 551 269 467 23 335 246 1012
Cuts at: 3511 3597 3609 3955 1
Size: 86 12 346 449
Fragments arranged by size:
1012 607 551 467 449 346 335 269
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 713 1294 107
Size: 606 581 3216
Fragments arranged by size:
3216 606 581
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1646
Size: 4403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 939 974 939
Size: 35 4368
DraI TTT'AAA
Cuts at: 1402 1402
Size: 4403
DrdI GACnn_nn'nnGTC
Cuts at: 1071 2109 2967 1071
Size: 1038 858 2507
Fragments arranged by size:
2507 1038 858
EaeI y'GGCC_r
Cuts at: 1988 2162 2553 2580 3925 1988
Size: 174 391 27 1345 2466
Fragments arranged by size:
2466 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 1347 2426 2636 1347
Size: 1079 210 3114
Fragments arranged by size:
3114 1079 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 2611 2921 3067 4213 224
Size: 2387 310 146 1146 414
Fragments arranged by size:
2387 1146 414 310 146
EcoICRI GAG'CTC
Cuts at: 419 1415 419
Size: 996 3407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 655 805 843 1184 1328 2228 2660 3407
Size: 533 150 38 341 144 900 432 747
Cuts at: 3407 3675 122
Size: 268 850
Fragments arranged by size:
900 850 747 533 432 341 268 150
144 38
EcoNI CCTnn'n_nnAGG
Cuts at: 313 313
Size: 4403
EcoO109I rG'GnC_Cy
Cuts at: 156 186 187 237 879 1542 156
Size: 30 1 50 642 663 3017
Fragments arranged by size:
3017 663 642 50 30 1
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4403
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 770 852 1837 2085 3107 4086 770
Size: 82 985 248 1022 979 1087
Fragments arranged by size:
1087 1022 985 979 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2245 2275 2277 2307 2245
Size: 30 2 30 4341
Fragments arranged by size:
4341 30 30 2
HincII GTy'rAC
Cuts at: 1442 1442
Size: 4403
KasI G'GCGC_C
Cuts at: 1833 2081 1833
Size: 248 4155
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1007 1257 1418 3074 3258 1007
Size: 250 161 1656 184 2152
Fragments arranged by size:
2152 1656 250 184 161
MscI TGG'CCA
Cuts at: 2164 2164
Size: 4403
MslI CAynn'nnrTG
Cuts at: 775 967 2392 2519 775
Size: 192 1425 127 2659
Fragments arranged by size:
2659 1425 192 127
MspA1I CmG'CkG
Cuts at: 450 526 535 783 1319 1514 1828 2188 3201
Size: 76 9 248 536 195 314 360 1013
Cuts at: 3201 3446 450
Size: 245 1407
Fragments arranged by size:
1407 1013 536 360 314 248 245 195
76 9
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 4403
NarI GG'CG_CC
Cuts at: 1834 2082 1834
Size: 248 4155
NcoI C'CATG_G
Cuts at: 107 107
Size: 4403
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 4403
NspI r_CATG'y
Cuts at: 471 656 1173 1456 1956 2863 471
Size: 185 517 283 500 907 2011
Fragments arranged by size:
2011 907 517 500 283 185
PasI CC'CwG_GG
Cuts at: 1083 1083
Size: 4403
PciI A'CATG_T
Cuts at: 1169 2859 1169
Size: 1690 2713
PflMI CCAn_nnn'nTGG
Cuts at: 309 309
Size: 4403
PfoI T'CCnGG_A
Cuts at: 830 830
Size: 4403
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PpuMI rG'GwC_Cy
Cuts at: 237 879 237
Size: 642 3761
PshAI GACnn'nnGTC
Cuts at: 673 673
Size: 4403
PspOMI G'GGCC_C
Cuts at: 186 493 186
Size: 307 4096
PstI C_TGCA'G
Cuts at: 281 531 540 1131 1450 2135 281
Size: 250 9 591 319 685 2549
Fragments arranged by size:
2549 685 591 319 250 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 450 526 783 1319 1828 2188 450
Size: 76 257 536 509 360 2665
Fragments arranged by size:
2665 536 509 360 257 76
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 421 1417 421
Size: 996 3407
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SanDI GG'GwC_CC
Cuts at: 237 237
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SfcI C'TryA_G
Cuts at: 33 277 527 536 1127 1446 2131 3124 3315
Size: 244 250 9 591 319 685 993 191
Cuts at: 3315 33
Size: 1121
Fragments arranged by size:
1121 993 685 591 319 250 244 191
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 155 155
Size: 4403
SfoI GGC'GCC
Cuts at: 1835 2083 1835
Size: 248 4155
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 242 1425 242
Size: 1183 3220
SmlI C'TyrA_G
Cuts at: 1558 2473 2965 3227 1558
Size: 915 492 262 2734
Fragments arranged by size:
2734 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
StyI C'CwwG_G
Cuts at: 107 935 1537 1891 107
Size: 828 602 354 2619
Fragments arranged by size:
2619 828 602 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1096 1986 2119 2578 2787 1096
Size: 890 133 459 209 2712
Fragments arranged by size:
2712 890 459 209 133
TatI w'GTAC_w
Cuts at: 1286 3918 1286
Size: 2632 1771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4371
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1434 1434
Size: 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1898 1898
Size: 4403
XmaI C'CCGG_G
Cuts at: 240 1423 240
Size: 1183 3220
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI