Restriction map for
pF1KB7705
(Circular) (Six-base) MAPSORT of: pF1KB7705.seq Check: 3864 from: 1 to: 4370
pF1KB7705 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 320 1408 320
Size: 1088 3282
Acc65I G'GTAC_C
Cuts at: 597 1386 597
Size: 789 3581
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 2195 2627 3374 3642 217
Size: 1978 432 747 268 945
Fragments arranged by size:
1978 945 747 432 268
AflII C'TTAA_G
Cuts at: 352 624 702 352
Size: 272 78 4020
Fragments arranged by size:
4020 272 78
AflIII A'CryG_T
Cuts at: 1362 1722 2826 3803 1362
Size: 360 1104 977 1929
Fragments arranged by size:
1929 1104 977 360
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AleI CACnn'nnGTG
Cuts at: 422 422
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1194 1675 3242 1194
Size: 481 1567 2322
Fragments arranged by size:
2322 1567 481
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1390 1390
Size: 4370
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 597 1386 1800 2048 2083 597
Size: 789 414 248 35 2884
Fragments arranged by size:
2884 789 414 248 35
BanII G_rGCy'C
Cuts at: 1030 1384 2414 1030
Size: 354 1030 2986
Fragments arranged by size:
2986 1030 354
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 611 611
Size: 4370
BbvCI CC'TCA_GC
Cuts at: 1187 1187
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 255 270 1890 255
Size: 15 1620 2735
Fragments arranged by size:
2735 1620 15
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 1116 1995 2088 3144 1116
Size: 879 93 1056 2342
Fragments arranged by size:
2342 1056 879 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1049 2737 1049
Size: 1688 2682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 527
Size: 4370
Bpu10I CC'TnA_GC
Cuts at: 1187 1187
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2917 3215 1546
Size: 879 492 298 2701
Fragments arranged by size:
2701 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4074 4074
Size: 4370
BsaAI yAC'GTr
Cuts at: 183 1723 183
Size: 1540 2830
BsaBI GATnn'nnATC
Cuts at: 6 254 1884 3921 6
Size: 248 1630 2037 455
Fragments arranged by size:
2037 1630 455 248
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1110 1140 1110
Size: 30 4340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1020 1020
Size: 4370
BseYI C'CCAG_C
Cuts at: 1129 1158 1164 1795 2553 3130 3626 1129
Size: 29 6 631 758 577 496 1873
Fragments arranged by size:
1873 758 631 577 496 29 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1180
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3166 104
Size: 1808 46 1208 1308
Fragments arranged by size:
1808 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 693 1030 1126 1384 2162 2352 3144 693
Size: 337 96 258 778 190 792 1919
Fragments arranged by size:
1919 792 778 337 258 190 96
Bsp1286I G_dGCh'C
Cuts at: 693 1030 1116 1126 1384 1995 2088 2162 2352
Size: 337 86 10 258 611 93 74 190
Cuts at: 2352 2414 3144 693
Size: 62 730 1919
Fragments arranged by size:
1919 730 611 337 258 190 93 86
74 62 10
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 965 3546 965
Size: 2581 1789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1420 1634 1936 2317 1081
Size: 339 214 302 381 3134
Fragments arranged by size:
3134 381 339 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 1173 2368 2549 2758 1173
Size: 1195 181 209 2785
Fragments arranged by size:
2785 1195 209 181
BssSI C'ACGA_G
Cuts at: 1119 2641 2999 1119
Size: 1522 358 2490
Fragments arranged by size:
2490 1522 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 1395 1862 1885 2220 2466 3478 3564 3576
Size: 1394 467 23 335 246 1012 86 12
Cuts at: 3576 3922 1
Size: 346 449
Fragments arranged by size:
1394 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 716 107
Size: 609 3761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 555 1107 1200 1613 555
Size: 552 93 413 3312
Fragments arranged by size:
3312 552 413 93
ClaI AT'CG_AT
Cuts at: 438 438
Size: 4370
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 1054 1955 2129 2520 2547 3892 1054
Size: 901 174 391 27 1345 1532
Fragments arranged by size:
1532 1345 901 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 236 681 2393 2603 236
Size: 445 1712 210 2003
Fragments arranged by size:
2003 1712 445 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1382 1028
Size: 354 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 527 2195 2627 3374 3642 217
Size: 310 1668 432 747 268 945
Fragments arranged by size:
1668 945 747 432 310 268
EcoO109I rG'GnC_Cy
Cuts at: 335 831 1182 1509 335
Size: 496 351 327 3196
Fragments arranged by size:
3196 496 351 327
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 728 2389 728
Size: 1661 2709
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 289 294 321 680 712 2212 2242 2244
Size: 27 5 27 359 32 1500 30 2
Cuts at: 2244 2274 262
Size: 30 2358
Fragments arranged by size:
2358 1500 359 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
HindIII A'AGCT_T
Cuts at: 349 349
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 601 1390 601
Size: 789 3581
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3225 3041
Size: 184 4186
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 422 522 1268 2359 2486 422
Size: 100 746 1091 127 2306
Fragments arranged by size:
2306 1091 746 127 100
MspA1I CmG'CkG
Cuts at: 545 1481 1795 2155 3168 3413 545
Size: 936 314 360 1013 245 1502
Fragments arranged by size:
1502 1013 936 360 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NcoI C'CATG_G
Cuts at: 107 716 107
Size: 609 3761
NdeI CA'TA_TG
Cuts at: 491 1253 491
Size: 762 3608
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NspI r_CATG'y
Cuts at: 1366 1423 1923 2830 1366
Size: 57 500 907 2906
Fragments arranged by size:
2906 907 500 57
PciI A'CATG_T
Cuts at: 1362 2826 1362
Size: 1464 2906
PflMI CCAn_nnn'nTGG
Cuts at: 817 817
Size: 4370
PfoI T'CCnGG_A
Cuts at: 1178 1178
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PmlI CAC'GTG
Cuts at: 183 183
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 335 831 1182 335
Size: 496 351 3523
Fragments arranged by size:
3523 496 351
PstI C_TGCA'G
Cuts at: 550 1417 2102 550
Size: 867 685 2818
Fragments arranged by size:
2818 867 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1030 1384 1030
Size: 354 4016
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SanDI GG'GwC_CC
Cuts at: 831 831
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 236 681 2393 2603 236
Size: 445 1712 210 2003
Fragments arranged by size:
2003 1712 445 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 546 678 1077 1413 2098 3091 3282 33
Size: 513 132 399 336 685 993 191 1121
Fragments arranged by size:
1121 993 685 513 399 336 191 132
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 352 624 702 1525 2440 2932 3194 352
Size: 272 78 823 915 492 262 1528
Fragments arranged by size:
1528 915 823 492 272 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 107 293 716 826 1504 1858 107
Size: 186 423 110 678 354 2619
Fragments arranged by size:
2619 678 423 354 186 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 290 1953 2086 2545 2754 100
Size: 190 1663 133 459 209 1716
Fragments arranged by size:
1716 1663 459 209 190 133
TatI w'GTAC_w
Cuts at: 1220 3885 1220
Size: 2665 1705
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1110 1142 3887 3919 1110
Size: 32 2745 32 1561
Fragments arranged by size:
2745 1561 32 32
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1865 1865
Size: 4370
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI