Restriction map for
pF1KB7709
(Circular) (Six-base) MAPSORT of: pF1KB7709.seq Check: 3104 from: 1 to: 4373
pF1KB7709 4373 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1208 1411 1208
Size: 203 4170
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 2198 2630 3377 3645 181
Size: 2017 432 747 268 909
Fragments arranged by size:
2017 909 747 432 268
AflIII A'CryG_T
Cuts at: 1725 2829 3806 1725
Size: 1104 977 2292
Fragments arranged by size:
2292 1104 977
AgeI A'CCGG_T
Cuts at: 2761 2761
Size: 4373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1032 1032
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 880 1678 3245 880
Size: 798 1567 2008
Fragments arranged by size:
2008 1567 798
ApaLI G'TGCA_C
Cuts at: 3143 3143
Size: 4373
ApoI r'AATT_y
Cuts at: 233 1377 3726 3982 233
Size: 1144 2349 256 624
Fragments arranged by size:
2349 1144 624 256
AseI AT'TA_AT
Cuts at: 20 601 20
Size: 581 3792
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 1393 1393
Size: 4373
BamHI G'GATC_C
Cuts at: 1398 1398
Size: 4373
BanI G'GyrC_C
Cuts at: 1389 1803 2051 2086 1389
Size: 414 248 35 3676
Fragments arranged by size:
3676 414 248 35
BanII G_rGCy'C
Cuts at: 1387 2417 1387
Size: 1030 3343
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BbsI GAAGACnn'nnnn_
Cuts at: 799 799
Size: 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4339
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3032 4373 2271
Size: 761 1341 2271
Fragments arranged by size:
2271 1341 761
BclI T'GATC_A
Cuts at: 267 1893 267
Size: 1626 2747
BglII A'GATC_T
Cuts at: 189 1056 1888 3925 189
Size: 867 832 2037 637
Fragments arranged by size:
2037 867 832 637
BlpI GC'TnA_GC
Cuts at: 1485 1485
Size: 4373
Bme1580I G_kGCm'C
Cuts at: 1998 2091 3147 1998
Size: 93 1056 3224
Fragments arranged by size:
3224 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 1585 1666 2001 4145 868
Size: 717 81 335 2144 1096
Fragments arranged by size:
2144 1096 717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1171 948
Size: 223 4150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1549 2428 2920 3218 808
Size: 741 879 492 298 1963
Fragments arranged by size:
1963 879 741 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 816 4077 816
Size: 3261 1112
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1562 2083 2761 3035 3182 3593 1562
Size: 521 678 274 147 411 2342
Fragments arranged by size:
2342 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 621 1137 621
Size: 516 3857
BseYI C'CCAG_C
Cuts at: 345 1798 2556 3133 3629 345
Size: 1453 758 577 496 1089
Fragments arranged by size:
1453 1089 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 1192
Size: 4373
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3169 104
Size: 1811 46 1208 1308
Fragments arranged by size:
1811 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1387 2165 2355 3147 1387
Size: 778 190 792 2613
Fragments arranged by size:
2613 792 778 190
BsmI GAATG_Cn'
Cuts at: 387 387
Size: 4373
Bsp1286I G_dGCh'C
Cuts at: 1387 1998 2091 2165 2355 2417 3147 1387
Size: 611 93 74 190 62 730 2613
Fragments arranged by size:
2613 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3593 3593
Size: 4373
BspHI T'CATG_A
Cuts at: 3549 3549
Size: 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1637 1939 2320 1423
Size: 214 302 381 3476
Fragments arranged by size:
3476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1026 2285 1026
Size: 1259 3114
BsrFI r'CCGG_y
Cuts at: 2371 2552 2761 2371
Size: 181 209 3983
Fragments arranged by size:
3983 209 181
BssSI C'ACGA_G
Cuts at: 2644 3002 2644
Size: 358 4015
BstAPI GCAn_nnn'nTGC
Cuts at: 679 1750 679
Size: 1071 3302
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstYI r'GATC_y
Cuts at: 1 189 710 1056 1398 1865 1888 2223 2469
Size: 188 521 346 342 467 23 335 246
Cuts at: 2469 3481 3567 3579 3925 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 521 467 449 346 346 342 335
246 188 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 624 1616 624
Size: 992 3381
DraI TTT'AAA
Cuts at: 1372 1372
Size: 4373
DrdI GACnn_nn'nnGTC
Cuts at: 914 2079 2937 914
Size: 1165 858 2350
Fragments arranged by size:
2350 1165 858
EaeI y'GGCC_r
Cuts at: 1958 2132 2523 2550 3895 1958
Size: 174 391 27 1345 2436
Fragments arranged by size:
2436 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 560 2396 2606 560
Size: 1836 210 2327
Fragments arranged by size:
2327 1836 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 2891 3037 4183 2581
Size: 310 146 1146 2771
Fragments arranged by size:
2771 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1385 1385
Size: 4373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 948 1171 2198 2630 3377 3645 181
Size: 767 223 1027 432 747 268 909
Fragments arranged by size:
1027 909 767 747 432 268 223
EcoO109I rG'GnC_Cy
Cuts at: 570 846 1353 1512 570
Size: 276 507 159 3431
Fragments arranged by size:
3431 507 276 159
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 664 2392 664
Size: 1728 2645
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 809 1807 2055 3077 4056 809
Size: 998 248 1022 979 1126
Fragments arranged by size:
1126 1022 998 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1255 1287 2215 2245 2247 2277 466
Size: 32 757 32 928 30 2 30 2562
Fragments arranged by size:
2562 928 757 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 1299 1412 1209
Size: 90 113 4170
Fragments arranged by size:
4170 113 90
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 924 3044 3228 924
Size: 2120 184 2069
Fragments arranged by size:
2120 2069 184
MscI TGG'CCA
Cuts at: 2134 2134
Size: 4373
MslI CAynn'nnrTG
Cuts at: 2362 2489 2362
Size: 127 4246
MspA1I CmG'CkG
Cuts at: 1484 1798 2158 3171 3416 1484
Size: 314 360 1013 245 2441
Fragments arranged by size:
2441 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NdeI CA'TA_TG
Cuts at: 321 321
Size: 4373
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NspI r_CATG'y
Cuts at: 1426 1926 2833 1426
Size: 500 907 2966
Fragments arranged by size:
2966 907 500
PciI A'CATG_T
Cuts at: 2829 2829
Size: 4373
PflMI CCAn_nnn'nTGG
Cuts at: 246 246
Size: 4373
PfoI T'CCnGG_A
Cuts at: 1148 1179 1148
Size: 31 4342
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 4341
PpuMI rG'GwC_Cy
Cuts at: 570 846 570
Size: 276 4097
PstI C_TGCA'G
Cuts at: 1420 2105 1420
Size: 685 3688
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 1798 2158 1798
Size: 360 4013
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1387 1387
Size: 4373
SalI G'TCGA_C
Cuts at: 1207 1410 1207
Size: 203 4170
SapI GCTCTTCn'nnn_
Cuts at: 2396 2606 2396
Size: 210 4163
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 333 3890 333
Size: 3557 816
SfcI C'TryA_G
Cuts at: 33 359 365 1416 2101 3094 3285 33
Size: 326 6 1051 685 993 191 1121
Fragments arranged by size:
1121 1051 993 685 326 191 6
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 787 1528 2443 2935 3197 787
Size: 741 915 492 262 1963
Fragments arranged by size:
1963 915 741 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 1426 1926 1426
Size: 500 3873
SspI AAT'ATT
Cuts at: 1134 1134
Size: 4373
StyI C'CwwG_G
Cuts at: 245 1507 1861 245
Size: 1262 354 2757
Fragments arranged by size:
2757 1262 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1956 2089 2548 2757 1956
Size: 133 459 209 3572
Fragments arranged by size:
3572 459 209 133
TatI w'GTAC_w
Cuts at: 331 3888 331
Size: 3557 816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4341
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1868 1868
Size: 4373
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI