Restriction map for
pF1KB7715
(Circular) (Six-base) MAPSORT of: pF1KB7715.seq Check: 8424 from: 1 to: 4391
pF1KB7715 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 781 891 781
Size: 110 4281
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2648 3395 3663 2216
Size: 432 747 268 2944
Fragments arranged by size:
2944 747 432 268
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 398 2779 398
Size: 2381 2010
AleI CACnn'nnGTG
Cuts at: 661 661
Size: 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 679 1162 679
Size: 483 3908
AlwNI CAG_nnn'CTG
Cuts at: 147 1696 3263 147
Size: 1549 1567 1275
Fragments arranged by size:
1567 1549 1275
ApaI G_GGCC'C
Cuts at: 839 839
Size: 4391
ApaLI G'TGCA_C
Cuts at: 3161 3161
Size: 4391
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 778 838 1288 1411 778
Size: 60 450 123 3758
Fragments arranged by size:
3758 450 123 60
BamHI G'GATC_C
Cuts at: 218 1416 218
Size: 1198 3193
BanI G'GyrC_C
Cuts at: 820 923 1407 1821 2069 2104 820
Size: 103 484 414 248 35 3107
Fragments arranged by size:
3107 484 414 248 103 35
BanII G_rGCy'C
Cuts at: 376 595 839 1405 2435 376
Size: 219 244 566 1030 2332
Fragments arranged by size:
2332 1030 566 244 219
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbsI GAAGACnn'nnnn_
Cuts at: 349 1062 1305 349
Size: 713 243 3435
Fragments arranged by size:
3435 713 243
BciVI GTATCCnnnnn_n'
Cuts at: 185 2289 3050 4391 185
Size: 2104 761 1341 185
Fragments arranged by size:
2104 1341 761 185
BclI T'GATC_A
Cuts at: 545 581 725 1911 545
Size: 36 144 1186 3025
Fragments arranged by size:
3025 1186 144 36
BglI GCCn_nnn'nGGC
Cuts at: 782 962 782
Size: 180 4211
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 839 926 2016 2109 3165 839
Size: 87 1090 93 1056 2065
Fragments arranged by size:
2065 1090 1056 93 87
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 949 1066 868
Size: 81 117 4193
Fragments arranged by size:
4193 117 81
Bpu10I CC'TnA_GC
Cuts at: 799 799
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2446 2938 3236 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 251 724 1905 3942 6
Size: 245 473 1181 2037 455
Fragments arranged by size:
2037 1181 473 455 245
BsaHI Gr'CG_yC
Cuts at: 875 1822 2070 2772 4268 875
Size: 947 248 702 1496 998
Fragments arranged by size:
1496 998 947 702 248
BsaWI w'CCGG_w
Cuts at: 116 215 398 1170 1316 1580 2101 2779 3053
Size: 99 183 772 146 264 521 678 274
Cuts at: 3053 3200 3611 116
Size: 147 411 896
Fragments arranged by size:
896 772 678 521 411 274 264 183
147 146 99
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 1306 214
Size: 1092 3299
BseYI C'CCAG_C
Cuts at: 652 883 1249 1816 2574 3151 3647 652
Size: 231 366 567 758 577 496 1396
Fragments arranged by size:
1396 758 577 567 496 366 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1228 931
Size: 297 4094
BsiEI CG_ry'CG
Cuts at: 104 525 872 1933 1979 3187 104
Size: 421 347 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 421 347 46
BsiHKAI G_wGCw'C
Cuts at: 1405 2183 2373 3165 1405
Size: 778 190 792 2631
Fragments arranged by size:
2631 792 778 190
BsmI GAATG_Cn'
Cuts at: 709 709
Size: 4391
Bsp1286I G_dGCh'C
Cuts at: 376 595 839 926 1405 2016 2109 2183 2373
Size: 219 244 87 479 611 93 74 190
Cuts at: 2373 2435 3165 376
Size: 62 730 1602
Fragments arranged by size:
1602 730 611 479 244 219 190 93
87 74 62
BspEI T'CCGG_A
Cuts at: 215 1170 1316 3611 215
Size: 955 146 2295 995
Fragments arranged by size:
2295 995 955 146
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 781 891 1441 1655 1957 2338 781
Size: 110 550 214 302 381 2834
Fragments arranged by size:
2834 550 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 398 867 956 1124 1267 2389 2570 2779 398
Size: 469 89 168 143 1122 181 209 2010
Fragments arranged by size:
2010 1122 469 209 181 168 143 89
BssSI C'ACGA_G
Cuts at: 1204 2662 3020 1204
Size: 1458 358 2575
Fragments arranged by size:
2575 1458 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 338 638 1044 338
Size: 300 406 3685
Fragments arranged by size:
3685 406 300
BstYI r'GATC_y
Cuts at: 1 218 1416 1883 1906 2241 2487 3499 3585
Size: 217 1198 467 23 335 246 1012 86
Cuts at: 3585 3597 3943 1
Size: 12 346 449
Fragments arranged by size:
1198 1012 467 449 346 335 246 217
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 449 449
Size: 4391
BtgI C'CryG_G
Cuts at: 1016 1016
Size: 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 1634 1634
Size: 4391
DraI TTT'AAA
Cuts at: 297 309 1390 297
Size: 12 1081 3298
Fragments arranged by size:
3298 1081 12
DrdI GACnn_nn'nnGTC
Cuts at: 399 2097 2955 399
Size: 1698 858 1835
Fragments arranged by size:
1835 1698 858
EaeI y'GGCC_r
Cuts at: 869 1976 2150 2541 2568 3913 869
Size: 1107 174 391 27 1345 1347
Fragments arranged by size:
1347 1345 1107 391 174 27
EagI C'GGCC_G
Cuts at: 869 1976 869
Size: 1107 3284
EarI CTCTTCn'nnn_
Cuts at: 199 449 1157 1238 1249 2414 2624 199
Size: 250 708 81 11 1165 210 1966
Fragments arranged by size:
1966 1165 708 250 210 81 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 2909 3055 4201 2599
Size: 310 146 1146 2789
Fragments arranged by size:
2789 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 949 1066 2216 2648 3395 3663 868
Size: 81 117 1150 432 747 268 1596
Fragments arranged by size:
1596 1150 747 432 268 117 81
EcoO109I rG'GnC_Cy
Cuts at: 843 1284 1530 843
Size: 441 246 3704
Fragments arranged by size:
3704 441 246
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 611 2410 611
Size: 1799 2592
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 1825 2073 3095 4074 1825
Size: 248 1022 979 2142
Fragments arranged by size:
2142 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2233 2263 2265 2295 2233
Size: 30 2 30 4329
Fragments arranged by size:
4329 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 595 3062 3246 595
Size: 2467 184 1740
Fragments arranged by size:
2467 1740 184
MscI TGG'CCA
Cuts at: 2152 2152
Size: 4391
MslI CAynn'nnrTG
Cuts at: 577 661 1204 2380 2507 577
Size: 84 543 1176 127 2461
Fragments arranged by size:
2461 1176 543 127 84
MspA1I CmG'CkG
Cuts at: 669 1007 1221 1502 1816 2176 3189 3434 669
Size: 338 214 281 314 360 1013 245 1626
Fragments arranged by size:
1626 1013 360 338 314 281 245 214
NaeI GCC'GGC
Cuts at: 869 958 1126 1269 2572 869
Size: 89 168 143 1303 2688
Fragments arranged by size:
2688 1303 168 143 89
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NgoMIV G'CCGG_C
Cuts at: 867 956 1124 1267 2570 867
Size: 89 168 143 1303 2688
Fragments arranged by size:
2688 1303 168 143 89
NspI r_CATG'y
Cuts at: 174 213 1444 1944 2851 174
Size: 39 1231 500 907 1714
Fragments arranged by size:
1714 1231 907 500 39
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PfoI T'CCnGG_A
Cuts at: 177 177
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 4359
PsiI TTA'TAA
Cuts at: 251 251
Size: 4391
PspOMI G'GGCC_C
Cuts at: 835 835
Size: 4391
PstI C_TGCA'G
Cuts at: 1006 1103 1438 2123 1006
Size: 97 335 685 3274
Fragments arranged by size:
3274 685 335 97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1221 1816 2176 1221
Size: 595 360 3436
Fragments arranged by size:
3436 595 360
RsrII CG'GwC_CG
Cuts at: 396 2586 396
Size: 2190 2201
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 199 449 1249 2414 2624 199
Size: 250 800 1165 210 1966
Fragments arranged by size:
1966 1165 800 250 210
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SexAI A'CCwGG_T
Cuts at: 1307 1307
Size: 4391
SfcI C'TryA_G
Cuts at: 33 1002 1099 1434 2119 3112 3303 33
Size: 969 97 335 685 993 191 1121
Fragments arranged by size:
1121 993 969 685 335 191 97
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 1546 2461 2953 3215 1546
Size: 915 492 262 2722
Fragments arranged by size:
2722 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
StuI AGG'CCT
Cuts at: 283 610 283
Size: 327 4064
StyI C'CwwG_G
Cuts at: 223 1525 1879 223
Size: 1302 354 2735
Fragments arranged by size:
2735 1302 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 3906 3906
Size: 4391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1245 1277 3908 3940 1245
Size: 32 2631 32 1696
Fragments arranged by size:
2631 1696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1309 2188 1309
Size: 879 3512
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 185 1886 185
Size: 1701 2690
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 916 1166 916
Size: 250 4141
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI