Restriction map for
pF1KB7702
(Circular) (Six-base) MAPSORT of: pF1KB7702.seq Check: 1815 from: 1 to: 4496
pF1KB7702 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 585 941 585
Size: 356 4140
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1221 2321 2753 3500 3768 1221
Size: 1100 432 747 268 1949
Fragments arranged by size:
1949 1100 747 432 268
AfeI AGC'GCT
Cuts at: 298 298
Size: 4496
AflIII A'CryG_T
Cuts at: 193 248 1848 2952 3929 193
Size: 55 1600 1104 977 760
Fragments arranged by size:
1600 1104 977 760 55
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AhdI GACnn_n'nnGTC
Cuts at: 165 165
Size: 4496
AleI CACnn'nnGTG
Cuts at: 951 951
Size: 4496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 969 1093 1199 1459 1474 969
Size: 124 106 260 15 3991
Fragments arranged by size:
3991 260 124 106 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1237 1269 1237
Size: 32 4464
AlwNI CAG_nnn'CTG
Cuts at: 798 1167 1362 1365 1801 3368 798
Size: 369 195 3 436 1567 1926
Fragments arranged by size:
1926 1567 436 369 195 3
ApaI G_GGCC'C
Cuts at: 420 479 606 770 834 1392 420
Size: 59 127 164 64 558 3524
Fragments arranged by size:
3524 558 164 127 64 59
ApaLI G'TGCA_C
Cuts at: 252 3266 252
Size: 3014 1482
ApoI r'AATT_y
Cuts at: 1351 1500 3849 4105 1351
Size: 149 2349 256 1742
Fragments arranged by size:
2349 1742 256 149
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 489 631 1512 1926 2174 2209 489
Size: 142 881 414 248 35 2776
Fragments arranged by size:
2776 881 414 248 142 35
BanII G_rGCy'C
Cuts at: 420 479 554 606 755 770 785 834 860
Size: 59 75 52 149 15 15 49 26
Cuts at: 860 1132 1392 1510 2540 420
Size: 272 260 118 1030 2376
Fragments arranged by size:
2376 1030 272 260 149 118 75 59
52 49 26 15 15
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 954 954
Size: 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 471 505 471
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 1123 1123
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 148 256 420 479 494 606 629 636 770
Size: 108 164 59 15 112 23 7 134
Cuts at: 770 834 948 1392 2121 2214 3270 148
Size: 64 114 444 729 93 1056 1374
Fragments arranged by size:
1374 1056 729 444 164 134 114 112
108 93 64 59 23 15 7
BmrI ACTGGGnnnn_n'
Cuts at: 598 951 1708 1789 2124 4268 598
Size: 353 757 81 335 2144 826
Fragments arranged by size:
2144 826 757 353 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 2863 774
Size: 2089 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 782 826 994 1032 487
Size: 295 44 168 38 3951
Fragments arranged by size:
3951 295 168 44 38
Bpu10I CC'TnA_GC
Cuts at: 548 779 1286 1393 548
Size: 231 507 107 3651
Fragments arranged by size:
3651 507 231 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1672 2551 3043 3341 1204
Size: 468 879 492 298 2359
Fragments arranged by size:
2359 879 492 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 194 251 522 1849 194
Size: 57 271 1327 2841
Fragments arranged by size:
2841 1327 271 57
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 513 1685 2206 2884 3158 3305 3716 513
Size: 1172 521 678 274 147 411 1293
Fragments arranged by size:
1293 1172 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1402 1432 1402
Size: 30 4466
BseYI C'CCAG_C
Cuts at: 532 580 606 1921 2679 3256 3752 532
Size: 48 26 1315 758 577 496 1276
Fragments arranged by size:
1315 1276 758 577 496 48 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 924 1123 250
Size: 674 199 3623
Fragments arranged by size:
3623 674 199
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 256 785 1510 2288 2478 3270 256
Size: 529 725 778 190 792 1482
Fragments arranged by size:
1482 792 778 725 529 190
BsiWI C'GTAC_G
Cuts at: 119 737 119
Size: 618 3878
Bsp1286I G_dGCh'C
Cuts at: 148 256 420 479 494 554 606 629 636
Size: 108 164 59 15 60 52 23 7
Cuts at: 636 755 770 785 834 860 948 1132 1392
Size: 119 15 15 49 26 88 184 260
Cuts at: 1392 1510 2121 2214 2288 2478 2540 3270 148
Size: 118 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 260 190 184 164 119
118 108 93 88 74 62 60 59
52 49 26 23 15 15 15 7
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 585 941 1466 1546 1760 2062 2443 585
Size: 356 525 80 214 302 381 2638
Fragments arranged by size:
2638 525 381 356 302 214 80
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 568 882 1199 2494 2675 2884 568
Size: 314 317 1295 181 209 2180
Fragments arranged by size:
2180 1295 317 314 209 181
BsrGI T'GTAC_A
Cuts at: 401 401
Size: 4496
BssHII G'CGCG_C
Cuts at: 357 357
Size: 4496
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 798 1365 1873 798
Size: 567 508 3421
Fragments arranged by size:
3421 567 508
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstEII G'GTnAC_C
Cuts at: 1083 1083
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 561 561
Size: 4496
BstYI r'GATC_y
Cuts at: 1 1521 1988 2011 2346 2592 3604 3690 3702
Size: 1520 467 23 335 246 1012 86 12
Cuts at: 3702 4048 1
Size: 346 449
Fragments arranged by size:
1520 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 617 636 107
Size: 510 19 3967
Fragments arranged by size:
3967 510 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 610 1338 1739 610
Size: 728 401 3367
Fragments arranged by size:
3367 728 401
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 1325 1325
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 378 563 620 675 911 980 1115 2081 2255
Size: 185 57 55 236 69 135 966 174
Cuts at: 2255 2646 2673 4018 378
Size: 391 27 1345 856
Fragments arranged by size:
1345 966 856 391 236 185 174 135
69 57 55 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 1240 2519 2729 1240
Size: 1279 210 3007
Fragments arranged by size:
3007 1279 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 783 1508 783
Size: 725 3771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 782 826 994 1032 1221 2321 2753 3500
Size: 295 44 168 38 189 1100 432 747
Cuts at: 3500 3768 487
Size: 268 1215
Fragments arranged by size:
1215 1100 747 432 295 268 189 168
44 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1378 1378
Size: 4496
EcoO109I rG'GnC_Cy
Cuts at: 129 417 475 476 558 602 603 766 1389
Size: 288 58 1 82 44 1 163 623
Cuts at: 1389 1635 129
Size: 246 2990
Fragments arranged by size:
2990 623 288 246 163 82 58 44
1 1
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 231 2515 231
Size: 2284 2212
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 300 1930 2178 3200 4179 300
Size: 1630 248 1022 979 617
Fragments arranged by size:
1630 1022 979 617 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 2338 2368 2370 2400 151
Size: 32 2155 30 2 30 2247
Fragments arranged by size:
2247 2155 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 1117 2257 1117
Size: 1140 3356
MslI CAynn'nnrTG
Cuts at: 622 951 1273 1468 2485 2612 622
Size: 329 322 195 1017 127 2506
Fragments arranged by size:
2506 1017 329 322 195 127
MspA1I CmG'CkG
Cuts at: 795 804 1179 1194 1362 1607 1921 2281 3294
Size: 9 375 15 168 245 314 360 1013
Cuts at: 3294 3539 795
Size: 245 1752
Fragments arranged by size:
1752 1013 375 360 314 245 245 168
15 9
NaeI GCC'GGC
Cuts at: 884 2677 884
Size: 1793 2703
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 617 107
Size: 510 3986
NgoMIV G'CCGG_C
Cuts at: 882 2675 882
Size: 1793 2703
NspI r_CATG'y
Cuts at: 1401 1549 2049 2956 1401
Size: 148 500 907 2941
Fragments arranged by size:
2941 907 500 148
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 580 649 727 1272 580
Size: 69 78 545 3804
Fragments arranged by size:
3804 545 78 69
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 194 251 194
Size: 57 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 129 129
Size: 4496
PspOMI G'GGCC_C
Cuts at: 416 475 602 766 830 1388 416
Size: 59 127 164 64 558 3524
Fragments arranged by size:
3524 558 164 127 64 59
PstI C_TGCA'G
Cuts at: 794 803 854 1004 1178 1193 1543 2228 794
Size: 9 51 150 174 15 350 685 3062
Fragments arranged by size:
3062 685 350 174 150 51 15 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 795 804 1179 1194 1362 1921 2281 795
Size: 9 375 15 168 559 360 3010
Fragments arranged by size:
3010 559 375 360 168 15 9
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 785 1510 785
Size: 725 3771
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 854 1543 854
Size: 689 3807
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SexAI A'CCwGG_T
Cuts at: 821 821
Size: 4496
SfcI C'TryA_G
Cuts at: 33 790 799 850 1000 1174 1189 1539 2224
Size: 757 9 51 150 174 15 350 685
Cuts at: 2224 3217 3408 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 757 685 350 191 174 150
51 15 9
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1219 1651 2566 3058 3320 1219
Size: 432 915 492 262 2395
Fragments arranged by size:
2395 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1401 1549 2049 1401
Size: 148 500 3848
Fragments arranged by size:
3848 500 148
StyI C'CwwG_G
Cuts at: 107 554 617 1406 1630 1984 107
Size: 447 63 789 224 354 2619
Fragments arranged by size:
2619 789 447 354 224 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1135 2079 2212 2671 2880 1135
Size: 944 133 459 209 2751
Fragments arranged by size:
2751 944 459 209 133
TatI w'GTAC_w
Cuts at: 401 4011 401
Size: 3610 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 4013 4045 127
Size: 32 3854 32 578
Fragments arranged by size:
3854 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BbvCI BfrBI BmgBI
BmtI BseRI BsmI BsmBI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI