Restriction map for
pF1KB7711
(Circular) (Six-base) MAPSORT of: pF1KB7711.seq Check: 9036 from: 1 to: 4376
pF1KB7711 4376 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1414 1414
Size: 4376
Acc65I G'GTAC_C
Cuts at: 1392 1392
Size: 4376
AclI AA'CG_TT
Cuts at: 4216 4216
Size: 4376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2633 3380 3648 2201
Size: 432 747 268 2929
Fragments arranged by size:
2929 747 432 268
AfeI AGC'GCT
Cuts at: 807 807
Size: 4376
AflIII A'CryG_T
Cuts at: 1128 1728 2832 3809 1128
Size: 600 1104 977 1695
Fragments arranged by size:
1695 1104 977 600
AgeI A'CCGG_T
Cuts at: 2764 2764
Size: 4376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 523 1000 523
Size: 477 3899
AlwNI CAG_nnn'CTG
Cuts at: 222 855 858 939 1283 1681 3248 222
Size: 633 3 81 344 398 1567 1350
Fragments arranged by size:
1567 1350 633 398 344 81 3
ApaLI G'TGCA_C
Cuts at: 390 1082 3146 390
Size: 692 2064 1620
Fragments arranged by size:
2064 1620 692
ApoI r'AATT_y
Cuts at: 229 606 1380 3729 3985 229
Size: 377 774 2349 256 620
Fragments arranged by size:
2349 774 620 377 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4376
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
AvaI C'yCGr_G
Cuts at: 132 267 319 411 656 815 1396 132
Size: 135 52 92 245 159 581 3112
Fragments arranged by size:
3112 581 245 159 135 92 52
BamHI G'GATC_C
Cuts at: 1401 1401
Size: 4376
BanI G'GyrC_C
Cuts at: 274 300 315 1392 1806 2054 2089 274
Size: 26 15 1077 414 248 35 2561
Fragments arranged by size:
2561 1077 414 248 35 26 15
BanII G_rGCy'C
Cuts at: 185 803 1108 1120 1301 1390 2420 185
Size: 618 305 12 181 89 1030 2141
Fragments arranged by size:
2141 1030 618 305 181 89 12
BbeI G_GCGC'C
Cuts at: 319 1810 2058 319
Size: 1491 248 2637
Fragments arranged by size:
2637 1491 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1156 1190 1156
Size: 34 4342
BciVI GTATCCnnnnn_n'
Cuts at: 2274 3035 4376 2274
Size: 761 1341 2274
Fragments arranged by size:
2274 1341 761
BclI T'GATC_A
Cuts at: 1896 1896
Size: 4376
BglI GCCn_nnn'nGGC
Cuts at: 145 414 511 520 787 1327 145
Size: 269 97 9 267 540 3194
Fragments arranged by size:
3194 540 269 267 97 9
BglII A'GATC_T
Cuts at: 1891 3928 1891
Size: 2037 2339
BlpI GC'TnA_GC
Cuts at: 1102 1488 1102
Size: 386 3990
Bme1580I G_kGCm'C
Cuts at: 277 394 1086 2001 2094 3150 277
Size: 117 692 915 93 1056 1503
Fragments arranged by size:
1503 1056 915 692 117 93
BmgBI CAC'GTC
Cuts at: 383 395 441 1137 383
Size: 12 46 696 3622
Fragments arranged by size:
3622 696 46 12
BmrI ACTGGGnnnn_n'
Cuts at: 699 1047 1356 1588 1669 2004 4148 699
Size: 348 309 232 81 335 2144 927
Fragments arranged by size:
2144 927 348 335 309 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1127 2743 1127
Size: 1616 2760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1108 1117 196
Size: 912 9 3455
Fragments arranged by size:
3455 912 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 2431 2923 3221 1552
Size: 879 492 298 2707
Fragments arranged by size:
2707 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1222 4080 1222
Size: 2858 1518
BsaAI yAC'GTr
Cuts at: 465 1131 1729 465
Size: 666 598 3112
Fragments arranged by size:
3112 666 598
BsaBI GATnn'nnATC
Cuts at: 6 1890 3927 6
Size: 1884 2037 455
Fragments arranged by size:
2037 1884 455
BsaHI Gr'CG_yC
Cuts at: 188 316 1055 1807 2055 2757 4253 188
Size: 128 739 752 248 702 1496 311
Fragments arranged by size:
1496 752 739 702 311 248 128
BsaWI w'CCGG_w
Cuts at: 1565 2086 2764 3038 3185 3596 1565
Size: 521 678 274 147 411 2345
Fragments arranged by size:
2345 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 193 886 1012 166
Size: 27 693 126 3530
Fragments arranged by size:
3530 693 126 27
BseYI C'CCAG_C
Cuts at: 771 802 848 893 915 973 1077 1312 1801
Size: 31 46 45 22 58 104 235 489
Cuts at: 1801 2559 3136 3632 771
Size: 758 577 496 1515
Fragments arranged by size:
1515 758 577 496 489 235 104 58
46 45 31 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 920
Size: 4376
BsiEI CG_ry'CG
Cuts at: 104 338 1158 1918 1964 3172 104
Size: 234 820 760 46 1208 1308
Fragments arranged by size:
1308 1208 820 760 234 46
BsiHKAI G_wGCw'C
Cuts at: 394 1086 1390 2168 2358 3150 394
Size: 692 304 778 190 792 1620
Fragments arranged by size:
1620 792 778 692 304 190
Bsp1286I G_dGCh'C
Cuts at: 185 277 394 803 1086 1108 1120 1301 1390
Size: 92 117 409 283 22 12 181 89
Cuts at: 1390 2001 2094 2168 2358 2420 3150 185
Size: 611 93 74 190 62 730 1411
Fragments arranged by size:
1411 730 611 409 283 190 181 117
93 92 89 74 62 22 12
BspEI T'CCGG_A
Cuts at: 3596 3596
Size: 4376
BspHI T'CATG_A
Cuts at: 3552 3552
Size: 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1346 1426 1640 1942 2323 1346
Size: 80 214 302 381 3399
Fragments arranged by size:
3399 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 717 728 1274 2668 2722 4206 4371 50
Size: 667 11 546 1394 54 1484 165 55
Fragments arranged by size:
1484 1394 667 546 165 55 54 11
BsrDI GCAATG_nn'
Cuts at: 2288 2288
Size: 4376
BsrFI r'CCGG_y
Cuts at: 144 1057 2374 2555 2764 144
Size: 913 1317 181 209 1756
Fragments arranged by size:
1756 1317 913 209 181
BssHII G'CGCG_C
Cuts at: 404 404
Size: 4376
BssSI C'ACGA_G
Cuts at: 2647 3005 2647
Size: 358 4018
BstAPI GCAn_nnn'nTGC
Cuts at: 222 858 1283 1753 222
Size: 636 425 470 2845
Fragments arranged by size:
2845 636 470 425
BstBI TT'CG_AA
Cuts at: 2737 2737
Size: 4376
BstXI CCAn_nnnn'nTGG
Cuts at: 951 951
Size: 4376
BstYI r'GATC_y
Cuts at: 1 591 1401 1868 1891 2226 2472 3484 3570
Size: 590 810 467 23 335 246 1012 86
Cuts at: 3570 3582 3928 1
Size: 12 346 449
Fragments arranged by size:
1012 810 590 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 285 964 1152 107
Size: 178 679 188 3331
Fragments arranged by size:
3331 679 188 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2326 2507 2670 2326
Size: 181 163 4032
Fragments arranged by size:
4032 181 163
BtsI GCAGTG_nn'
Cuts at: 223 1284 1619 223
Size: 1061 335 2980
Fragments arranged by size:
2980 1061 335
DraI TTT'AAA
Cuts at: 1375 1375
Size: 4376
DraIII CAC_nnn'GTG
Cuts at: 300 300
Size: 4376
DrdI GACnn_nn'nnGTC
Cuts at: 2082 2940 2082
Size: 858 3518
EaeI y'GGCC_r
Cuts at: 335 503 542 953 1155 1238 1961 2135 2526
Size: 168 39 411 202 83 723 174 391
Cuts at: 2526 2553 3898 335
Size: 27 1345 813
Fragments arranged by size:
1345 813 723 411 391 202 174 168
83 39 27
EagI C'GGCC_G
Cuts at: 335 1155 1961 335
Size: 820 806 2750
Fragments arranged by size:
2750 820 806
EarI CTCTTCn'nnn_
Cuts at: 2399 2609 2399
Size: 210 4166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 163 571 604 2584 2894 3040 4186 127
Size: 36 408 33 1980 310 146 1146 317
Fragments arranged by size:
1980 1146 408 317 310 146 36 33
EcoICRI GAG'CTC
Cuts at: 1388 1388
Size: 4376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1108 1117 2201 2633 3380 3648 196
Size: 912 9 1084 432 747 268 924
Fragments arranged by size:
1084 924 912 747 432 268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1306 1306
Size: 4376
EcoO109I rG'GnC_Cy
Cuts at: 252 323 423 960 1092 1186 1361 1515 252
Size: 71 100 537 132 94 175 154 3113
Fragments arranged by size:
3113 537 175 154 132 100 94 71
EcoRI G'AATT_C
Cuts at: 229 606 1380 229
Size: 377 774 3225
Fragments arranged by size:
3225 774 377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 702 2395 702
Size: 1693 2683
FspI TGC'GCA
Cuts at: 2157 2157
Size: 4376
HaeII r_GCGC'y
Cuts at: 319 734 809 1810 2058 3080 4059 319
Size: 415 75 1001 248 1022 979 636
Fragments arranged by size:
1022 1001 979 636 415 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2218 2248 2250 2280 2218
Size: 30 2 30 4314
Fragments arranged by size:
4314 30 30 2
HincII GTy'rAC
Cuts at: 1415 1415
Size: 4376
KasI G'GCGC_C
Cuts at: 315 1806 2054 315
Size: 1491 248 2637
Fragments arranged by size:
2637 1491 248
KpnI G_GTAC'C
Cuts at: 1396 1396
Size: 4376
MluI A'CGCG_T
Cuts at: 3809 3809
Size: 4376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1358 3047 3231 1358
Size: 1689 184 2503
Fragments arranged by size:
2503 1689 184
MscI TGG'CCA
Cuts at: 505 955 1240 2137 505
Size: 450 285 897 2744
Fragments arranged by size:
2744 897 450 285
MslI CAynn'nnrTG
Cuts at: 499 505 649 2365 2492 499
Size: 6 144 1716 127 2383
Fragments arranged by size:
2383 1716 144 127 6
MspA1I CmG'CkG
Cuts at: 313 828 855 939 966 1487 1801 2161 3174
Size: 515 27 84 27 521 314 360 1013
Cuts at: 3174 3419 313
Size: 245 1270
Fragments arranged by size:
1270 1013 521 515 360 314 245 84
27 27
NaeI GCC'GGC
Cuts at: 146 1059 2557 146
Size: 913 1498 1965
Fragments arranged by size:
1965 1498 913
NarI GG'CG_CC
Cuts at: 316 1807 2055 316
Size: 1491 248 2637
Fragments arranged by size:
2637 1491 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4376
NgoMIV G'CCGG_C
Cuts at: 144 1057 2555 144
Size: 913 1498 1965
Fragments arranged by size:
1965 1498 913
NspI r_CATG'y
Cuts at: 1429 1929 2836 1429
Size: 500 907 2969
Fragments arranged by size:
2969 907 500
PasI CC'CwG_GG
Cuts at: 1262 1262
Size: 4376
PciI A'CATG_T
Cuts at: 2832 2832
Size: 4376
PmeI GTTT'AAAC
Cuts at: 1375 1375
Size: 4376
PmlI CAC'GTG
Cuts at: 465 1131 465
Size: 666 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 4344
PpuMI rG'GwC_Cy
Cuts at: 323 960 323
Size: 637 3739
PstI C_TGCA'G
Cuts at: 218 227 422 1256 1343 1423 2108 218
Size: 9 195 834 87 80 685 2486
Fragments arranged by size:
2486 834 685 195 87 80 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4376
PvuII CAG'CTG
Cuts at: 855 1801 2161 855
Size: 946 360 3070
Fragments arranged by size:
3070 946 360
RsrII CG'GwC_CG
Cuts at: 2571 2571
Size: 4376
SacI G_AGCT'C
Cuts at: 1390 1390
Size: 4376
SacII CC_GC'GG
Cuts at: 967 967
Size: 4376
SalI G'TCGA_C
Cuts at: 1413 1413
Size: 4376
SanDI GG'GwC_CC
Cuts at: 323 323
Size: 4376
SapI GCTCTTCn'nnn_
Cuts at: 2399 2609 2399
Size: 210 4166
SbfI CC_TGCA'GG
Cuts at: 1343 1423 1343
Size: 80 4296
ScaI AGT'ACT
Cuts at: 3893 3893
Size: 4376
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 4376
SfcI C'TryA_G
Cuts at: 33 214 223 418 1252 1339 1419 2104 3097
Size: 181 9 195 834 87 80 685 993
Cuts at: 3097 3288 33
Size: 191 1121
Fragments arranged by size:
1121 993 834 685 195 191 181 87
80 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 511 520 511
Size: 9 4367
SfoI GGC'GCC
Cuts at: 317 1808 2056 317
Size: 1491 248 2637
Fragments arranged by size:
2637 1491 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
SmaI CCC'GGG
Cuts at: 269 321 413 817 1398 269
Size: 52 92 404 581 3247
Fragments arranged by size:
3247 581 404 92 52
SmlI C'TyrA_G
Cuts at: 1531 2446 2938 3200 1531
Size: 915 492 262 2707
Fragments arranged by size:
2707 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4376
SphI G_CATG'C
Cuts at: 1429 1929 1429
Size: 500 3876
StyI C'CwwG_G
Cuts at: 107 248 956 1510 1864 107
Size: 141 708 554 354 2619
Fragments arranged by size:
2619 708 554 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 785 1069 1959 2092 2551 2760 785
Size: 284 890 133 459 209 2401
Fragments arranged by size:
2401 890 459 284 209 133
TatI w'GTAC_w
Cuts at: 3891 3891
Size: 4376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4344
Tth111I GACn'n_nGTC
Cuts at: 2173 2173
Size: 4376
XbaI T'CTAG_A
Cuts at: 1407 1407
Size: 4376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1871 1871
Size: 4376
XmaI C'CCGG_G
Cuts at: 267 319 411 815 1396 267
Size: 52 92 404 581 3247
Fragments arranged by size:
3247 581 404 92 52
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI